##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527725_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1528645
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.062772586179264	33.0	31.0	34.0	31.0	34.0
2	32.66267249753867	34.0	31.0	34.0	31.0	34.0
3	33.03491000199523	34.0	31.0	34.0	31.0	34.0
4	36.4449528831089	37.0	37.0	37.0	35.0	37.0
5	36.237427918188985	37.0	37.0	37.0	35.0	37.0
6	35.878960778990546	37.0	35.0	37.0	35.0	37.0
7	36.49331335921682	37.0	37.0	37.0	35.0	37.0
8	36.63775108020502	37.0	37.0	37.0	35.0	37.0
9	38.70813629063648	39.0	39.0	39.0	39.0	39.0
10	38.056338129519936	39.0	38.0	39.0	37.0	39.0
11	37.70640796260741	39.0	38.0	39.0	35.0	39.0
12	37.30476271469177	39.0	37.0	39.0	35.0	39.0
13	37.173460810063816	39.0	37.0	39.0	34.0	39.0
14	37.96760464332792	40.0	38.0	40.0	34.0	40.0
15	38.034804025787544	40.0	38.0	40.0	34.0	40.0
16	38.110817096186494	40.0	38.0	40.0	34.0	40.0
17	38.04042011062084	40.0	38.0	40.0	34.0	40.0
18	38.045537714773545	40.0	38.0	40.0	34.0	40.0
19	37.99891341678415	40.0	38.0	40.0	34.0	40.0
20	37.974846350853205	40.0	38.0	40.0	34.0	40.0
21	37.957796610723875	40.0	38.0	40.0	34.0	40.0
22	37.96342316234312	40.0	38.0	40.0	34.0	40.0
23	37.93680874238296	40.0	37.0	40.0	34.0	40.0
24	37.86513088388737	40.0	37.0	40.0	34.0	40.0
25	37.802842386558034	40.0	37.0	40.0	34.0	40.0
26	37.70372519453503	40.0	37.0	40.0	34.0	40.0
27	37.607072276427814	40.0	37.0	40.0	33.0	40.0
28	37.510501784259915	40.0	36.0	40.0	33.0	40.0
29	37.43699223822405	40.0	36.0	40.0	33.0	40.0
30	37.294132385216976	40.0	36.0	40.0	33.0	40.0
31	37.22109449872273	40.0	36.0	40.0	33.0	40.0
32	37.10244890082393	39.0	35.0	40.0	32.0	40.0
33	36.9596845572386	39.0	35.0	40.0	32.0	40.0
34	36.78903865841971	39.0	35.0	40.0	31.0	40.0
35	36.6728815388792	39.0	35.0	40.0	31.0	40.0
36	36.65051401731599	39.0	35.0	40.0	31.0	40.0
37	36.54156197154997	39.0	35.0	40.0	31.0	40.0
38	36.4959686519761	39.0	35.0	40.0	31.0	40.0
39	36.40913554160711	38.0	35.0	40.0	31.0	40.0
40	36.158790301214474	38.0	35.0	40.0	30.0	40.0
41	36.018353509153535	38.0	35.0	40.0	30.0	40.0
42	35.932098688707974	38.0	35.0	40.0	30.0	40.0
43	35.788168606838084	38.0	35.0	40.0	30.0	40.0
44	35.63979668268303	38.0	34.0	40.0	30.0	40.0
45	35.53195019118239	37.0	34.0	40.0	29.0	40.0
46	35.36786696715065	37.0	34.0	40.0	29.0	40.0
47	35.19890229582408	37.0	34.0	40.0	29.0	40.0
48	34.98804823879972	36.0	34.0	40.0	28.0	40.0
49	35.2836577491831	37.0	34.0	40.0	29.0	40.0
50	35.32834176672805	36.0	34.0	40.0	29.0	40.0
51	35.31813403373576	36.0	34.0	40.0	30.0	40.0
52	34.818065018365935	35.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	2.0
14	2.0
15	6.0
16	18.0
17	70.0
18	157.0
19	337.0
20	658.0
21	1154.0
22	1896.0
23	2965.0
24	4346.0
25	6190.0
26	8123.0
27	11139.0
28	14697.0
29	18981.0
30	23967.0
31	30844.0
32	41195.0
33	56939.0
34	102950.0
35	119440.0
36	155773.0
37	244392.0
38	470977.0
39	211425.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.4939963169997	0.39512116940165964	0.07712712892790674	0.033755384670737805
8	99.41569167465303	0.48683638123959455	0.06077277588975858	0.03669916821760448
9	98.47786765403347	0.841333337694494	0.2465582264031217	0.43424078186891
