SRR1527729_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527729_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	141943
Sequences flagged as poor quality	0
Sequence length	50
%GC	59

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CATCCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1019	0.7178938024418252	No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	887	0.6248987269537772	No Hit
CATCCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	773	0.5445847981231903	No Hit
CATCCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCATCCCGGGA	705	0.49667824408389283	No Hit
CATCCCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	571	0.40227415230057134	No Hit
CATCCCGGGATGAGCACGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	523	0.3684577612140084	Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	489	0.34450448419435975	No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	437	0.30787006051724986	No Hit
CATCCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	430	0.3029385034837928	No Hit
CATCCCGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTC	426	0.3001204708932459	No Hit
CATCCCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	405	0.28532579979287465	No Hit
CATCCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	385	0.27123563684014007	No Hit
CATCCCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	368	0.2592589983303157	Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	367	0.25855449018267895	No Hit
CATCCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	358	0.2522139168539484	No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	346	0.2437598190823077	No Hit
CATCCCGGGATGAGCACTGCACGATCGGAAGAGCTCGTATGCCGTCTTCT	338	0.23812375390121387	Illumina Single End Adapter 2 (96% over 29bp)
CATCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAG	299	0.21064793614338148	No Hit
CATCCCGGGAGGTAGTGTTTGCAGGAACATAGTAAAGATGAAATTTGAGT	289	0.20360285466701425	No Hit
CATCCCGGGGAGCTCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGT	276	0.19444424874773678	No Hit
CATCCCGGAAACCAAGCCTGCCAAGACAGTCAACTATTAGTGTCACCCAG	273	0.1923307243048266	No Hit
CATCCCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	262	0.18458113468082257	No Hit
CATCCCGGGATGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA	258	0.18176310209027569	Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA	254	0.17894506949972877	No Hit
CATCCCGGGGAGCTGAGCAACGCCGAAGACAATGGCAGGCTCGGCGTTGG	246	0.17330900431863494	No Hit
CATCCCGGGAACAAACGCCAGGCGTGGTGGCGCACGCCTTTTAATCCCAG	246	0.17330900431863494	No Hit
CATCCCGGGGGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGTCGG	244	0.17189998802336148	No Hit
CATCCCGGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG	237	0.1669684309899044	No Hit
CATCCCGGGATAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA	236	0.16626392284226765	Illumina Single End Adapter 2 (100% over 34bp)
CATCCCGGGTGCAGCTGTCACTCACAGAGCACTGAGTTGCCTGGCCTTGG	233	0.1641503983993575	No Hit
CATCCCGGGATCCCTGTTTCTGTAACATCCTGGTCTGGACTGTCTACCCT	231	0.16274138210408404	No Hit
CATCCCGGGATGAGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA	227	0.15992334951353712	Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGGATCACGGGCGGCTCAACCTCCAAGGCCTGCTGCTAGAGAT	223	0.15710531692299023	No Hit
CATCCCGGGGCTCTGCGCCGCGCAAGTCCCCAAATCCAGCTCCTGTCCTA	222	0.15640080877535348	No Hit
CATCCCGGGGGCCGCCGCCGCTGCAGCCGCCGGAGCCGAGATGCCTAAAG	212	0.14935572729898622	No Hit
CATCCCGGGTGGGTGTGGTGGCGCATGCCTTTAATCCCAGCAGTTGGGAG	211	0.1486512191513495	No Hit
CATCCCGGGATGGCTGTTCACGTTGAAATAGTTTGTACGTGCTCGGTAAA	210	0.14794671100371276	No Hit
CATCCCGGGGCTCTTCCGGCGCGGGGAAGCTCTTCCGGTATTAGTTTCAA	209	0.14724220285607603	No Hit
CATCCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	208	0.1465376947084393	No Hit
CATCCCGGGAGGAACAGTTCTTTGTGGTTCTGGACGTCGGTGTCTGATGG	208	0.1465376947084393	No Hit
CATCCCGGGGCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA	204	0.1437196621178924	No Hit
CATCCCGGGGCGTCTGGCTCCCGCTCTCACAGCCATTGCAGTACATTGAG	203	0.14301515397025566	No Hit
CATCCCGGGGGGGTTATAGTTAGGCTATATAGTATATTCAGAATCCCAGG	203	0.14301515397025566	No Hit
CATCCCGGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGAT	201	0.1416061376749822	No Hit
CATCCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	200	0.1409016295273455	No Hit
CATCCCGTCAACATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAATT	200	0.1409016295273455	No Hit
CATCCCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	195	0.13737908878916186	No Hit
CATCCCGGAGGTTCGGAGCCGCCGCGATGGCGGAGGACAGCGAGTCTGCG	194	0.1366745806415251	No Hit
CATCCCGGGGGGCCAATGGCTGCTGCATTGTCCTGCACGCCACAGCTCGT	190	0.1338565480509782	No Hit
CATCCCGGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCG	189	0.13315203990334148	No Hit
