FastQCFastQC Report
Fri 17 Jun 2016
SRR1527724_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527724_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1540596
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC107110.6952504095817462No Hit
ACCTGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG71030.46105533183261543No Hit
ACCTGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT66240.42996346868354846No Hit
ACCTGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT44660.2898878096528876No Hit
ACCTGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA43300.28106005727653455No Hit
ACCTGAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC40490.2628203630283345No Hit
ACCTGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC39560.25678373824156364No Hit
ACCTGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC35460.23017066122461696No Hit
ACCTGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG32850.21322916585529236No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC29550.19180888435384746No Hit
ACCTGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC26120.16954477358113354No Hit
ACCTGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT25150.16324850901858762No Hit
ACCTGAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG22430.1455930042658815No Hit
ACCTGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC20710.13442849390755265No Hit
ACCTGAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT18650.12105704545513556No Hit
ACCTGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC17020.11047672459230064No Hit
ACCTGAGGGATCTTGGATCCTTTTGAAAAGTTAAGAATATATGAAGGTAAAT15900.10320681087059815No Hit
ACCTGAGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC15790.10249280148721662No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC15460.10035077333707214No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTTA206.3121266E-446.029
CGAACTA206.3121266E-446.031
CGACGCA206.3121266E-446.016
TCGATTG253.4177014E-546.011
GCCGATA206.3121266E-446.034
CTAACGC253.4177014E-546.029
TCAATCG301.8618193E-646.016
TACGATA206.3121266E-446.041
ATATACG206.3121266E-446.023
ATCGATT253.4177014E-546.043
TACGTAC253.4177014E-546.025
ATACGTA351.019871E-745.99999646
ATACCGA453.110472E-1045.99999643
AGGGATT47250.043.9555556
ACCTGAG1618100.043.5181961
CCTGAGG1612750.043.4343832
CTGAGGG1589800.043.4002383
TGAGGGA490100.043.334424
TGAGGGG937250.043.2760734
GAGGGAT159200.042.9805265
Produced by FastQC (version 0.11.5)