FastQCFastQC Report
Fri 17 Jun 2016
SRR1527732_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527732_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144865
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC17301.1942153039036345No Hit
GGTACAGGGGCCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGA4200.2899251026818072No Hit
GGTACAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAA4180.288544506954751No Hit
GGTACAGGGGAGCAAAAGCCCACTTCGCCATCATATTCGTAGGAGTAAAC4130.2850930176371104No Hit
GGTACAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4060.28026093259241364No Hit
GGTACAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA3960.27335795395713247No Hit
GGTACAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC3820.26369378386773895No Hit
GGTACAGGGGCTCTGGCTGGGCACAGTGGCTACAGCAGTCATTCCTTCAG3780.2609325924136265No Hit
GGTACAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT3660.25264901805128914No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC3460.2388430607807269No Hit
GGTACAGGGGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT3430.23677216719014252No Hit
GGTACAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA3340.23055948641838953No Hit
GGTACAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA3250.22434680564663653No Hit
GGTACAGGGGACACATGAAACCAAACACTTCATCTACTTCTACCTGGGTC3230.2229662099195803No Hit
GGTACAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT3230.2229662099195803No Hit
GGTACAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG3230.2229662099195803No Hit
GGTACAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC3200.22089531632899598No Hit
GGTACAGGGGGCACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTC3060.21123114623960237No Hit
GGTACAGGGAACGCCCTTGCTGCTTCTGTGTGTGGGTGATTGGATGTGGG2890.19949608255962448No Hit
GGTACAGGGAGTGAGCTTGTGCCATCCCCCTAAAATGGAAACAAGGCCCC2880.19880578469609636No Hit
GGTACAGGGGGCTTCCTCGGCGTCTCGGAGCGAGCGCGTGAGCGGTGGAC2860.19742518896904016No Hit
GGTACAGGGGACTGCCACTCCCGGGAGCATGAAGGACCGAACCCAGGAGC2800.19328340178787146No Hit
GGTACAGGGGGGACCCGTCTTTCCGTAGCGGGCTGAGAGAAGCGCGTCGC2790.19259310392434334No Hit
GGTACAGGGGCTTTCGGCCGCCGCAGCCATCTTCCAGTAACTCGCCAAAA2690.18569012528906223No Hit
GGTACAGGGGGAGACAGGCATGCGCATGGAGACAGTCACACCCATGGAGA2640.18223863597142165No Hit
GGTACAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCA2580.178096848790253No Hit
GGTACAGGGGGGCATCGCCTCGCCCTGTCTTGTGTGTGTGTGAGTGTGTG2570.1774065509267249No Hit
GGTACAGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCC2520.1739550616090843No Hit
GGTACAGGGCTCTCTTGATCTGGAAGCCAACTGCAAAGGCAGCACATAGT2450.16912297656438754No Hit
GGTACAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT2440.16843267870085943No Hit
GGTACAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAG2390.16498118938321885No Hit
GGTACAGGGCCGACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTT2360.16291029579263452No Hit
GGTACAGGGATCTTTCCCTGTAAAGATAACAGGGTATATTGTCAGCCTGT2350.1622199979291064No Hit
GGTACAGGGGCCCTTTTCCCTTTCTGCCACCGCCATGCCATCCAGACTGA2330.1608394022020502No Hit
GGTACAGGGGTGCACTCCACTTCTGTCACTGCAGACACTCCACTCTCCAC2320.16014910433852209No Hit
GGTACAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC2320.16014910433852209No Hit
GGTACAGGGACTTCGATCCCAGCAAGATCCAGTTACCAGAAGATGACTGA2310.15945880647499397No Hit
GGTACAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCG2300.15876850861146585No Hit
GGTACAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2300.15876850861146585No Hit
GGTACAGAGAGTTCGAGGCGCCGCCGCCGCCGCTGCAGCCGCCGGAGCCG2290.15807821074793774No Hit
GGTACAGGGGTGCTGATACAGGGGACTTTTTTTTTTTTTTTTTTTTTTTT2240.15462672143029715No Hit
GGTACAGGTTGGCGTACAGCAATATTTCGATCCTGACCGCGTGATCCAGA2240.15462672143029715No Hit
