##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527718_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	347589
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.978324975761602	33.0	31.0	34.0	30.0	34.0
2	32.14906110377486	34.0	31.0	34.0	30.0	34.0
3	32.21922442885131	34.0	31.0	34.0	30.0	34.0
4	36.028110786014516	37.0	35.0	37.0	35.0	37.0
5	35.95668735201632	37.0	35.0	37.0	35.0	37.0
6	35.94606273501175	37.0	35.0	37.0	35.0	37.0
7	36.42272338882991	37.0	36.0	37.0	35.0	37.0
8	36.53967473078838	37.0	37.0	37.0	35.0	37.0
9	38.51408128565634	39.0	39.0	39.0	37.0	39.0
10	37.71173138390456	39.0	38.0	39.0	35.0	39.0
11	37.39384733118712	39.0	37.0	39.0	34.0	39.0
12	37.19302969886849	39.0	37.0	39.0	34.0	39.0
13	36.79677722827822	39.0	37.0	39.0	33.0	39.0
14	37.75272232435434	40.0	37.0	40.0	33.0	40.0
15	37.84000932135367	40.0	37.0	40.0	33.0	40.0
16	37.87482054955709	40.0	38.0	40.0	33.0	40.0
17	38.02276251549963	40.0	38.0	40.0	34.0	40.0
18	37.97396350287264	40.0	38.0	40.0	34.0	40.0
19	37.90715471433216	40.0	38.0	40.0	34.0	40.0
20	38.05734934074439	40.0	38.0	40.0	34.0	40.0
21	38.09829712677904	40.0	38.0	40.0	34.0	40.0
22	38.027411684489444	40.0	38.0	40.0	34.0	40.0
23	37.94082091205418	40.0	38.0	40.0	34.0	40.0
24	37.82350419604763	40.0	38.0	40.0	34.0	40.0
25	37.6206007669978	40.0	37.0	40.0	33.0	40.0
26	37.228073960913605	40.0	36.0	40.0	33.0	40.0
27	37.10539746654813	40.0	35.0	40.0	33.0	40.0
28	37.038418937308144	40.0	35.0	40.0	32.0	40.0
29	36.94406612407182	39.0	35.0	40.0	32.0	40.0
30	36.66529435626559	39.0	35.0	40.0	31.0	40.0
31	36.6096942078144	39.0	35.0	40.0	31.0	40.0
32	36.38096717675185	39.0	35.0	40.0	30.0	40.0
33	36.03309080552031	39.0	35.0	40.0	30.0	40.0
34	35.94362019511549	39.0	35.0	40.0	30.0	40.0
35	35.83521630431343	39.0	35.0	40.0	29.0	40.0
36	35.49077214756508	39.0	35.0	40.0	26.0	40.0
37	35.14936318468076	39.0	35.0	40.0	24.0	40.0
38	34.70416785341308	38.0	35.0	40.0	21.0	40.0
39	34.59164703140778	38.0	34.0	40.0	21.0	40.0
40	34.25675150824681	38.0	34.0	40.0	18.0	40.0
41	33.80456516172836	38.0	33.0	40.0	15.0	40.0
42	33.63869685174157	37.0	33.0	40.0	16.0	40.0
43	33.465236241653216	37.0	33.0	40.0	15.0	40.0
44	33.19281392679285	37.0	33.0	40.0	15.0	40.0
45	32.96746732491535	36.0	33.0	40.0	13.0	40.0
46	32.636642701581465	35.0	32.0	40.0	11.0	40.0
47	32.32772038240566	35.0	32.0	40.0	10.0	40.0
48	32.2176018228425	35.0	31.0	39.0	10.0	40.0
49	31.995310553556067	35.0	31.0	39.0	10.0	40.0
50	31.534890344631158	35.0	30.0	39.0	10.0	40.0
51	31.516161328465515	35.0	30.0	39.0	9.0	40.0
52	31.073900497426557	35.0	29.0	39.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	1.0
14	1.0
15	5.0
16	20.0
17	53.0
18	113.0
19	244.0
20	403.0
21	761.0
22	1245.0
23	1986.0
24	2971.0
25	4152.0
26	5604.0
27	7435.0
28	8768.0
29	8924.0
30	8499.0
31	9053.0
32	10845.0
33	13536.0
34	19385.0
35	25103.0
36	37933.0
37	59370.0
38	86804.0
39	34373.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	100.0	0.0	0.0
7	99.27241656093835	0.5877631340462443	0.10299520410599874	0.036825100909407375
8	97.2504883641312	2.075727367666986	0.46923234049408924	0.2045519277077238
9	94.70725483257525	3.3835938421526572	1.1234532738377796	0.7856980514343088
10	47.50179090822782	36.98592302978518	5.607772397860692	9.904513664126311
11	28.52017756603345	37.04432533825869	18.22123254763528	16.21426454807258
12	37.28311310196813	24.786745265241418	21.139334098604962	16.79080753418549
13	24.854929241143996	27.99340600536841	24.500199948790094	22.6514648046975