CATCCCGGGATGAGCACTGCAGATCGGAAGAGCTCGTATGCCGTCTTCTG	184	0.12962949916515784	Illumina Single End Adapter 2 (100% over 30bp)
CATCCCGGGTCCTCCAGCGTTGAACACTTCCTTGCTTTTTTCACATGTTT	183	0.1289249910175211	No Hit
CATCCCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	183	0.1289249910175211	No Hit
CATCCCGGGGGACTGAACATGTGGTCTTAACAGCCTTTTTTATCAGATAT	182	0.12822048286988438	No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTG	179	0.1261069584269742	No Hit
CATCCCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	177	0.12469794213170075	No Hit
CATCCCGGGCTCATCCCGGGGGGATGAGTCTCCGGGTGGGTCAGGAGCAA	174	0.12258441768879055	No Hit
CATCCCGGGGGAAGTCCTGGCGACTGAGCATGTTGGTGCCCGGGCGTCTC	174	0.12258441768879055	No Hit
CATCCCGGGATTTCCAGCTGGGTCTTTATGGGAGGCTAGCGCTCCAGCTA	172	0.12117540139351711	No Hit
CATCCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	171	0.12047089324588038	No Hit
CATCCCGGGTGCTGAGGACTCCATCAAGCACGCTGCCTTGCCAGGATGAC	170	0.11976638509824367	No Hit
CATCCCGGGGGCCCGCCCTTAATAAGCTCGAGCTGCCGCGTCCGCCCTGC	167	0.11765286065533347	No Hit
CATCCCGGGGAATAAATGAATTCACTAATGTTCATGTGCAACCTCATGGG	166	0.11694835250769677	No Hit
CATCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	165	0.11624384436006002	No Hit
CATCCCGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTG	165	0.11624384436006002	No Hit
CATCCCGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTT	164	0.1155393362124233	No Hit
CATCCCGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA	164	0.1155393362124233	No Hit
CATCCCGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA	163	0.11483482806478656	No Hit
CATCCCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	162	0.11413031991714985	No Hit
CATCCCGGGGAGTCGGAAGCCGGAGCTGTCCCGGGACTCCCGATATGGCG	161	0.11342581176951312	No Hit
CATCCCGGGATGAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA	161	0.11342581176951312	Illumina Single End Adapter 2 (100% over 34bp)
CATCCCGGGCGTGGTGAGACACGACGCTGAGTTGCGGGTGCCCTCACCCA	159	0.11201679547423965	No Hit
CATCCCGGGGACACACCGCGCTTTGTACCGTCCTCCGGTCCACGCTCGCA	159	0.11201679547423965	No Hit
CATCCCGGGATGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA	159	0.11201679547423965	Illumina Single End Adapter 2 (100% over 34bp)
CATCCCGGGGATGCAAGGCGAGGACGCCAGATACCTCAAAAGGAAAGTTA	158	0.11131228732660294	No Hit
CATCCCGGGGAACCTGGGTCCTCTGGAAGAGCAGCTAGTGTTTTTAACTG	157	0.1106077791789662	No Hit
CATCCCGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGC	157	0.1106077791789662	No Hit
CATCCCGGGGAGAGCGCGGGACGTCTGTCTTCGAGTCCGAACGTTCGTGG	157	0.1106077791789662	No Hit
CATCCCGGGGTTAAAGTCTGACAAGTTGGGGCTCAAGCCTGTATCTTTTA	154	0.10849425473605603	No Hit
CATCCCGGGATGAGCACAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	153	0.10778974658841929	Illumina Single End Adapter 2 (96% over 26bp)
CATCCCGGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATC	151	0.10638073029314583	No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTGAGAGCACCCGACTG	151	0.10638073029314583	No Hit
CATCCCGGGGTCACCACTTGAACAGAATGGGTAACACTGCCCCAGCTATG	150	0.10567622214550912	No Hit
CATCCCGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGT	150	0.10567622214550912	No Hit
CATCCCGGGCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT	149	0.10497171399787239	No Hit
CATCCCGGGGTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCT	149	0.10497171399787239	No Hit
CATCCCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCT	146	0.1028581895549622	No Hit
CATCCCGGGGTATAAACCCTGATGTATGCTAAGTGTTCTGGATATGAATG	145	0.10215368140732548	No Hit
CATCCCGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAA	145	0.10215368140732548	No Hit
CATCCCGGGATTTTTGTTTAGCCCAGTAGGGGTGACTTAGGCAGTATTAT	145	0.10215368140732548	No Hit
CATCCCGGGGCACTTGACTTTATTGTAAACCCCTGGGCTCTGAGTAAGTC	143	0.10074466511205203	No Hit
CATCCCGGGATCACAAACACTTTACATGGACCTTCATGTCAAACTTGGTT	142	0.1000401569644153	No Hit
CATCCCGGGGCTCTCGAGTCCGGGCCGCAAGTCCCAGACGCTGCCCATGG	142	0.1000401569644153	No Hit
CATCCCGAAGTTGCGGCTCATTCTGATTCTGAACAGCTTCTTGGGAAGTA	142	0.1000401569644153	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCAT	20	7.8331446E-4	44.000004	31
GAAATTT	40	8.223651E-9	44.000004	40
AAGTATC	20	7.8331446E-4	44.000004	40
TATCAGC	20	7.8331446E-4	44.000004	36
TTCTAGT	20	7.8331446E-4	44.000004	39
GTGCACA	20	7.8331446E-4	44.000004	39
CAGGTAG	20	7.8331446E-4	44.000004	39
CGGGTCT	55	1.8189894E-12	44.000004	6
TGATATT	20	7.8331446E-4	44.000004	34
TGTCAAA	20	7.8331446E-4	44.000004	37
AGGTAGT	40	8.223651E-9	44.000004	10
AATCCCT	20	7.8331446E-4	44.000004	41
AGATCGC	20	7.8331446E-4	44.000004	19
CAAACAC	20	7.8331446E-4	44.000004	14
ACACGAC	40	8.223651E-9	44.000004	19
GCGACTT	20	7.8331446E-4	44.000004	43
ACGCCTC	20	7.8331446E-4	44.000004	23
GATCGCC	20	7.8331446E-4	44.000004	20
TCTCTCA	20	7.8331446E-4	44.000004	27
GAATGAA	20	7.8331446E-4	44.000004	9

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content