FastQCFastQC Report
Fri 17 Jun 2016
SRR1527726_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527726_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1207397
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC87930.7282608785676956No Hit
ACCTGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT57450.47581698480284446No Hit
ACCTGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC56430.46736905922409944No Hit
ACCTGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG44610.36947250987040714No Hit
ACCTGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC36280.30048111764398955No Hit
ACCTGAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG32840.271990074515673No Hit
ACCTGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT31360.2597323001465135No Hit
ACCTGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA27960.23157254821736345No Hit
ACCTGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG26020.21550492505778962No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC25420.2105355570702925No Hit
ACCTGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC21160.17525304435906333No Hit
ACCTGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT20110.16655665038094347No Hit
ACCTGAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG19180.158854130000323No Hit
ACCTGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC16380.13566374605867002No Hit
ACCTGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG15410.12762993447888307No Hit
ACCTGAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG15290.12663606088138368No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT13260.10982303252368526No Hit
ACCTGAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTC12990.10758681692931157No Hit
ACCTGAGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT12700.10518495573535465No Hit
ACCTGAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT12660.10485366453618819No Hit
ACCTGAGGGATCTTGGATCCTTTTGAAAAGTTAAGAATATATGAAGGTAA12490.10344567693973067No Hit
ACCTGAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTG12390.1026174489418145No Hit
ACCTGAGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT12180.10087817014619054No Hit
ACCTGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG12160.10071252454660728No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTA207.856639E-444.00000437
ACGTTCA207.856639E-444.00000430
GACGTAT207.856639E-444.00000435
CGCATAT207.856639E-444.00000437
ACCGATA207.856639E-444.00000424
GCGTTTA207.856639E-444.00000417
TCGATTG207.856639E-444.00000411
CGTTAAC207.856639E-444.00000435
TCAATCG207.856639E-444.00000416
AATTGCG207.856639E-444.00000441
TCGTAAC207.856639E-444.00000425
TTACGAA207.856639E-444.00000441
ACGTATT207.856639E-444.00000436
TCATAGG207.856639E-444.00000444
CGGTATG207.856639E-444.00000415
ACGTTTA351.4461693E-744.012
CCGATAC502.7284841E-1144.014
CGATTGC302.527824E-644.044
CGTTATG254.4429733E-544.036
CGAATCT351.4461693E-744.018
Produced by FastQC (version 0.11.5)