##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527726_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1207397
Sequences flagged as poor quality	0
Sequence length	50
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.847415555943904	33.0	31.0	34.0	3.0	34.0
2	30.409839514260845	34.0	31.0	34.0	16.0	34.0
3	32.441226870697875	34.0	31.0	34.0	28.0	34.0
4	36.29064425371274	37.0	35.0	37.0	35.0	37.0
5	36.31747055856525	37.0	37.0	37.0	35.0	37.0
6	36.08714449348474	37.0	35.0	37.0	35.0	37.0
7	36.56654687729057	37.0	37.0	37.0	35.0	37.0
8	36.707343980480324	37.0	37.0	37.0	35.0	37.0
9	38.776800836841566	39.0	39.0	39.0	39.0	39.0
10	38.31029727587529	39.0	39.0	39.0	37.0	39.0
11	38.079644888963614	39.0	38.0	39.0	37.0	39.0
12	37.67764455270304	39.0	37.0	39.0	35.0	39.0
13	37.5592601273649	39.0	37.0	39.0	35.0	39.0
14	38.34167386534835	40.0	38.0	40.0	35.0	40.0
15	38.391095886440006	40.0	38.0	40.0	35.0	40.0
16	38.45618798125223	40.0	38.0	40.0	35.0	40.0
17	38.39081594537671	40.0	38.0	40.0	35.0	40.0
18	38.33668213520491	40.0	38.0	40.0	35.0	40.0
19	38.278771605362614	40.0	38.0	40.0	34.0	40.0
20	38.19060010916045	40.0	38.0	40.0	34.0	40.0
21	38.18389891642931	40.0	38.0	40.0	34.0	40.0
22	38.22821491191381	40.0	38.0	40.0	34.0	40.0
23	38.16717782137938	40.0	38.0	40.0	35.0	40.0
24	38.09994392896454	40.0	38.0	40.0	34.0	40.0
25	38.047119547257445	40.0	38.0	40.0	34.0	40.0
26	37.967020789350975	40.0	38.0	40.0	34.0	40.0
27	37.847332733144114	40.0	37.0	40.0	34.0	40.0
28	37.71334035118524	40.0	37.0	40.0	34.0	40.0
29	37.72256349817003	40.0	37.0	40.0	34.0	40.0
30	37.869482034492385	40.0	37.0	40.0	34.0	40.0
31	37.935751869517645	40.0	37.0	40.0	34.0	40.0
32	37.909617135043405	40.0	37.0	40.0	34.0	40.0
33	37.798838327410124	40.0	37.0	40.0	34.0	40.0
34	37.477414636610824	40.0	36.0	40.0	33.0	40.0
35	37.45482471796766	40.0	36.0	40.0	33.0	40.0
36	37.417725901257	40.0	36.0	40.0	33.0	40.0
37	37.33001241513769	40.0	35.0	40.0	33.0	40.0
38	37.27960563095651	40.0	35.0	40.0	33.0	40.0
39	37.19482490017782	40.0	35.0	40.0	33.0	40.0
40	36.981261341547146	40.0	35.0	40.0	33.0	40.0
41	36.8396260716235	39.0	35.0	40.0	33.0	40.0
42	36.75089055215476	39.0	35.0	40.0	32.0	40.0
43	36.62022681851951	39.0	35.0	40.0	32.0	40.0
44	36.256545278810535	39.0	35.0	40.0	31.0	40.0
45	36.25543959443331	39.0	35.0	40.0	31.0	40.0
46	36.05044322621308	38.0	35.0	40.0	31.0	40.0
47	36.012617225320255	38.0	35.0	40.0	31.0	40.0
48	35.92551331500741	37.0	35.0	40.0	31.0	40.0
49	35.826839059563675	37.0	35.0	40.0	31.0	40.0
50	35.299385372002746	37.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	3.0
15	11.0
16	20.0
17	39.0
18	97.0
19	186.0
20	358.0
21	612.0
22	1055.0
23	1637.0
24	2354.0
25	3283.0
26	4488.0
27	5784.0
28	7994.0
29	10631.0
30	14652.0
31	19604.0
32	25732.0
33	39306.0
34	68513.0
35	91180.0
36	112705.0
37	227706.0
38	348245.0
39	221200.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.39464815632306	0.41759255654933713	0.13582939165825325	0.051929895469344386
8	99.33584396847101	0.5150749919040712	0.10212051214306479	0.046960527481847314
9	98.40657215480907	0.879246842587815	0.2555911601569327	0.45858984244618795
10	58.62810657969169	30.85439172037035	4.130455848407773	6.387045851530193
11	34.7637106933345	24.69163001067586	22.80153089663135	17.743128399358287
12	31.897544883745777	16.649370505310184	26.960312142567854	24.492772468376184
13	24.67009608273004	17.45507070168304	29.85438923568636	28.020443979900563
14	25.7158995756988	18.535825416163863	30.025252671656467	25.723022336480877
15	26.055721523243804	19.550653182010556	27.464123233700267	26.929502061045373
16	30.762624058201236	19.413995562354387	24.30145180085755	25.521928578586827
17	31.310248410423412	19.937601302636995	22.626940434670615	26.12520985226897
18	29.765271903110573	19.912091880301176	25.010829081072757	25.31180713551549
19	30.445329912199547	20.928907393342868	22.662471415781223	25.96329127867636
20	29.08463413442306	22.75962255993679	22.686158736521627	25.469584569118524
21	31.07809610260751	20.617990602925136	24.48018340280786	23.823729891659497