14	27.755481329961533	31.369519748898846	20.790358728268153	20.08464019287147
15	25.7099620528843	32.34854958010754	20.946002318830573	20.99548604817759
16	26.508894124957926	31.220780864756943	25.517493361412473	16.75283164887266
17	30.736012934816692	29.34011145346947	20.555023317768974	19.36885229394486
18	28.495148005259086	30.017635770982398	23.364663438716416	18.122552785042103
19	25.998521242041605	28.882099260908717	25.062645825961123	20.056733671088555
20	26.012906047084343	28.885551614118977	23.995868683991723	21.105673654804956
21	22.898020363130016	27.39787507659909	29.269625908760062	20.434478651510837
22	23.07207650414714	31.782939045827113	23.4575892792925	21.68739517073325
23	22.10628069357776	35.68122121240891	23.163563864218947	19.048934229794384
24	21.51938064783408	34.59372995117797	24.371599791708025	19.515289609279925
25	22.697208484733405	30.974800698526135	23.028922089018927	23.299068727721533
26	26.26406474313054	31.904634496488672	20.351046782262962	21.480253978117837
27	23.95616662207377	32.824974323122994	21.099056644485298	22.11980241031793
28	29.365141014243832	31.48632436584587	19.71869075258423	19.429843867326067
29	31.056794087269736	32.19204290124256	19.114241244688408	17.636921766799293
30	29.14102575167799	34.679751085333535	19.455161124201283	16.72406203878719
31	28.553838009833456	33.27982185857435	19.001176677052495	19.165163454539698
32	24.82299497394912	36.36622562854406	20.639318275319415	18.17146112218741
33	27.389244193573443	36.40305072945346	19.342959644867932	16.864745432105156
34	24.423385089861878	37.67869524064341	17.648429610833485	20.249490058661234
35	23.59223105449252	42.12446308715178	18.583441938611404	15.699863919744296
36	24.98870792804145	39.21182776209834	18.496270020052417	17.30319428980779
37	22.92161144340011	40.68195483746609	19.008081383473012	17.388352335660795
38	25.31380452200731	39.52570420813087	18.264962354965203	16.895528914896616
39	23.242680291954006	37.83174956629813	17.97122463599251	20.954345505755363
40	23.200388965128354	39.71069280098047	19.923242680291953	17.165675553599222
41	22.922474531702672	39.740037803267654	20.574011260425387	16.763476404604287
42	23.071788808046286	39.91754629749503	19.068497564652507	17.942167329806182
43	22.202083495162388	40.232573527930974	20.376364039138178	17.188978937768457
44	22.18079398369914	38.30472195610333	21.52714844255716	17.987335617640372
45	20.11398519515865	38.898526708267525	19.59498142921669	21.392506667357136
46	21.436524170787912	40.554793160888295	19.35734444991067	18.651338218413127
47	23.596834192106193	38.445117653320445	19.678413298464566	18.279634856108796
48	23.718529642767752	37.594112586992104	19.684167220481662	19.00319054975848
49	23.32841373000872	38.31450362353239	20.147070246756947	18.210012399701945
50	20.78920794386474	41.037547218122555	20.199718633213365	17.97352620479935
51	20.54207699323051	40.39483412881305	20.068816907324454	18.99427197063198
52	22.14511966719315	37.57483694823484	20.36514389120484	19.914899493367166
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	17.0
4	34.0
5	68.0
6	102.0
7	124.0
8	146.0
9	915.5
10	1685.0
11	2211.5
12	2738.0
13	2560.5
14	2044.5
15	1706.0
16	1598.5
17	1491.0
18	1408.5
19	1326.0
20	1614.0
21	1902.0
22	2548.5
23	3195.0
24	4157.5
25	5120.0
26	5967.0
27	6814.0
28	8747.5
29	10681.0
30	12068.5
31	13456.0
32	14266.0
33	15076.0
34	14524.0
35	13972.0
36	14781.5
37	15591.0
38	14230.0
39	13019.5
40	13170.0
41	13647.5
42	14125.0