GGTACAGGGGGGAAACGCTTCGGGCCCCGGGCCGGTGTATCTGTTTCAAC2240.15462672143029715No Hit
GGTACAGGGGAGTCCAGGCGGCAGCGGGAATTGACAGGAGGACTACGCCG2230.15393642356676907No Hit
GGTACAGGGGGCAAAGGGTTTTTGTGAATTACGGTGGTGGGTAAGACCCG2220.15324612570324095No Hit
GGTACAGGGCTTTCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGA2220.15324612570324095No Hit
GGTACAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCAC2150.14841404065854416No Hit
GGTACAGGGAGGCTTGGGACGCCGCGGATCTGCGGCGGCGACAGTTGGAG2140.14772374279501604No Hit
GGTACAGGGGATGTGCCAGCATGCAGCGAGCTGGCCTGGCTGGACCTTGT2120.1463431470679598No Hit
GGTACAGGGACAAGACTCTGCAGACAGTGGGGCCATCCCATGCTAGAACC2120.1463431470679598No Hit
GGTACAGGGGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTCG2110.14565284920443172No Hit
GGTACAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTT2110.14565284920443172No Hit
GGTACAGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGAC2100.1449625513409036No Hit
GGTACAGGGGAAAAAAGGCGTAACCCGAGGCCACCGAAAACCCGAGATGC2080.14358195561384737No Hit
GGTACAGGGGAAAACATGGCGGAGCGCCCGAGGAAGCGTCCGTGTGGTCC2070.14289165775031926No Hit
GGTACAGGGACACCTGGCATGCGGAGTTGGCTCAGTGGTAGAGGGCTTGC2070.14289165775031926No Hit
GGTACAGGGGAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGG2050.14151106202326305No Hit
GGTACAGGGACTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTA2040.14082076415973493No Hit
GGTACAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG2030.14013046629620682No Hit
GGTACAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAG2000.13805957270562247No Hit
GGTACAGGGAACTCCCGTCAGCAGATCAGGAAGCTGATCAAGGATGGGCT1980.13667897697856624No Hit
GGTACAGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTC1980.13667897697856624No Hit
GGTACAGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGT1980.13667897697856624No Hit
GGTACAGGGATGTGGGGTAATTATTTGTGACCCAAGTGTAATTTGGATGA1960.13529838125151003No Hit
GGTACAGGGGGTTTTGGTTTTGTTTCGCTTTTCCATTTTAAGTCAGGGTC1950.1346080833879819No Hit
GGTACAGGGGATTGCCACTGCTCTTTCAAATTCAAAATGTTATCTGTGTA1940.1339177855244538No Hit
GGTACAGGGAGTTAAGAAAAACTATTTGCAGCCTGAAGTGGTGGCACACG1940.1339177855244538No Hit
GGTACAGGGGCCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGG1930.13322748766092568No Hit
GGTACAGGGGGTGACCTGCCTGGGCGCAGGGAACGGAAAGTCGGAAGGGG1920.1325371897973976No Hit
GGTACAGGAGTACTAAGTCCATTCCTTGAGCCCCCTGTGTCGTCAAACTG1900.13115659407034136No Hit
GGTACAGGGGTAGAACTCTCAGCTCCTTCTGCACCATGCCTGCCTGGATG1880.12977599834328513No Hit
GGTACAGGGATTGTTTTGAGCTGGTACCCTAGGATGAGGCTGGAGATGGT1860.1283954026162289No Hit
GGTACAGGGCTAGATCACAAGTTTGATCTGATGTATGCCAAGCGTGCCTT1860.1283954026162289No Hit
GGTACAGGGATGGCCTCCAAGGAGTAAGAAACCCTGGACCACCCACCCCA1840.1270148068891727No Hit
GGTACAGGGGGGCCTGTGAGCAGCTCAGGCCTTACATCCTGTCTTTAACA1840.1270148068891727No Hit
GGTACAGGGGTGCAGTGCTGGTACAGGGTGCAGTGTTGGTACAGGGATGT1820.12563421116211645No Hit
GGTACAGGGGCTCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAG1770.1221827218444759No Hit
GGTACAGGGGCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA1760.12149242398094778No Hit
GGTACAGGGAACTTTAACTGTTACTCCTTTTTAGCGCTAATTATTTGTCT1750.12080212611741967No Hit
GGTACAGGGGAGGAGGCGGCGCTGTGCTGTGGTGCGGACCTCGGGGCGGC1740.12011182825389156No Hit
GGTACAGGGATCTTCATACTCCTCAAGCAGACATCACTCTTCCGTTTCTT1720.11873123252683533No Hit
GGTACAGGGGGCCGCGCAGCCCCGCCTCTCCTTCCTGCGGCGCCTTAGGG1720.11873123252683533No Hit
GGTACAGGGAACGTACACTATGAGAACTACAGAAGCAGAAAGCTAGCAGC1710.11804093466330721No Hit
GGTACAGGGGAGACGCCCAGGTCGCCCGCATCCAGTGCCGCAGGACACCG1710.11804093466330721No Hit
GGTACAGGGGGCGCAACTGGTCTCGGCCGCCAAGGCTCCAGCACGCCGGC1690.116660338936251No Hit
GGTACAGGGGGTCGTCACTTCCGCCTTTCTCTCTTTCCCCGGCCTGGCTT1680.11597004107272288No Hit