22	30.25748780227216	20.052890639946927	23.829858778844077	25.859762778936833
23	30.072875781536645	20.55844100987496	24.42808786173893	24.940595346849463
24	29.83972960012324	21.36935904263469	23.157006353336975	25.633905003905095
25	28.06483700058887	22.484982155827783	22.76417781392533	26.686003029658018
26	27.984995821589752	21.76508638003904	22.73046893441014	27.519448863961067
27	27.053156501134257	20.042040853174225	24.10209732175912	28.802705323932393
28	28.88817845331734	22.333830546208084	22.446138262725515	26.331852737749056
29	30.731482685479588	21.626441013187875	22.31213097266268	25.329945328669858
30	29.15345988104989	20.80500448485461	22.507592780170896	27.533942853924597
31	31.315466246810285	21.206777886643746	22.423693283981986	25.05406258256398
32	32.508280209409165	21.28479696404745	22.705042334874115	23.50188049166927
33	32.02293860263028	20.95557633487577	22.466926785473213	24.554558277020732
34	29.182613506576544	23.207942375208816	24.619160060858196	22.990284057356444
35	28.123392720041547	24.676639083913578	25.422789687236264	21.77717850880862
36	30.05424065158353	24.246954398594664	22.2872013099254	23.411603639896406
37	29.174414049397175	26.11692757228981	20.93727249612182	23.771385882191193
38	30.235125646328427	24.68608088308982	21.391638375778637	23.687155094803117
39	30.422719287856438	22.750760520359087	21.47727715076317	25.349243041021303
40	28.311731766767682	23.491030704896566	22.146982309878194	26.050255218457558
41	26.29657022503783	23.930736948990265	22.53956238089046	27.233130445081443
42	25.810980149859574	21.58179952410019	23.529212015600503	29.078008310439728
43	25.959895543884905	21.17886660311397	25.250104149670737	27.61113370333039
44	26.915173716681423	20.985475365600546	24.15411004002826	27.945240877689777
45	27.396622651870096	21.42054353290591	23.94374012855755	27.239093686666436
46	27.22029291111374	23.764097475809532	23.051738574801824	25.9638710382749
47	26.79317573258837	22.53500712690192	24.117916476519323	26.553900663990387
48	28.155776434760067	21.76392686084196	24.779505001254766	25.30079170314321
49	28.223773953388985	21.452430310825683	24.169349435189915	26.154446300595414
50	25.70331051013047	23.15452166934322	24.91475463331448	26.22741318721183
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	4.5
6	9.0
7	8.0
8	7.0
9	7.0
10	7.0
11	204.0
12	401.0
13	1143.0
14	1885.0
15	2323.0
16	2761.0
17	2483.0
18	2205.0
19	2191.0
20	2177.0
21	2136.0
22	2095.0
23	2447.5
24	2800.0
25	3106.0
26	3412.0
27	3881.5
28	4351.0
29	5289.0
30	6227.0
31	7255.5
32	8284.0
33	10323.0
34	12362.0
35	15429.5
36	18497.0
37	19179.0
38	19861.0
39	22190.0
40	24519.0
41	25610.0
42	26701.0
43	29271.0
44	31841.0
45	34949.5
46	38058.0
47	41861.5
48	45665.0
49	48343.0
50	51021.0
51	53985.5
52	56950.0
53	62498.5
54	68047.0
55	72508.0
56	76969.0
57	90487.5
58	104006.0
59	107944.5
60	111883.0
61	114025.5
62	116168.0
63	113077.5
64	109987.0
65	98605.0
66	87223.0
67	72482.0
68	57741.0
69	47811.0
70	37881.0
71	32464.5
72	27048.0
73	22443.0
74	17838.0
75	18491.5
76	19145.0
77	13437.5
78	7730.0
79	4875.0
80	2020.0
81	1556.0
82	1092.0
83	743.5
84	395.0
85	247.5
86	100.0
87	59.5
88	19.0
89	11.5
90	4.0
91	3.5
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1207397.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.779406051617569
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.01863135584044	5.883837776965925
2	9.651543844710288	1.6946964487540357
3	3.8184602406177484	1.0057143883302164
4	1.772104289250161	0.6223209248456126
5	0.9385279420251611	0.41198589469139413
6	0.6802415509318672	0.3583270073287773
7	0.43898749140288623	0.26978346070248405
8	0.36046170503526903	0.25317117396504224
9	0.32640233085209647	0.25790567388704777
>10	8.225338736433324	21.304836011363133
>50	4.674649033437133	28.50817055360585
>100	2.006664764201611	29.74711452501057
>500	0.0529812478997125	3.2215367344416754
>1k	0.03216718622482544	4.784337138167862
>5k	0.002838281137484598	1.6762622879403752
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	8793	0.7282608785676956	No Hit
ACCTGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	5745	0.47581698480284446	No Hit
ACCTGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	5643	0.46736905922409944	No Hit
ACCTGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	4461	0.36947250987040714	No Hit
ACCTGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	3628	0.30048111764398955	No Hit
ACCTGAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	3284	0.271990074515673	No Hit
ACCTGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	3136	0.2597323001465135	No Hit
ACCTGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	2796	0.23157254821736345	No Hit
ACCTGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	2602	0.21550492505778962	No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC	2542	0.2105355570702925	No Hit
ACCTGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	2116	0.17525304435906333	No Hit
ACCTGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2011	0.16655665038094347	No Hit
ACCTGAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	1918	0.158854130000323	No Hit
ACCTGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	1638	0.13566374605867002	No Hit
ACCTGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1541	0.12762993447888307	No Hit
ACCTGAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG	1529	0.12663606088138368	No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	1326	0.10982303252368526	No Hit
ACCTGAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTC	1299	0.10758681692931157	No Hit
ACCTGAGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	1270	0.10518495573535465	No Hit
ACCTGAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT	1266	0.10485366453618819	No Hit
ACCTGAGGGATCTTGGATCCTTTTGAAAAGTTAAGAATATATGAAGGTAA	1249	0.10344567693973067	No Hit
ACCTGAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1239	0.1026174489418145	No Hit
ACCTGAGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	1218	0.10087817014619054	No Hit
ACCTGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1216	0.10071252454660728	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.013251647966658854	0.0	0.0	0.0	0.0
7	0.013500116366033708	0.0	0.0	0.0	0.0
8	0.020540054348321225	0.0	0.0	0.0	0.0
9	0.03785001950476935	0.0	0.0	0.0	0.0
10	0.056153858258716896	0.0	0.0	0.0	0.0
11	0.08646700298244903	0.0	0.0	0.0	0.0
12	0.09806219495327552	0.0	0.0	0.0	0.0
13	0.10874633612639421	0.0	0.0	0.0	0.0
14	0.12415137688763514	0.0	0.0	0.0	0.0
15	0.13524963205971194	0.0	0.0	0.0	0.0
16	0.14792152042782947	0.0	0.0	0.0	0.0
17	0.15943388959886434	0.0	0.0	0.0	0.0
18	0.17119472716927406	0.0	0.0	0.0	0.0
19	0.18270709634030893	0.0	0.0	0.0	0.0
20	0.19305994631426118	0.0	0.0	0.0	0.0
21	0.20275021388988046	0.0	0.0	0.0	0.0
22	0.21285459546445784	0.0	0.0	0.0	0.0
23	0.22511236983361727	0.0	0.0	0.0	0.0
24	0.235796511006736	0.0	0.0	0.0	0.0
25	0.24656347497964629	0.0	0.0	0.0	0.0
26	0.2606433509442213	0.0	0.0	0.0	0.0
27	0.2743091129098383	0.0	0.0	0.0	0.0
28	0.2929442428629523	0.0	0.0	0.0	0.0
29	0.30304862443752967	0.0	0.0	0.0	0.0
30	0.31845366519877055	0.0	0.0	0.0	0.0
31	0.329634743170639	0.0	0.0	0.0	0.0
32	0.34288639113729785	0.0	0.0	0.0	0.0
33	0.35555827950541535	0.0	0.0	0.0	0.0
34	0.3707148518672814	0.0	0.0	0.0	0.0
35	0.3908407922166446	0.0	0.0	0.0	0.0
36	0.4055004277797609	0.0	0.0	0.0	0.0
37	0.4194146581447527	0.0	0.0	0.0	0.0
38	0.4313411413147457	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTA	20	7.856639E-4	44.000004	37
ACGTTCA	20	7.856639E-4	44.000004	30
GACGTAT	20	7.856639E-4	44.000004	35
CGCATAT	20	7.856639E-4	44.000004	37
ACCGATA	20	7.856639E-4	44.000004	24
GCGTTTA	20	7.856639E-4	44.000004	17
TCGATTG	20	7.856639E-4	44.000004	11
CGTTAAC	20	7.856639E-4	44.000004	35
TCAATCG	20	7.856639E-4	44.000004	16
AATTGCG	20	7.856639E-4	44.000004	41
TCGTAAC	20	7.856639E-4	44.000004	25
TTACGAA	20	7.856639E-4	44.000004	41
ACGTATT	20	7.856639E-4	44.000004	36
TCATAGG	20	7.856639E-4	44.000004	44
CGGTATG	20	7.856639E-4	44.000004	15
ACGTTTA	35	1.4461693E-7	44.0	12
CCGATAC	50	2.7284841E-11	44.0	14
CGATTGC	30	2.527824E-6	44.0	44
CGTTATG	25	4.4429733E-5	44.0	36
CGAATCT	35	1.4461693E-7	44.0	18
>>END_MODULE