FastQCFastQC Report
Fri 17 Jun 2016
SRR1527727_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527727_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208069
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG13840.6651639600324892No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA12720.6113356626888196No Hit
CATCCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT10880.5229034599099337No Hit
CATCCCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC8660.41620808481801713No Hit
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC7280.3498839327338527No Hit
CATCCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCATCCCGGGAAG6370.3061484411421211No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT5820.27971490226799767No Hit
CATCCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC5690.2734669748977503No Hit
CATCCCGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG5620.2701027063137709No Hit
CATCCCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG5440.26145172995496685No Hit
CATCCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT5410.2600099005618329No Hit
CATCCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT5290.2542425829892968No Hit
CATCCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG5230.2513589242030288No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT4820.23165392249686403No Hit
CATCCCGGGATGAGCACGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA4740.22780904411517333Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGGAGCTCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGC4400.2114683109929879No Hit
CATCCCGGGTGCAGCTGTCACTCACAGAGCACTGAGTTGCCTGGCCTTGGGA3910.18791843090513244No Hit
CATCCCGGAAACCAAGCCTGCCAAGACAGTCAACTATTAGTGTCACCCAGGT3870.18599599171428707No Hit
CATCCCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG3870.18599599171428707No Hit
CATCCCGGGAGGTAGTGTTTGCAGGAACATAGTAAAGATGAAATTTGAGTAT3750.18022867414175103No Hit
CATCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA3700.17782562515319436No Hit
CATCCCGGGGAGCTGAGCAACGCCGAAGACAATGGCAGGCTCGGCGTTGGCA3570.171577697782947No Hit
CATCCCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA3420.16436855081727697No Hit
CATCCCGGGAACAAACGCCAGGCGTGGTGGCGCACGCCTTTTAATCCCAGCA3330.16004306263787493No Hit
CATCCCGGGGATCACGGGCGGCTCAACCTCCAAGGCCTGCTGCTAGAGATGC3210.1542757450653389No Hit
CATCCCGGGATCCCTGTTTCTGTAACATCCTGGTCTGGACTGTCTACCCTTA3150.1513920862790709No Hit
CATCCCGGGGGCCGCCGCCGCTGCAGCCGCCGGAGCCGAGATGCCTAAAGGA3120.14995025688593686No Hit
CATCCCGGGTGGGTGTGGTGGCGCATGCCTTTAATCCCAGCAGTTGGGAGGC3090.14850842749280288No Hit
CATCCCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3070.14754720789738018Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGATGAGCACTGCACGATCGGAAGAGCTCGTATGCCGTCTTCTGC3050.14658598830195754Illumina Single End Adapter 2 (96% over 31bp)
CATCCCGGGGCGTCTGGCTCCCGCTCTCACAGCCATTGCAGTACATTGAGCT3010.14466354911111218No Hit
CATCCCGGGGGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGTCGGAC3000.14418293931340084No Hit
CATCCCGGGAGGAACAGTTCTTTGTGGTTCTGGACGTCGGTGTCTGATGGAG2980.14322171971797817No Hit
CATCCCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA2940.1412992805271328No Hit
CATCCCGGGATGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA2920.14033806093171017Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGATAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA2880.1384156217408648Illumina Single End Adapter 2 (100% over 34bp)
CATCCCGGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGT2880.1384156217408648No Hit
CATCCCGGGGCTCTTCCGGCGCGGGGAAGCTCTTCCGGTATTAGTTTCAAGC2810.13505135315688546No Hit
CATCCCGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTC2780.13360952376375146No Hit
CATCCCGGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGA2770.13312891396604012No Hit
CATCCCGGAGGTTCGGAGCCGCCGCGATGGCGGAGGACAGCGAGTCTGCGGC2760.13264830416832876No Hit
CATCCCGGGGAACCTGGGTCCTCTGGAAGAGCAGCTAGTGTTTTTAACTGCT2730.1312064747751948No Hit
