##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527714_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2449086 Sequences flagged as poor quality 0 Sequence length 50 %GC 56 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.541611441982845 34.0 31.0 34.0 31.0 34.0 2 32.83363793676498 34.0 31.0 34.0 31.0 34.0 3 33.18195481906311 34.0 33.0 34.0 31.0 34.0 4 36.490160819179074 37.0 37.0 37.0 35.0 37.0 5 36.24563326890113 37.0 37.0 37.0 35.0 37.0 6 36.411252606074264 37.0 37.0 37.0 35.0 37.0 7 36.656553097767905 37.0 37.0 37.0 35.0 37.0 8 36.70247471914012 37.0 37.0 37.0 35.0 37.0 9 38.68134071241271 39.0 39.0 39.0 39.0 39.0 10 38.15735911274655 39.0 39.0 39.0 37.0 39.0 11 37.265616642290226 39.0 35.0 39.0 35.0 39.0 12 37.0826598167643 39.0 37.0 39.0 34.0 39.0 13 37.00208200120372 39.0 35.0 39.0 33.0 39.0 14 37.74315356831079 40.0 36.0 40.0 33.0 40.0 15 37.837482228063855 40.0 36.0 40.0 34.0 40.0 16 37.85699113873502 40.0 36.0 40.0 34.0 40.0 17 37.793976610049626 40.0 36.0 40.0 34.0 40.0 18 37.76830295057013 40.0 36.0 40.0 34.0 40.0 19 37.66620527004768 40.0 36.0 40.0 34.0 40.0 20 37.60480113805722 40.0 36.0 40.0 33.0 40.0 21 37.57909767153951 40.0 36.0 40.0 33.0 40.0 22 37.5915431307843 40.0 35.0 40.0 33.0 40.0 23 37.50267201723418 40.0 35.0 40.0 33.0 40.0 24 37.44425838863968 40.0 35.0 40.0 33.0 40.0 25 37.40307649465964 40.0 35.0 40.0 33.0 40.0 26 37.2847874676512 40.0 35.0 40.0 33.0 40.0 27 37.21439794274272 39.0 35.0 40.0 33.0 40.0 28 37.0659160192823 39.0 35.0 40.0 33.0 40.0 29 37.17866134549787 40.0 35.0 40.0 33.0 40.0 30 37.283286907850524 40.0 35.0 40.0 33.0 40.0 31 37.32662225826288 40.0 35.0 40.0 33.0 40.0 32 37.274121039440836 40.0 35.0 40.0 33.0 40.0 33 37.214822182642834 40.0 35.0 40.0 33.0 40.0 34 36.873658989516905 40.0 35.0 40.0 33.0 40.0 35 36.87867759645843 40.0 35.0 40.0 33.0 40.0 36 36.85624351288603 40.0 35.0 40.0 33.0 40.0 37 36.76318063146823 39.0 35.0 40.0 33.0 40.0 38 36.70078960069185 39.0 35.0 40.0 32.0 40.0 39 36.577969495558754 39.0 35.0 40.0 32.0 40.0 40 36.41348527573144 39.0 35.0 40.0 32.0 40.0 41 36.273510199315176 39.0 35.0 40.0 31.0 40.0 42 36.18187233931352 39.0 35.0 40.0 31.0 40.0 43 36.08400399169323 39.0 35.0 40.0 31.0 40.0 44 35.80540699673266 38.0 35.0 40.0 30.0 40.0 45 35.81750171288391 38.0 35.0 40.0 30.0 40.0 46 35.67041418717024 38.0 35.0 40.0 30.0 40.0 47 35.59534495726161 37.0 35.0 40.0 30.0 40.0 48 35.509380234095495 37.0 35.0 40.0 30.0 40.0 49 35.428697073112176 37.0 35.0 40.0 30.0 40.0 50 34.92237226459177 36.0 34.0 40.0 28.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 0.0 13 2.0 14 14.0 15 31.0 16 83.0 17 205.0 18 423.0 19 763.0 20 1335.0 21 2196.0 22 3487.0 23 5313.0 24 7377.0 25 10300.0 26 14102.0 27 18547.0 28 24217.0 29 31193.0 30 39433.0 31 48115.0 32 59154.0 33 85695.0 34 206056.0 35 214744.0 36 167262.0 37 292329.0 38 707527.0 39 509182.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 100.0 0.0 5 0.0 100.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 99.19427084226524 0.6772322409257984 0.10350800257728802 0.024988914231676634 8 98.84422188522576 0.9252022999600666 0.12437292932955396 0.1062028854846257 9 95.2708479816552 2.4191882196051915 1.3078348412428147 1.0021289574967969 10 50.50279982001449 36.44963876319574 5.899588662872598 7.147972753917175 11 33.105942380136916 24.502610361579787 23.764253276528468 18.627193981754825 12 31.750579603982874 16.639391185119674 27.379969506991586 24.230059703905866 13 26.899872033893462 16.66164438488481 27.800942882365092 28.637540698856633 14 23.88944283704206 19.942745987686834 28.512677790816653 27.655133384454444 15 26.06613242654607 20.078592585152176 27.09721095951714 26.758064028784617 16 29.65355238648214 21.28508349645541 26.868880880459077 22.19248323660337 17 29.68940249546157 23.444705494212943 23.253736291824787 23.6121557185007 18 30.728647340273064 21.13531333730216 23.87784667422867 24.258192648196104 