43	13901.0
44	13677.0
45	14069.5
46	14462.0
47	15513.0
48	16564.0
49	16865.5
50	17167.0
51	20764.0
52	24361.0
53	25317.0
54	26273.0
55	24416.5
56	22560.0
57	20469.0
58	18378.0
59	17043.5
60	15709.0
61	14111.5
62	12514.0
63	10943.5
64	7600.5
65	5828.0
66	5621.0
67	5414.0
68	4328.5
69	3243.0
70	2648.5
71	2054.0
72	1632.5
73	1211.0
74	1078.5
75	946.0
76	522.5
77	99.0
78	105.0
79	111.0
80	80.0
81	49.0
82	29.0
83	9.0
84	6.0
85	3.0
86	2.5
87	2.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	347589.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.4489730024392
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.50688389905483	23.11947104448894
2	9.087547335169374	5.352378722635824
3	3.596041814782627	3.1769921495752813
4	1.9115024567933625	2.2516713697681574
5	1.10105355559486	1.6212448216476363
6	0.7853192282137113	1.3876106849977183
7	0.5605137145606434	1.1554587273315309
8	0.41763764176892354	0.9839199709804323
9	0.34470092222111975	0.9135979337165083
>10	2.427893450541051	15.533812972667885
>50	0.7983073526610855	16.64278257320303
>100	0.4426159664941939	22.240887009632512
>500	0.01198959728652444	2.1537960934859566
>1k	0.00799306485768296	3.4663759258685864
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTGATCAGGGAAG	2515	0.723555693649684	No Hit
TGATCAGGGGAAGGCAGTTTTAACTTTTTCAAAGATTGCAAACTTCTTGTAG	1752	0.5040435686975134	No Hit
TGATCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1696	0.4879325870496477	No Hit
TGATCAGGGGAGTGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAAAAAA	1379	0.3967329230786935	No Hit
TGATCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1131	0.32538429006671676	No Hit
TGATCAGGGAGTGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAAAAAAA	1128	0.3245212017641525	No Hit
TGATCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1120	0.32221963295731454	No Hit
TGATCAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	1060	0.30495786690602983	No Hit
TGATCAGGGGAGTGCAGTGCTTGATCAGGGAAAAAAAAAAAAAAAAAAAAAA	851	0.2448293818273881	No Hit
TGATCAGGGGAGTGCAGTGCTTAATCAGGGGAAAAAAAAAAAAAAAAAAAAA	768	0.22095060545644424	No Hit
TGATCAGGGGCCCGTCTGCCCCAGCCCAGACACCGAAGCTACTCTCCTTCCA	648	0.18642707335387482	No Hit
TGATCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	620	0.17837158252994198	No Hit
TGATCAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC	594	0.1708914839077186	No Hit
TGATCAGGGCAGAGTGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAAAA	593	0.17060378780686386	No Hit
TGATCAGGGGAGTCCAGAGCCTGCAACCTACTTCCTTCTAGCTGCAGCCACT	587	0.1688776112017354	No Hit
TGATCAGGGGTGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAAAAAAAA	569	0.16369908138634998	No Hit
TGATCAGGGAGTGCAGTGCTTAATCAGGGGAAAAAAAAAAAAAAAAAAAAAA	561	0.16139751257951201	No Hit
TGATCAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	512	0.1473004036376295	Illumina Single End Adapter 2 (100% over 34bp)
TGATCAGGGGGCGCCAGTCCTCCGATAGACTGAGTCGCCCGGGTACCCGTGT	509	0.14643731533506527	No Hit
TGATCAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT	508	0.14614961923421052	No Hit
TGATCAGGGAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTT	484	0.13924491281369664	No Hit
TGATCAGGGGAGTGCAGTGCTTAATCAGGGGGAAAAAAAAAAAAAAAAAAAA	470	0.1352171674017302	No Hit
TGATCAGGGGACACCACCCACCCAGAGTGGAGAAGCCCAGCCAGTCGCTGTC	464	0.13349099079660173	No Hit
TGATCAGGGGCAGTGCATCGTGGGTGAGAACGAGCGGAGCAGGTTTGGCGTT	454	0.13061402978805428	No Hit
TGATCAGGGATTAAGAGCCTTGTCTGTTTAGTGCTGTGGATGGCTCCCAGAT	452	0.1300386375863448	No Hit
TGATCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA	447	0.12860015708207106	Illumina Single End Adapter 2 (100% over 34bp)
TGATCAGGGGATCGAGCATCGGTCGGAGCTGCTGCCAGCCAACTCTCGAAGC	423	0.1216954506615572	No Hit
TGATCAGGGAGAGTGCAGTGCTTGATCAGGGAAGTAATTTACACCGGAAACA	420	0.12083236235899296	No Hit
TGATCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA	420	0.12083236235899296	Illumina Single End Adapter 2 (97% over 34bp)
TGATCAGGGAGAGTGCAGTGCTTAATCAGGGAAAAAAAAAAAAAAAAAAAAA	410	0.1179554013504455	No Hit
TGATCAGGGAGTGCAGTGCTTGATCAGGGAAAAAAAAAAAAAAAAAAAAAAA	410	0.1179554013504455	No Hit
TGATCAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	403	0.11594152864446228	Illumina Single End Adapter 2 (100% over 34bp)
TGATCAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	389	0.11191378323249586	No Hit
TGATCAGGGGAGTGCAGTGCTTGATCGGAAGAGCTCGTATGCCGTCTTCTGC	380	0.10932451832480314	Illumina Single End Adapter 2 (96% over 31bp)
TGATCAGGGAGCAGACCGTAACCATTATAGACGCTATCCACGTCGTAGGGGT	380	0.10932451832480314	No Hit
TGATCAGGGGTGCAGTGCTTAATCAGGGGAAAAAAAAAAAAAAAAAAAAAAA	376	0.10817373392138416	No Hit
TGATCAGGGATTAGTTTGTGACTCTCTTGAGGGCAAGGGTGAGGTAGGGTCA	371	0.10673525341711045	No Hit
TGATCAGGGGGTACAGTGAAACTGCAAATGGCTGAGGACATTCTGTATCCCG	368	0.10587216511454621	No Hit
TGATCAGGGGAGACAGAGGACTGGCCATTCAAGGTCACTGTCAGACCCTGAG	366	0.10529677291283672	No Hit
TGATCAGGGCAGAGTGCAGTGCTTAATCAGGGGAAAAAAAAAAAAAAAAAAA	355	0.10213211580343451	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.4030622372974979	0.0	0.0	0.0	0.0
7	0.471821605401782	0.0	0.0	0.0	0.0
8	0.5161268049334128	0.0	0.0	0.0	0.0
9	0.5929416638616297	0.0	0.0	0.0	0.0
10	0.8207969757385878	0.0	0.0	0.0	0.0
11	1.164881512360863	0.0	0.0	0.0	0.0
12	1.2865769630224202	0.0	0.0	0.0	0.0
13	1.3619533414463634	0.0	0.0	0.0	0.0
14	1.4894027141250155	0.0	0.0	0.0	0.0
15	1.6562664526207675	0.0	0.0	0.0	0.0
16	1.7871681785096767	0.0	0.0	0.0	0.0
17	1.900808138347301	0.0	0.0	0.0	0.0
18	2.008981872268685	0.0	0.0	0.0	0.0
19	2.1148540373832314	0.0	0.0	0.0	0.0
20	2.227343212817437	0.0	0.0	0.0	0.0
21	2.3300507208225807	0.0	0.0	0.0	0.0
22	2.4577877896020874	0.0	0.0	0.0	0.0
23	2.5884018193901417	0.0	0.0	0.0	0.0
24	2.730523693212386	0.0	0.0	0.0	0.0
25	2.860562330798731	0.0	0.0	0.0	0.0
26	3.0167813135628574	0.0	0.0	0.0	0.0
27	3.1778911300415147	0.0	0.0	0.0	0.0
28	3.353673447663764	0.0	0.0	0.0	0.0
29	3.4644364464928405	0.0	0.0	0.0	0.0
30	3.6010920943988447	0.0	0.0	0.0	0.0
31	3.7003472491937317	0.0	0.0	0.0	0.0
32	3.826645837468965	0.0	0.0	0.0	0.0
33	3.9397104051048797	0.0	0.0	0.0	0.0
34	4.051336492236521	0.0	0.0	0.0	0.0
35	4.217624838530563	0.0	0.0	0.0	0.0
36	4.321483130939126	0.0	0.0	0.0	0.0
37	4.441452404995555	0.0	0.0	0.0	0.0
38	4.5605585907494195	0.0	0.0	0.0	0.0
39	4.688007963428071	0.0	0.0	0.0	0.0
40	4.817758904913561	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCACAAC	25	3.412203E-5	46.0	13
TCACGCG	20	6.3053484E-4	46.0	14
TCACGAC	30	1.8576302E-6	46.0	42
TCTGCGA	20	6.3053484E-4	46.0	15
CTTGTAG	240	0.0	46.0	46
TCGCCCG	65	0.0	46.0	35
TCGCCCC	50	1.6370905E-11	46.0	43
ATTTACG	20	6.3053484E-4	46.0	23
CGGGTTA	20	6.3053484E-4	46.0	41
ATCCTCT	25	3.412203E-5	46.0	37
AGGTATT	30	1.8576302E-6	46.0	40
CTATGGG	40	5.5915734E-9	46.0	35
CGGGTAC	65	0.0	46.0	40
AGCGTAT	20	6.3053484E-4	46.0	38
CGTGGAA	30	1.8576302E-6	46.0	31
AGACTAG	25	3.412203E-5	46.0	33
CGGCGGA	25	3.412203E-5	46.0	29
GATACCG	25	3.412203E-5	46.0	19
GTCGCGA	25	3.412203E-5	46.0	20
TCGTTAT	20	6.3053484E-4	46.0	42
>>END_MODULE