FastQCFastQC Report
Fri 17 Jun 2016
SRR1527731_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527731_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217491
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA27311.2556841432519048No Hit
GGTACAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACG6520.29978251973644887No Hit
GGTACAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT6260.2878280020782469No Hit
GGTACAGGGGAGCAAAAGCCCACTTCGCCATCATATTCGTAGGAGTAAACAT6110.2809311649677458No Hit
GGTACAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT6060.2786322192642454No Hit
GGTACAGGGGACACATGAAACCAAACACTTCATCTACTTCTACCTGGGTCAG5980.2749539061386448No Hit
GGTACAGGGGCCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGATG5950.2735745387165446No Hit
GGTACAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG5890.27081580387234416No Hit
GGTACAGGGGCTCTGGCTGGGCACAGTGGCTACAGCAGTCATTCCTTCAGTT5770.2652983341839432No Hit
GGTACAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC5440.25012529254084076No Hit
GGTACAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG5190.23863056402333888No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC5170.23771098574193872No Hit
GGTACAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG5130.23587182917913846No Hit
GGTACAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG4860.22345752238023642No Hit
GGTACAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA4540.20874426987783404No Hit
GGTACAGGGGCTTTCGGCCGCCGCAGCCATCTTCCAGTAACTCGCCAAAATG4470.20552574589293351No Hit
GGTACAGGGGGCACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTA4450.20460616761153336No Hit
GGTACAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA4420.20322680018943312No Hit
GGTACAGGGGACTGCCACTCCCGGGAGCATGAAGGACCGAACCCAGGAGCTC4130.18989291510913095No Hit
GGTACAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4070.1871341802649305No Hit
GGTACAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG3890.17885797573232914No Hit
GGTACAGGGGGGACCCGTCTTTCCGTAGCGGGCTGAGAGAAGCGCGTCGCTA3870.177938397450929No Hit
GGTACAGGGGCCCTTTTCCCTTTCTGCCACCGCCATGCCATCCAGACTGAGG3870.177938397450929No Hit
GGTACAGGGCTCTCTTGATCTGGAAGCCAACTGCAAAGGCAGCACATAGTCT3850.17701881916952886No Hit
GGTACAGGGCCGACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAA3700.17012198205902773No Hit
GGTACAGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT3650.16782303635552734No Hit
GGTACAGGGAACGCCCTTGCTGCTTCTGTGTGTGGGTGATTGGATGTGGGGG3630.1669034580741272No Hit
GGTACAGGGAGTGAGCTTGTGCCATCCCCCTAAAATGGAAACAAGGCCCCAG3570.16414472322992676No Hit
GGTACAGGTTGGCGTACAGCAATATTTCGATCCTGACCGCGTGATCCAGAAT3530.16230556666712645No Hit
GGTACAGAGAGTTCGAGGCGCCGCCGCCGCCGCTGCAGCCGCCGGAGCCGAG3520.16184577752642637No Hit
GGTACAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA3520.16184577752642637No Hit
GGTACAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG3500.1609261992450262No Hit
GGTACAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC3490.16046641010432616No Hit
GGTACAGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCT3480.16000662096362608No Hit
GGTACAGGGGTGCTGATACAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTT3450.15862725354152585No Hit
GGTACAGGGGGCTTCCTCGGCGTCTCGGAGCGAGCGCGTGAGCGGTGGACTG3430.15770767526012572No Hit
GGTACAGGGATCTTTCCCTGTAAAGATAACAGGGTATATTGTCAGCCTGTGA3390.1558685186973254No Hit
GGTACAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG3360.15448915127522517No Hit
GGTACAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA3350.15402936213452512No Hit
GGTACAGGGGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTCGGG3320.15264999471242488No Hit
GGTACAGGGGAAAACATGGCGGAGCGCCCGAGGAAGCGTCCGTGTGGTCCGG3300.15173041643102475No Hit
GGTACAGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCG3280.1508108381496246No Hit
GGTACAGGGGGAGACAGGCATGCGCATGGAGACAGTCACACCCATGGAGATA3270.15035104900892451No Hit
GGTACAGGGACACCTGGCATGCGGAGTTGGCTCAGTGGTAGAGGGCTTGCCT3260.14989125986822444No Hit
GGTACAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAA3260.14989125986822444No Hit
GGTACAGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCT3230.1485118924461242No Hit
GGTACAGGGGATGTGCCAGCATGCAGCGAGCTGGCCTGGCTGGACCTTGTTC3220.14805210330542412No Hit
GGTACAGGGCTTTCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGG3210.14759231416472407No Hit
GGTACAGGGGGCAAAGGGTTTTTGTGAATTACGGTGGTGGGTAAGACCCGAG3190.1466727358833239No Hit
GGTACAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCA3180.14621294674262383No Hit
GGTACAGGGGAGTCCAGGCGGCAGCGGGAATTGACAGGAGGACTACGCCGCA3140.14437379017982352No Hit
GGTACAGGGGGTGACCTGCCTGGGCGCAGGGAACGGAAAGTCGGAAGGGGTA3120.14345421189842336No Hit
GGTACAGGGACTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAAT3110.1429944227577233No Hit
GGTACAGGGAGGCTTGGGACGCCGCGGATCTGCGGCGGCGACAGTTGGAGAG3070.14115526619492302No Hit
GGTACAGGGGTGCACTCCACTTCTGTCACTGCAGACACTCCACTCTCCACCT3030.1393161096321227No Hit
GGTACAGGGGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT3020.13885632049142263No Hit
GGTACAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA3010.13839653135072255No Hit
GGTACAGGGACAAGACTCTGCAGACAGTGGGGCCATCCCATGCTAGAACCTA3010.13839653135072255No Hit
GGTACAGGGGATTGCCACTGCTCTTTCAAATTCAAAATGTTATCTGTGTAAA2940.13517800736582203No Hit
GGTACAGGGGAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGAA2910.13379863994372182No Hit
GGTACAGGGAGTTAAGAAAAACTATTTGCAGCCTGAAGTGGTGGCACACGCC2880.13241927252162158No Hit
GGTACAGGGATGTGGGGTAATTATTTGTGACCCAAGTGTAATTTGGATGATT2870.1319594833809215No Hit
GGTACAGGGGTAGAACTCTCAGCTCCTTCTGCACCATGCCTGCCTGGATGCT2850.13103990509952135No Hit
GGTACAGGGACTTCGATCCCAGCAAGATCCAGTTACCAGAAGATGACTGATG2830.13012032681812122No Hit
GGTACAGGGGCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCAGC2820.12966053767742114No Hit
GGTACAGGGGGGAAACGCTTCGGGCCCCGGGCCGGTGTATCTGTTTCAACCG2750.12644201369252062No Hit
GGTACAGGGATTGTTTTGAGCTGGTACCCTAGGATGAGGCTGGAGATGGTGT2730.12552243541112046No Hit
GGTACAGGGGTCCGACCACTTCCCAGTTGGCTGTATGAAAATTGGCACTTCC2710.1246028571297203No Hit
GGTACAGGGGCTTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTC2660.12230391142621994No Hit
GGTACAGGAGTACTAAGTCCATTCCTTGAGCCCCCTGTGTCGTCAAACTGCT2660.12230391142621994No Hit
GGTACAGGGGAAAAAAGGCGTAACCCGAGGCCACCGAAAACCCGAGATGCAG2650.12184412228551986No Hit
GGTACAGGGGGTTTTGGTTTTGTTTCGCTTTTCCATTTTAAGTCAGGGTCTT2630.12092454400411971No Hit
GGTACAGGGAACTCCCGTCAGCAGATCAGGAAGCTGATCAAGGATGGGCTGA2620.12046475486341963No Hit
GGTACAGGGGAGACGCCCAGGTCGCCCGCATCCAGTGCCGCAGGACACCGAA2620.12046475486341963No Hit
GGTACAGGGATGGCCTCCAAGGAGTAAGAAACCCTGGACCACCCACCCCAGC2620.12046475486341963No Hit
GGTACAGGGGAGGAGGCGGCGCTGTGCTGTGGTGCGGACCTCGGGGCGGCGT2610.12000496572271956No Hit
GGTACAGGGAACATTACAACATTTAGTGGCTTTCTGGGAAGATATCTCTTTG2600.11954517658201949No Hit
GGTACAGGGAACTTTAACTGTTACTCCTTTTTAGCGCTAATTATTTGTCTGA2590.11908538744131941No Hit
GGTACAGGGGAAAGGGCAGCAGCAGCAACGAGCCCTCCTCCGACTCCCTGAG2580.11862559830061933No Hit
GGTACAGCTCATTATCAGAAAGGTTAAAGGTTTTTTCCTACTAAAACCATCC2570.11816580915991926No Hit
GGTACAGGAGTTTTTGTTTTTTTTAATAAAAAGCAGGATTCATGTAAATAGC2560.11770602001921918No Hit
GGTACAGGGACAAAGCACTGCAAGAAAGAAAACTGCTAATTTAGGATGGCCA2560.11770602001921918No Hit
GGTACAGGGATCTTCATACTCCTCAAGCAGACATCACTCTTCCGTTTCTTCT2560.11770602001921918No Hit
GGTACAGGGGCCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTC2550.11724623087851913No Hit
GGTACAGGGTTACCTGTTGCTGTCAGGGTTACCTATCTGCTAAACTATGTCG2550.11724623087851913No Hit
GGTACAGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTTGGTC2540.11678644173781905No Hit
GGTACAGGGATTTCCTGGACTTGCACTTGCCCCTTCCTTCCTTTGGGTTTTG2520.11586686345641888No Hit
GGTACAGGGATCAGAAACCCTACTCACGGGCTGGTGAGATGGCTCAGTGGGT2510.1154070743157188No Hit
GGTACAGGGGGCCGCGCAGCCCCGCCTCTCCTTCCTGCGGCGCCTTAGGGAC2480.11402770689361859No Hit
GGTACAGGGGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTG2480.11402770689361859No Hit
GGTACAGGGGGTCGTCACTTCCGCCTTTCTCTCTTTCCCCGGCCTGGCTTCC2430.11172876119011821No Hit
GGTACAGGGGGAGTGGGGAAGATAGCTTATTTTGGACGTGGATTCGGTAGAT2410.11080918290871807No Hit
GGTACAGGGGTGGGCTTGTTCCGATGTGAAGGGCCAGTGAGCTGTCTGGAAC2390.10988960462731791No Hit
GGTACAGGGGTGCAAAAGGCTGGCCAGGTTATTCGCATCATCTGTATCCTCA2390.10988960462731791No Hit
GGTACAGGGGGAGCTCCCGGCGTGCCCTGCACTCTCTGCTGCCCGCCGCCGA2390.10988960462731791No Hit
GGTACAGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAAT2390.10988960462731791No Hit
GGTACAGGGGCTCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTG2380.10942981548661783No Hit
GGTACAGGGAGCATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCTCA2380.10942981548661783No Hit
