##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527722_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3102955 Sequences flagged as poor quality 0 Sequence length 52 %GC 57 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.020256819708955 31.0 31.0 34.0 31.0 34.0 2 32.52241975794041 34.0 31.0 34.0 31.0 34.0 3 32.83743882847157 34.0 31.0 34.0 31.0 34.0 4 36.36666371249341 37.0 37.0 37.0 35.0 37.0 5 36.30896516385188 37.0 37.0 37.0 35.0 37.0 6 34.97972996707977 35.0 35.0 37.0 32.0 37.0 7 36.193423043518195 37.0 35.0 37.0 35.0 37.0 8 36.553341250517654 37.0 37.0 37.0 35.0 37.0 9 38.645581067079604 39.0 39.0 39.0 38.0 39.0 10 37.83749715996526 39.0 38.0 39.0 35.0 39.0 11 37.4066388329834 39.0 37.0 39.0 35.0 39.0 12 36.871550828162185 39.0 35.0 39.0 33.0 39.0 13 36.753836907077286 39.0 35.0 39.0 33.0 39.0 14 37.35309309996439 40.0 36.0 40.0 33.0 40.0 15 37.442814027274004 40.0 36.0 40.0 33.0 40.0 16 37.54001717717466 40.0 36.0 40.0 33.0 40.0 17 37.41501697575376 40.0 36.0 40.0 33.0 40.0 18 37.21756100233487 40.0 36.0 40.0 32.0 40.0 19 37.33095742606644 40.0 36.0 40.0 32.0 40.0 20 37.27109706715051 39.0 36.0 40.0 32.0 40.0 21 37.27487282284145 39.0 36.0 40.0 33.0 40.0 22 37.26210821620037 39.0 36.0 40.0 33.0 40.0 23 37.17501542884122 39.0 35.0 40.0 32.0 40.0 24 37.08460161362314 39.0 35.0 40.0 32.0 40.0 25 37.05052280809744 39.0 35.0 40.0 32.0 40.0 26 36.89202163743915 39.0 35.0 40.0 32.0 40.0 27 36.76962733910096 39.0 35.0 40.0 32.0 40.0 28 36.63560283665087 39.0 35.0 40.0 31.0 40.0 29 36.57778053500615 38.0 35.0 40.0 31.0 40.0 30 36.37629163168657 38.0 35.0 40.0 31.0 40.0 31 36.231503196146896 38.0 35.0 40.0 30.0 40.0 32 36.12302079791682 38.0 35.0 40.0 30.0 40.0 33 35.968644405091275 38.0 35.0 40.0 30.0 40.0 34 35.65647842137575 38.0 34.0 40.0 28.0 40.0 35 35.486839802704196 38.0 34.0 40.0 28.0 40.0 36 35.57029122239929 38.0 34.0 40.0 29.0 40.0 37 35.512125054987905 38.0 34.0 40.0 29.0 40.0 38 35.43100238321213 37.0 34.0 40.0 29.0 40.0 39 35.31944098448092 37.0 34.0 40.0 29.0 40.0 40 35.04705933537547 37.0 34.0 40.0 28.0 40.0 41 34.857440407611456 36.0 34.0 40.0 27.0 40.0 42 34.79520199293899 36.0 33.0 40.0 27.0 40.0 43 34.652218933242665 36.0 33.0 39.0 27.0 40.0 44 34.49525468464738 36.0 33.0 39.0 27.0 40.0 45 34.34494634952811 35.0 33.0 39.0 26.0 40.0 46 34.13718600495334 35.0 33.0 39.0 26.0 40.0 47 33.90516846038695 35.0 33.0 39.0 25.0 40.0 48 33.68844407991737 35.0 32.0 39.0 24.0 40.0 49 34.2102918024915 35.0 33.0 39.0 27.0 40.0 50 34.31408770027281 35.0 33.0 39.0 27.0 40.0 51 34.37883952554903 35.0 33.0 39.0 28.0 40.0 52 33.93329068581401 35.0 33.0 38.0 27.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 4.0 14 5.0 15 31.0 16 99.0 17 267.0 18 630.0 19 1269.0 20 2342.0 21 4005.0 22 6656.0 23 10031.0 24 14359.0 25 20179.0 26 26627.0 27 33869.0 28 42079.0 29 53124.0 30 67642.0 31 86678.0 32 113432.0 33 160977.0 34 293760.0 35 276948.0 36 378502.0 37 529547.0 38 814570.0 39 165323.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 100.0 0.