##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527720_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	235671
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37597328479109	34.0	31.0	34.0	30.0	34.0
2	32.49833878584977	34.0	31.0	34.0	30.0	34.0
3	32.573405298063825	34.0	31.0	34.0	30.0	34.0
4	36.22962519783936	37.0	37.0	37.0	35.0	37.0
5	36.29184329001023	37.0	37.0	37.0	35.0	37.0
6	36.27432310296982	37.0	37.0	37.0	35.0	37.0
7	36.55260935796088	37.0	37.0	37.0	35.0	37.0
8	36.648162056426116	37.0	37.0	37.0	35.0	37.0
9	38.63012419856495	39.0	39.0	39.0	38.0	39.0
10	38.059239363349754	39.0	38.0	39.0	37.0	39.0
11	37.83057737269329	39.0	38.0	39.0	35.0	39.0
12	37.63630230278651	39.0	37.0	39.0	35.0	39.0
13	37.40329102859495	39.0	37.0	39.0	35.0	39.0
14	38.170928964531065	40.0	38.0	40.0	34.0	40.0
15	38.20433570528406	40.0	38.0	40.0	34.0	40.0
16	38.254095752129025	40.0	38.0	40.0	35.0	40.0
17	38.35997216458537	40.0	38.0	40.0	35.0	40.0
18	38.24602093596582	40.0	38.0	40.0	35.0	40.0
19	38.23029986718773	40.0	38.0	40.0	34.0	40.0
20	38.316046522482615	40.0	38.0	40.0	35.0	40.0
21	38.32955263906039	40.0	38.0	40.0	35.0	40.0
22	38.37885017672942	40.0	38.0	40.0	35.0	40.0
23	38.25578454710168	40.0	38.0	40.0	35.0	40.0
24	38.11565275320256	40.0	38.0	40.0	35.0	40.0
25	37.93186263901795	40.0	37.0	40.0	34.0	40.0
26	37.655549473630614	40.0	37.0	40.0	34.0	40.0
27	37.50964692304102	40.0	36.0	40.0	34.0	40.0
28	37.412927343627345	40.0	36.0	40.0	33.0	40.0
29	37.351299056735876	40.0	36.0	40.0	33.0	40.0
30	37.354638457850136	40.0	36.0	40.0	33.0	40.0
31	37.29770739717657	40.0	36.0	40.0	33.0	40.0
32	37.201726135163	40.0	36.0	40.0	33.0	40.0
33	37.06612183934383	40.0	36.0	40.0	33.0	40.0
34	36.682553220379255	40.0	35.0	40.0	31.0	40.0
35	36.56610274492831	40.0	35.0	40.0	31.0	40.0
36	36.33608292916821	40.0	35.0	40.0	30.0	40.0
37	35.960385452601294	40.0	35.0	40.0	27.0	40.0
38	35.72114091254333	40.0	35.0	40.0	25.0	40.0
39	35.40772093299557	40.0	35.0	40.0	23.0	40.0
40	35.01667578955408	39.0	35.0	40.0	21.0	40.0
41	34.726584942568245	39.0	35.0	40.0	20.0	40.0
42	34.43570061653746	38.0	35.0	40.0	18.0	40.0
43	34.17410712391427	38.0	35.0	40.0	17.0	40.0
44	33.769335217315664	37.0	34.0	40.0	15.0	40.0
45	33.583546554306636	37.0	34.0	40.0	14.0	40.0
46	33.38145974685048	37.0	34.0	40.0	12.0	40.0
47	33.19999915135931	36.0	33.0	40.0	12.0	40.0
48	32.98190273729055	36.0	33.0	40.0	11.0	40.0
49	32.7199400859673	35.0	33.0	40.0	10.0	40.0
50	31.98432560645985	35.0	31.0	40.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	7.0
16	15.0
17	32.0
18	67.0
19	115.0
20	216.0
21	325.0
22	475.0
23	774.0
24	1297.0
25	1931.0
26	2664.0
27	3612.0
28	4990.0
29	5805.0
30	5654.0
31	5242.0
32	5551.0
33	6903.0
34	10134.0
35	13739.0
36	20843.0
37	35080.0
38	67653.0
39	42542.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	100.0	0.0	0.0
7	99.24683138782456	0.5881079980141808	0.1306906662253735	0.03436994793589368
8	97.14771864166572	2.163609438581752	0.45996325385813275	0.22870866589440364
9	94.55299973267817	3.468818819455936	1.186824004650551	0.7913574432153299
10	47.6248668694918	36.88574326073212	5.478824293188386	10.0105655765877
11	28.7816489937243	37.278239579753134	17.944931705640492	15.995179720882078
12	37.367771172524414	24.834196825235182	20.99664362607194	16.80138837616847
13	24.488375744151806	28.46468169609328	24.55244811622983	22.494494443525085
14	27.828625499106806	31.764196697939077	20.602025705326493	19.805152097627627
15	25.5678467015458	32.61920219288754	20.876985288813643	20.935965816753015
