FastQCFastQC Report
Fri 17 Jun 2016
SRR1527728_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527728_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206892
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG15320.7404829572917271No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA12730.6152968698644704No Hit
CATCCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT11550.5582622817701989No Hit
CATCCCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC9060.43790963401194827No Hit
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC7900.38184173385147807No Hit
CATCCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCATCCCGGGAAG6620.31997370608820064No Hit
CATCCCGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG6340.30644007501498366No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT6250.30208997931287823No Hit
CATCCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG5960.28807300427276067No Hit
CATCCCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG5960.28807300427276067No Hit
CATCCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC5910.28565628443825763No Hit
CATCCCGGGATGAGCACGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA5190.2508555188214141Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT5180.25037217485451346No Hit
CATCCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT5090.246022079152408No Hit
CATCCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT5080.2455387351855074No Hit
CATCCCGGGAGGTAGTGTTTGCAGGAACATAGTAAAGATGAAATTTGAGTAT4290.20735456180035963No Hit
CATCCCGGGGAGCTCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGC4170.20155443419755237No Hit
CATCCCGGAAACCAAGCCTGCCAAGACAGTCAACTATTAGTGTCACCCAGGT4100.19817102642924808No Hit
CATCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA3890.1880208031243354No Hit
CATCCCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG3850.18608742725673297No Hit
CATCCCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA3710.1793206117201245No Hit
CATCCCGGGTGCAGCTGTCACTCACAGAGCACTGAGTTGCCTGGCCTTGGGA3690.1783539237863233No Hit
CATCCCGGGGAGCTGAGCAACGCCGAAGACAATGGCAGGCTCGGCGTTGGCA3660.17690389188562147No Hit
CATCCCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3600.17400382808421785Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGAACAAACGCCAGGCGTGGTGGCGCACGCCTTTTAATCCCAGCA3490.1686870444483112No Hit
CATCCCGGGGATCACGGGCGGCTCAACCTCCAAGGCCTGCTGCTAGAGATGC3450.16675366858070878No Hit
CATCCCGGGATCCCTGTTTCTGTAACATCCTGGTCTGGACTGTCTACCCTTA3410.16482029271310636No Hit
CATCCCGGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGT3260.15757013320959729No Hit
CATCCCGGGTGGGTGTGGTGGCGCATGCCTTTAATCCCAGCAGTTGGGAGGC3230.15612010130889545No Hit
CATCCCGGGGCTCTGCGCCGCGCAAGTCCCCAAATCCAGCTCCTGTCCTAGC3210.15515341337509425No Hit
CATCCCGGGATGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA3160.15273669354059122Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGGGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGTCGGAC3100.1498366297391876No Hit
CATCCCGGGGGGGTTATAGTTAGGCTATATAGTATATTCAGAATCCCAGGGG3090.149353285772287No Hit
CATCCCGGGGGGCCAATGGCTGCTGCATTGTCCTGCACGCCACAGCTCGTAT3070.14838659783848576No Hit
CATCCCGGGATAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA3070.14838659783848576Illumina Single End Adapter 2 (100% over 34bp)
CATCCCGGGGGCCGCCGCCGCTGCAGCCGCCGGAGCCGAGATGCCTAAAGGA3060.14790325387158518No Hit
CATCCCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA3030.14645322197088334No Hit
CATCCCGGGAGGAACAGTTCTTTGTGGTTCTGGACGTCGGTGTCTGATGGAG3010.14548653403708214No Hit
CATCCCGGGGCGTCTGGCTCCCGCTCTCACAGCCATTGCAGTACATTGAGCT3000.14500319007018156No Hit
CATCCCGGGATGAGCACTGCACGATCGGAAGAGCTCGTATGCCGTCTTCTGC3000.14500319007018156Illumina Single End Adapter 2 (96% over 31bp)
CATCCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC2950.14258647023567853No Hit
CATCCCGGGGGAAGTCCTGGCGACTGAGCATGTTGGTGCCCGGGCGTCTCCC2920.14113643833497672No Hit
CATCCCGGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGA2910.1406530943680761No Hit
CATCCCGGAGGTTCGGAGCCGCCGCGATGGCGGAGGACAGCGAGTCTGCGGC2860.13823637453357307No Hit
CATCCCGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA2860.13823637453357307No Hit
CATCCCGGGGAACCTGGGTCCTCTGGAAGAGCAGCTAGTGTTTTTAACTGCT2820.13630299866597065No Hit
CATCCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG2810.13581965469907004No Hit
