##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527723_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2024702
Sequences flagged as poor quality	0
Sequence length	50
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13059255139769	31.0	31.0	34.0	31.0	34.0
2	32.74562034314185	34.0	31.0	34.0	31.0	34.0
3	33.03508269365072	34.0	31.0	34.0	31.0	34.0
4	36.42770541047522	37.0	37.0	37.0	35.0	37.0
5	36.44620047789749	37.0	37.0	37.0	35.0	37.0
6	35.27242231202419	37.0	35.0	37.0	32.0	37.0
7	36.24054700395416	37.0	35.0	37.0	35.0	37.0
8	36.56594106194393	37.0	37.0	37.0	35.0	37.0
9	38.69750264483366	39.0	39.0	39.0	39.0	39.0
10	37.91091627311081	39.0	38.0	39.0	35.0	39.0
11	37.464337467933554	39.0	37.0	39.0	35.0	39.0
12	36.86888638426791	38.0	35.0	39.0	34.0	39.0
13	36.700110929904746	38.0	35.0	39.0	33.0	39.0
14	37.247972788094245	39.0	36.0	40.0	33.0	40.0
15	37.25197930362098	39.0	36.0	40.0	33.0	40.0
16	37.33094499832568	39.0	36.0	40.0	33.0	40.0
17	37.11091311215181	39.0	36.0	40.0	32.0	40.0
18	36.95830793864974	39.0	36.0	40.0	32.0	40.0
19	36.96065692630323	39.0	36.0	40.0	31.0	40.0
20	36.7087275065664	38.0	35.0	40.0	31.0	40.0
21	36.71236013991195	38.0	35.0	40.0	31.0	40.0
22	36.818622197241865	38.0	35.0	40.0	31.0	40.0
23	36.75959474530079	38.0	35.0	40.0	31.0	40.0
24	36.68794074387243	38.0	35.0	40.0	31.0	40.0
25	36.61838285337793	38.0	35.0	40.0	31.0	40.0
26	36.46339955213162	38.0	35.0	40.0	31.0	40.0
27	36.30722694006328	38.0	35.0	40.0	31.0	40.0
28	36.082834906075064	38.0	34.0	40.0	30.0	40.0
29	36.51192175441127	39.0	35.0	40.0	31.0	40.0
30	36.71260758373331	39.0	35.0	40.0	31.0	40.0
31	36.77697557467716	39.0	35.0	40.0	32.0	40.0
32	36.79143943158055	39.0	35.0	40.0	32.0	40.0
33	36.67083452280879	39.0	35.0	40.0	32.0	40.0
34	36.20269847118242	39.0	35.0	40.0	30.0	40.0
35	36.129579068919774	39.0	35.0	40.0	30.0	40.0
36	36.21073027042992	38.0	35.0	40.0	30.0	40.0
37	36.13508061927138	38.0	35.0	40.0	30.0	40.0
38	36.065192309781885	38.0	35.0	40.0	30.0	40.0
39	35.945647310073284	38.0	35.0	40.0	30.0	40.0
40	35.688723575123646	38.0	34.0	40.0	30.0	40.0
41	35.5449814343049	37.0	34.0	40.0	29.0	40.0
42	35.471204651351165	37.0	34.0	40.0	29.0	40.0
43	35.37085902024101	37.0	34.0	40.0	29.0	40.0
44	35.02397933127937	37.0	34.0	40.0	28.0	40.0
45	34.95723370649113	36.0	34.0	40.0	28.0	40.0
46	34.790992452222596	36.0	34.0	40.0	28.0	40.0
47	34.670426067638594	36.0	33.0	40.0	27.0	40.0
48	34.55070128838713	35.0	33.0	39.0	27.0	40.0
49	34.51235688017298	35.0	33.0	39.0	27.0	40.0
50	33.8378536693301	35.0	33.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	0.0
13	4.0
14	15.0
15	28.0
16	86.0
17	246.0
18	663.0
19	1481.0
20	2633.0
21	3192.0
22	3696.0
23	4839.0
24	6718.0
25	9250.0
26	12454.0
27	16926.0
28	23046.0
29	30855.0
30	40692.0
31	52628.0
32	70852.0
33	105498.0
34	187702.0
35	159956.0
36	236512.0
37	352853.0
38	598413.0
39	103461.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.42564387253037	0.4548817554385781	0.05916920119602786	0.060305170835016714
8	99.50091420860947	0.3868223570678549	0.051266803707409776	0.06099663061527079
9	98.31071436685498	1.0024191214312033	0.30122951426926037	0.3856369974445622
10	56.61366462817738	32.14453287446745	4.7771474518225405	6.464655045532626
11	37.052415614742316	22.96698477109224	24.025263964771113	15.955335649394332
12	34.100475032869035	15.443803581959223	24.740480327475353	25.715241057696392
13	27.158613958992483	16.333070249350275	28.877780532641346	27.630535259015893
14	25.740923849534404	18.542778147105103	30.894966271579722	24.821331731780777
15	27.164985266967683	19.5143779183307	28.915958990508233	24.40467782419339
16	35.1267001267347	21.043393052409687	23.037513668678155	20.792393152177457
17	32.102403217856256	20.733421510918642	23.70521686648208	23.45895840474302
18	30.92385941239748	19.792147190055623	25.27715189692113	24.00684150062577
19	31.506661227183063	22.256213506975346	23.157926450410972	23.079198815430615
20	30.727237884883802	23.426064675196645	23.7387032758401	22.107994164079457
