FastQCFastQC Report
Fri 17 Jun 2016
SRR1527717_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527717_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159815
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGA33772.113068235146889No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG10370.6488752620217126Illumina Single End Adapter 2 (100% over 23bp)
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAA9390.5875543597284361No Hit
GCGTCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9010.5637768670024715No Hit
GCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8270.5174733285361199No Hit
GCGTCCGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAA7640.4780527484904421No Hit
GCGTCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7150.44739229734380376No Hit
GCGTCCGGGAAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTC6200.3879485655288928Illumina Single End Adapter 2 (100% over 25bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGG6180.3866971185433157No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCG4920.3078559584519601No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCC4610.2884585301755155Illumina Single End Adapter 2 (95% over 22bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGC4180.26155241998560835Illumina Single End Adapter 2 (100% over 21bp)
GCGTCCGGGGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATCTC4160.26030097300003124No Hit
GCGTCCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA4070.2546694615649344Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCTTATGAATGTTTTGACCATTTTCATGGTGGAAGAATTTTATATT4040.25279229108656887No Hit
GCGTCCGGGACCTATGCTCCTCCTCTCCTTGCGTGGGGTACACCCCATCT3690.23089196883897006No Hit
GCGTCCGGGAGGAAGTTCCTGTGTGACATAGTGCTGGCACTGATGTTGTC3640.2277633513750274No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGGCCGGGA3460.21650032850483372No Hit
GCGTCCGGGAAGCAGTGGTATCAAGATCGGAAGAGCTCGTATGCCGTCTT3410.213371711040891Illumina Single End Adapter 2 (100% over 27bp)
GCGTCCGGGACCTTAACTCTTAAATAGGGTAATTTCAATGACCTAGACTC3330.20836592309858276No Hit
GCGTCCGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA3310.20711447611300565Illumina Single End Adapter 2 (100% over 34bp)
GCGTCCGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3280.20523730563464004Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3110.19460000625723492No Hit
GCGTCCGGGAGCACATGTGCCATCATGATGGATTTCTTTAAGTATGTGTA2760.17269968400963615No Hit
GCGTCCGGGAGGGTCAAAACCTACAAACCATCAACTAAAGCCTCCAAGCA2730.17082251353127054No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTC2640.1651910020961737No Hit
GCGTCCGGGATGAGAAGAAAGCTGCCAAGCTGAAGGAGAAGTATGAGAAG2630.16456527860338516No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGACCGGGA2600.16268810812501955No Hit
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGGAAAAAAAAAAAAAAAAAAA2600.16268810812501955No Hit
GCGTCCGGGATGTCTGCCCTTTCCCCACTTGCTCACACAAAAACAAATAT2490.15580514970434564No Hit
GCGTCCGGGGAAAACTTCACTGAGCGAAAGAGGCAGGGCCATGGCCTTAG2380.14892219128367173No Hit
GCGTCCGGGAAGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA2370.1482964677908832Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGATCCCAGACCCCCATAATAACAGGAGGGGCCTAGGGAGCCC2360.14767074429809468No Hit
GCGTCCGGGCAGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAA2320.14516785032694052No Hit
GCGTCCGGGATGGTGACTGCTGTAAATACTCTGCTGTCAAGAGCATAGTG2270.14203923286299783No Hit
GCGTCCGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC2230.1395363388918437No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCCGCGTGCCGGC2220.13891061539905517No Hit
GCGTCCGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA2140.13390482745674687Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGATCGGAAGAGCTCGTA2100.1314019334855927No Hit
GCGTCCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCG2100.1314019334855927No Hit
GCGTCCGGGAGTGGCTGATACTGGGGTTTACATGAACACAGAGTGTATTT2070.1295247630072271No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGT2050.12827331602165004Illumina Single End Adapter 2 (95% over 24bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGC2020.12639614554328443No Hit
