SRR1527719_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527719_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	344638
Sequences flagged as poor quality	0
Sequence length	52
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTGATCAGGGAAG	2574	0.7468706294720837	No Hit
TGATCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1830	0.5309919393682647	No Hit
TGATCAGGGGAAGGCAGTTTTAACTTTTTCAAAGATTGCAAACTTCTTGTAG	1830	0.5309919393682647	No Hit
TGATCAGGGGAGTGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAAAAAA	1474	0.4276951467917061	No Hit
TGATCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1321	0.38330073874616266	No Hit
TGATCAGGGAGTGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAAAAAAA	1187	0.34441936176509846	No Hit
TGATCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1091	0.3165640469129928	No Hit
TGATCAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	1073	0.3113411753782229	No Hit
TGATCAGGGGAGTGCAGTGCTTGATCAGGGAAAAAAAAAAAAAAAAAAAAAA	970	0.2814547438181512	No Hit
TGATCAGGGGAGTGCAGTGCTTAATCAGGGGAAAAAAAAAAAAAAAAAAAAA	806	0.233868580945804	No Hit
TGATCAGGGGCCCGTCTGCCCCAGCCCAGACACCGAAGCTACTCTCCTTCCA	711	0.20630342562340775	No Hit
TGATCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	672	0.1949872039647398	No Hit
TGATCAGGGCAGAGTGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAAAA	641	0.18599225854374735	No Hit
TGATCAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC	632	0.18338082277636245	No Hit
TGATCAGGGGTGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAAAAAAAA	623	0.18076938700897754	No Hit
TGATCAGGGGGCGCCAGTCCTCCGATAGACTGAGTCGCCCGGGTACCCGTGT	569	0.16510077240466808	No Hit
TGATCAGGGAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTT	568	0.16481061287495866	No Hit
TGATCAGGGGAGTCCAGAGCCTGCAACCTACTTCCTTCTAGCTGCAGCCACT	566	0.16423029381553977	No Hit
TGATCAGGGAGTGCAGTGCTTAATCAGGGGAAAAAAAAAAAAAAAAAAAAAA	563	0.16335981522641146	No Hit
TGATCAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT	543	0.1575566246322228	No Hit
TGATCAGGGGACACCACCCACCCAGAGTGGAGAAGCCCAGCCAGTCGCTGTC	473	0.1372454575525624	No Hit
TGATCAGGGGCAGTGCATCGTGGGTGAGAACGAGCGGAGCAGGTTTGGCGTT	467	0.1355045003743058	No Hit
TGATCAGGGATTAAGAGCCTTGTCTGTTTAGTGCTGTGGATGGCTCCCAGAT	459	0.13318322413663033	No Hit
TGATCAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	451	0.13086194789895486	Illumina Single End Adapter 2 (100% over 34bp)
TGATCAGGGGAGTGCAGTGCTTAATCAGGGGGAAAAAAAAAAAAAAAAAAAA	447	0.1297013097801171	No Hit
TGATCAGGGGATCGAGCATCGGTCGGAGCTGCTGCCAGCCAACTCTCGAAGC	445	0.12912099072069824	No Hit
TGATCAGGGAGAGTGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAAAAA	436	0.12650955495331334	No Hit
TGATCAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	435	0.1262193954236039	No Hit
TGATCAGGGGGTACAGTGAAACTGCAAATGGCTGAGGACATTCTGTATCCCG	427	0.12389811918592843	No Hit
TGATCAGGGATTAGTTTGTGACTCTCTTGAGGGCAAGGGTGAGGTAGGGTCA	424	0.12302764059680012	No Hit
TGATCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA	420	0.1218670024779624	Illumina Single End Adapter 2 (100% over 34bp)
TGATCAGGGAGAGTGCAGTGCTTGATCAGGGAAGTAATTTACACCGGAAACA	419	0.12157684294825295	No Hit
TGATCAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	404	0.11722445000261143	Illumina Single End Adapter 2 (100% over 34bp)
TGATCAGGGAGTGCAGTGCTTGATCAGGGAAAAAAAAAAAAAAAAAAAAAAA	400	0.11606381188377371	No Hit
TGATCAGGGGTGCAGTGCTTAATCAGGGGAAAAAAAAAAAAAAAAAAAAAAA	390	0.11316221658667935	No Hit
TGATCAGGGGAGTGCAGTGCTTGATCGGAAGAGCTCGTATGCCGTCTTCTGC	388	0.11258189752726049	Illumina Single End Adapter 2 (96% over 31bp)
TGATCAGGGAACTTCACGCGTACACACAATACGTTTTGGCCCTCTCCACCTC	386	0.11200157846784162	No Hit
TGATCAGGGAGCAGACCGTAACCATTATAGACGCTATCCACGTCGTAGGGGT	384	0.11142125940842273	No Hit
TGATCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA	383	0.11113109987871332	Illumina Single End Adapter 2 (97% over 34bp)
TGATCAGGGCAGAGTGCAGTGCTTAATCAGGGGAAAAAAAAAAAAAAAAAAA	368	0.1067787069330718	No Hit
TGATCAGGGGAGACAGAGGACTGGCCATTCAAGGTCACTGTCAGACCCTGAG	360	0.10445743069539633	No Hit
TGATCAGGGGGCCCGTCCGCCCCAGCCCAGACACCGAAGCTACTCTCCTTCC	353	0.1024263139874303	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGTT	20	6.3052727E-4	46.000004	14
AGACTTC	20	6.3052727E-4	46.000004	38
AGGTATT	20	6.3052727E-4	46.000004	40
CGGGTAC	55	1.8189894E-12	46.000004	40
AACCGGT	40	5.5915734E-9	46.000004	23
GCCCATT	20	6.3052727E-4	46.000004	13
ACAAATC	20	6.3052727E-4	46.000004	46
ACCGGTA	40	5.5915734E-9	46.000004	24
TCCATAG	20	6.3052727E-4	46.000004	25
GTACCCG	55	1.8189894E-12	46.000004	43
AAATCGC	35	1.0167787E-7	46.000004	44
GATGTCT	20	6.3052727E-4	46.000004	14
CATATAA	20	6.3052727E-4	46.000004	32
GATCGCA	20	6.3052727E-4	46.000004	36
GTACACG	20	6.3052727E-4	46.000004	38
GGTCGGT	20	6.3052727E-4	46.000004	24
AAGAGCG	20	6.3052727E-4	46.000004	10
ACGCATG	20	6.3052727E-4	46.000004	24
CGCGGAA	20	6.3052727E-4	46.000004	23
AACCCTT	20	6.3052727E-4	46.000004	11

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content