FastQCFastQC Report
Fri 17 Jun 2016
SRR1527723_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527723_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2024702
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC274141.3539770297061No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG156970.7752745836177373No Hit
TGCGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC89370.44139828972362355No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT73260.3618310250101002No Hit
TGCGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT62320.3077983821816741No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTG54880.2710522338596001No Hit
TGCGGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC54030.2668540851937717No Hit
TGCGGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG51280.25327183951020943No Hit
TGCGGAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG38170.18852157008784504No Hit
TGCGGAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC31390.15503516072982593No Hit
TGCGGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT30040.14836751284880442No Hit
TGCGGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT28710.14179864493639063No Hit
TGCGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGC27320.1349334371181537No Hit
TGCGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACT26650.13162430816979487No Hit
TGCGGAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA26360.13019199862498285No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC25320.1250554402573811No Hit
TGCGGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG24690.12194387124623773No Hit
TGCGGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT23730.11720243275306687No Hit
TGCGGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG23060.11389330380470804No Hit
TGCGGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC20280.10016288816823414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAA302.5286772E-644.011
ATAACGC207.857904E-444.012
TCGACGT207.857904E-444.015
TCGAACG207.857904E-444.011
CGGAGGG2065050.042.0365643
TGCGGAG2108700.042.026081
GCGGAGG2102050.041.9528582
GAGGGAT224900.041.789245
GAGGGGA311900.041.6370665
AGGGATT77250.041.607776
GGAGGGG1192750.041.360554
GGAGGGA682300.041.2786184
GAGGGGT220050.041.0306785
AGGGGGG247700.040.740416
GAGGGGG487000.040.720335
CTATCGA650.040.61538738
AGGGATA22300.040.5470856
AGGGATC67950.040.4385576
AGGGGAG141950.040.388876
AGGGATG62700.040.3859636
Produced by FastQC (version 0.11.5)