FastQCFastQC Report
Fri 17 Jun 2016
SRR1527725_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527725_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1528645
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC114070.7462164204246244No Hit
ACCTGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG74370.48650929417883154No Hit
ACCTGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT67080.4388200007195915No Hit
ACCTGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA46160.30196677449636766No Hit
ACCTGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT45070.2948362765717351No Hit
ACCTGAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC44270.2896028835995277No Hit
ACCTGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC41220.26965057289298694No Hit
ACCTGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC36470.2385773021205054No Hit
ACCTGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG34380.22490506298061355No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC31970.20913946665183872No Hit
ACCTGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT28070.18362667591232756No Hit
ACCTGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC26700.17466449044742238No Hit
ACCTGAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG25250.16517896568529644No Hit
ACCTGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC21680.14182494954682087No Hit
ACCTGAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT19680.12874146711630235No Hit
ACCTGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC18900.12363890896840012No Hit
ACCTGAGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC16620.10872373899760898No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC16550.10826581711254084No Hit
ACCTGAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG16540.10820039970038825No Hit
ACCTGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG16480.10780789522747271No Hit
ACCTGAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG15940.10427535497123269No Hit
ACCTGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC15600.10205116295804455No Hit
ACCTGAGGGATCTTGGATCCTTTTGAAAAGTTAAGAATATATGAAGGTAAAT15440.10100448436360307No Hit
ACCTGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT15360.10048114506638231No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGC206.3121103E-446.00000420
ACGATTC206.3121103E-446.00000442
GTTACGA206.3121103E-446.00000428
AACGAAC206.3121103E-446.00000444
AGTCGTA405.6134013E-946.00000441
GTACGGT206.3121103E-446.00000431
CGTATCA253.4176883E-546.031
GTTACGT253.4176883E-546.046
TACGATA253.4176883E-546.041
GTGTCGA253.4176883E-546.041
TTACGTG253.4176883E-546.029
ATAACCG253.4176883E-546.037
CGATCTA301.8618102E-646.042
GCGCGTA351.019871E-745.99999637
ACCTGAG1613250.043.3838541
CCTGAGG1608700.043.294962
CTGAGGG1587050.043.292843
AGGGATT50650.043.2754176
TGAGGGA490350.043.1903764
TGAGGGG938200.043.114584
Produced by FastQC (version 0.11.5)