FastQCFastQC Report
Fri 17 Jun 2016
SRR1527718_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527718_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347589
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTGATCAGGGAAG25150.723555693649684No Hit
TGATCAGGGGAAGGCAGTTTTAACTTTTTCAAAGATTGCAAACTTCTTGTAG17520.5040435686975134No Hit
TGATCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16960.4879325870496477No Hit
TGATCAGGGGAGTGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAAAAAA13790.3967329230786935No Hit
TGATCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11310.32538429006671676No Hit
TGATCAGGGAGTGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAAAAAAA11280.3245212017641525No Hit
TGATCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11200.32221963295731454No Hit
TGATCAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA10600.30495786690602983No Hit
TGATCAGGGGAGTGCAGTGCTTGATCAGGGAAAAAAAAAAAAAAAAAAAAAA8510.2448293818273881No Hit
TGATCAGGGGAGTGCAGTGCTTAATCAGGGGAAAAAAAAAAAAAAAAAAAAA7680.22095060545644424No Hit
TGATCAGGGGCCCGTCTGCCCCAGCCCAGACACCGAAGCTACTCTCCTTCCA6480.18642707335387482No Hit
TGATCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6200.17837158252994198No Hit
TGATCAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC5940.1708914839077186No Hit
TGATCAGGGCAGAGTGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAAAA5930.17060378780686386No Hit
TGATCAGGGGAGTCCAGAGCCTGCAACCTACTTCCTTCTAGCTGCAGCCACT5870.1688776112017354No Hit
TGATCAGGGGTGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAAAAAAAA5690.16369908138634998No Hit
TGATCAGGGAGTGCAGTGCTTAATCAGGGGAAAAAAAAAAAAAAAAAAAAAA5610.16139751257951201No Hit
TGATCAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA5120.1473004036376295Illumina Single End Adapter 2 (100% over 34bp)
TGATCAGGGGGCGCCAGTCCTCCGATAGACTGAGTCGCCCGGGTACCCGTGT5090.14643731533506527No Hit
TGATCAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT5080.14614961923421052No Hit
TGATCAGGGAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTT4840.13924491281369664No Hit
TGATCAGGGGAGTGCAGTGCTTAATCAGGGGGAAAAAAAAAAAAAAAAAAAA4700.1352171674017302No Hit
TGATCAGGGGACACCACCCACCCAGAGTGGAGAAGCCCAGCCAGTCGCTGTC4640.13349099079660173No Hit
TGATCAGGGGCAGTGCATCGTGGGTGAGAACGAGCGGAGCAGGTTTGGCGTT4540.13061402978805428No Hit
TGATCAGGGATTAAGAGCCTTGTCTGTTTAGTGCTGTGGATGGCTCCCAGAT4520.1300386375863448No Hit
TGATCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4470.12860015708207106Illumina Single End Adapter 2 (100% over 34bp)
TGATCAGGGGATCGAGCATCGGTCGGAGCTGCTGCCAGCCAACTCTCGAAGC4230.1216954506615572No Hit
TGATCAGGGAGAGTGCAGTGCTTGATCAGGGAAGTAATTTACACCGGAAACA4200.12083236235899296No Hit
TGATCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA4200.12083236235899296Illumina Single End Adapter 2 (97% over 34bp)
TGATCAGGGAGAGTGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAAAAA4100.1179554013504455No Hit
TGATCAGGGAGTGCAGTGCTTGATCAGGGAAAAAAAAAAAAAAAAAAAAAAA4100.1179554013504455No Hit
TGATCAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA4030.11594152864446228Illumina Single End Adapter 2 (100% over 34bp)
TGATCAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG3890.11191378323249586No Hit
TGATCAGGGGAGTGCAGTGCTTGATCGGAAGAGCTCGTATGCCGTCTTCTGC3800.10932451832480314Illumina Single End Adapter 2 (96% over 31bp)
TGATCAGGGAGCAGACCGTAACCATTATAGACGCTATCCACGTCGTAGGGGT3800.10932451832480314No Hit
TGATCAGGGGTGCAGTGCTTAATCAGGGGAAAAAAAAAAAAAAAAAAAAAAA3760.10817373392138416No Hit
TGATCAGGGATTAGTTTGTGACTCTCTTGAGGGCAAGGGTGAGGTAGGGTCA3710.10673525341711045No Hit
TGATCAGGGGGTACAGTGAAACTGCAAATGGCTGAGGACATTCTGTATCCCG3680.10587216511454621No Hit
TGATCAGGGGAGACAGAGGACTGGCCATTCAAGGTCACTGTCAGACCCTGAG3660.10529677291283672No Hit
TGATCAGGGCAGAGTGCAGTGCTTAATCAGGGGAAAAAAAAAAAAAAAAAAA3550.10213211580343451No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACAAC253.412203E-546.013
TCACGCG206.3053484E-446.014
TCACGAC301.8576302E-646.042
TCTGCGA206.3053484E-446.015
CTTGTAG2400.046.046
TCGCCCG650.046.035
TCGCCCC501.6370905E-1146.043
ATTTACG206.3053484E-446.023
CGGGTTA206.3053484E-446.041
ATCCTCT253.412203E-546.037
AGGTATT301.8576302E-646.040
CTATGGG405.5915734E-946.035
CGGGTAC650.046.040
AGCGTAT206.3053484E-446.038
CGTGGAA301.8576302E-646.031
AGACTAG253.412203E-546.033
CGGCGGA253.412203E-546.029
GATACCG253.412203E-546.019
GTCGCGA253.412203E-546.020
TCGTTAT206.3053484E-446.042
Produced by FastQC (version 0.11.5)