10	58.76570426750488	30.700784027684648	4.118287764654318	6.415223940156152
11	34.739458801749265	24.746425756143513	22.68636603004622	17.82774941206101
12	32.01083312345247	16.712251700035	26.88479012458746	24.39212505192507
13	24.712735788884928	17.36354745542621	29.823601948130534	28.10011480755833
14	25.898295549326367	18.41199231999581	29.90498120884836	25.784730921829464
15	26.083034321245286	19.538283905027	27.405708977558557	26.97297279616916
16	30.96493953795682	19.35132094109489	24.2516084506213	25.432131070326992
17	31.454392615682515	19.87910862234201	22.53230802442686	26.134190737548614
18	29.86612326602972	19.912406085127678	24.939668791642273	25.281801857200332
19	30.49792463259946	20.81346552011749	22.717112213757936	25.971497633525114
20	29.13285949321131	22.7313732096072	22.595762914214877	25.540004382966615
21	31.070588658583254	20.56442143205257	24.477756444432817	23.88723346493136
22	30.37075318337482	20.047820128283544	23.78956526858754	25.7918614197541
23	30.104438898501613	20.461977764621608	24.431833421101697	25.001749915775086
24	29.76269833741647	21.314104975321282	23.172940741637202	25.750255945625046
25	28.08899384749239	22.386296360502275	22.851283326082903	26.673426465922432
26	28.076564539183398	21.67134946308659	22.701673704489924	27.55041229324009
27	27.112704388527092	19.987047352393787	24.006227737636927	28.894020521442194
28	28.917243702756362	22.292029869590387	22.35770895139159	26.433017476261654
29	30.745398702772718	21.58002675572157	22.29811368892058	25.37646085258513
30	29.225359713995076	20.731039580805223	22.519486211644953	27.52411449355475
31	31.3972832148733	21.152458549892224	22.438434037987893	25.01182419724658
32	32.71328529514701	21.147094322095715	22.65333023690916	23.48629014584812
33	32.13833165973787	20.785270615479725	22.43830320316359	24.638094521618818
34	29.196118130762866	23.029153269725803	24.662887720824653	23.111840878686678
35	28.13707564542454	24.674597437599964	25.357620637885187	21.830706279090307
36	30.150558174069193	24.17029460731563	22.301319142116057	23.37782807649912
37	29.398257934314376	25.99602916308234	20.90426488818529	23.701448014418
38	30.318942592949966	24.634496563950425	21.340926114303844	23.70563472879576
39	30.492756657039404	22.65941405623935	21.50937595059677	25.338453336124477
40	28.325216122775398	23.373183440236286	22.202735102002098	26.098865334986215
41	26.370478430243775	23.851711810132503	22.48756251451449	27.290247245109228
42	25.854662135420586	21.46162123972538	23.461104442169372	29.222612182684664
43	25.945657755724845	21.11373144189789	25.199703004948827	27.740907797428445
44	26.74276892280418	20.854743907185775	24.2300861220231	28.172401047986945
45	27.493957066552404	21.3549254405045	23.81861059958329	27.332506893359803
46	27.018634149851668	23.68280405195451	23.035433341292453	26.263128456901374
47	26.85633355030108	22.42907934805007	24.054374952981235	26.66021214866761
48	28.308338430440028	21.588203932240642	24.726080940964057	25.37737669635527
49	28.248939420205478	21.387045389871425	24.197769920419717	26.166245269503385