19 29.831659647721644 23.193428078883304 23.0602763643253 23.91463590906975 20 30.993154180784177 23.465692915642816 23.252960492199946 22.28819241137306 21 30.566954365832803 21.449512185362213 25.465418527564974 22.518114921240006 22 28.48344239442796 20.89828613613405 25.215856037721828 25.402415431716157 23 26.20410226508991 23.111846623597536 26.009989032643198 24.674062078669348 24 26.8987287502358 24.13190063558405 24.527925928285082 24.441444685895064 25 27.135919277640717 24.455694900056592 22.89560268606329 25.5127831362394 26 25.786722066926192 25.090911466563444 23.390685341388583 25.73168112512178 27 25.13799025432345 22.564867056526396 24.370193615087423 27.926949074062733 28 26.407075945883484 24.510776673420207 23.56307618433979 25.519071196356517 29 29.25850705120196 23.23327968066454 22.833171232043302 24.675042036090197 30 26.916449646929507 22.932391920904372 24.773976904036854 25.377181528129267 31 28.745621836064554 23.448502829218736 23.385009754659496 24.420865580057214 32 29.89882756260907 22.785234981540054 24.294859388359576 23.021078067491302 33 31.06893755466325 22.477732509189142 23.123279460174125 23.330050475973486 34 27.396179635994816 23.716031205110806 26.014358009477824 22.873431149416557 35 26.02860822363935 26.258979880657517 26.10492240778805 21.607489487915082 36 26.81494239075312 25.854706612997663 24.347287110375053 22.98306388587416 37 27.191409366596353 26.476693754323044 23.297711881085434 23.034184997995172 38 28.372993026786318 26.16894629261692 22.84080673361409 22.61725394698267 39 28.124002178772002 24.37231685616593 22.488961187969718 25.014719777092353 40 27.173974290817064 24.573943095505836 23.267578190394296 24.984504423282807 41 25.70897877820542 25.065391742062143 24.015244870943693 25.21038460878875 42 24.634292140006515 23.815251893971872 23.91892322278597 27.63153274323564 43 24.569288297756795 23.455566688960698 24.736248543334124 27.238896469948383 44 25.365013723487046 22.973550132580073 24.867930321760852 26.793505822172026 45 26.19773254185439 24.030393379407663 24.0917223813292 25.68015169740875 46 25.97417975522297 25.277470860557777 23.445481293837783 25.302868090381477 47 25.319486534976722 24.693212079935126 24.50363115055984 25.48367023452831 48 26.406626798732262 24.206132410213442 24.976787258593614 24.410453532460682 49 27.418392004200754 23.92651789279756 24.38521962887379 24.2698704741279 50 25.01990538511102 24.36088401958935 24.747028074963477 25.872182520336157 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 6.5 8 13.0 9 18.5 10 24.0 11 179.5 12 335.0 13 1829.0 14 3323.0 15 4872.0 16 6421.0 17 8138.0 18 9855.0 19 8281.5 20 6708.0 21 6172.0 22 5636.0 23 6369.5 24 7103.0 25 7391.5 26 7680.0 27 8680.0 28 9680.0 29 11889.5 30 14099.0 31 16568.5 32 19038.0 33 21422.0 34 23806.0 35 27473.5 36 31141.0 37 40058.0 38 48975.0 39 49618.0 40 50261.0 41 56743.5 42 63226.0 43 67980.5 44 72735.0 45 75489.5 46 78244.0 47 85913.5 48 93583.0 49 101447.0 50 109311.0 51 117550.5 52 125790.0 53 129635.5 54 133481.0 55 141826.5 56 150172.0 57 158217.0 58 166262.0 59 179714.0 60 193166.0 61 198458.5 62 203751.0 63 206418.5 64 209086.0 65 202988.0 66 196890.0 67 166314.0 68 135738.0 69 113841.0 70 91944.0 71 77938.5 72 63933.0 73 55170.5 74 46408.0 75 37382.5 76 28357.0 77 26667.0 78 24977.0 79 18169.0 80 11361.0 81 7438.5 82 3516.0 83 2962.0 84 2408.0 85 1459.5 86 511.0 87 301.5 88 92.0 89 63.0 90 34.0 91 22.0 92 10.0 93 5.5 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2449086.