16	26.778008325165164	31.702245927585487	25.03447602802212	16.485269719227226
17	30.623199290536384	29.575552359008956	20.790423938456577	19.01082441199808
18	28.204997645022083	30.400006789125516	23.32107047536608	18.073925090486316
19	25.952280934013945	29.24118792723755	24.964463171115668	19.842067967632847
20	25.93148923711445	28.985747079615226	23.977494048907165	21.105269634363157
21	22.69647092769157	27.82098773289883	29.062549062039878	20.419992277369722
22	22.713019421142185	32.29926465284231	23.62191359989137	21.365802326124133
23	21.778241701354855	36.161852752353916	23.217536311213514	18.84236923507771
24	21.498190273729055	34.99412316322331	24.31610168412745	19.19158487892019
25	22.443151681793687	31.44213755616941	22.935787602208162	23.17892315982874
26	25.673502467422804	32.61877787254265	20.225653559411214	21.482066100623324
27	23.820071200953873	33.40334619023979	21.036954058836262	21.739628549970085
28	29.62986536315457	31.797718005185192	19.225954826856086	19.346461804804154
29	31.070432934047886	32.7061878635895	18.941660195781406	17.28171900658121
30	29.348540974494103	34.878283709068995	19.112236974426214	16.660938342010684
31	28.198208519503886	34.06486160791952	18.71210288919723	19.024826983379374
32	24.57154253174977	37.047833632479175	20.502310424277916	17.878313411493142
33	27.177293769704374	37.28715030699577	18.81139384990092	16.724162073398933
34	24.254150913773863	38.363651021975556	17.476057724539718	19.906140339710866
35	23.523895600222346	42.80161750915471	18.188915903950846	15.485570986672098
36	24.14043306134399	40.21920389016892	18.423565054673677	17.216797993813408
37	22.62476078940557	41.62327991140191	18.666276291949373	17.085683007243148
38	24.646647232794873	40.748755680588616	18.18170245808776	16.422894628528752
39	22.914147264618897	38.57920575717844	17.924140008741	20.582506969461665
40	22.890385325305193	40.58327074608246	19.525524990346714	17.00081893826563
41	22.32688790729449	40.8552600871554	20.169218953541165	16.648633052008947
42	22.660403698376125	40.693169715408345	18.913655053018825	17.732771533196704
43	21.708228844448403	41.130219670642546	20.039376928005566	17.12217455690348
44	21.958153527587186	39.08499560828443	21.17443385057983	17.78241701354855
45	19.783936080383246	39.86574504287757	19.284935354795458	21.065383521943726
46	20.89141218053982	41.24563480445197	19.323548506180227	18.539404508827985
47	22.921360710481984	39.33322301004366	19.58026231483721	18.165153964637142
48	23.34652969605934	38.54101692613855	19.272630064793717	18.839823313008388
49	22.86450178426705	39.34467965935563	19.82467083349245	17.96614772288487
50	20.44672445909764	41.64025272519741	20.007128581794113	17.905894233910832
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	9.5
4	19.0
5	39.5
6	60.0
7	86.5
8	113.0
9	680.0
10	1247.0
11	1722.0
12	2197.0
13	2017.5
14	1838.0
15	1574.0
16	1310.0
17	1247.5
18	1185.0
19	1203.5
20	1222.0
21	1559.0
22	1896.0
23	2440.5
24	2985.0
25	3813.5
26	4642.0
27	5023.5
28	5405.0
29	6512.5
30	7620.0
31	8962.0
32	10304.0
33	10683.5
34	11063.0
35	11148.0
36	11233.0
37	10438.0
38	9643.0
39	9457.0
40	9271.0
41	9229.0
42	9187.0
43	9428.0
44	9669.0
45	9551.0
46	9433.0
47	10728.0
48	12023.0
49	12125.0
50	12227.0
51	15372.0
52	18517.0
53	18495.0
54	18473.0
55	16696.0
56	14919.0
57	13706.0
58	12493.0
59	11479.0
60	10465.0
61	8954.0
62	7443.0
63	6734.0
64	6025.0
65	5338.0
66	4651.0
67	3398.0
68	2145.0
69	2261.5
70	2378.0
71	1892.5
72	1407.0
73	1001.0
74	595.0
75	399.5
76	204.0
