##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527719_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	344638
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02680783894986	33.0	31.0	34.0	30.0	34.0
2	32.17636476534799	34.0	31.0	34.0	30.0	34.0
3	32.23062169580835	34.0	31.0	34.0	30.0	34.0
4	36.035376249862175	37.0	35.0	37.0	35.0	37.0
5	35.94523819195794	37.0	35.0	37.0	35.0	37.0
6	36.022243629547525	37.0	35.0	37.0	35.0	37.0
7	36.45974616844341	37.0	36.0	37.0	35.0	37.0
8	36.56495221072546	37.0	37.0	37.0	35.0	37.0
9	38.553598268327924	39.0	39.0	39.0	37.0	39.0
10	37.78918749528491	39.0	38.0	39.0	35.0	39.0
11	37.43527411370771	39.0	37.0	39.0	35.0	39.0
12	37.246551453989404	39.0	37.0	39.0	34.0	39.0
13	37.008185400333105	39.0	37.0	39.0	33.0	39.0
14	37.819210301823944	40.0	38.0	40.0	33.0	40.0
15	37.88153076561493	40.0	38.0	40.0	33.0	40.0
16	37.89026166586389	40.0	38.0	40.0	33.0	40.0
17	37.98738676524353	40.0	38.0	40.0	34.0	40.0
18	37.93987894544421	40.0	38.0	40.0	34.0	40.0
19	37.916848983571164	40.0	38.0	40.0	34.0	40.0
20	38.06276150627615	40.0	38.0	40.0	34.0	40.0
21	38.03987662416797	40.0	38.0	40.0	34.0	40.0
22	38.07622490845467	40.0	38.0	40.0	34.0	40.0
23	37.969855326458486	40.0	38.0	40.0	34.0	40.0
24	37.804966370510506	40.0	38.0	40.0	34.0	40.0
25	37.63907056099443	40.0	37.0	40.0	33.0	40.0
26	37.288322819886375	40.0	36.0	40.0	33.0	40.0
27	37.15995624394292	40.0	35.0	40.0	33.0	40.0
28	37.06127008629345	40.0	35.0	40.0	32.0	40.0
29	36.97744009656509	40.0	35.0	40.0	32.0	40.0
30	36.71532738699737	39.0	35.0	40.0	31.0	40.0
31	36.627078267631546	39.0	35.0	40.0	31.0	40.0
32	36.39732995200762	39.0	35.0	40.0	30.0	40.0
33	36.26823507564459	39.0	35.0	40.0	30.0	40.0
34	36.04919944985753	39.0	35.0	40.0	30.0	40.0
35	35.86694154446114	39.0	35.0	40.0	29.0	40.0
36	35.638379981313726	39.0	35.0	40.0	27.0	40.0
37	35.22656526558302	39.0	35.0	40.0	24.0	40.0
38	35.03928179713206	39.0	35.0	40.0	24.0	40.0
39	34.760322425269415	39.0	35.0	40.0	22.0	40.0
40	34.34904450466867	38.0	34.0	40.0	19.0	40.0
41	34.09772572960614	38.0	34.0	40.0	18.0	40.0
42	33.78990709091859	37.0	33.0	40.0	17.0	40.0
43	33.49786442586134	37.0	33.0	40.0	15.0	40.0
44	33.22820176533058	37.0	33.0	40.0	15.0	40.0
45	33.00715243240734	36.0	33.0	40.0	13.0	40.0
46	32.85131645378629	35.0	33.0	40.0	12.0	40.0
47	32.5999657611755	35.0	32.0	40.0	11.0	40.0
48	32.33668951189364	35.0	32.0	40.0	10.0	40.0
49	32.26058356884615	35.0	32.0	40.0	10.0	40.0
50	31.97366802267887	35.0	31.0	40.0	10.0	40.0
51	31.829040906690498	35.0	31.0	40.0	10.0	40.0
52	31.23730697137286	35.0	30.0	39.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	9.0
16	21.0
17	55.0
18	126.0
19	213.0
20	400.0
21	702.0
22	1147.0
23	1944.0
24	2952.0
25	4033.0
26	5557.0
27	7250.0
28	8689.0
29	8675.0
30	8174.0
31	8537.0
32	10403.0
33	13204.0
34	18435.0
35	23899.0
36	36196.0
37	57043.0
38	87780.0
39	39193.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	100.0	0.0	0.0
7	99.29955489528143	0.5695831568196195	0.101555835398302	0.029306112500652858
8	97.32269801937105	2.008774424178413	0.463094609416257	0.20543294703427945
9	94.78322181535408	3.322326615173022	1.1055078081929446	0.7889437612799517
10	47.4503682124432	37.13548709080252	5.55423371769799	9.859910979056286
11	28.639035741850872	36.9286033461197	18.190100917484433	16.242259994544998
12	37.078905982509184	24.873345365281832	20.905413796505318	17.142334855703666
13	24.55765179695797	27.98646695953435	24.57564168779995	22.88023955570773
14	27.759272047771866	31.367986118768098	20.743214619397747	20.129527214062293