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 8.88691932720361 #Duplication Level Percentage of deduplicated Percentage of total 1 64.75845585299727 5.755031729198632 2 9.635840643831166 1.7126587690303456 3 3.8242107466173727 1.0195635718624103 4 1.8155749982853016 0.6453947416899724 5 0.9823946931391917 0.43652311927004706 6 0.7001969165280976 0.3733556106205154 7 0.540293792182189 0.3361083140878418 8 0.4357174189570685 0.3097748441383075 9 0.3397129166987168 0.27171011565994824 >10 10.679180569634589 27.269252601329008 >50 4.389334054765093 26.719457009077495 >100 1.8351151875363176 27.180286747824212 >500 0.04155858821647132 2.4312559962831646 >1k 0.019611918034739273 3.3008777730652703 >5k 0.0023347521469927707 1.6403134945907172 >10k+ 4.669504293985541E-4 0.5984355622721294 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 14421 0.5888319152532823 No Hit GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC 9113 0.37209799900860974 No Hit GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT 8721 0.35609202780139204 No Hit GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG 8490 0.3466599376257102 No Hit GCCTAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT 6911 0.2821869056456164 No Hit GCCTAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT 6293 0.25695300205872723 No Hit GCCTAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC 4805 0.19619564196602324 No Hit GCCTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT 4587 0.1872943620599685 No Hit GCCTAGGGAGCCCTCCCTACTCTCTTGAATACCATCAATAAAGTTCGCTG 4561 0.18623274152071426 No Hit GCCTAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA 4234 0.17288082166163213 No Hit GCCTAGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCCTAGGGGA 3823 0.1560990508295748 No Hit GCCTAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG 3218 0.13139595751231276 No Hit GCCTAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA 3172 0.12951770578901678 No Hit GCCTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC 2786 0.11375672393701161 No Hit GCCTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC 2727 0.11134766194408853 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.04969200754893867 0.0 0.0 0.0 0.0 6 0.04969200754893867 0.0 0.0 0.0 0.0 7 0.050957785884203334 0.0 0.0 0.0 0.0 8 0.06128817036233109 0.0 0.0 0.0 0.0 9 0.0834188754498617 0.0 0.0 0.0 0.0 10 0.11861567948205984 0.0 0.0 0.0 0.0 11 0.14037890053677168 0.0 0.0 0.0 0.0 12 0.15646653486239356 0.0 0.0 0.0 0.0 13 0.16769521364296722 0.0 0.0 0.0 0.0 14 0.20248370208314448 0.0 0.0 0.0 0.0 15 0.21971462006642478 0.0 0.0 0.0 0.0 16 0.23837464262177807 0.0 0.0 0.0 0.0 17 0.2609953264197337 0.0 0.0 0.0 0.0 18 0.27577635085088886 0.0 0.0 0.0 0.0 19 0.2963554566887402 0.0 0.0 0.0 0.0 20 0.3132597221984038 0.0 0.0 0.0 0.0 21 0.32979650367524865 0.0 0.0 0.0 0.0 22 0.34400588627757456 0.0 0.0 0.0 0.0 23 0.3626659088329279 0.0 0.0 0.0 0.0 24 0.3830408568747688 0.0 0.0 0.0 0.0 25 0.3990468280819865 0.0 0.0 0.0 0.0 26 0.42983382372035933 0.0 0.0 0.0 0.0 27 0.4489429934269356 0.0 0.0 0.0 0.0 28 0.4910811625234884 0.0 0.0 0.0 0.0 29 0.5073729546451207 0.0 0.0 4.0831559202086E-5 0.0 30 0.5323618688767973 0.0 0.0 4.0831559202086E-5 0.0 31 0.5471837248671545 0.0 0.0 4.0831559202086E-5 0.0 32 0.5701718926979289 0.0 0.0 4.0831559202086E-5 0.0 33 0.5883011049836552 0.0 0.0 4.0831559202086E-5 0.0 34 0.6045520655460853 0.0 0.0 4.0831559202086E-5 0.0 35 0.6433828783472691 0.0 0.0 4.0831559202086E-5 0.0 36 0.660858785685762 0.0 0.0 4.0831559202086E-5 0.0 37 0.680376270984359 0.0 0.0 4.0831559202086E-5 0.0 38 0.6964639053099809 0.0 0.0 4.0831559202086E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTATCGG 20 7.858227E-4 44.000004 13 AGGGGAT 30505 0.0 41.20177 5 CCTAGGG 257905 0.0 41.027203 2 GCCTAGG 260590 0.0 41.017307 1 TAGGGGT 13610 0.0 40.99339 4 CTAGGGG 246955 0.0 40.9524 3 TAGGGGG 126405 0.0 40.86373 4 TAGGGGA 90180 0.0 40.85296 4 AGGGGGG 45410 0.0 40.589294 5 CTAGGGT 3545 0.0 40.524685 3 AGGGGGA 33960 0.0 40.17138 5 TAGGGGC 17575 0.0 40.16956 4 AGGGGGC 26975 0.0 40.02817 5 AGGGGAG 27700 0.0 39.925632 5 AGGGGTA 3975 0.0 39.904404 5 AGGGGGT 22020 0.0 39.88374 5 AGGGGAC 15315 0.0 39.676132 5 CATCGTA 50 1.3496901E-9 39.6 38 CTAGGGA 5965 0.0 39.46354 3 CGGGATA 45 2.3528628E-8 39.11111 19 >>END_MODULE