CATCCCGGGGGGCCAATGGCTGCTGCATTGTCCTGCACGCCACAGCTCGTAT2720.13072586497748342No Hit
CATCCCGGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGATGC2650.12736159639350408No Hit
CATCCCGGGGGAAGTCCTGGCGACTGAGCATGTTGGTGCCCGGGCGTCTCCC2640.12688098659579275No Hit
CATCCCGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA2630.12640037679808142No Hit
CATCCCGGGGGGGTTATAGTTAGGCTATATAGTATATTCAGAATCCCAGGGG2620.12591976700037005No Hit
CATCCCGGGATGGCTGTTCACGTTGAAATAGTTTGTACGTGCTCGGTAAAGA2610.12543915720265875No Hit
CATCCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG2590.12447793760723606No Hit
CATCCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC2540.12207488861867939No Hit
CATCCCGGGATTTTTGTTTAGCCCAGTAGGGGTGACTTAGGCAGTATTATAT2510.12063305922554537No Hit
CATCCCGGGGCTCTGCGCCGCGCAAGTCCCCAAATCCAGCTCCTGTCCTAGC2470.11871062003470004No Hit
CATCCCGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACC2470.11871062003470004No Hit
CATCCCGGGGCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCAGC2460.11823001023698869No Hit
CATCCCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT2440.11726879064156602No Hit
CATCCCGGGGCACTTGACTTTATTGTAAACCCCTGGGCTCTGAGTAAGTCGT2390.11486574165300933No Hit
CATCCCGGGATGAGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA2380.114385131855298Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCT2340.11246269266445266No Hit
CATCCCGGGTCCTCCAGCGTTGAACACTTCCTTGCTTTTTTCACATGTTTTA2330.11198208286674133No Hit
CATCCCGGGGGCGCTCGGGTTGCCTTGCTGGTGTTGGGGGGATAGTGTGTGT2320.11150147306902998No Hit
CATCCCGGGGTATAAACCCTGATGTATGCTAAGTGTTCTGGATATGAATGTA2310.11102086327131865No Hit
CATCCCGGGGGACTGAACATGTGGTCTTAACAGCCTTTTTTATCAGATATCT2300.1105402534736073No Hit
CATCCCGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCG2290.11005964367589599No Hit
CATCCCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC2280.10957903387818464No Hit
CATCCCGGGGGCCCGCCCTTAATAAGCTCGAGCTGCCGCGTCCGCCCTGCGC2280.10957903387818464No Hit
CATCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC2280.10957903387818464No Hit
CATCCCGGGATTTCCAGCTGGGTCTTTATGGGAGGCTAGCGCTCCAGCTACG2230.10717598488962797No Hit
CATCCCGGGGAGAGCGCGGGACGTCTGTCTTCGAGTCCGAACGTTCGTGGTG2230.10717598488962797No Hit
CATCCCGGGGGACGCTGTTGAGCTGGGAGGCGCGGTTGGAAGTCGTTACTTT2220.10669537509191664No Hit
CATCCCGGGGAATAAATGAATTCACTAATGTTCATGTGCAACCTCATGGGAA2210.10621476529420529No Hit
CATCCCGGGGAAACTGTGCTCAACTTGGCTTATGCCCAGCTCATCCTTGGAA2210.10621476529420529No Hit
CATCCCGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCG2210.10621476529420529No Hit
CATCCCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA2210.10621476529420529No Hit
CATCCCGGGGCTCTCGAGTCCGGGCCGCAAGTCCCAGACGCTGCCCATGGAG2200.10573415549649395No Hit
CATCCCGGGGAGAGACCCGGAGTCCGAATTCAGAGGCCCCAGTCTGTAGTCC2170.10429232610335995No Hit
CATCCCGGGGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG2160.10381171630564862No Hit
CATCCCGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG2160.10381171630564862No Hit
CATCCCGGGGATGCAAGGCGAGGACGCCAGATACCTCAAAAGGAAAGTTAAA2140.10285049671022593No Hit
CATCCCGGGGTTAAAGTCTGACAAGTTGGGGCTCAAGCCTGTATCTTTTAGG2130.1023698869125146No Hit
CATCCCGGGGCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTCTGCGCTAC2130.1023698869125146No Hit
CATCCCGGGCTCATCCCGGGGGGATGAGTCTCCGGGTGGGTCAGGAGCAAAC2120.10188927711480325No Hit
CATCCCGGGGTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCA2120.10188927711480325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATA750.046.00000419
AACTAGA750.046.00000417
AGCTAGT750.046.00000433
CGGACAA253.407448E-546.032
TCCGGAC206.299485E-446.043
GTCATTG253.407448E-546.043
CTTCGTA253.407448E-546.041
TCGCCCC206.299485E-446.014
GTTGACG301.8540086E-646.015
CATGGTT253.407448E-546.030
CAGGTAG253.407448E-546.039
AGCAATT253.407448E-546.029
TGATATT301.8540086E-646.034
TTAGGCT501.6370905E-1146.020
TTAGGCA253.407448E-546.037
AGGTAGT551.8189894E-1246.010
TAATAGC206.299485E-446.038
CCTTATG206.299485E-446.030
AATCCTC253.407448E-546.016
CGGAACT206.299485E-446.044
Produced by FastQC (version 0.11.5)