FastQCFastQC Report
Fri 17 Jun 2016
SRR1527716_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527716_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240547
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGAAG48852.030788161980819No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC15060.6260730751162975Illumina Single End Adapter 2 (100% over 25bp)
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAA12380.5146603366493866No Hit
GCGTCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12040.5005258847543308No Hit
GCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10510.4369208512265794No Hit
GCGTCCGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAA9750.4053261940493958No Hit
GCGTCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9110.37872016695282No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGGAG8420.35003554398932435No Hit
GCGTCCGGGAAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTCTT7540.31345225673153276Illumina Single End Adapter 2 (100% over 27bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGGCCGGGAAG7450.3097107841710767No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA6840.28435191459465303No Hit
GCGTCCTTATGAATGTTTTGACCATTTTCATGGTGGAAGAATTTTATATTTA6630.2756218119535891No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGT6000.24943150403039738Illumina Single End Adapter 2 (95% over 24bp)
GCGTCCGGGGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATCTCTA5770.2398699630425655No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCG5700.23695992882887754Illumina Single End Adapter 2 (100% over 23bp)
GCGTCCGGGACCTATGCTCCTCCTCTCCTTGCGTGGGGTACACCCCATCTTA5610.23321845626842153No Hit
GCGTCCGGGACCTTAACTCTTAAATAGGGTAATTTCAATGACCTAGACTCTT5220.21700540850644576No Hit
GCGTCCGGGAGGAAGTTCCTGTGTGACATAGTGCTGGCACTGATGTTGTCCT5220.21700540850644576No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGACCGGGAAG5080.21118534007906978No Hit
GCGTCCGGGAAGCAGTGGTATCAAGATCGGAAGAGCTCGTATGCCGTCTTCT4490.18665790884941402Illumina Single End Adapter 2 (100% over 29bp)
GCGTCCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4340.18042212124865412Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA4060.16878198439390224Illumina Single End Adapter 2 (100% over 34bp)
GCGTCCGGGAGGGTCAAAACCTACAAACCATCAACTAAAGCCTCCAAGCAAA4030.16753482687375024No Hit
GCGTCCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4010.16670338852698224No Hit
GCGTCCGGGGAAAACTTCACTGAGCGAAAGAGGCAGGGCCATGGCCTTAGGG3990.16587195018021428No Hit
GCGTCCGGGAGTGGCTGATACTGGGGTTTACATGAACACAGAGTGTATTTTC3980.16545623100683027No Hit
GCGTCCGGGAGCACATGTGCCATCATGATGGATTTCTTTAAGTATGTGTAAG3880.16129903927299033No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGGGCAGTGCCGCGTGCCGGCTT3850.16005188175283833No Hit
GCGTCCGGGATGAGAAGAAAGCTGCCAAGCTGAAGGAGAAGTATGAGAAGGA3770.15672612836576638No Hit
GCGTCCGGGCAGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAA3680.1529846558053104No Hit
GCGTCCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCC3630.15090605993839043No Hit
GCGTCCGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG3610.1500746215916224No Hit
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGGAAAAAAAAAAAAAAAAAAAAA3350.13926592308363855No Hit
GCGTCCGGGATGGTGACTGCTGTAAATACTCTGCTGTCAAGAGCATAGTGAA3330.13843448473687053No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTCGT3240.1346930121764146No Hit
GCGTCCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA3200.1330301354828786No Hit
GCGTCCGGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCC3070.12762578622888668No Hit
GCGTCCGGGATGTCTGCCCTTTCCCCACTTGCTCACACAAAAACAAATATAA3070.12762578622888668No Hit