GGTACAGGGGTCCCTGCTTCTCTTCTGACTGGGAAATAAACACCCCTGAA1680.11597004107272288No Hit
GGTACAGGGTTACCTGTTGCTGTCAGGGTTACCTATCTGCTAAACTATGT1680.11597004107272288No Hit
GGTACAGGAGTTTTTGTTTTTTTTAATAAAAAGCAGGATTCATGTAAATA1680.11597004107272288No Hit
GGTACAGGGGTTTGCAGAGAGAAAAGAATTTTTCTAATGATCTGTAAAAT1670.11527974320919476No Hit
GGTACAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGT1670.11527974320919476No Hit
GGTACAGGGGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCA1650.11389914748213853No Hit
GGTACAGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGT1640.11320884961861041No Hit
GGTACAGGGAACATTACAACATTTAGTGGCTTTCTGGGAAGATATCTCTT1640.11320884961861041No Hit
GGTACAGCTAATGGCCGTCTTCATTTCCATGCGGTGCACTTTATGCGGAC1620.11182825389155421No Hit
GGTACAGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAA1620.11182825389155421No Hit
GGTACAGGGGGAGCTCCCGGCGTGCCCTGCACTCTCTGCTGCCCGCCGCC1610.1111379560280261No Hit
GGTACAGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTTGG1600.11044765816449799No Hit
GGTACAGGGAGCATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCT1590.10975736030096987No Hit
GGTACAGGGGACTTGGAGCAGCTCTGCCGCGGGGACTGCACTGTCCGCCC1580.10906706243744176No Hit
GGTACAGGGGCTTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGG1560.10768646671038554No Hit
GGTACAGGGGCTCTCCGCCCTCTCGGTCTTAGCGCCATCTTCCTTGAGAC1560.10768646671038554No Hit
GGTACAGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAA1540.1063058709833293No Hit
GGTACAGGGACAAAGCACTGCAAGAAAGAAAACTGCTAATTTAGGATGGC1540.1063058709833293No Hit
GGTACAGCTCATTATCAGAAAGGTTAAAGGTTTTTTCCTACTAAAACCAT1530.10561557311980119No Hit
GGTACAGGGATGACAAACATACGAAAAACACACCCATTATTTAAAATTAT1530.10561557311980119No Hit
GGTACAGGGGTCCGACCACTTCCCAGTTGGCTGTATGAAAATTGGCACTT1530.10561557311980119No Hit
GGTACAGGGGAGTCCTGTGTGGAATTGGCCGGGGCTGCGGGAGTTGGAGT1530.10561557311980119No Hit
GGTACAGGGATCAGAAACCCTACTCACGGGCTGGTGAGATGGCTCAGTGG1520.10492527525627307No Hit
GGTACAGGGCAGATCTGTATTGTTCTTACTGAAACACAGCATGGAATTAA1520.10492527525627307No Hit
GGTACAGGGGAAAGGGCAGCAGCAGCAACGAGCCCTCCTCCGACTCCCTG1520.10492527525627307No Hit
GGTACAGGGGGTCGGAGAGCCGCGCGGCCTGAGGCTTCCTACCCATCCCG1510.10423497739274495No Hit
GGTACAGGGGACTGCAAACCAGCTGAACATCAAACCAGAAAAGTGAACAC1500.10354467952921685No Hit
GGTACAGGGGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGC1500.10354467952921685No Hit
GGTACAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA1490.10285438166568875No Hit
GGTACAGGGGACACACCTCCCCACACACTATTTGCAGATGACCAGTGTCC1490.10285438166568875No Hit
GGTACAGGGGTAGCCCAGTGCCCAGTGCTGGGCCTGGCATAGAGTAGGTT1490.10285438166568875No Hit
GGTACAGGGGCCCTGCCTTCCGTTGGCTAACATGCCCCTGTCCACTATGT1480.10216408380216065No Hit
GGTACAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGA1470.10147378593863253No Hit
GGTACAGGGATCCTCTGCCCCTTCCCCTGTCCCTCACGTTGGGAATGTAA1470.10147378593863253No Hit
GGTACAGGGGGAGTGGGGAAGATAGCTTATTTTGGACGTGGATTCGGTAG1470.10147378593863253No Hit
GGTACAGGGATCTGGCCAGTTTTTTGCCAAGACAGCAACAAGCATTGTGA1470.10147378593863253No Hit
GGTACAGGGGAGCAAACACGCAGTAAAACTCCACCTAAGCAAAACGCACA1460.10078348807510441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGACT650.044.00000415
CGGACTT650.044.00000416
TCACGGG408.22547E-944.024
TGATCCA254.4235385E-544.042
TATTTCG207.8336906E-444.023
AACGTTT207.8336906E-444.034
ATCCTGT254.4235385E-544.036
TTGATCC502.7284841E-1144.017
CTTCGGT351.4344005E-744.024
ATGGGTA207.8336906E-444.013
TTTAGCG254.4235385E-544.030
AACGTGT207.8336906E-444.026
CAGCGTT207.8336906E-444.019
ATGGGGC254.4235385E-544.038
GACAGTC207.8336906E-444.031
AACGTAC302.5123663E-644.010
AAACTAA207.8336906E-444.028
CTATGAG302.5123663E-644.018
AGCAAGT207.8336906E-444.013
CAGCGCA207.8336906E-444.038
Produced by FastQC (version 0.11.5)