##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527721_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3119642
Sequences flagged as poor quality	0
Sequence length	52
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.96558996192512	31.0	31.0	34.0	31.0	34.0
2	32.48316922262234	34.0	31.0	34.0	31.0	34.0
3	32.8149169039268	34.0	31.0	34.0	31.0	34.0
4	36.35049758914645	37.0	37.0	37.0	35.0	37.0
5	36.31067378885141	37.0	37.0	37.0	35.0	37.0
6	34.8567527940706	35.0	35.0	37.0	31.0	37.0
7	36.127234150585224	37.0	35.0	37.0	35.0	37.0
8	36.51684456101053	37.0	37.0	37.0	35.0	37.0
9	38.5991216299819	39.0	39.0	39.0	37.0	39.0
10	37.760691451134456	39.0	38.0	39.0	35.0	39.0
11	37.37743048721616	39.0	37.0	39.0	35.0	39.0
12	36.81775793504511	39.0	35.0	39.0	33.0	39.0
13	36.56158046339932	39.0	35.0	39.0	33.0	39.0
14	37.2901749623835	40.0	36.0	40.0	33.0	40.0
15	37.392454326490025	40.0	36.0	40.0	33.0	40.0
16	37.523691179949495	40.0	36.0	40.0	33.0	40.0
17	37.43535764680691	40.0	36.0	40.0	33.0	40.0
18	37.24951837422371	40.0	36.0	40.0	32.0	40.0
19	37.31304361205549	40.0	36.0	40.0	32.0	40.0
20	37.25943617889489	39.0	36.0	40.0	32.0	40.0
21	37.32614255097219	39.0	36.0	40.0	33.0	40.0
22	37.20886627375833	39.0	36.0	40.0	32.0	40.0
23	37.13847967170592	39.0	35.0	40.0	32.0	40.0
24	37.09742271709382	39.0	35.0	40.0	32.0	40.0
25	37.03558613456288	39.0	35.0	40.0	32.0	40.0
26	36.83263079545666	39.0	35.0	40.0	32.0	40.0
27	36.69852149701793	39.0	35.0	40.0	31.0	40.0
28	36.594110157511665	38.0	35.0	40.0	31.0	40.0
29	36.53690038792913	38.0	35.0	40.0	31.0	40.0
30	36.31920297264878	38.0	35.0	40.0	31.0	40.0
31	36.19435210835089	38.0	35.0	40.0	30.0	40.0
32	36.08827551366471	38.0	35.0	40.0	30.0	40.0
33	35.78270936216399	38.0	35.0	40.0	30.0	40.0
34	35.561926977518574	38.0	34.0	40.0	28.0	40.0
35	35.465730683200185	38.0	34.0	40.0	28.0	40.0
36	35.408744336689914	38.0	34.0	40.0	28.0	40.0
37	35.41764952516988	38.0	34.0	40.0	29.0	40.0
38	35.06666630337712	37.0	34.0	40.0	28.0	40.0
39	35.11781928823884	37.0	34.0	40.0	28.0	40.0
40	34.92140893089656	37.0	34.0	40.0	27.0	40.0
41	34.54823886843426	36.0	33.0	40.0	26.0	40.0
42	34.61868477216296	36.0	33.0	39.0	27.0	40.0
43	34.59373799942429	36.0	33.0	39.0	27.0	40.0
44	34.43931963988175	36.0	33.0	39.0	27.0	40.0
45	34.28030395795415	35.0	33.0	39.0	26.0	40.0
46	33.8993660169981	35.0	33.0	39.0	25.0	40.0
47	33.5911742437113	35.0	32.0	39.0	23.0	40.0
48	33.53738185343062	35.0	32.0	38.0	24.0	40.0
49	33.540128322416486	35.0	32.0	38.0	25.0	40.0
50	33.3456313256457	35.0	32.0	38.0	24.0	40.0
51	33.85742370438659	35.0	33.0	39.0	26.0	40.0
52	33.67647056937943	35.0	33.0	38.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	3.0
13	3.0
14	9.0
15	30.0
16	93.0
17	292.0
18	653.0
19	1389.0
20	2485.0
21	4519.0
22	7139.0
23	10577.0
24	15415.0
25	21159.0
26	28044.0
27	35177.0
28	44330.0
29	55718.0
30	70849.0
31	91150.0
32	120189.0
33	173846.0
34	297608.0
35	286832.0
36	394966.0
37	547170.0
38	774477.0
39	135516.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.46727861722596	0.43899267928820035	0.0420561077200525	0.051672595765796206
8	99.51766901458565	0.3694654707174734	0.05157643088533877	0.06128908381153992
9	98.31368471125853	0.9938640395276125	0.31320901564987264	0.37924223356397946
10	56.96118336655296	31.804803243449086	4.818405445240191	6.415607944757763
11	36.950073117364106	22.738987358164813	23.61613928777725	16.694800236693826
12	33.82317586441008	15.268546839669423	25.292517538871444	25.615759757049045
13	27.228380692399963	16.234106349382397	29.293457390303118	27.244055567914522
14	26.59439769050423	18.26177490878761	30.452532694456607	24.69129470625155
15	26.935398356606306	19.265800370683557	28.728072003133693	25.070729269576447
16	35.68169039909066	20.783698898783896	22.89435775002388	20.640252952101555
17	32.62201239757639	20.461899153813164	23.46766071235097	23.448427736259482
18	30.54898606955542	20.536939815530115	25.025339446000533	23.888734668913933
19	32.1010551851783	21.972072436516754	23.006229560956033	22.920642817348913
20	31.24143731876927	23.194007517529254	23.57395496021659	21.990600203484885