GGTACAGGGGAGTCCTGTGTGGAATTGGCCGGGGCTGCGGGAGTTGGAGTCG2380.10942981548661783No Hit
GGTACAGGGGACTTGGAGCAGCTCTGCCGCGGGGACTGCACTGTCCGCCCTG2370.10897002634591775No Hit
GGTACAGGGGTTTGCAGAGAGAAAAGAATTTTTCTAATGATCTGTAAAATTA2360.1085102372052177No Hit
GGTACAGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCG2340.10759065892381753No Hit
GGTACAGGGGTGCAGTGCTGGTACAGGGTGCAGTGTTGGTACAGGGATGTTT2340.10759065892381753No Hit
GGTACAGGGGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCACT2340.10759065892381753No Hit
GGTACAGGGAACGTACACTATGAGAACTACAGAAGCAGAAAGCTAGCAGCAG2330.10713086978311745No Hit
GGTACAGGGGGTCGGAGAGCCGCGCGGCCTGAGGCTTCCTACCCATCCCGCC2310.10621129150171732No Hit
GGTACAGGGGAAGGCACCGGCTTCCGGGAGCTGCGCAGCGAGCGGAGAGTCA2290.10529171322031716No Hit
GGTACAGGGCTAGATCACAAGTTTGATCTGATGTATGCCAAGCGTGCCTTTG2280.10483192407961708No Hit
GGTACAGGGGAGCAAACACGCAGTAAAACTCCACCTAAGCAAAACGCACAGC2280.10483192407961708No Hit
GGTACAGGGGGCGCAACTGGTCTCGGCCGCCAAGGCTCCAGCACGCCGGCCG2280.10483192407961708No Hit
GGTACAGGGACAAATTAAAAAAACCTCTATAATCACCCCCAATACTAAAAAA2270.10437213493891702No Hit
GGTACAGGGGGGGTGGGACTTATCCTATTAAAAGACAAGAAACTGCTACATT2270.10437213493891702No Hit
GGTACAGGGTATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCC2260.10391234579821694No Hit
GGTACAGGGCACGCTAATGTTTTAGCTAAAATTATTTGAAGAATCATTGTCA2260.10391234579821694No Hit
GGTACAGGGGTCCCTGCTTCTCTTCTGACTGGGAAATAAACACCCCTGAAGG2230.1025329783761167No Hit
GGTACAGGGGCCCTGCCTTCCGTTGGCTAACATGCCCCTGTCCACTATGTGC2230.1025329783761167No Hit
GGTACAGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGG2220.10207318923541664No Hit
GGTACAGGGGGTGGGTCGAGTCCCCATTCATTGCAGTGGCCTGCTGGGTTGG2220.10207318923541664No Hit
GGTACAGGGGTAGCCCAGTGCCCAGTGCTGGGCCTGGCATAGAGTAGGTTCT2220.10207318923541664No Hit
GGTACAGGGATCTGGCCAGTTTTTTGCCAAGACAGCAACAAGCATTGTGAAG2200.10115361095401648No Hit
GGTACAGGGGACACACCTCCCCACACACTATTTGCAGATGACCAGTGTCCTA2190.1006938218133164No Hit
GGTACAGGGATTAAAGATGTACCTAGTGCCCGGCATGGTGGCGCACGCCTTT2180.10023403267261635No Hit
GGTACAGGGGTGCTGTCTAATGCATGCCTTGAGTGTGTTTGCTGTTTAAAGA2180.10023403267261635No Hit
GGTACAGGGAAGGACAGGGCTTGCTTTGTTTTGTTTTCTGTTAGTTTTTGTT2180.10023403267261635No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAA206.3001143E-446.00000418
TCCGGAA206.3001143E-446.00000415
TATTTCG405.5752025E-946.00000423
TTTAGGT800.046.00000425
CTTAAAC206.3001143E-446.00000430
ATCCTCA206.3001143E-446.00000446
TTTAGCG405.5752025E-946.00000430
TTTAGCA206.3001143E-446.00000439
CGTGGTT206.3001143E-446.00000425
CAGGTCG206.3001143E-446.00000418
AGCAATC206.3001143E-446.00000429
AGGTAAT206.3001143E-446.00000432
TAATAAG206.3001143E-446.00000441
GTACCTG750.046.0000049
AATCCAC206.3001143E-446.00000423
TCTTGCC206.3001143E-446.00000423
CCCCTAA405.5752025E-946.00000427
ACACGTC206.3001143E-446.00000419
ACACGGT206.3001143E-446.00000439
CATATTA206.3001143E-446.00000427
Produced by FastQC (version 0.11.5)