15	25.785026607628875	32.27096257522386	20.952129480788535	20.99188133635873
16	26.436144592296845	31.298928150697254	25.46149873200286	16.803428525003046
17	30.64461841120248	29.357760896941137	20.605098683256053	19.39252200860033
18	28.56330410459671	29.826658696951586	23.337821134059507	18.272216064392204
19	25.949837220503834	28.873774801385803	24.98737806045764	20.189009917652726
20	25.982335087831288	28.83286230769677	23.977913056598517	21.20688954787342
21	22.79696377068112	27.51176596892972	29.2242875132748	20.46698274711436
22	22.942333695065546	31.94163150900365	23.477678027379454	21.63835676855135
23	22.024268943064897	35.67540433730465	23.249322477498126	19.051004242132326
24	21.48486237733506	34.65636406896512	24.373400495592477	19.485373058107346
25	22.51463854827384	31.155879502550505	22.997464005710338	23.332017943465317
26	25.961443601692206	31.942211828063073	20.40837052211306	21.687974048131665
27	23.924233543602273	32.84170637016231	21.04846244465207	22.185597641583342
28	29.335708772683223	31.507843012088045	19.654245904398238	19.502202310830494
29	31.16139253361498	32.236143431658725	19.120932688792298	17.481531345933995
30	29.15610002379308	34.57947179359211	19.554431026178193	16.70999715643661
31	28.636424306083484	33.38488500977837	18.90824575351528	19.070444930622855
32	24.79123021837406	36.502649156506244	20.578404006522785	18.127716618596903
33	27.422686993308922	36.4704414487085	19.246861924686193	16.860009633296386
34	24.390229748315623	37.74975481519739	17.58076590509462	20.27924953139236
35	23.682240495824605	42.26927964995154	18.33953307528479	15.708946778939062
36	24.696638211688786	39.49796598169674	18.59168170660229	17.213714100012186
37	22.95510071437276	40.79033652702256	18.93987314225361	17.31468961635107
38	25.14174293026306	39.831649440862584	18.396404343107843	16.630203285766516
39	23.363355172673934	37.669960944527304	18.106244813398405	20.860439069400353
40	23.045050168582687	39.775938811158376	19.895658633116486	17.28335238714245
41	22.65246432488582	40.09076190089311	20.384287281147177	16.872486493073893
42	23.108304946059345	39.87865528467551	19.082341471340943	17.9306982979242
43	22.031813090837343	40.24976932317388	20.296368943645216	17.42204864234356
44	22.013823199995358	38.261886385134545	21.425379673744622	18.29891074112547
45	19.962975644009077	39.00411446213128	19.687324090785115	21.345585803074528
46	21.05223451853829	40.64989931464319	19.456647264666113	18.841218902152406
47	23.337821134059507	38.651860793064024	19.61652516553601	18.393792907340455
48	23.818905634317748	37.69549498314173	19.528026508974634	18.957572873565887
49	23.18171530707583	38.43685258154934	20.26329075725834	18.118141354116492
50	20.487874233253443	40.93048357987221	20.35527132817623	18.22637085869811
51	20.53952262954172	40.391077014142375	20.030582814431376	19.03881754188453
52	22.06169952239741	37.679536209007715	20.374131697607346	19.884632570987527
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	15.0
4	30.0
5	53.5
6	77.0
7	93.0
8	109.0
9	881.5
10	1654.0
11	2231.5
12	2809.0
13	2672.0
14	2125.0
15	1715.0
16	1599.0
17	1483.0
18	1398.0
19	1313.0
20	1613.0
21	1913.0
22	2515.5
23	3118.0
24	4182.0
25	5246.0
26	5946.0
27	6646.0
28	8533.0
29	10420.0
30	11969.5
31	13519.0
32	14358.0
33	15197.0
34	14488.5
35	13780.0
36	14622.5
37	15465.0
38	14195.0
39	12888.5
40	12852.0
41	13385.5
42	13919.0
43	13628.0
44	13337.0
45	13804.0
46	14271.0
47	15305.5
48	16340.0
49	16723.0
50	17106.0
51	20701.5
52	24297.0
53	25157.5
54	26018.0
55	24251.0
56	22484.0