CATCCCGGGGCTCTTCCGGCGCGGGGAAGCTCTTCCGGTATTAGTTTCAAGC2740.13243624693076583No Hit
CATCCCGGGATGGCTGTTCACGTTGAAATAGTTTGTACGTGCTCGGTAAAGA2740.13243624693076583No Hit
CATCCCGGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGATGC2660.12856949519556096No Hit
CATCCCGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTC2650.12808615122866038No Hit
CATCCCGGGGGACTGAACATGTGGTCTTAACAGCCTTTTTTATCAGATATCT2630.12711946329485915No Hit
CATCCCGGGGGCCAGTTGACGCAGCTGCGGTGCCGCTGGCCTCCACTCCTGC2590.12518608742725673No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTGAGAGCACCCGACTGCT2580.12470274346035612No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCT2570.12421939949345552No Hit
CATCCCGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA2560.12373605552655491No Hit
CATCCCGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACC2520.12180267965895249No Hit
CATCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC2490.12035264775825069No Hit
CATCCCGGGTCCTCCAGCGTTGAACACTTCCTTGCTTTTTTCACATGTTTTA2480.11986930379135007No Hit
CATCCCGGGGCACTTGACTTTATTGTAAACCCCTGGGCTCTGAGTAAGTCGT2450.11841927189064827No Hit
CATCCCGGGGTATAAACCCTGATGTATGCTAAGTGTTCTGGATATGAATGTA2450.11841927189064827No Hit
CATCCCGGGGCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCAGC2440.11793592792374766No Hit
CATCCCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC2430.11745258395684706No Hit
CATCCCGGGGGCCCGCCCTTAATAAGCTCGAGCTGCCGCGTCCGCCCTGCGC2410.11648589602304585No Hit
CATCCCGGGGTCACCACTTGAACAGAATGGGTAACACTGCCCCAGCTATGTC2400.11600255205614524No Hit
CATCCCGGGATGAGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA2390.11551920808924464Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT2380.11503586412234403No Hit
CATCCCGGGGTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCA2380.11503586412234403No Hit
CATCCCGGGGTTAAAGTCTGACAAGTTGGGGCTCAAGCCTGTATCTTTTAGG2320.1121358003209404No Hit
CATCCCGGGATTTCCAGCTGGGTCTTTATGGGAGGCTAGCGCTCCAGCTACG2310.1116524563540398No Hit
CATCCCGGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCT2310.1116524563540398No Hit
CATCCCGGGGCTCTTTCCGAGCTGCTAGCGCTCCTGCGAACATGGTGAACGT2300.11116911238713918No Hit
CATCCCGGGGACACACCGCGCTTTGTACCGTCCTCCGGTCCACGCTCGCAGT2290.11068576842023858No Hit
CATCCCGGGGGCGCTCGGGTTGCCTTGCTGGTGTTGGGGGGATAGTGTGTGT2290.11068576842023858No Hit
CATCCCGGGGAATAAATGAATTCACTAATGTTCATGTGCAACCTCATGGGAA2280.11020242445333797No Hit
CATCCCGGGATTTTTGTTTAGCCCAGTAGGGGTGACTTAGGCAGTATTATAT2250.10875239255263616No Hit
CATCCCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA2240.10826904858573555No Hit
CATCCCGGGGAAACTGTGCTCAACTTGGCTTATGCCCAGCTCATCCTTGGAA2220.10730236065193434No Hit
CATCCCGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCA2210.10681901668503374No Hit
CATCCCGGGGCTCTCGAGTCCGGGCCGCAAGTCCCAGACGCTGCCCATGGAG2190.10585232875123254No Hit
CATCCCGGGGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG2170.1048856408174313No Hit
CATCCCGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCG2170.1048856408174313No Hit
CATCCCGGGGAGAGCGCGGGACGTCTGTCTTCGAGTCCGAACGTTCGTGGTG2150.1039189528836301No Hit
CATCCCGGGACTGTGTATAAGGTATGTGTATAGATGAAAGCATACTGTTTTA2120.10246892098292829No Hit
CATCCCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG2110.10198557701602767No Hit
CATCCCGGGGAGAGACCCGGAGTCCGAATTCAGAGGCCCCAGTCTGTAGTCC2110.10198557701602767No Hit
CATCCCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGA2110.10198557701602767No Hit
CATCCCGGGGCTTCCTGTTCTCCACCATGGCGCAAGATCAAGGGGAAAAGGA2110.10198557701602767No Hit
CATCCCGGGGAGTCGGAAGCCGGAGCTGTCCCGGGACTCCCGATATGGCGAC2090.10101888908222646No Hit
CATCCCGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCG2080.10053554511532588No Hit
CATCCCGGGCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGG2080.10053554511532588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTAAA551.8189894E-1246.00000423
CGGGGAT5250.046.0000046
GCCTAAA551.8189894E-1246.00000443
ATAGTAA551.8189894E-1246.00000429
TAGTAAA551.8189894E-1246.00000430
TCTGCGA253.407379E-546.025
CTTAATA253.407379E-546.018
GTTGACG405.5733835E-946.015
GTGTTCG206.2994E-446.011
GTGTTAT206.2994E-446.09
TTAGGTG206.2994E-446.014
AGGTATG253.407379E-546.020
GATACTA206.2994E-446.019
TAATACT206.2994E-446.028
TAATAAG253.407379E-546.020
TCGTTCC206.2994E-446.013
CCTTAAT253.407379E-546.017
GGTACGG206.2994E-446.020
ACCGGTT206.2994E-446.044
TACCCGT453.074092E-1046.035
Produced by FastQC (version 0.11.5)