##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527733_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1135558
Sequences flagged as poor quality	0
Sequence length	52
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.777179148929424	31.0	31.0	34.0	30.0	34.0
2	31.667488582705595	31.0	31.0	34.0	30.0	34.0
3	32.49953414973079	34.0	31.0	34.0	30.0	34.0
4	36.133963214560595	37.0	35.0	37.0	35.0	37.0
5	35.07789474425789	35.0	35.0	37.0	33.0	37.0
6	35.74766150209853	37.0	35.0	37.0	35.0	37.0
7	36.3085073593775	37.0	35.0	37.0	35.0	37.0
8	36.55352874974242	37.0	37.0	37.0	35.0	37.0
9	38.58027242994193	39.0	39.0	39.0	37.0	39.0
10	37.78879634505679	39.0	38.0	39.0	35.0	39.0
11	37.457587371142644	39.0	37.0	39.0	35.0	39.0
12	37.1198371197244	39.0	37.0	39.0	34.0	39.0
13	36.77890429198685	39.0	37.0	39.0	33.0	39.0
14	37.738134908124465	40.0	38.0	40.0	33.0	40.0
15	37.8859978970691	40.0	38.0	40.0	33.0	40.0
16	37.96526201215614	40.0	38.0	40.0	34.0	40.0
17	38.022170598067206	40.0	38.0	40.0	34.0	40.0
18	38.00782522777348	40.0	38.0	40.0	34.0	40.0
19	37.94243270709202	40.0	38.0	40.0	34.0	40.0
20	37.971927457690406	40.0	38.0	40.0	34.0	40.0
21	38.01147717685931	40.0	38.0	40.0	34.0	40.0
22	37.918672582113814	40.0	38.0	40.0	34.0	40.0
23	37.84241403785628	40.0	38.0	40.0	33.0	40.0
24	37.727287377659266	40.0	37.0	40.0	33.0	40.0
25	37.40905264196105	39.0	37.0	40.0	33.0	40.0
26	36.938483106983526	39.0	35.0	40.0	32.0	40.0
27	36.748535081431335	39.0	35.0	40.0	32.0	40.0
28	36.63346478119127	39.0	35.0	40.0	31.0	40.0
29	36.50656769623392	39.0	35.0	40.0	31.0	40.0
30	36.278355663030865	39.0	35.0	40.0	31.0	40.0
31	36.114532238776	39.0	35.0	40.0	30.0	40.0
32	35.870446071446814	38.0	35.0	40.0	30.0	40.0
33	35.489945031429485	38.0	35.0	40.0	27.0	40.0
34	35.349031049052535	38.0	35.0	40.0	26.0	40.0
35	35.19197698400258	38.0	35.0	40.0	25.0	40.0
36	34.76883523342709	38.0	35.0	40.0	23.0	40.0
37	34.43401569977051	38.0	34.0	40.0	21.0	40.0
38	33.86396643764564	38.0	33.0	40.0	16.0	40.0
39	33.65865944319885	38.0	33.0	40.0	15.0	40.0
40	33.3189726988846	38.0	33.0	40.0	12.0	40.0
41	32.86353317047654	38.0	32.0	40.0	10.0	40.0
42	32.72449403729268	37.0	32.0	40.0	10.0	40.0
43	32.58239121207371	37.0	32.0	40.0	10.0	40.0
44	32.37706484389172	36.0	32.0	40.0	10.0	40.0
45	32.17135012038135	36.0	31.0	40.0	10.0	40.0
46	31.847469702120016	35.0	31.0	40.0	9.0	40.0
47	31.58206802294555	35.0	30.0	39.0	9.0	40.0
48	31.466171697086367	35.0	30.0	39.0	9.0	40.0
49	31.34199662192508	35.0	30.0	39.0	9.0	40.0
50	31.11763291703286	35.0	29.0	39.0	9.0	40.0
51	31.191359666349054	35.0	30.0	39.0	9.0	40.0
52	30.844066969718853	35.0	29.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	6.0
14	8.0
15	35.0
16	100.0
17	267.0
18	631.0
19	1329.0
20	2266.0
21	3729.0
22	5760.0
23	9168.0
24	13278.0
25	17723.0
26	23973.0
27	30183.0
28	34819.0
29	33389.0
30	28219.0
31	27382.0
32	33270.0
33	41697.0
34	63019.0
35	79268.0
36	120371.0
37	189125.0
38	280942.0
39	95597.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.4291793109643	0.3862418300078023	0.1498822605274235	0.034696598500472894
8	98.57620658742222	1.1199780196167874	0.19822853610295554	0.10558685685803806
9	96.69255115106405	1.8860331220422029	0.7317988160886542	0.6896169108050844