77	122.5
78	41.0
79	61.0
80	81.0
81	57.0
82	33.0
83	20.5
84	8.0
85	4.0
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	235671.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.94798256892023
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.35334642783853	22.39223324040718
2	9.253613203951804	5.357468674550539
3	3.7568526281844568	3.2625991318405747
4	1.9421887367711295	2.2488978279041545
5	1.1198733546363342	1.6209037174705416
6	0.7988625370115212	1.3875275277823746
7	0.6112397760253291	1.2385910867268353
8	0.4397408460613878	1.0183688277301832
9	0.34592946556829174	0.901256412541212
>10	3.1763947113834248	21.96918585655426
>50	0.831110199056023	16.436897199910046
>100	0.3532584796693149	17.232073526229364
>500	0.01172642256163701	2.4631796020723806
>1k	0.005863211280818505	2.470817368280357
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTGATCAGGGA	2048	0.8690080663297564	No Hit
TGATCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1432	0.6076267338790093	No Hit
TGATCAGGGGAGTGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAAAA	1184	0.5023952883468904	No Hit
TGATCAGGGGAAGGCAGTTTTAACTTTTTCAAAGATTGCAAACTTCTTGT	1159	0.491787279724701	No Hit
TGATCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	928	0.3937692800556708	No Hit
TGATCAGGGAGTGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAAAAA	898	0.38103966970904357	No Hit
TGATCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	853	0.3619452541891026	No Hit
TGATCAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	716	0.30381336693950467	No Hit
TGATCAGGGGAGTGCAGTGCTTGATCAGGGAAAAAAAAAAAAAAAAAAAA	689	0.29235671762754006	No Hit
TGATCAGGGGAGTGCAGTGCTTAATCAGGGGAAAAAAAAAAAAAAAAAAA	625	0.2652002155547352	No Hit
TGATCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	586	0.24865172210411973	Illumina Single End Adapter 2 (100% over 34bp)
TGATCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	510	0.2164033758926639	No Hit
TGATCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	494	0.2096142503744627	Illumina Single End Adapter 2 (97% over 34bp)
TGATCAGGGCAGAGTGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAA	485	0.2057953672704745	No Hit
TGATCAGGGGCCCGTCTGCCCCAGCCCAGACACCGAAGCTACTCTCCTTC	483	0.20494672658069937	No Hit
TGATCAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA	475	0.20155216382159877	No Hit
TGATCAGGGGTGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAAAAAA	472	0.20027920278693603	No Hit
TGATCAGGGAGTGCAGTGCTTAATCAGGGGAAAAAAAAAAAAAAAAAAAA	448	0.1900955145096342	No Hit
TGATCAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	415	0.17609294312834417	Illumina Single End Adapter 2 (100% over 34bp)
TGATCAGGGGAGTCCAGAGCCTGCAACCTACTTCCTTCTAGCTGCAGCCA	361	0.15317964450441507	No Hit
TGATCAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA	343	0.1455418782964387	Illumina Single End Adapter 2 (100% over 34bp)
TGATCAGGGAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGT	342	0.1451175579515511	No Hit
TGATCAGGGGAGTGCAGTGCTTAATCAGGGGGAAAAAAAAAAAAAAAAAA	340	0.14426891726177596	No Hit
TGATCAGGGGGCGCCAGTCCTCCGATAGACTGAGTCGCCCGGGTACCCGT	337	0.14299595622711322	No Hit
TGATCAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	336	0.14257163588222566	Illumina Single End Adapter 2 (97% over 34bp)
TGATCAGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA	335	0.1421473155373381	Illumina Single End Adapter 1 (96% over 33bp)
TGATCAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA	331	0.14045003415778776	No Hit
