FastQCFastQC Report
Fri 17 Jun 2016
SRR1527720_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527720_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235671
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTGATCAGGGA20480.8690080663297564No Hit
TGATCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14320.6076267338790093No Hit
TGATCAGGGGAGTGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAAAA11840.5023952883468904No Hit
TGATCAGGGGAAGGCAGTTTTAACTTTTTCAAAGATTGCAAACTTCTTGT11590.491787279724701No Hit
TGATCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9280.3937692800556708No Hit
TGATCAGGGAGTGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAAAAA8980.38103966970904357No Hit
TGATCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8530.3619452541891026No Hit
TGATCAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA7160.30381336693950467No Hit
TGATCAGGGGAGTGCAGTGCTTGATCAGGGAAAAAAAAAAAAAAAAAAAA6890.29235671762754006No Hit
TGATCAGGGGAGTGCAGTGCTTAATCAGGGGAAAAAAAAAAAAAAAAAAA6250.2652002155547352No Hit
TGATCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5860.24865172210411973Illumina Single End Adapter 2 (100% over 34bp)
TGATCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5100.2164033758926639No Hit
TGATCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4940.2096142503744627Illumina Single End Adapter 2 (97% over 34bp)
TGATCAGGGCAGAGTGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAA4850.2057953672704745No Hit
TGATCAGGGGCCCGTCTGCCCCAGCCCAGACACCGAAGCTACTCTCCTTC4830.20494672658069937No Hit
TGATCAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA4750.20155216382159877No Hit
TGATCAGGGGTGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAAAAAA4720.20027920278693603No Hit
TGATCAGGGAGTGCAGTGCTTAATCAGGGGAAAAAAAAAAAAAAAAAAAA4480.1900955145096342No Hit
TGATCAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA4150.17609294312834417Illumina Single End Adapter 2 (100% over 34bp)
TGATCAGGGGAGTCCAGAGCCTGCAACCTACTTCCTTCTAGCTGCAGCCA3610.15317964450441507No Hit
TGATCAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA3430.1455418782964387Illumina Single End Adapter 2 (100% over 34bp)
TGATCAGGGAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGT3420.1451175579515511No Hit
TGATCAGGGGAGTGCAGTGCTTAATCAGGGGGAAAAAAAAAAAAAAAAAA3400.14426891726177596No Hit
TGATCAGGGGGCGCCAGTCCTCCGATAGACTGAGTCGCCCGGGTACCCGT3370.14299595622711322No Hit
TGATCAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA3360.14257163588222566Illumina Single End Adapter 2 (97% over 34bp)
TGATCAGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA3350.1421473155373381Illumina Single End Adapter 1 (96% over 33bp)
TGATCAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA3310.14045003415778776No Hit
TGATCAGGGAGTGCAGTGCTTGATCAGGGAAAAAAAAAAAAAAAAAAAAA3240.13747979174357472No Hit
TGATCAGGGGACACCACCCACCCAGAGTGGAGAAGCCCAGCCAGTCGCTG3210.136206830708912No Hit
TGATCAGGGAGAGTGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAAA3140.13323658829469898No Hit
TGATCAGGGAGTGCAGTGCTTGATCAGGGATTGCCACTTCTTCCCCTGTT3110.13196362726003624No Hit
TGATCAGGGGATCGAGCATCGGTCGGAGCTGCTGCCAGCCAACTCTCGAA3100.13153930691514867No Hit
TGATCAGGGATTAAGAGCCTTGTCTGTTTAGTGCTGTGGATGGCTCCCAG3030.1285690645009356No Hit
TGATCAGGGATTAGTTTGTGACTCTCTTGAGGGCAAGGGTGAGGTAGGGT3000.1272961034662729No Hit
TGATCAGGGGCAGTGCATCGTGGGTGAGAACGAGCGGAGCAGGTTTGGCG2940.12475018139694743No Hit
TGATCAGGGGTGCAGTGCTTAATCAGGGGAAAAAAAAAAAAAAAAAAAAA2820.11965833725829653No Hit
TGATCAGGGGAGTGCAGTGCTTGATCGGAAGAGCTCGTATGCCGTCTTCT2810.11923401691340894Illumina Single End Adapter 2 (96% over 29bp)
TGATCAGGGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAAAAAAAAA2750.11668809484408349No Hit
TGATCAGGGCAGAGTGCAGTGCTTAATCAGGGGAAAAAAAAAAAAAAAAA2710.11499081346453319No Hit
TGATCAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA2690.11414217277475804No Hit
TGATCAGGGGGTACAGTGAAACTGCAAATGGCTGAGGACATTCTGTATCC2690.11414217277475804No Hit
TGATCAGGGAGCAGACCGTAACCATTATAGACGCTATCCACGTCGTAGGG2660.1128692117400953No Hit
TGATCAGGGGCCGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAA2600.11032328967076985No Hit
TGATCAGGGGGCCCGTCCGCCCCAGCCCAGACACCGAAGCTACTCTCCTT2580.1094746489809947No Hit
TGATCAGGGAGAGTGCAGTGCTTGATCAGGGAAGTAATTTACACCGGAAA2450.10395848449745619No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAAA650.044.00000431
GCCGTTA650.044.00000429
CCGTTAA650.044.00000430
AGGTCAC254.4320408E-544.032
TCACGCG254.4320408E-544.014
ACAACTA254.4320408E-544.015
TTTAGTA207.8437326E-444.016
GTGTTGT207.8437326E-444.043
TCGCCCG454.765752E-1044.035
AACGTTT351.43953E-744.017
CGGGTTT207.8437326E-444.021
CTAGATA254.4320408E-544.019
AGCGTTT207.8437326E-444.026
GTCATAA207.8437326E-444.011
AAACTCT254.4320408E-544.021
CTAGACA207.8437326E-444.034
CGGGTAC351.43953E-744.040
AGCGTCG207.8437326E-444.040
AAGTACT254.4320408E-544.031
AACAAGG207.8437326E-444.033
Produced by FastQC (version 0.11.5)