10	53.48013927954363	31.273787864644518	6.2108672564501335	9.035205599361722
11	30.033868811632697	35.508534130357056	17.938757861773684	16.518839196236566
12	31.54713365587667	27.959822395685645	22.661193880013176	17.831850068424508
13	22.902573008159866	30.403907154015915	24.685837271191787	22.00768256663244
14	23.70781589315561	33.30133731610363	23.551152825307028	19.439693965433733
15	23.219157453868494	31.947553537555983	23.41174999427594	21.42153901429958
16	25.264319391876068	32.98660218148258	22.293533223314	19.45554520332735
17	25.901274967901244	32.73720937195635	20.20389975677156	21.157615903370854
18	24.647794300247103	33.93943770375446	21.449895117642605	19.96287287835584
19	25.694680500687767	31.312359210185654	22.09970780884816	20.893252480278417
20	23.509675419485397	32.67292379605445	22.456800973618257	21.360599810841894
21	24.470612685569563	31.324599888336834	24.513675215180555	19.691112210913047
22	23.97755112464533	32.38927470019145	22.776027292309156	20.857146882854067
23	23.652776872691664	33.679037090135424	22.53183016631471	20.136355870858203
24	22.9245005539127	32.73738549682182	25.02848819699214	19.309625752273334
25	25.440268132495213	33.69066133125741	20.133361748144964	20.735708788102414
26	25.421951146484812	32.31160363451273	22.510695182456555	19.755750036545912
27	25.32226447262051	32.27276810167336	21.527213933590357	20.87775349211577
28	27.68867816527205	32.54100627180646	20.37333187736778	19.39698368555371
29	28.514439597096757	32.02161404349228	20.338283029136335	19.12566333027463
30	27.714480458065548	32.00629118019511	20.567421479131845	19.711806882607494
31	28.198295463551844	32.876083828390975	19.04394139268976	19.881679315367425
32	27.70276815451082	33.06506580905599	20.856354320959387	18.3758117154738
33	28.069636249315316	34.09750977052691	19.05829556922676	18.774558410931014
34	24.797588498341785	35.24064820995493	20.680317517907497	19.28144577379579
35	26.20024692706141	36.84391286046155	19.611856021444964	17.343984191032074
36	27.09408061939593	35.49145001840505	18.91994948738858	18.494519874810443
37	25.95428855241212	35.00340801614713	19.199811898643663	19.84249153279709
38	26.513220813027605	34.1314138071327	20.00769665662168	19.34766872321801
39	26.65975670111082	33.38490856477608	18.74135887378716	21.213975860325938
40	25.757204827934814	33.60999614286545	20.29319506357227	20.33960396562747
41	26.05423941357465	32.843764915574546	20.65055241564059	20.451443255210215
42	24.922108778239423	32.12077234276013	21.08417183446376	21.872947044536694
43	25.787586367230915	31.471664151016505	22.234443330943908	20.50630615080868
44	26.693396550418385	31.63061684211639	20.898712351108443	20.777274256356787
45	26.04860341787914	32.095674549428566	21.267165569702296	20.588556462990002
46	25.879347422148406	33.25563291351036	20.504721027019315	20.360298637321918
47	25.781333934506208	33.08708141724156	21.052469358676525	20.079115289575697
48	25.79498361158127	32.70489045913991	20.843321080913523	20.656804848365297
49	25.117695441360105	33.456767509893815	20.3513162691822	21.07422077956388
50	23.72983150134119	33.89294073926651	20.612773631994138	21.76445412739816