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.47994734051896 0.434231240865562 0.03751262909065713 0.04830878952482392 8 99.52548457840994 0.3631054913783796 0.05101588646951052 0.0603940437421748 9 98.35501965062335 0.9757795391811999 0.29194751454661766 0.3772532956488251 10 56.96382963981108 31.78367072677496 4.821049612385613 6.4314500210283425 11 37.02680187111962 22.77570896129657 23.546812635052717 16.650676532531087 12 33.80290722875453 15.337831196391827 25.256795538446415 25.60246603640723 13 26.99965033331131 16.21844338702946 29.41376848842474 27.36813779123449 14 26.70138625922709 18.22511122462298 30.441208460967044 24.632294055182882 15 26.9699689489535 19.251455467449578 28.692456062044087 25.086119521552842 16 35.64917957237537 20.75067153729268 22.931850445784743 20.668298444547215 17 32.65284221008684 20.436293790918654 23.49215505864571 23.418708940348797 18 30.50173141408754 20.617572604178918 25.008290484393104 23.872405497340438 19 32.115709057978606 21.971507804657172 22.9711033514827 22.941679785881522 20 31.28111751540064 23.169688248782208 23.538562434840337 22.01063180097681 21 32.14564826109306 21.749268036436238 24.55713988762325 21.547943814847457 22 29.000710612947977 20.97813213533551 26.646728682820086 23.374428568896423 23 29.550347974753098 21.998159818624504 25.663182353595204 22.788309853027194 24 30.519005270782205 22.004701969574164 24.020361236305394 23.455931523338236 25 29.056109418280318 23.251932432149356 21.953718310449233 25.738239839121096 26 26.14085605495407 22.938231460011504 24.40969978617157 26.511212698862856 27 25.445776687061205 20.613898686896846 25.55783116416448 28.382493461877466 28 26.711376736046766 22.947674071973328 23.096499949241935 27.24444924273797 29 29.45437494259504 22.81122349502329 22.64180434456832 25.092597217813346 30 29.101872247583355 21.174235527102393 25.642782444476314 24.08110978083794 31 30.599090222062518 21.728288035114915 23.630893777060898 24.04172796576167 32 32.581555323876756 21.85922773614184 23.60878581867929 21.950431121302113 33 33.02603486031863 21.596445968439763 22.862722791661497 22.51479637958011 34 29.139868286842702 22.139025541781944 27.25943495796749 21.461671213407865 35 26.574893931752154 26.05973982864721 26.81830706536189 20.54705917423875 36 27.34538528596129 26.67892380005511 24.274699439727616 21.700991474255993 37 29.150277719141915 26.778055111981963 21.770441401825035 22.301225767051086 38 29.29085339619814 26.162126102376604 22.1705438847808 22.376476616644457 39 29.485474330114357 23.777914923033045 22.467873365872208 24.268737380980387 40 27.69508420199455 23.61942084239056 22.412377878506135 26.273117077108754 41 24.772644140827047 25.137683272880206 23.58390630866384 26.505766277628908 42 24.161065822739936 23.807306261289643 23.72229052628865 28.309337389681772 43 24.76394275779056 22.13689853703969 24.685436946394645 28.4137217587751 44 25.678619251648833 22.012082031482894 24.323233820664495 27.98606489620378 45 27.759377754430858 21.88755557202731 23.3324363389092 27.020630334632635 46 26.569673101930256 25.12424447019051 22.995596133363197 25.310486294516032 47 24.698327884226487 24.586208952434053 24.79275400384472 