FastQCFastQC Report
Fri 17 Jun 2016
SRR1527714_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527714_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2449086
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC144210.5888319152532823No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC91130.37209799900860974No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT87210.35609202780139204No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG84900.3466599376257102No Hit
GCCTAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT69110.2821869056456164No Hit
GCCTAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT62930.25695300205872723No Hit
GCCTAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC48050.19619564196602324No Hit
GCCTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT45870.1872943620599685No Hit
GCCTAGGGAGCCCTCCCTACTCTCTTGAATACCATCAATAAAGTTCGCTG45610.18623274152071426No Hit
GCCTAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA42340.17288082166163213No Hit
GCCTAGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCCTAGGGGA38230.1560990508295748No Hit
GCCTAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG32180.13139595751231276No Hit
GCCTAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA31720.12951770578901678No Hit
GCCTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC27860.11375672393701161No Hit
GCCTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC27270.11134766194408853No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCGG207.858227E-444.00000413
AGGGGAT305050.041.201775
CCTAGGG2579050.041.0272032
GCCTAGG2605900.041.0173071
TAGGGGT136100.040.993394
CTAGGGG2469550.040.95243
TAGGGGG1264050.040.863734
TAGGGGA901800.040.852964
AGGGGGG454100.040.5892945
CTAGGGT35450.040.5246853
AGGGGGA339600.040.171385
TAGGGGC175750.040.169564
AGGGGGC269750.040.028175
AGGGGAG277000.039.9256325
AGGGGTA39750.039.9044045
AGGGGGT220200.039.883745
AGGGGAC153150.039.6761325
CATCGTA501.3496901E-939.638
CTAGGGA59650.039.463543
CGGGATA452.3528628E-839.1111119
Produced by FastQC (version 0.11.5)