FastQCFastQC Report
Fri 17 Jun 2016
SRR1527715_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527715_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242144
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGAAG39601.6353905114312144No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGGAG15600.6442447469274482No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC13890.5736256112065549Illumina Single End Adapter 2 (100% over 25bp)
GCGTCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11940.49309501784062376No Hit
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAA11740.4848354698030924No Hit
GCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9860.4071957182502974No Hit
GCGTCCGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAA9700.4005880798202722No Hit
GCGTCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8330.3440101757631822No Hit
GCGTCCGGGAAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTCTT7780.32129641865997094Illumina Single End Adapter 2 (100% over 27bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA7170.2961047971455002No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGGCCGGGAAG6280.25934980837848554No Hit
GCGTCCTTATGAATGTTTTGACCATTTTCATGGTGGAAGAATTTTATATTTA6170.2548070569578432No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGT5840.24117880269591646Illumina Single End Adapter 2 (95% over 24bp)
GCGTCCGGGGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATCTCTA5530.2283765032377428No Hit
GCGTCCGGGACCTATGCTCCTCCTCTCCTTGCGTGGGGTACACCCCATCTTA5180.2139222941720629No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCG5110.2110314523589269Illumina Single End Adapter 2 (100% over 23bp)
GCGTCCGGGACCTTAACTCTTAAATAGGGTAATTTCAATGACCTAGACTCTT4880.20153297211576582No Hit
GCGTCCGGGAGGAAGTTCCTGTGTGACATAGTGCTGGCACTGATGTTGTCCT4590.1895566274613453No Hit
GCGTCCGGGAGGGTCAAAACCTACAAACCATCAACTAAAGCCTCCAAGCAAA4470.18460089863882648No Hit
GCGTCCGGGAAGCAGTGGTATCAAGATCGGAAGAGCTCGTATGCCGTCTTCT4440.18336196643319677Illumina Single End Adapter 2 (100% over 29bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGACCGGGAAG4280.17675432800317167No Hit
GCGTCCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA4100.1693207347693934Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA3990.16477798334875116Illumina Single End Adapter 2 (100% over 34bp)
GCGTCCGGGAGCACATGTGCCATCATGATGGATTTCTTTAAGTATGTGTAAG3960.16353905114312145No Hit
GCGTCCGGGATGAGAAGAAAGCTGCCAAGCTGAAGGAGAAGTATGAGAAGGA3700.15280163869433064No Hit
GCGTCCGGGAGTGGCTGATACTGGGGTTTACATGAACACAGAGTGTATTTTC3660.15114972908682436No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGGGCAGTGCCGCGTGCCGGCTT3560.14701995506805868No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGGCCGGGGAG3540.14619400026430554No Hit
GCGTCCGGGCAGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAA3510.14495506805867583No Hit
GCGTCCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3510.14495506805867583No Hit
GCGTCCGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG3480.1437161358530461No Hit
GCGTCCGGGATGGTGACTGCTGTAAATACTCTGCTGTCAAGAGCATAGTGAA3390.13999933923615698No Hit
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGGAAAAAAAAAAAAAAAAAAAAA3370.13917338443240387No Hit
GCGTCCGGGGAAAACTTCACTGAGCGAAAGAGGCAGGGCCATGGCCTTAGGG3350.13834742962865074No Hit
GCGTCCGGGATGTCTGCCCTTTCCCCACTTGCTCACACAAAAACAAATATAA3170.13091383639487247No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTCGT3080.12719703977798336No Hit
GCGTCCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA3080.12719703977798336No Hit
GCGTCCTGTCCCCTGCCTGATACTTGGTGTATGCAAGAGTATTGCCCTCCGT3070.1267840623761068No Hit
GCGTCCGGGAAGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA3040.12554513017047705Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGA2920.12058940134795823No Hit
GCGTCCGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA2860.11811153693669883Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCC2840.1172855821329457No Hit
GCGTCCGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGAAGCA2790.11522069512356285No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGCGGAAGGGCTCGTATGCCGTC2770.1143947403198097No Hit
GCGTCCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCC2770.1143947403198097No Hit
GCGTCCGGGATCCCAGACCCCCATAATAACAGGAGGGGCCTAGGGAGCCCTC2750.11356878551605656No Hit
GCGTCCGAGAGTGCAGTGCTGCGTCCGGGAGGAATGAATTGGAAAGCTATGC2750.11356878551605656No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGATCGGAAGAGCTCGTATG2740.11315580811417998No Hit
GCGTCCGGGGGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTCGG2710.11191687590855028No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCT2630.10861305669353774Illumina Single End Adapter 2 (96% over 26bp)
GCGTCCGGGATCATCCCTCGTCACCTGCAGCTGGCCATCCGCAACGACGAGG2620.10820007929166116No Hit
GCGTCCGGGATCTTTAGCAAAAATAATTAAAATGCCCAGTCACTGCCCTTTC2620.10820007929166116No Hit
GCGTCCGGGGAGAGGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTA2600.10737412448790801No Hit
GCGTCCGGGAGTGCAGTGCTGCGTCCGGGGAAAAAAAAAAAAAAAAAAAAAA2590.10696114708603145No Hit
GCGTCCGGGGGGACATCTTCACTGACCCTCCTCATCATTGGACATTAGCGAT2560.10572221488040175No Hit
GCGTCCGGGGAGTCGGAGGATGGGTCTGGCAAAGGCGCAGCTTTGGTTACTG2560.10572221488040175No Hit
GCGTCCGGGATTGCACCTCTGGACATTGGTGGTGCTGACCAAGAATTCCGTG2550.10530923747852516No Hit
GCGTCCGGGAAGGCAGGAGCGTCTCAGAGTTGAAGGCCAGAATGGACTACAG2470.10200541826351263No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGAGATCGGAAGAGCTCGTA2450.10117946345975948No Hit
GCGTCCGGGACATGGGCTGGGGGCTGGGGCTGGATGGACAGACACCTCCCCC2430.10035350865600634No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCT750.046.00000412
ACCTATG750.046.00000410
CTTGCGT750.046.00000428
TTGCGTG750.046.00000429
CTTGTTC253.4091136E-546.025
CTTGTTA253.4091136E-546.029
ATTTAGT206.301538E-446.016
TATTTGA253.4091136E-546.042
TCGCCAA206.301538E-446.034
CTGCTCC253.4091136E-546.042
CTTCGGG206.301538E-446.043
TTAGGTC206.301538E-446.017
AGACTTA253.4091136E-546.020
GACAGTC253.4091136E-546.045
AGCGTGA253.4091136E-546.026
CTAGAAG301.8552764E-646.013
AGACTGG206.301538E-446.024
CGTGGCT253.4091136E-546.040
AAGTAAT253.4091136E-546.034
AAGTAAA301.8552764E-646.010
Produced by FastQC (version 0.11.5)