21	31.633544096859683	22.00180569782615	24.798908678906823	21.565741526407344
22	28.539014630301153	21.09915434468875	26.912602447174944	23.449228577835157
23	29.591218855910643	22.06023404925762	25.80409363945904	22.544453455372693
24	30.578475252160565	22.158618897990916	23.90188778398006	23.361018065868457
25	29.102653131176837	23.399690423578384	21.871465529248255	25.626190915996528
26	26.147057690465065	23.054207483372863	24.448733690192434	26.350001135969634
27	25.408035355326362	20.77811944671364	25.500641575896104	28.313203622063888
28	26.583516981758304	23.06556717976275	23.132885728368915	27.218030110110032
29	29.400968636372166	22.95646470443552	22.666100986713104	24.976465672479208
30	29.10783907952874	21.23048231295272	25.69207715505788	23.969601452460658
31	30.341304547533415	21.908162287586023	23.74423495408213	24.00629821079843
32	32.59250003210349	21.940858457195183	23.647529364815167	21.81911214588616
33	33.01216672873341	21.722258386666287	22.786513768445925	22.479061116154377
34	29.278333305345676	22.296219394261477	27.195458887283163	21.229988413109684
35	26.52494046037392	26.202522642838304	26.746207590055228	20.526329306732546
36	27.29917785432128	26.835405901708004	24.21319285504731	21.65222338892341
37	29.153919934884243	26.907663448744557	21.75288017693468	22.18553643943652
38	29.132435291712067	26.332319521588854	22.268956122925747	22.266289063773335
39	29.47742433207455	23.875167802471672	22.499854299546303	24.147553565907476
40	27.660959489347075	23.683534663372686	22.536452277915465	26.119053569364777
41	24.603719460937956	25.21877293547396	23.620019143557915	26.557488460030164
42	24.034993791678975	23.994247054628286	23.776239663911035	28.194519489781705
43	24.730701110583187	22.318988177025556	24.578135449068554	28.372175263322703
44	25.739195200083763	22.2364575132538	24.23452932826658	27.78981795839585
45	27.536941238760075	22.061468798865217	23.38521915817735	27.016370804197358
46	26.645945921918386	25.392181170364825	23.000915690308993	24.9609572174078
47	24.614733427437717	24.698202500911247	24.751296734037897	25.935767337613143
48	24.95769747844374	23.413815959089288	26.640809363550783	24.987677198916185
49	28.0870468839365	22.683436871203764	25.572948512916966	23.656567731942772
50	25.694941774147505	22.921150865658255	24.69686897133504	26.687038388859204
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	32.5
8	65.0
9	39.0
10	13.0
11	113.0
12	213.0
13	371.0
14	529.0
15	1830.0
16	3131.0
17	4418.0
18	5705.0
19	5294.0
20	4883.0
21	5018.0
22	5153.0
23	5270.0
24	5387.0
25	5485.0
26	5583.0
27	6554.5
28	7526.0
29	8954.0
30	10382.0
31	11171.0
32	11960.0
33	14913.5
34	17867.0
35	23487.0
36	29107.0
37	32514.5
38	35922.0
39	39686.5
40	43451.0
41	45118.5
42	46786.0
43	50655.0
44	54524.0
45	59731.5
46	64939.0
47	69585.5
48	74232.0
49	78893.5
50	83555.0
51	87420.5
52	91286.0
53	97537.0
54	103788.0
55	107220.0
56	110652.0
57	121260.5
58	131869.0
59	142016.5
60	152164.0
61	161668.5
62	171173.0
63	179131.5
64	187090.0
65	197819.5
66	208549.0
67	167578.5
68	126608.0
69	100827.0
70	75046.0
71	64516.5
72	53987.0
73	45482.0
74	36977.0
75	31337.0
76	25697.0
77	24832.0
78	23967.0
79	16195.0
80	8423.0
81	6187.5
82	3952.0
83	2849.0
84	1746.0
85	1090.0
86	434.0
87	323.0
88	212.0
89	148.5
90	85.0
91	54.5
92	24.0
93	41.0
94	58.0
95	30.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2024702.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.216197052423933
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.18779576531432	5.273795783685649
2	9.780425784296423	1.6071581100077459
3	3.8031785563804257	0.937429933343243
4	1.8366941296667567	0.6036256357748938
5	1.037056479284296	0.4260330194146387
6	0.710281056757446	0.3501485474953851
7	0.5193081073611818	0.29867164187005557
8	0.42533361787638524	0.2795699853994211
9	0.35079415759767857	0.25939745312954243
>10	10.49244875289944	25.10087262123629
>50	4.763654976776268	26.93456262457314