GCGTCCTGTCCCCTGCCTGATACTTGGTGTATGCAAGAGTATTGCCCTCCGT3060.12721006705550267No Hit
GCGTCCGGGATCCCAGACCCCCATAATAACAGGAGGGGCCTAGGGAGCCCTC3050.12679434788211869No Hit
GCGTCCGGGAAGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA3040.12637862870873467Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCT3020.12554719036196668Illumina Single End Adapter 2 (96% over 26bp)
GCGTCCGGGAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGA3020.12554719036196668No Hit
GCGTCCGGGAGTGCAGTGCTGCGTCCGGGGAAAAAAAAAAAAAAAAAAAAAA2920.12138999862812672No Hit
GCGTCCGGGAAGGCAGGAGCGTCTCAGAGTTGAAGGCCAGAATGGACTACAG2910.12097427945474273No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGATCGGAAGAGCTCGTATG2890.12014284110797473No Hit
GCGTCCGGGGATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCCAAC2800.11640136854751879No Hit
GCGTCCGAGAGTGCAGTGCTGCGTCCGGGAGGAATGAATTGGAAAGCTATGC2800.11640136854751879No Hit
GCGTCCGGGGGGACATCTTCACTGACCCTCCTCATCATTGGACATTAGCGAT2780.11556993020075078No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGTCCGGGAAG2760.1147384918539828No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGCGGAAGGGCTCGTATGCCGTC2750.11432277268059879No Hit
GCGTCCGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGAAGCA2750.11432277268059879No Hit
GCGTCCGGGGAGGGAAAACTGGTGTGCTCGCGGCGGGCATCAGTCGGCTTAG2720.11307561516044683No Hit
GCGTCCGGGATCATCCCTCGTCACCTGCAGCTGGCCATCCGCAACGACGAGG2700.11224417681367882No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGGGCAGTGCTCGGATGCCGTCT2680.11141273846691083No Hit
GCGTCCGGGAGAGAAGCTGCATGAGAACAACCCACGTGGGCATGGCCACAGT2650.11016558094675885No Hit
GCGTCCGGGGGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTCGG2620.10891842342660686No Hit
GCGTCCGGGATTGCACCTCTGGACATTGGTGGTGCTGACCAAGAATTCCGTG2610.10850270425322288No Hit
GCGTCCGGGGAGAATGTCAACAAAACAGCCACATTTTTTTTTATTTTTTATT2590.10767126590645487No Hit
GCGTCCGGGGAGTCGGAGGATGGGTCTGGCAAAGGCGCAGCTTTGGTTACTG2550.1060083892129189No Hit
GCGTCCGGGGAGAGGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTA2510.1043455125193829No Hit
GCGTCCGGGAAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA2510.1043455125193829Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGAGATCGGAAGAGCTCGTA2500.1039297933459989No Hit
GCGTCCGGGGGCAGTGACTTATCTGTCTATTTCTTACCCAAAAAGCCCATCA2490.10351407417261492No Hit
GCGTCCGGGATCTTTAGCAAAAATAATTAAAATGCCCAGTCACTGCCCTTTC2470.10268263582584693No Hit
GCGTCCGGGACCATGGGCAGGACTCGCTGAAGGAGTTTGTGCAGTTTGTGTG2470.10268263582584693No Hit
GCGTCCGGGTGCTGCGTCCGGGGGGACACTAATTCCCAGCATCCCCACAAAG2460.10226691665246293No Hit
GCGTCCGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA2440.10143547830569494Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGGCCGGTTCTGGTGGCGCAGGCCGGTAGGATTTGCTGAAGGAGG2440.10143547830569494No Hit
GCGTCCGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGGAA2410.10018832078554296No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAATGA351.01506885E-746.00000431
CCTTATG700.046.0000045
GATGTTG700.046.00000442
CCCTCCG351.01506885E-746.00000445
TTCAGGA351.01506885E-746.00000426
TTTACCT351.01506885E-746.00000426
CGACGCT351.01506885E-746.00000439
GTCCTTA700.046.0000043
GAGTATT351.01506885E-746.00000437
CAGTTGG351.01506885E-746.00000427
ATCAGAC351.01506885E-746.00000434
AACCAAC351.01506885E-746.00000440
CGGCACC351.01506885E-746.00000427
ACCTGTC351.01506885E-746.00000429
ATTAAGG351.01506885E-746.00000410
TATTGCC351.01506885E-746.00000440
AGAGTAT351.01506885E-746.00000436
TAGCAAC351.01506885E-746.00000438
CAGATCA351.01506885E-746.00000431
GTAGAGA351.01506885E-746.00000413
Produced by FastQC (version 0.11.5)