GCGTCCGGGATTGCACCTCTGGACATTGGTGGTGCTGACCAAGAATTCCG2010.12577042205049588No Hit
GCGTCCGGGGGGACATCTTCACTGACCCTCCTCATCATTGGACATTAGCG2000.12514469855770735No Hit
GCGTCCGGGAAGGCAGGAGCGTCTCAGAGTTGAAGGCCAGAATGGACTAC1980.12389325157213028No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGGGCAGTGCCGCGTGCCGGC1980.12389325157213028No Hit
GCGTCCGGGGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAA1960.1226418045865532No Hit
GCGTCCGGGAAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTT1950.12201608109376468Illumina Single End Adapter 2 (96% over 33bp)
GCGTCCGGGAGTGCAGTGCTGCGTCCGGGGAAAAAAAAAAAAAAAAAAAA1930.12076463410818761No Hit
GCGTCCTGTCCCCTGCCTGATACTTGGTGTATGCAAGAGTATTGCCCTCC1920.12013891061539905No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGAGATCGGAAGAGCTCG1920.12013891061539905No Hit
GCGTCCGGGGAGAGGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCG1910.11951318712261053No Hit
GCGTCCGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGAAG1890.11826174013703344No Hit
GCGTCCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA1880.1176360166442449No Hit
GCGTCCGGGGGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTC1880.1176360166442449No Hit
GCGTCCGGGGATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCCA1860.11638456965866782No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGAGATCGGAAGAGCTCGTAT1820.1138816756875137No Hit
GCGTCCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1810.11325595219472515Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGACTTGAGGCTGTACTTTCTTTTATAGAAATGTGTGTAATTT1790.11200450520914808No Hit
GCGTCCGGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGG1780.11137878171635955No Hit
GCGTCCGGGGAGTCGGAGGATGGGTCTGGCAAAGGCGCAGCTTTGGTTAC1770.110753058223571No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCCTCGTATGCCG1770.110753058223571No Hit
GCGTCCGGGGAGGAGCCATACTCAGGTTATGCTGGAGCCTTGAGGTGTCT1750.10950161123799391No Hit
GCGTCCGAGAGTGCAGTGCTGCGTCCGGGAGGAATGAATTGGAAAGCTAT1730.10825016425241686No Hit
GCGTCCGGGTGCTGCGTCCGGGGGGACACTAATTCCCAGCATCCCCACAA1720.10762444075962832No Hit
GCGTCCGGGAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCA1700.10637299377405124No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGATCGGAAGAGCTCGTAT1690.10574727028126271No Hit
GCGTCCGGGGAGGGAAAACTGGTGTGCTCGCGGCGGGCATCAGTCGGCTT1690.10574727028126271No Hit
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGTAAAAAAAAAAAAAAAAAAA1670.10449582329568564No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGCGGAAGGGCTCGTATGCCG1670.10449582329568564No Hit
GCGTCCGGGAACAAAATTTTATTCCAAAAGACAAATAAGAGCTGAAACCC1670.10449582329568564No Hit
GCGTCCGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTG1640.10261865281732002No Hit
GCGTCCGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1640.10261865281732002No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCGGAAGAGCTCG1640.10261865281732002No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGTCCGGGA1630.1019929293245315No Hit
GCGTCCGGGATCTTTAGCAAAAATAATTAAAATGCCCAGTCACTGCCCTT1620.10136720583174297No Hit
GCGTCCGGGATCATCCCTCGTCACCTGCAGCTGGCCATCCGCAACGACGA1610.10074148233895441No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCGATCGGAAGAG1600.10011575884616589No Hit
GCGTCCGGGAGAGAAGCTGCATGAGAACAACCCACGTGGGCATGGCCACA1600.10011575884616589No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTTA302.5140052E-644.00000423
CATGGTG302.5140052E-644.00000429
AAGTAAA302.5140052E-644.00000410
GGTAATT302.5140052E-644.00000428
ATAGGGT302.5140052E-644.00000424
TTTACAT302.5140052E-644.00000427
TAGACTC600.044.00000444
CCTAGAC302.5140052E-644.00000442
TTAGACT302.5140052E-644.00000443
TTATGCT302.5140052E-644.00000427
AGCTTAG302.5140052E-644.00000440
GGGTACC302.5140052E-644.00000414
CGCGCTG302.5140052E-644.00000419
TATGCTG302.5140052E-644.00000428
GGATACT302.5140052E-644.0000048
GCCATAC302.5140052E-644.00000415
TCAGGTT302.5140052E-644.00000422
TTTCATG302.5140052E-644.00000426
TTATATT302.5140052E-644.00000444
GGCCAAG302.5140052E-644.00000434
Produced by FastQC (version 0.11.5)