FastQCFastQC Report
Fri 17 Jun 2016
SRR1527730_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527730_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217611
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA24541.1277003460303017No Hit
GGTACAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACG6270.2881288170175221No Hit
GGTACAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT5950.2734236780309819No Hit
GGTACAGGGGCCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGATG5570.25596132548446543No Hit
GGTACAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG5540.25458271870447724No Hit
GGTACAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT5510.2532041119244891No Hit
GGTACAGGGGACACATGAAACCAAACACTTCATCTACTTCTACCTGGGTCAG5300.2435538644645721No Hit
GGTACAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC5190.23849897293794892No Hit
GGTACAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG5190.23849897293794892No Hit
GGTACAGGGGAGCAAAAGCCCACTTCGCCATCATATTCGTAGGAGTAAACAT5150.23666083056463139No Hit
GGTACAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG5060.23252501022466696No Hit
GGTACAGGGGCTCTGGCTGGGCACAGTGGCTACAGCAGTCATTCCTTCAGTT4850.22287476276474996No Hit
GGTACAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA4730.21736033564479737No Hit
GGTACAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG4370.20081705428493965No Hit
GGTACAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA4330.19897891191162212No Hit
GGTACAGGGGGCACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTA4140.19024773563836386No Hit
GGTACAGGGGCTTTCGGCCGCCGCAGCCATCTTCCAGTAACTCGCCAAAATG4120.18932866445170513No Hit
GGTACAGGGGACTGCCACTCCCGGGAGCATGAAGGACCGAACCCAGGAGCTC4080.1874905220783876No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC3960.181976094958435No Hit
GGTACAGGGGGGACCCGTCTTTCCGTAGCGGGCTGAGAGAAGCGCGTCGCTA3690.1695686339385417No Hit
GGTACAGGGAGTGAGCTTGTGCCATCCCCCTAAAATGGAAACAAGGCCCCAG3690.1695686339385417No Hit
GGTACAGGGACAAGACTCTGCAGACAGTGGGGCCATCCCATGCTAGAACCTA3670.16864956275188295No Hit
GGTACAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG3650.16773049156522418No Hit
GGTACAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC3630.16681142037856542No Hit
GGTACAGGGGAGTCCAGGCGGCAGCGGGAATTGACAGGAGGACTACGCCGCA3620.16635188478523605No Hit
GGTACAGGGGCCCTTTTCCCTTTCTGCCACCGCCATGCCATCCAGACTGAGG3570.16405420681858915No Hit
GGTACAGGGAGGCTTGGGACGCCGCGGATCTGCGGCGGCGACAGTTGGAGAG3540.162675600038601No Hit
GGTACAGGGGTGCACTCCACTTCTGTCACTGCAGACACTCCACTCTCCACCT3530.16221606444527162No Hit
GGTACAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA3480.15991838647862472No Hit
GGTACAGGGGGCTTCCTCGGCGTCTCGGAGCGAGCGCGTGAGCGGTGGACTG3440.1580802441053072No Hit
GGTACAGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCG3440.1580802441053072No Hit
GGTACAGGGGGAGACAGGCATGCGCATGGAGACAGTCACACCCATGGAGATA3430.1576207085119778No Hit
GGTACAGGGCTCTCTTGATCTGGAAGCCAACTGCAAAGGCAGCACATAGTCT3430.1576207085119778No Hit
GGTACAGGGAACGCCCTTGCTGCTTCTGTGTGTGGGTGATTGGATGTGGGGG3370.1548634949520015No Hit
GGTACAGGGCTTTCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGG3320.1525658169853546No Hit
GGTACAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAA3270.1502681390187077No Hit
GGTACAGAGAGTTCGAGGCGCCGCCGCCGCCGCTGCAGCCGCCGGAGCCGAG3270.1502681390187077No Hit
GGTACAGGGATCTTTCCCTGTAAAGATAACAGGGTATATTGTCAGCCTGTGA3260.14980860342537833No Hit
GGTACAGGGGATGTGCCAGCATGCAGCGAGCTGGCCTGGCTGGACCTTGTTC3260.14980860342537833No Hit
GGTACAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG3260.14980860342537833No Hit
GGTACAGGGCCGACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAA3250.14934906783204893No Hit
GGTACAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3220.1479704610520608No Hit
GGTACAGGGACTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAAT3210.1475109254587314No Hit
GGTACAGGGGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTCGGG3190.14659185427207266No Hit
GGTACAGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCT3180.14613231867874327No Hit
GGTACAGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT3130.14383464071209634No Hit
GGTACAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG3130.14383464071209634No Hit
GGTACAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCA3060.14061789155879068No Hit
GGTACAGGGGGCAAAGGGTTTTTGTGAATTACGGTGGTGGGTAAGACCCGAG3040.1396988203721319No Hit
GGTACAGGGGGTGACCTGCCTGGGCGCAGGGAACGGAAAGTCGGAAGGGGTA3030.13923928477880254No Hit
GGTACAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA2980.13694160681215564No Hit
GGTACAGGTTGGCGTACAGCAATATTTCGATCCTGACCGCGTGATCCAGAAT2980.13694160681215564No Hit
GGTACAGGGGGGAAACGCTTCGGGCCCCGGGCCGGTGTATCTGTTTCAACCG2960.13602253562549688No Hit
GGTACAGGGGAAAACATGGCGGAGCGCCCGAGGAAGCGTCCGTGTGGTCCGG2950.1355630000321675No Hit
GGTACAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA2890.1328057864721912No Hit
GGTACAGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCT2890.1328057864721912No Hit
GGTACAGGGGCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCAGC2890.1328057864721912No Hit
GGTACAGGGACACCTGGCATGCGGAGTTGGCTCAGTGGTAGAGGGCTTGCCT2860.13142717969220308No Hit
GGTACAGGGGATTGCCACTGCTCTTTCAAATTCAAAATGTTATCTGTGTAAA2840.13050810850554428No Hit
GGTACAGGGGAAAAAAGGCGTAACCCGAGGCCACCGAAAACCCGAGATGCAG2840.13050810850554428No Hit
GGTACAGGGGAGGAGGCGGCGCTGTGCTGTGGTGCGGACCTCGGGGCGGCGT2830.13004857291221492No Hit
GGTACAGGAGTACTAAGTCCATTCCTTGAGCCCCCTGTGTCGTCAAACTGCT2820.12958903731888552No Hit
GGTACAGGGACTTCGATCCCAGCAAGATCCAGTTACCAGAAGATGACTGATG2820.12958903731888552No Hit
GGTACAGGGAACATTACAACATTTAGTGGCTTTCTGGGAAGATATCTCTTTG2800.12866996613222675No Hit
GGTACAGGGGTGCTGATACAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTT2800.12866996613222675No Hit
GGTACAGGGGTAGAACTCTCAGCTCCTTCTGCACCATGCCTGCCTGGATGCT2780.127750894945568No Hit
GGTACAGGGAACTCCCGTCAGCAGATCAGGAAGCTGATCAAGGATGGGCTGA2760.12683182375890925No Hit
GGTACAGGGGGTCGTCACTTCCGCCTTTCTCTCTTTCCCCGGCCTGGCTTCC2710.12453414579226235No Hit
GGTACAGGGATTGTTTTGAGCTGGTACCCTAGGATGAGGCTGGAGATGGTGT2690.12361507460560359No Hit
GGTACAGGGGGCCGCGCAGCCCCGCCTCTCCTTCCTGCGGCGCCTTAGGGAC2650.12177693223228606No Hit
GGTACAGGGGTGCAAAAGGCTGGCCAGGTTATTCGCATCATCTGTATCCTCA2620.12039832545229791No Hit
GGTACAGGGATCTTCATACTCCTCAAGCAGACATCACTCTTCCGTTTCTTCT2610.11993878985896853No Hit
GGTACAGGGGGAGTGGGGAAGATAGCTTATTTTGGACGTGGATTCGGTAGAT2600.11947925426563914No Hit
GGTACAGGGATGTGGGGTAATTATTTGTGACCCAAGTGTAATTTGGATGATT2570.11810064748565101No Hit
GGTACAGGGAGCATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCTCA2570.11810064748565101No Hit
GGTACAGGGACAAAGCACTGCAAGAAAGAAAACTGCTAATTTAGGATGGCCA2570.11810064748565101No Hit
GGTACAGGGAACTTTAACTGTTACTCCTTTTTAGCGCTAATTATTTGTCTGA2560.11764111189232161No Hit
GGTACAGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTTGGTC2560.11764111189232161No Hit
GGTACAGGGTTACCTGTTGCTGTCAGGGTTACCTATCTGCTAAACTATGTCG2540.11672204070566285No Hit
GGTACAGGGGAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGAA2530.11626250511233348No Hit
GGTACAGGGTATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCC2520.11580296951900411No Hit
GGTACAGGGGCTCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTG2520.11580296951900411No Hit
GGTACAGGGGGGCCTGTGAGCAGCTCAGGCCTTACATCCTGTCTTTAACATG2500.11488389833234534No Hit
GGTACAGGGGCCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTC2500.11488389833234534No Hit
GGTACAGGGATGGCCTCCAAGGAGTAAGAAACCCTGGACCACCCACCCCAGC2490.11442436273901595No Hit
GGTACAGGGGAGACGCCCAGGTCGCCCGCATCCAGTGCCGCAGGACACCGAA2470.11350529155235718No Hit
GGTACAGGGGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT2460.1130457559590278No Hit
GGTACAGGGGTGGGCTTGTTCCGATGTGAAGGGCCAGTGAGCTGTCTGGAAC2460.1130457559590278No Hit
GGTACAGCTCATTATCAGAAAGGTTAAAGGTTTTTTCCTACTAAAACCATCC2460.1130457559590278No Hit
GGTACAGGGGGAGCTCCCGGCGTGCCCTGCACTCTCTGCTGCCCGCCGCCGA2440.11212668477236903No Hit
GGTACAGGGATCAGAAACCCTACTCACGGGCTGGTGAGATGGCTCAGTGGGT2440.11212668477236903No Hit
GGTACAGGGGAGTCCTGTGTGGAATTGGCCGGGGCTGCGGGAGTTGGAGTCG2410.1107480779923809No Hit
GGTACAGGGCTAGATCACAAGTTTGATCTGATGTATGCCAAGCGTGCCTTTG2400.11028854239905153No Hit
GGTACAGGGGGCGCAACTGGTCTCGGCCGCCAAGGCTCCAGCACGCCGGCCG2390.10982900680572213No Hit
GGTACAGGGGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCACT2380.10936947121239277No Hit
GGTACAGGGATTAAAGATGTACCTAGTGCCCGGCATGGTGGCGCACGCCTTT2360.108450400025734No Hit
GGTACAGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCG2350.1079908644324046No Hit
GGTACAGGGATGACAAACATACGAAAAACACACCCATTATTTAAAATTATTA2340.10753132883907524No Hit
GGTACAGGAGTTTTTGTTTTTTTTAATAAAAAGCAGGATTCATGTAAATAGC2320.10661225765241647No Hit
GGTACAGGGGTGCAGTGCTGGTACAGGGTGCAGTGTTGGTACAGGGATGTTT2300.1056931864657577No Hit
GGTACAGGGGTCCGACCACTTCCCAGTTGGCTGTATGAAAATTGGCACTTCC2300.1056931864657577No Hit
GGTACAGGGGACTTGGAGCAGCTCTGCCGCGGGGACTGCACTGTCCGCCCTG2280.10477411527909895No Hit
GGTACAGGGGCTGAATGGTTTGGTGGTCACTGAGGTGTGGATGTGGTTTTAT2280.10477411527909895No Hit
GGTACAGGGGACCCCCTATGTTTACAGTCAGCAGGCGGTGGTATGCATTGTC2270.10431457968576956No Hit
GGTACAGGGGAAAGGGCAGCAGCAGCAACGAGCCCTCCTCCGACTCCCTGAG2270.10431457968576956No Hit
GGTACAGGGGCTTCCTACTCGTGTGGTCTTTGTGACTTCCACCATGGCGTAC2260.10385504409244019No Hit
GGTACAGGGGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTG2260.10385504409244019No Hit
GGTACAGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGG2250.10339550849911079No Hit
GGTACAGGGGCAAAGGGTTTTTGTGAATTACGGTGGTGGGTAAGACCCGAGT2240.10293597290578142No Hit
GGTACAGGGAACGTACACTATGAGAACTACAGAAGCAGAAAGCTAGCAGCAG2230.10247643731245203No Hit
GGTACAGGGATTTCCTGGACTTGCACTTGCCCCTTCCTTCCTTTGGGTTTTG2220.10201690171912266No Hit
GGTACAGGGGGTTTTGGTTTTGTTTCGCTTTTCCATTTTAAGTCAGGGTCTT2210.10155736612579329No Hit
GGTACAGGGGCTTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTC2210.10155736612579329No Hit
GGTACAGGGGTTTGCAGAGAGAAAAGAATTTTTCTAATGATCTGTAAAATTA2200.10109783053246389No Hit
GGTACAGGGAAGGACAGGGCTTGCTTTGTTTTGTTTTCTGTTAGTTTTTGTT2200.10109783053246389No Hit
GGTACAGGGGACGTAGCGCATCCGCTTGCCGGGAGGAACCAAGCTACGGCGG2190.10063829493913451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAC750.046.00000430
CGACCGG750.046.00000433
ACTTCGC750.046.00000422
TTCCAGT750.046.00000432
AGCGACC750.046.00000431
CCAGTAA750.046.00000434
CACTTCG750.046.00000421
AGTAACT750.046.00000436
TAACTCG750.046.00000438
CGGACTT750.046.00000416
TCACGGA206.300125E-446.039
GCGAGAC206.300125E-446.027
TGATCCA501.6370905E-1146.042
TCCGGAA301.8544051E-646.015
TATTTCG301.8544051E-646.023
AACGTTG206.300125E-446.037
ATGGGTA405.5752025E-946.013
CTTCGCT206.300125E-446.036
TTTAGCG253.407968E-546.030
CAGAATC453.074092E-1046.020
Produced by FastQC (version 0.11.5)