25.92270915949474 48 25.187377838221952 23.33814057889979 26.538154758931405 24.93632682394685 49 28.074206683628994 22.573385692025827 25.558604620434394 23.79380300391079 50 25.627216637044363 22.92134433145179 24.735711603938825 26.715727427565017 51 24.067993251594043 23.31799848853754 26.264609058139744 26.349399201728673 52 24.558944618919707 23.5465225889515 25.58000357723525 26.314529214893547 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 34.5 8 69.0 9 44.0 10 19.0 11 145.0 12 271.0 13 505.0 14 2515.0 15 4291.0 16 6189.5 17 8088.0 18 7409.0 19 6730.0 20 6711.5 21 6693.0 22 6647.5 23 6602.0 24 6985.0 25 7368.0 26 9060.0 27 10752.0 28 10964.5 29 11177.0 30 14538.0 31 17899.0 32 19891.5 33 21884.0 34 26886.5 35 31889.0 36 39042.0 37 46195.0 38 50509.5 39 58405.5 40 61987.0 41 68411.0 42 74835.0 43 78501.0 44 82167.0 45 89905.5 46 97644.0 47 102557.0 48 107470.0 49 115172.0 50 122874.0 51 128611.0 52 134348.0 53 144127.5 54 153907.0 55 161787.5 56 169668.0 57 177790.5 58 185913.0 59 208666.0 60 231419.0 61 242412.0 62 253405.0 63 260434.5 64 284365.0 65 301266.0 66 255042.0 67 208818.0 68 175037.5 69 141257.0 70 114616.5 71 87976.0 72 77666.0 73 67356.0 74 56472.0 75 45588.0 76 42985.5 77 40383.0 78 29609.0 79 18835.0 80 13253.5 81 7672.0 82 5646.0 83 3620.0 84 2354.0 85 1088.0 86 724.5 87 361.0 88 223.5 89 60.0 90 34.0 91 23.5 92 13.0 93 10.0 94 7.0 95 5.5 96 4.0 97 2.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 3102955.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 7.775565185012678 #Duplication Level Percentage of deduplicated Percentage of total 1 67.64114132733214 5.259481035793261 2 9.86491201302323 1.5341053280335355 3 4.25557833968297 0.9926858034039887 4 2.1444758366520627 0.6669804662229086 5 1.2187095546543887 0.4738077791906486 6 0.7916588139914567 0.369335682748824 7 0.5007179181315988 0.27253553682152615 8 0.3635717699065105 0.2261580797070803 9 0.28896435934482373 0.2022175091007994 >10 5.306688153086301 11.805333198253418 >50 4.574977619217227 25.698129025793055 >100 2.930553721054887 39.45215539417996 >500 0.08657042087639129 4.507903990349242 >1k 0.02733802764517619 4.222933215465449 >5k 0.002485275240470563 1.4448868561072246 >10k+ 0.0016568501603137086 2.871351098829104 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC 40802 1.314940113536935 No Hit TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT 23180 0.7470298473551824 No Hit TGCGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA 13918 0.4485401818589055 No Hit TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC 11252 0.36262208120968564 No Hit TGCGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 9422 0.3036460406290133 No Hit TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT 8365 0.26958173740837366 No Hit TGCGGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG 8126 0.26187940205384863 No Hit TGCGGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT 8071 0.2601068981019705 No Hit TGCGGAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC 5876 0.1893678767497434 No Hit TGCGGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG 5002 0.16120117758717092 No Hit TGCGGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC 4898 0.1578495337508923 No Hit TGCGGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC 4723 0.1522097484494619 No Hit TGCGGAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA 4657 0.15008274370720814 No Hit TGCGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC 4133 0.1331956151474965 No Hit TGCGGAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA 4076 0.13135865650645917 No Hit TGCGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAA 3966 0.1278136486027029 No Hit TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC 3847 0.12397859459773024 No Hit TGCGGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG 3792 0.1222060906458521 No Hit TGCGGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC 3695 0.11908003822163067 No Hit TGCGGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT 3561 0.11476157404796397 No Hit TGCGGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC 3172 0.10222513700649866 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.020142090362251466 0.0 0.0 0.0 0.0 7 0.020528818497206693 0.0 0.0 0.0 0.0 8 0.030164794526507797 0.0 0.0 0.0 0.0 9 0.03200175316754513 0.0 0.0 0.0 0.0 10 0.04743865122117465 0.0 0.0 0.0 0.0 11 0.0603618163975952 0.0 0.0 0.0 0.0 12 0.06983665570399829 0.0 0.0 0.0 0.0 13 0.07873140280796853 0.0 0.0 0.0 0.0 14 0.08707828505408555 0.0 0.0 0.0 0.0 15 0.09716544390750108 0.0 0.0 0.0 0.0 16 0.10763933089587184 0.0 0.0 0.0 0.0 17 0.1167918967564789 0.0 0.0 0.0 0.0 18 0.1262989634074616 0.0 0.0 0.0 0.0 19 0.13835199028023287 0.0 0.0 0.0 0.0 20 0.14934151478187727 0.0 0.0 0.0 0.0 21 0.1579462157846311 0.0 0.0 0.0 0.0 22 0.16580968786205408 0.0 0.0 0.0 0.0 23 0.17463998027686511 0.0 0.0 0.0 0.0 24 0.18359918206999457 0.0 0.0 0.0 0.0 25 0.19230056510648721 0.0 0.0 0.0 0.0 26 0.2019365411357883 0.0 0.0 0.0 0.0 27 0.2099611499361093 0.0 0.0 0.0 0.0 28 0.2211118111606517 0.0 0.0 0.0 0.0 29 0.23110228798032842 0.0 0.0 0.0 0.0 30 0.2403193085300947 0.0 0.0 0.0 0.0 31 0.24914960094490574 0.0 0.0 0.0 0.0 32 0.25891448635252523 0.0 0.0 0.0 0.0 33 0.269227236617998 0.0 0.0 0.0 0.0 34 0.27966889626178915 0.0 0.0 0.0 0.0 35 0.2912385129658664 0.0 0.0 0.0 0.0 36 0.3035493585952745 0.0 0.0 0.0 0.0 37 0.31557015812346617 0.0 0.0 0.0 0.0 38 0.32562508963230213 0.0 0.0 0.0 0.0 39 0.3362278859989913 0.0 0.0 0.0 0.0 40 0.34689513705483965 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGATAG 20 6.313122E-4 46.0 40 TGCGGAG 326300 0.0 43.513206 1 CGGAGGG 320320 0.0 43.46607 3 GAGGGAT 35020 0.0 43.464878 5 GCGGAGG 325500 0.0 43.426544 2 AGGGATT 12305 0.0 43.17757 6 GGAGGGA 104710 0.0 42.90727 4 GAGGGGA 46905 0.0 42.76367 5 GAGGGGT 32850 0.0 42.625267 5 GGAGGGG 186545 0.0 42.540356 4 ATTAGCG 60 1.8189894E-12 42.166668 39 CGCATAA 60 1.8189894E-12 42.166668 11 AGGGATC 10560 0.0 42.035984 6 AGGGATG 9745 0.0 41.96408 6 AGGGGAT 7955 0.0 41.894405 6 GAGGGGG 76915 0.0 41.61321 5 AGGGGTA 3865 0.0 41.29884 6 AGGGAGT 8560 0.0 41.271027 6 GAGGGGC 32205 0.0 41.26502 5 AGGGGAG 21625 0.0 41.224506 6 >>END_MODULE