21	32.11733910493576	21.727300760792424	24.569549967592437	21.58581016667938
22	29.093274164150884	20.956571298886217	26.56182985098931	23.388324685973583
23	29.55550668954963	21.98015028647518	25.638679053558068	22.825663970417118
24	30.465450843398056	22.003005473063897	23.98605352793686	23.545490155601186
25	29.05269258459785	23.300782589797162	21.94530654478943	25.701218280815556
26	26.19300547947489	22.878554654668708	24.403280889281525	26.525158976574875
27	25.440675564696207	20.649709165346536	25.518440898026117	28.39117437193114
28	26.744062299456157	22.879676578274047	23.123807154795326	27.252453967474473
29	29.458444270207927	22.809347995699504	22.54858089485909	25.183626839233476
30	29.143824836311342	21.100433960050545	25.629383115113853	24.12635808852426
31	30.627648941769603	21.727461035593187	23.636878846995906	24.008011175641307
32	32.61239590953065	21.833370623936975	23.57321769613308	21.981015770399296
33	33.00994152534169	21.622705425814885	22.88749798855125	22.479855060292174
34	29.16286548264192	22.16558823095727	27.21325075120799	21.45829553519282
35	26.571189899353836	26.052444479206265	26.79349104801128	20.582874573428615
36	27.50389307491052	26.545417711391245	24.29249894699456	21.65819026670368
37	29.190432748373052	26.773296423115216	21.755412960846147	22.280857867665585
38	29.281244450485023	26.13703751904866	22.12638501469079	22.455333015775526
39	29.46145743646226	23.796512548555253	22.493189923715605	24.24884009126688
40	27.68497154481187	23.56231900968124	22.451646695357994	26.301062750148894
41	25.07803780049121	25.108938782078198	23.51138367799895	26.301639739431643
42	24.225696410036793	23.826868595819647	23.672331632924546	28.27510336121901
43	24.790536862883624	22.170300310099684	24.665073748846822	28.374089078169867
44	25.68422915193474	22.027142858058713	24.309231636194152	27.97939635381239
45	27.711096337336144	21.89975644641276	23.356878770064	27.032268446187096
46	26.775283830644668	25.083262758996067	22.989496871756437	25.151956538602825
47	24.949273025558703	24.576730278666588	24.7048860093562	25.769110686418507
48	25.161861521289943	23.287992660696325	26.598051955961616	24.95209386205212
49	28.14463967339842	22.533899723109254	25.532256585851837	23.789204017640486
50	25.708174207168643	22.800244387016203	24.74883335972525	26.742748046089904
51	24.13411538888116	23.28132522898461	26.24987097878539	26.334688403348842
52	24.58602621711081	23.4357339720391	25.586782073071205	26.391457737778882
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	30.0
8	60.0
9	41.5
10	23.0
11	169.0
12	315.0
13	497.5
14	2389.5
15	4099.0
16	5963.5
17	7828.0
18	7403.5
19	6979.0
20	6815.5
21	6652.0
22	6622.5
23	6593.0
24	7083.0
25	7573.0
26	9030.0
27	10487.0
28	10903.0
29	11319.0
30	14593.5
31	17868.0
32	19837.5
33	21807.0
34	26460.5
35	31114.0
36	38655.0
37	46196.0
38	50751.0
39	58947.0
40	62588.0
41	68662.0
42	74736.0
43	78412.0
44	82088.0
45	90131.0
46	98174.0
47	103108.0
48	108042.0
49	115793.5
50	123545.0
51	129817.5
52	136090.0
53	145411.0
54	154732.0
55	162853.5
56	170975.0
57	179309.0
58	187643.0
59	210331.5
60	233020.0
61	244036.0
62	255052.0
63	261413.5
64	284728.5
65	301682.0
66	255637.5
67	209593.0
68	176498.0
69	143403.0
70	116532.0
71	89661.0
72	78691.0
73	67721.0
74	56966.5
75	46212.0
76	43232.0
77	40252.0
78	29518.0
79	18784.0
80	13259.0
81	7734.0
82	5717.5
83	3701.0
84	2374.5
85	1048.0
86	688.0
87	328.0
88	223.5
89	71.5
90	24.0
91	18.0
92	12.0
93	9.5
94	7.0
95	4.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3119642.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.194255122031878
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.48214531182046	5.529659148670843
2	10.073255906149186	1.650856576090017
3	4.26380423769628	1.0481609914225192
4	2.208985033558496	0.7240394770291387
5	1.2504910088960641	0.5123421177350692
6	0.7703041471027893	0.37872412217516566
7	0.5174673704831005	0.296818175574586
8	0.3586247327757627	0.23509300427480856
9	0.3001814247104493	0.22137868592751828
>10	5.528628691209166	12.948889455226793
>50	4.44836810519079	26.049148766884823