TGATCAGGGAGTGCAGTGCTTGATCAGGGAAAAAAAAAAAAAAAAAAAAA	324	0.13747979174357472	No Hit
TGATCAGGGGACACCACCCACCCAGAGTGGAGAAGCCCAGCCAGTCGCTG	321	0.136206830708912	No Hit
TGATCAGGGAGAGTGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAAA	314	0.13323658829469898	No Hit
TGATCAGGGAGTGCAGTGCTTGATCAGGGATTGCCACTTCTTCCCCTGTT	311	0.13196362726003624	No Hit
TGATCAGGGGATCGAGCATCGGTCGGAGCTGCTGCCAGCCAACTCTCGAA	310	0.13153930691514867	No Hit
TGATCAGGGATTAAGAGCCTTGTCTGTTTAGTGCTGTGGATGGCTCCCAG	303	0.1285690645009356	No Hit
TGATCAGGGATTAGTTTGTGACTCTCTTGAGGGCAAGGGTGAGGTAGGGT	300	0.1272961034662729	No Hit
TGATCAGGGGCAGTGCATCGTGGGTGAGAACGAGCGGAGCAGGTTTGGCG	294	0.12475018139694743	No Hit
TGATCAGGGGTGCAGTGCTTAATCAGGGGAAAAAAAAAAAAAAAAAAAAA	282	0.11965833725829653	No Hit
TGATCAGGGGAGTGCAGTGCTTGATCGGAAGAGCTCGTATGCCGTCTTCT	281	0.11923401691340894	Illumina Single End Adapter 2 (96% over 29bp)
TGATCAGGGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAAAAAAAAA	275	0.11668809484408349	No Hit
TGATCAGGGCAGAGTGCAGTGCTTAATCAGGGGAAAAAAAAAAAAAAAAA	271	0.11499081346453319	No Hit
TGATCAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA	269	0.11414217277475804	No Hit
TGATCAGGGGGTACAGTGAAACTGCAAATGGCTGAGGACATTCTGTATCC	269	0.11414217277475804	No Hit
TGATCAGGGAGCAGACCGTAACCATTATAGACGCTATCCACGTCGTAGGG	266	0.1128692117400953	No Hit
TGATCAGGGGCCGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAA	260	0.11032328967076985	No Hit
TGATCAGGGGGCCCGTCCGCCCCAGCCCAGACACCGAAGCTACTCTCCTT	258	0.1094746489809947	No Hit
TGATCAGGGAGAGTGCAGTGCTTGATCAGGGAAGTAATTTACACCGGAAA	245	0.10395848449745619	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.4569930114439197	0.0	0.0	0.0	0.0
7	0.5354922752481214	0.0	0.0	0.0	0.0
8	0.5982916862914827	0.0	0.0	0.0	0.0
9	0.6933394435462997	0.0	0.0	0.0	0.0
10	0.9258669925446915	0.0	0.0	0.0	0.0
11	1.2933284112173327	0.0	0.0	0.0	0.0
12	1.4269893198569192	0.0	0.0	0.0	0.0
13	1.517369553317973	0.0	0.0	0.0	0.0
14	1.6684275960979502	0.0	0.0	0.0	0.0
15	1.8513096647444955	0.0	0.0	0.0	0.0
16	1.990911058212508	0.0	0.0	0.0	0.0
17	2.091899300295751	0.0	0.0	0.0	0.0
18	2.1996766678971955	0.0	0.0	0.0	0.0
19	2.3108485982577407	0.0	0.0	0.0	0.0
20	2.425839411722274	0.0	0.0	0.0	0.0
21	2.5306465369095053	0.0	0.0	0.0	0.0
22	2.649031913133139	0.0	0.0	0.0	0.0
23	2.8017872372926664	0.0	0.0	0.0	0.0
24	2.957512803866407	0.0	0.0	0.0	0.0
25	3.1094194873361594	0.0	0.0	0.0	0.0
26	3.3037582052946695	0.0	0.0	0.0	0.0
27	3.501067165667392	0.0	0.0	0.0	0.0
28	3.690314039487251	0.0	0.0	0.0	0.0
29	3.826096549851276	0.0	0.0	0.0	0.0
30	3.983095077459679	0.0	0.0	0.0	0.0
31	4.109542540236177	0.0	0.0	0.0	0.0
32	4.247870972669527	0.0	0.0	0.0	0.0
33	4.3785616388949	0.0	0.0	0.0	0.0
34	4.508403664430499	0.0	0.0	0.0	0.0
35	4.688315490662831	0.0	0.0	0.0	0.0
36	4.810095429645565	0.0	0.0	0.0	0.0
37	4.9267835244896485	0.0	0.0	0.0	0.0
38	5.054928268645697	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAAA	65	0.0	44.000004	31
GCCGTTA	65	0.0	44.000004	29
CCGTTAA	65	0.0	44.000004	30
AGGTCAC	25	4.4320408E-5	44.0	32
TCACGCG	25	4.4320408E-5	44.0	14
ACAACTA	25	4.4320408E-5	44.0	15
TTTAGTA	20	7.8437326E-4	44.0	16
GTGTTGT	20	7.8437326E-4	44.0	43
TCGCCCG	45	4.765752E-10	44.0	35
AACGTTT	35	1.43953E-7	44.0	17
CGGGTTT	20	7.8437326E-4	44.0	21
CTAGATA	25	4.4320408E-5	44.0	19
AGCGTTT	20	7.8437326E-4	44.0	26
GTCATAA	20	7.8437326E-4	44.0	11
AAACTCT	25	4.4320408E-5	44.0	21
CTAGACA	20	7.8437326E-4	44.0	34
CGGGTAC	35	1.43953E-7	44.0	40
AGCGTCG	20	7.8437326E-4	44.0	40
AAGTACT	25	4.4320408E-5	44.0	31
AACAAGG	20	7.8437326E-4	44.0	33
>>END_MODULE