FastQCFastQC Report
Fri 17 Jun 2016
SRR1527722_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527722_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3102955
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC408021.314940113536935No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT231800.7470298473551824No Hit
TGCGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA139180.4485401818589055No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC112520.36262208120968564No Hit
TGCGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC94220.3036460406290133No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT83650.26958173740837366No Hit
TGCGGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG81260.26187940205384863No Hit
TGCGGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT80710.2601068981019705No Hit
TGCGGAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC58760.1893678767497434No Hit
TGCGGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG50020.16120117758717092No Hit
TGCGGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC48980.1578495337508923No Hit
TGCGGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC47230.1522097484494619No Hit
TGCGGAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA46570.15008274370720814No Hit
TGCGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC41330.1331956151474965No Hit
TGCGGAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA40760.13135865650645917No Hit
TGCGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAA39660.1278136486027029No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC38470.12397859459773024No Hit
TGCGGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG37920.1222060906458521No Hit
TGCGGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC36950.11908003822163067No Hit
TGCGGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT35610.11476157404796397No Hit
TGCGGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC31720.10222513700649866No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAG206.313122E-446.040
TGCGGAG3263000.043.5132061
CGGAGGG3203200.043.466073
GAGGGAT350200.043.4648785
GCGGAGG3255000.043.4265442
AGGGATT123050.043.177576
GGAGGGA1047100.042.907274
GAGGGGA469050.042.763675
GAGGGGT328500.042.6252675
GGAGGGG1865450.042.5403564
ATTAGCG601.8189894E-1242.16666839
CGCATAA601.8189894E-1242.16666811
AGGGATC105600.042.0359846
AGGGATG97450.041.964086
AGGGGAT79550.041.8944056
GAGGGGG769150.041.613215
AGGGGTA38650.041.298846
AGGGAGT85600.041.2710276
GAGGGGC322050.041.265025
AGGGGAG216250.041.2245066
Produced by FastQC (version 0.11.5)