>100	2.692045105258063	37.914744149887916
>500	0.07784029371465936	4.292449912552762
>1k	0.0241714596271837	4.076758190255056
>5k	0.00204842878196472	1.2747129007573803
>10k+	0.0016387430255717763	2.8462243255356188
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	38759	1.242418200549935	No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	22214	0.7120688848271692	No Hit
TGCGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	13394	0.42934413628230417	No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	10416	0.33388446494822166	No Hit
TGCGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	9047	0.29000122449947785	No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT	8056	0.2582347589883711	No Hit
TGCGGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	7754	0.24855416102232245	No Hit
TGCGGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	7382	0.23662971584560025	No Hit
TGCGGAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	5732	0.18373903159400984	No Hit
TGCGGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	4690	0.15033776311512667	No Hit
TGCGGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	4628	0.14835035558567297	No Hit
TGCGGAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	4550	0.14585006869377962	No Hit
TGCGGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	4324	0.13860564769931935	No Hit
TGCGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	3885	0.12453352019238105	No Hit
TGCGGAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	3822	0.12251405770277488	No Hit
TGCGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAA	3738	0.11982144104996663	No Hit
TGCGGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	3641	0.11671210991517617	No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	3604	0.11552607638953442	No Hit
TGCGGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	3576	0.11462853750526503	No Hit
TGCGGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	3292	0.10552492882196098	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.01897653641026759	0.0	0.0	0.0	0.0
7	0.019104756250877503	0.0	0.0	0.0	0.0
8	0.027823705412351803	0.0	0.0	0.0	0.0
9	0.029779057981653024	0.0	0.0	0.0	0.0
10	0.04551804341652023	0.0	0.0	0.0	0.0
11	0.05869263203918911	0.0	0.0	0.0	0.0
12	0.06805268040371298	0.0	0.0	0.0	0.0
13	0.0762908051629001	0.0	0.0	0.0	0.0
14	0.0842404352807149	0.0	0.0	0.0	0.0
15	0.09453007748966068	0.0	0.0	0.0	0.0
16	0.10408245561509942	0.0	0.0	0.0	0.0
17	0.11369894366084314	0.0	0.0	0.0	0.0
18	0.12257816762307983	0.0	0.0	0.0	0.0
19	0.13437439295919212	0.0	0.0	0.0	0.0
20	0.14527307941103498	0.0	0.0	0.0	0.0
21	0.15450490793494895	0.0	0.0	0.0	0.0
22	0.16277508765428852	0.0	0.0	0.0	0.0
23	0.17194280625789754	0.0	0.0	0.0	0.0
24	0.18127079966226894	0.0	0.0	0.0	0.0
25	0.18966919922221845	0.0	0.0	0.0	0.0
26	0.19842020334384522	0.0	0.0	0.0	0.0
27	0.20678654794364226	0.0	0.0	0.0	0.0
28	0.2178775641564	0.0	0.0	0.0	0.0
29	0.22650034843741687	0.0	0.0	0.0	0.0
30	0.23518724263873866	0.0	0.0	0.0	0.0
31	0.24384208187990802	0.0	0.0	0.0	0.0
32	0.2547407683317509	0.0	0.0	0.0	0.0
33	0.2632673877323103	0.0	0.0	0.0	0.0
34	0.27265949105698667	0.0	0.0	0.0	0.0
35	0.28461599119386133	0.0	0.0	0.0	0.0
36	0.29807907445790255	0.0	0.0	0.0	0.0
37	0.31077283867828426	0.0	0.0	0.0	0.0
38	0.320325216803723	0.0	0.0	0.0	0.0
39	0.33023019949083904	0.0	0.0	0.0	0.0
40	0.3412891607434443	0.0	0.0	0.0	3.205496015247904E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAC	20	6.3131255E-4	46.000004	24
CGATAGG	35	1.0203257E-7	46.000004	41
AACGCGT	25	3.4185126E-5	46.0	43
GAGGGAT	34155	0.0	43.535355	5
TGCGGAG	328330	0.0	43.47254	1
CGGAGGG	321850	0.0	43.449528	3
AGGGATT	11590	0.0	43.3805	6
GCGGAGG	327705	0.0	43.36806	2
GGAGGGA	105160	0.0	42.780525	4
GAGGGGA	47370	0.0	42.683765	5
GAGGGGT	34200	0.0	42.617252	5
GGAGGGG	187040	0.0	42.54459	4
AGGGATG	9795	0.0	41.984688	6
AGGGATC	10455	0.0	41.600193	6
GAGGGGG	76135	0.0	41.598476	5
AGGGGTG	13690	0.0	41.363037	6
AGGGGGG	37890	0.0	41.28345	6
AGGGAGT	9160	0.0	41.279476	6
AGGGGAT	8405	0.0	41.101723	6
GAGGGGC	31900	0.0	41.09718	5
>>END_MODULE