51	22.914989811176532	33.53118026556107	20.845610704164823	22.708219219097572
52	23.927267475549467	32.524714721749135	22.0350699832153	21.512947819486104
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	23.5
2	47.0
3	85.5
4	124.0
5	153.0
6	182.0
7	2971.5
8	5761.0
9	5670.5
10	5580.0
11	5576.0
12	5572.0
13	5336.0
14	5322.5
15	5545.0
16	5784.0
17	6023.0
18	7208.0
19	8393.0
20	9503.0
21	10613.0
22	13056.5
23	15500.0
24	19353.0
25	23206.0
26	27190.5
27	31175.0
28	36040.0
29	40905.0
30	40960.5
31	41016.0
32	41820.5
33	42625.0
34	42749.0
35	42873.0
36	41996.5
37	41120.0
38	39826.5
39	38309.5
40	38086.0
41	39299.5
42	40513.0
43	43211.5
44	45910.0
45	48122.5
46	50335.0
47	54754.0
48	59173.0
49	62590.0
50	66007.0
51	63648.5
52	61290.0
53	62580.5
54	63871.0
55	66027.0
56	68183.0
57	62662.0
58	57141.0
59	58875.0
60	60609.0
61	51953.5
62	43298.0
63	39879.5
64	30368.0
65	24275.0
66	21350.0
67	18425.0
68	15932.0
69	13439.0
70	12093.0
71	10747.0
72	7694.0
73	4641.0
74	3226.0
75	1811.0
76	1396.5
77	982.0
78	637.0
79	292.0
80	185.0
81	78.0
82	82.5
83	87.0
84	47.0
85	7.0
86	5.0
87	3.0
88	1.5
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1135558.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.211923279052222
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.11898721748996	20.199537589227226
2	8.10095559336128	4.084813418136671
3	3.2704194226268193	2.473606907207689
4	1.667826664038104	1.6819647158594515
5	0.9638640922004764	1.2150433771995865
6	0.6356595094182991	0.9615719269832479
7	0.4495549402502413	0.7933901264315593
8	0.35731893815129545	0.7206958123858294
9	0.2772501516028971	0.6291008596186054
>10	2.4777524424424455	14.602224162567978
>50	1.04608848944611	18.70591438967217
>100	0.6053249371905235	25.662556600543496
>500	0.01776103109121895	3.077503618312001
>1k	0.01123657069036301	5.192076495854505
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3977	0.3502242950161947	No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAA	3325	0.292807588868204	No Hit
TATGTAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT	3124	0.2751070398869983	No Hit
TATGTAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG	2782	0.2449896878891259	No Hit
TATGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2760	0.2430523143687949	No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAA	2429	0.213903649131088	No Hit
TATGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATGTAGGGAAG	2319	0.2042167815294331	No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	2212	0.19479410122600518	No Hit
TATGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	2129	0.1874849193083929	No Hit
TATGTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1986	0.17489199142624154	No Hit
TATGTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	1955	0.17216205601122972	No Hit
TATGTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	1868	0.16450062436264815	No Hit
TATGTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1808	0.15921687839810914	No Hit
TATGTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1794	0.15798400433971668	No Hit
TATGTAGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAA	1773	0.15613469325212803	No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGGAAAAAAAAAAAAAAAAAAAAA	1711	0.15067482242210437	No Hit
TATGTAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	1455	0.12813083964007121	Illumina Single End Adapter 2 (100% over 34bp)
TATGTAGGGGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAA	1453	0.1279547147745866	No Hit
TATGTAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC	1416	0.12469640476312085	No Hit
TATGTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1381	0.12161421961713977	No Hit
TATGTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1375	0.12108584502068588	No Hit
TATGTAGGGAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAA	1351	0.11897234663487026	No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	1339	0.11791559744196245	No Hit
TATGTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1321	0.11633047365260075	No Hit
TATGTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	1202	0.10585104415626503	No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGGAAAAAAAAAAAAAAAAAAA	1200	0.10567491929078039	No Hit
TATGTAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	1160	0.10215242198108772	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.1581601292052013	0.0	0.0	0.0	0.0
7	0.1663499354502368	0.0	0.0	0.0	0.0
8	0.233629634065367	0.0	0.0	0.0	0.0
9	0.3405374274145398	0.0	0.0	0.0	0.0
10	0.5402630248741147	0.0	0.0	0.0	0.0
11	0.7746852208341626	0.0	0.0	0.0	0.0
12	0.9044892466963378	0.0	0.0	0.0	0.0
13	1.004263982993383	0.0	0.0	0.0	0.0
14	1.1290484501892462	0.0	0.0	0.0	0.0
15	1.2721499033955113	0.0	0.0	0.0	0.0
16	1.3853101294693886	0.0	0.0	0.0	0.0
17	1.490104424432746	0.0	0.0	0.0	0.0
18	1.5903194728935026	0.0	0.0	0.0	0.0
19	1.6950257054241176	0.0	0.0	0.0	0.0
20	1.806600807708633	0.0	0.0	0.0	0.0
21	1.913860850788775	0.0	0.0	0.0	0.0
22	2.034946695809461	0.0	0.0	0.0	0.0
23	2.1642223470751825	0.0	0.0	0.0	0.0
24	2.30019074322932	0.0	0.0	0.0	0.0
25	2.4339575785648995	0.0	0.0	0.0	0.0
26	2.574945533385349	0.0	0.0	0.0	0.0
27	2.6962955657042618	0.0	0.0	0.0	0.0
28	2.820023283707217	0.0	0.0	0.0	0.0
29	2.915306835934404	0.0	0.0	0.0	0.0
30	3.0066275786881866	0.0	0.0	0.0	0.0
31	3.0774297746130097	0.0	0.0	0.0	0.0
32	3.1637309587004805	0.0	0.0	0.0	0.0
33	3.2432513354667925	0.0	0.0	0.0	0.0
34	3.3237403989932703	0.0	0.0	0.0	0.0
35	3.4196403882496536	0.0	0.0	0.0	0.0
36	3.4852469006426796	0.0	0.0	0.0	0.0
37	3.5566655335966986	0.0	0.0	0.0	0.0
38	3.628964790878141	0.0	0.0	0.0	0.0
39	3.7031133592471717	0.0	0.0	0.0	0.0
40	3.778054489510884	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTAA	25	3.417129E-5	46.0	25
GGTACGT	20	6.3114235E-4	46.0	8
CGAACGA	20	6.3114235E-4	46.0	27
CATGCTA	20	6.3114235E-4	46.0	45
TAAACGT	20	6.3114235E-4	46.0	43
GTTAGCT	20	6.3114235E-4	46.0	10
TTCGTAC	25	3.417129E-5	46.0	17
CCGCTAT	30	1.8613828E-6	46.0	15
ATCCGTA	20	6.3114235E-4	46.0	17
GTAATCC	30	1.8613828E-6	46.0	27
CGTTACA	30	1.8613828E-6	46.0	42
TACGCTT	60	0.0	46.0	11
TAACCCG	30	1.8613828E-6	46.0	18
TAATTCG	20	6.3114235E-4	46.0	35
CATAGCG	20	6.3114235E-4	46.0	38
CCATAGA	30	1.8613828E-6	46.0	28
TTGCGAT	25	3.417129E-5	46.0	40
CTCGATA	30	1.8613828E-6	46.0	24
TTACGGC	20	6.3114235E-4	46.0	44
TTACGCT	30	1.8613828E-6	46.0	45
>>END_MODULE