>100	2.014265286898852	27.73610708349093
>500	0.0488454752809664	2.603238616011074
>1k	0.025033306081495284	3.494629496628898
>5k	0.003663410646072481	1.932074307845962
>10k+	0.0012211368820241601	2.1626851400931417
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	27414	1.3539770297061	No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	15697	0.7752745836177373	No Hit
TGCGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	8937	0.44139828972362355	No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	7326	0.3618310250101002	No Hit
TGCGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	6232	0.3077983821816741	No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTG	5488	0.2710522338596001	No Hit
TGCGGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	5403	0.2668540851937717	No Hit
TGCGGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	5128	0.25327183951020943	No Hit
TGCGGAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	3817	0.18852157008784504	No Hit
TGCGGAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	3139	0.15503516072982593	No Hit
TGCGGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	3004	0.14836751284880442	No Hit
TGCGGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2871	0.14179864493639063	No Hit
TGCGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGC	2732	0.1349334371181537	No Hit
TGCGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACT	2665	0.13162430816979487	No Hit
TGCGGAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	2636	0.13019199862498285	No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	2532	0.1250554402573811	No Hit
TGCGGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	2469	0.12194387124623773	No Hit
TGCGGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2373	0.11720243275306687	No Hit
TGCGGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	2306	0.11389330380470804	No Hit
TGCGGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	2028	0.10016288816823414	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.024349262261804453	0.0	0.0	0.0	0.0
7	0.02459621218332377	0.0	0.0	0.0	0.0
8	0.03457298901270409	0.0	0.0	0.0	0.0
9	0.036597978369162475	0.0	0.0	0.0	0.0
10	0.05314362311095658	0.0	0.0	0.0	0.0
11	0.07033133764870089	0.0	0.0	0.0	0.0
12	0.07976482465073872	0.0	0.0	0.0	0.0
13	0.09053184122898086	0.0	0.0	0.0	0.0
14	0.09952081837228392	0.0	0.0	0.0	0.0
15	0.10999149504470287	0.0	0.0	0.0	0.0
16	0.12110424151307204	0.0	0.0	0.0	0.0
17	0.13231576795004896	0.0	0.0	0.0	0.0
18	0.1433297344498104	0.0	0.0	0.0	0.0
19	0.15552906057286456	0.0	0.0	0.0	0.0
20	0.16713570688427234	0.0	0.0	0.0	0.0
21	0.17745821360377972	0.0	0.0	0.0	0.0
22	0.18531122110809392	0.0	0.0	0.0	0.0
23	0.19454714817291632	0.0	0.0	0.0	0.0
24	0.2060550145157164	0.0	0.0	0.0	0.0
25	0.2157848414235774	0.0	0.0	0.0	0.0
26	0.22674941793903497	0.0	0.0	0.0	0.0
27	0.2349975453177801	0.0	0.0	0.0	0.0
28	0.24813528114260766	0.0	0.0	0.0	0.0
29	0.2583096179092034	0.0	0.0	0.0	0.0
30	0.26892846453453395	0.0	0.0	0.0	0.0
31	0.2791521912854336	0.0	0.0	0.0	0.0
32	0.2910551775026646	0.0	0.0	0.0	0.0
33	0.30147646419077967	0.0	0.0	0.0	0.0
34	0.31150263100446385	0.0	0.0	0.0	0.0
35	0.32370195712751804	0.0	0.0	0.0	0.0
36	0.33861773238728465	0.0	0.0	0.0	0.0
37	0.35219997807084696	0.0	0.0	0.0	0.0
38	0.36400418431947024	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATAA	30	2.5286772E-6	44.0	11
ATAACGC	20	7.857904E-4	44.0	12
TCGACGT	20	7.857904E-4	44.0	15
TCGAACG	20	7.857904E-4	44.0	11
CGGAGGG	206505	0.0	42.036564	3
TGCGGAG	210870	0.0	42.02608	1
GCGGAGG	210205	0.0	41.952858	2
GAGGGAT	22490	0.0	41.78924	5
GAGGGGA	31190	0.0	41.637066	5
AGGGATT	7725	0.0	41.60777	6
GGAGGGG	119275	0.0	41.36055	4
GGAGGGA	68230	0.0	41.278618	4
GAGGGGT	22005	0.0	41.030678	5
AGGGGGG	24770	0.0	40.74041	6
GAGGGGG	48700	0.0	40.72033	5
CTATCGA	65	0.0	40.615387	38
AGGGATA	2230	0.0	40.547085	6
AGGGATC	6795	0.0	40.438557	6
AGGGGAG	14195	0.0	40.38887	6
AGGGATG	6270	0.0	40.385963	6
>>END_MODULE