FastQCFastQC Report
Fri 17 Jun 2016
SRR1527721_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527721_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3119642
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC387591.242418200549935No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT222140.7120688848271692No Hit
TGCGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA133940.42934413628230417No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC104160.33388446494822166No Hit
TGCGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC90470.29000122449947785No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT80560.2582347589883711No Hit
TGCGGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT77540.24855416102232245No Hit
TGCGGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG73820.23662971584560025No Hit
TGCGGAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC57320.18373903159400984No Hit
TGCGGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG46900.15033776311512667No Hit
TGCGGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC46280.14835035558567297No Hit
TGCGGAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA45500.14585006869377962No Hit
TGCGGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC43240.13860564769931935No Hit
TGCGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC38850.12453352019238105No Hit
TGCGGAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA38220.12251405770277488No Hit
TGCGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAA37380.11982144104996663No Hit
TGCGGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC36410.11671210991517617No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC36040.11552607638953442No Hit
TGCGGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG35760.11462853750526503No Hit
TGCGGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT32920.10552492882196098No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC206.3131255E-446.00000424
CGATAGG351.0203257E-746.00000441
AACGCGT253.4185126E-546.043
GAGGGAT341550.043.5353555
TGCGGAG3283300.043.472541
CGGAGGG3218500.043.4495283
AGGGATT115900.043.38056
GCGGAGG3277050.043.368062
GGAGGGA1051600.042.7805254
GAGGGGA473700.042.6837655
GAGGGGT342000.042.6172525
GGAGGGG1870400.042.544594
AGGGATG97950.041.9846886
AGGGATC104550.041.6001936
GAGGGGG761350.041.5984765
AGGGGTG136900.041.3630376
AGGGGGG378900.041.283456
AGGGAGT91600.041.2794766
AGGGGAT84050.041.1017236
GAGGGGC319000.041.097185
Produced by FastQC (version 0.11.5)