57	20292.5
58	18101.0
59	16749.5
60	15398.0
61	14004.5
62	12611.0
63	10835.0
64	7364.0
65	5669.0
66	5584.0
67	5499.0
68	4383.0
69	3267.0
70	2659.5
71	2052.0
72	1599.5
73	1147.0
74	1077.5
75	1008.0
76	541.5
77	75.0
78	95.0
79	115.0
80	84.5
81	54.0
82	29.5
83	5.0
84	2.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	344638.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.67135371026991
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.91875511188474	21.56117433364864
2	8.958119245852819	4.957665724615393
3	3.7151605393955913	3.084105641281577
4	1.9996644506427865	2.2133368926235644
5	1.280330516116855	1.771423928876096
6	0.8000754986053731	1.3283503270097898
7	0.5945515173122496	1.1516431734167445
8	0.4383113478598243	0.9702934673483481
9	0.3680557011932974	0.9166139543521027
>10	2.534446238701424	15.11992293362891
>50	0.8734769204957742	17.202688037883227
>100	0.4980810771134367	23.84879206587782
>500	0.012583100895497348	2.281814541634991
>1k	0.008388733930331564	3.5921749778027956
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTGATCAGGGAAG	2574	0.7468706294720837	No Hit
TGATCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1830	0.5309919393682647	No Hit
TGATCAGGGGAAGGCAGTTTTAACTTTTTCAAAGATTGCAAACTTCTTGTAG	1830	0.5309919393682647	No Hit
TGATCAGGGGAGTGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAAAAAA	1474	0.4276951467917061	No Hit
TGATCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1321	0.38330073874616266	No Hit
TGATCAGGGAGTGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAAAAAAA	1187	0.34441936176509846	No Hit
TGATCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1091	0.3165640469129928	No Hit
TGATCAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	1073	0.3113411753782229	No Hit
TGATCAGGGGAGTGCAGTGCTTGATCAGGGAAAAAAAAAAAAAAAAAAAAAA	970	0.2814547438181512	No Hit
TGATCAGGGGAGTGCAGTGCTTAATCAGGGGAAAAAAAAAAAAAAAAAAAAA	806	0.233868580945804	No Hit
TGATCAGGGGCCCGTCTGCCCCAGCCCAGACACCGAAGCTACTCTCCTTCCA	711	0.20630342562340775	No Hit
TGATCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	672	0.1949872039647398	No Hit
TGATCAGGGCAGAGTGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAAAA	641	0.18599225854374735	No Hit
TGATCAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC	632	0.18338082277636245	No Hit
TGATCAGGGGTGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAAAAAAAA	623	0.18076938700897754	No Hit
TGATCAGGGGGCGCCAGTCCTCCGATAGACTGAGTCGCCCGGGTACCCGTGT	569	0.16510077240466808	No Hit
TGATCAGGGAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTT	568	0.16481061287495866	No Hit
TGATCAGGGGAGTCCAGAGCCTGCAACCTACTTCCTTCTAGCTGCAGCCACT	566	0.16423029381553977	No Hit
TGATCAGGGAGTGCAGTGCTTAATCAGGGGAAAAAAAAAAAAAAAAAAAAAA	563	0.16335981522641146	No Hit
TGATCAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT	543	0.1575566246322228	No Hit
TGATCAGGGGACACCACCCACCCAGAGTGGAGAAGCCCAGCCAGTCGCTGTC	473	0.1372454575525624	No Hit
TGATCAGGGGCAGTGCATCGTGGGTGAGAACGAGCGGAGCAGGTTTGGCGTT	467	0.1355045003743058	No Hit
TGATCAGGGATTAAGAGCCTTGTCTGTTTAGTGCTGTGGATGGCTCCCAGAT	459	0.13318322413663033	No Hit
TGATCAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	451	0.13086194789895486	Illumina Single End Adapter 2 (100% over 34bp)
TGATCAGGGGAGTGCAGTGCTTAATCAGGGGGAAAAAAAAAAAAAAAAAAAA	447	0.1297013097801171	No Hit
TGATCAGGGGATCGAGCATCGGTCGGAGCTGCTGCCAGCCAACTCTCGAAGC	445	0.12912099072069824	No Hit
TGATCAGGGAGAGTGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAAAAA	436	0.12650955495331334	No Hit
TGATCAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	435	0.1262193954236039	No Hit
TGATCAGGGGGTACAGTGAAACTGCAAATGGCTGAGGACATTCTGTATCCCG	427	0.12389811918592843	No Hit
TGATCAGGGATTAGTTTGTGACTCTCTTGAGGGCAAGGGTGAGGTAGGGTCA	424	0.12302764059680012	No Hit
TGATCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA	420	0.1218670024779624	Illumina Single End Adapter 2 (100% over 34bp)
TGATCAGGGAGAGTGCAGTGCTTGATCAGGGAAGTAATTTACACCGGAAACA	419	0.12157684294825295	No Hit
TGATCAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	404	0.11722445000261143	Illumina Single End Adapter 2 (100% over 34bp)
TGATCAGGGAGTGCAGTGCTTGATCAGGGAAAAAAAAAAAAAAAAAAAAAAA	400	0.11606381188377371	No Hit
TGATCAGGGGTGCAGTGCTTAATCAGGGGAAAAAAAAAAAAAAAAAAAAAAA	390	0.11316221658667935	No Hit
TGATCAGGGGAGTGCAGTGCTTGATCGGAAGAGCTCGTATGCCGTCTTCTGC	388	0.11258189752726049	Illumina Single End Adapter 2 (96% over 31bp)
TGATCAGGGAACTTCACGCGTACACACAATACGTTTTGGCCCTCTCCACCTC	386	0.11200157846784162	No Hit
TGATCAGGGAGCAGACCGTAACCATTATAGACGCTATCCACGTCGTAGGGGT	384	0.11142125940842273	No Hit
TGATCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA	383	0.11113109987871332	Illumina Single End Adapter 2 (97% over 34bp)
TGATCAGGGCAGAGTGCAGTGCTTAATCAGGGGAAAAAAAAAAAAAAAAAAA	368	0.1067787069330718	No Hit
TGATCAGGGGAGACAGAGGACTGGCCATTCAAGGTCACTGTCAGACCCTGAG	360	0.10445743069539633	No Hit
TGATCAGGGGGCCCGTCCGCCCCAGCCCAGACACCGAAGCTACTCTCCTTCC	353	0.1024263139874303	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.39867919382076267	0.0	0.0	0.0	0.0
7	0.4770222668423099	0.0	0.0	0.0	0.0
8	0.5228674725364005	0.0	0.0	0.0	0.0
9	0.60004990743911	0.0	0.0	0.0	0.0
10	0.8040320568248422	0.0	0.0	0.0	0.0
11	1.1281402515102803	0.0	0.0	0.0	0.0
12	1.248266296809986	0.0	0.0	0.0	0.0
13	1.3254487317126957	0.0	0.0	0.0	0.0
14	1.4708186560971221	0.0	0.0	0.0	0.0
15	1.6547797979329035	0.0	0.0	0.0	0.0
16	1.7833204695941829	0.0	0.0	0.0	0.0
17	1.8982236433591189	0.0	0.0	0.0	0.0
18	2.003261393113934	0.0	0.0	0.0	0.0
19	2.1045270689825264	0.0	0.0	0.0	0.0
20	2.2200105618068813	0.0	0.0	0.0	0.0
21	2.3189549614377984	0.0	0.0	0.0	0.0
22	2.4440137187425646	0.0	0.0	0.0	0.0
23	2.5809690167654176	0.0	0.0	0.0	0.0
24	2.7144424004317576	0.0	0.0	0.0	0.0
25	2.842402753033618	0.0	0.0	0.0	0.0
26	3.0104051207353804	0.0	0.0	0.0	0.0
27	3.1749255740806297	0.0	0.0	0.0	0.0
28	3.343218101312101	0.0	0.0	0.0	0.0
29	3.46189334896326	0.0	0.0	0.0	0.0
30	3.5982683279266943	0.0	0.0	0.0	0.0
31	3.709979746864826	0.0	0.0	0.0	0.0
32	3.840261375704362	0.0	0.0	0.0	0.0
33	3.9571956661772645	0.0	0.0	0.0	0.0
34	4.070648042293653	0.0	0.0	0.0	0.0
35	4.229655464574423	0.0	0.0	0.0	0.0
36	4.34949135034442	0.0	0.0	0.0	0.0
37	4.468166597995578	0.0	0.0	0.0	0.0
38	4.590323760003249	0.0	0.0	0.0	0.0
39	4.722056186491333	0.0	0.0	0.0	0.0
40	4.853788612979416	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGTT	20	6.3052727E-4	46.000004	14
AGACTTC	20	6.3052727E-4	46.000004	38
AGGTATT	20	6.3052727E-4	46.000004	40
CGGGTAC	55	1.8189894E-12	46.000004	40
AACCGGT	40	5.5915734E-9	46.000004	23
GCCCATT	20	6.3052727E-4	46.000004	13
ACAAATC	20	6.3052727E-4	46.000004	46
ACCGGTA	40	5.5915734E-9	46.000004	24
TCCATAG	20	6.3052727E-4	46.000004	25
GTACCCG	55	1.8189894E-12	46.000004	43
AAATCGC	35	1.0167787E-7	46.000004	44
GATGTCT	20	6.3052727E-4	46.000004	14
CATATAA	20	6.3052727E-4	46.000004	32
GATCGCA	20	6.3052727E-4	46.000004	36
GTACACG	20	6.3052727E-4	46.000004	38
GGTCGGT	20	6.3052727E-4	46.000004	24
AAGAGCG	20	6.3052727E-4	46.000004	10
ACGCATG	20	6.3052727E-4	46.000004	24
CGCGGAA	20	6.3052727E-4	46.000004	23
AACCCTT	20	6.3052727E-4	46.000004	11
>>END_MODULE