50	25.828756840208158	23.010247637613706	24.908464686045484	26.252530836132653
51	26.054316077310297	22.547877368519178	24.539183394444102	26.858623159726424
52	26.402860049259314	22.33690621432707	25.151490372192363	26.108743364221255
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	3.0
6	6.0
7	8.5
8	11.0
9	9.5
10	8.0
11	221.0
12	434.0
13	1324.0
14	2699.0
15	3184.0
16	2911.0
17	2638.0
18	2580.5
19	2523.0
20	2377.0
21	2231.0
22	2745.0
23	3259.0
24	3437.5
25	3616.0
26	3601.0
27	3586.0
28	5474.5
29	7363.0
30	7541.5
31	7720.0
32	10372.0
33	13024.0
34	14449.0
35	15874.0
36	19006.0
37	22138.0
38	24467.5
39	28775.5
40	30754.0
41	32440.5
42	34127.0
43	36990.0
44	39853.0
45	43630.0
46	47407.0
47	51613.5
48	55820.0
49	58371.0
50	60922.0
51	65526.5
52	70131.0
53	78223.0
54	86315.0
55	87968.5
56	89622.0
57	102766.0
58	115910.0
59	131281.0
60	146652.0
61	142183.5
62	137715.0
63	138467.0
64	123431.0
65	107643.0
66	97227.0
67	86811.0
68	69192.0
69	51573.0
70	45003.5
71	38434.0
72	33033.5
73	27633.0
74	27111.5
75	26590.0
76	19324.5
77	12059.0
78	7984.0
79	3909.0
80	2825.5
81	1742.0
82	1304.5
83	867.0
84	564.5
85	262.0
86	143.5
87	25.0
88	20.5
89	10.0
90	4.0
91	3.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1528645.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.653163349599934
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.44179108592817	5.084898804206011
2	9.778387495398128	1.496711935959345
3	4.240459405766531	0.9735878552903621
4	2.2893062686310484	0.7008173932438608
5	1.2186161855447915	0.46631343642203354
6	0.7194679577091623	0.3303723483090765
7	0.5269463572421503	0.2822964583915573
8	0.372963146827386	0.22834783088406474
9	0.2976852780752207	0.205041065389267
>10	6.050362890600907	13.56837862134711
>50	5.063211885166934	27.743696560443258
>100	2.8810436947528695	37.50114256219241
>500	0.07356584256197946	3.676791840023065
>1k	0.043626255472801766	6.068804735537243
>5k	0.0017108335479530104	0.9260228366918836
>10k+	8.554167739765052E-4	0.7467757156694461
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	11407	0.7462164204246244	No Hit
ACCTGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	7437	0.48650929417883154	No Hit
ACCTGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	6708	0.4388200007195915	No Hit
ACCTGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	4616	0.30196677449636766	No Hit
ACCTGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	4507	0.2948362765717351	No Hit
ACCTGAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	4427	0.2896028835995277	No Hit
ACCTGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	4122	0.26965057289298694	No Hit
ACCTGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3647	0.2385773021205054	No Hit
ACCTGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	3438	0.22490506298061355	No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC	3197	0.20913946665183872	No Hit
ACCTGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	2807	0.18362667591232756	No Hit
ACCTGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2670	0.17466449044742238	No Hit
ACCTGAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	2525	0.16517896568529644	No Hit
ACCTGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	2168	0.14182494954682087	No Hit
ACCTGAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	1968	0.12874146711630235	No Hit
ACCTGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1890	0.12363890896840012	No Hit
ACCTGAGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	1662	0.10872373899760898	No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	1655	0.10826581711254084	No Hit
ACCTGAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG	1654	0.10820039970038825	No Hit
ACCTGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1648	0.10780789522747271	No Hit
ACCTGAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	1594	0.10427535497123269	No Hit
ACCTGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	1560	0.10205116295804455	No Hit
ACCTGAGGGATCTTGGATCCTTTTGAAAAGTTAAGAATATATGAAGGTAAAT	1544	0.10100448436360307	No Hit
ACCTGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1536	0.10048114506638231	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.011644299363161493	0.0	0.0	0.0	0.0
7	0.011709716775314086	0.0	0.0	0.0	0.0
8	0.016943109747521497	0.0	0.0	0.0	0.0
9	0.032904958312754105	0.0	0.0	0.0	0.0
10	0.05043682476964894	0.0	0.0	0.0	0.0
11	0.07810839011019563	0.0	0.0	0.0	0.0
12	0.08693974075079564	0.0	0.0	0.0	0.0
13	0.09511691726986972	0.0	0.0	0.0	0.0
14	0.10813498228823566	0.0	0.0	0.0	0.0
15	0.11768592446251419	0.0	0.0	0.0	0.0
16	0.12749853628540309	0.0	0.0	0.0	0.0
17	0.13776906999336014	0.0	0.0	0.0	0.0
18	0.14640416839750237	0.0	0.0	0.0	0.0
19	0.15909514635510533	0.0	0.0	0.0	0.0
20	0.16936568006306238	0.0	0.0	0.0	0.0
21	0.1797670485953246	0.0	0.0	0.0	0.0
22	0.18997216489112906	0.0	0.0	0.0	0.0
23	0.2012893771935276	0.0	0.0	0.0	0.0
24	0.21175616313794243	0.0	0.0	0.0	0.0
25	0.22052209636638984	0.0	0.0	0.0	0.0
26	0.233343909148298	0.0	0.0	0.0	0.0
27	0.24564238263298543	0.0	0.0	0.0	0.0
28	0.26323966650203284	0.0	0.0	0.0	0.0
29	0.2735756176221425	0.0	0.0	0.0	0.0
30	0.28580867369467733	0.0	0.0	0.0	0.0
31	0.2968642163484655	0.0	0.0	0.0	0.0
32	0.31197563855571436	0.0	0.0	0.0	0.0
33	0.32368535533102843	0.0	0.0	0.0	0.0
34	0.33513340245773215	0.0	0.0	0.0	0.0
35	0.35488946092781515	0.0	0.0	0.0	0.0
36	0.36993546572291147	0.0	0.0	0.0	0.0
37	0.3820376869711411	0.0	0.0	0.0	0.0
38	0.3933548992735396	0.0	0.0	0.0	0.0
39	0.40578420758253225	0.0	0.0	0.0	0.0
40	0.4185406029522878	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGC	20	6.3121103E-4	46.000004	20
ACGATTC	20	6.3121103E-4	46.000004	42
GTTACGA	20	6.3121103E-4	46.000004	28
AACGAAC	20	6.3121103E-4	46.000004	44
AGTCGTA	40	5.6134013E-9	46.000004	41
GTACGGT	20	6.3121103E-4	46.000004	31
CGTATCA	25	3.4176883E-5	46.0	31
GTTACGT	25	3.4176883E-5	46.0	46
TACGATA	25	3.4176883E-5	46.0	41
GTGTCGA	25	3.4176883E-5	46.0	41
TTACGTG	25	3.4176883E-5	46.0	29
ATAACCG	25	3.4176883E-5	46.0	37
CGATCTA	30	1.8618102E-6	46.0	42
GCGCGTA	35	1.019871E-7	45.999996	37
ACCTGAG	161325	0.0	43.383854	1
CCTGAGG	160870	0.0	43.29496	2
CTGAGGG	158705	0.0	43.29284	3
AGGGATT	5065	0.0	43.275417	6
TGAGGGA	49035	0.0	43.190376	4
TGAGGGG	93820	0.0	43.11458	4
>>END_MODULE