FastQCFastQC Report
Fri 17 Jun 2016
SRR1527713_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527713_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3673742
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC208430.5673506740538666No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA135270.3682076748993261No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC133030.36211034961083277No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT108170.2944409269894293No Hit
GCCTAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG102300.2784626683093151No Hit
GCCTAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT96710.26324657529026263No Hit
GCCTAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT72580.19756422742805563No Hit
GCCTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC69570.18937094657164277No Hit
GCCTAGGGAGCCCTCCCTACTCTCTTGAATACCATCAATAAAGTTCGCTGCA60850.16563493027000808No Hit
GCCTAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG60720.165281067641658No Hit
GCCTAGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCCTAGGGGAAG54370.1479962392568667No Hit
GCCTAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC47350.12888765732596355No Hit
GCCTAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT44770.12186484516332394No Hit
GCCTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC43410.11816289766673871No Hit
GCCTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC41220.11220167338914927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATA301.8625287E-646.019
GCCTAGG3911200.042.8780171
AGGGGAT450250.042.8481945
CCTAGGG3872750.042.8297962
TAGGGGA1365200.042.7804684
CTAGGGG3698600.042.7563973
TAGGGGT207250.042.7150764
TAGGGGG1871800.042.640564
CTAGGGT49500.042.1434333
AGGGGGG676200.042.125855
TAGGGGC263350.042.02624
CTAGGGA97550.041.99183
TAGGGTA18100.041.93374
AGGGGAG427350.041.8935285
AGGGGGA502900.041.8747255
AGGGGAC232450.041.78495
AGGGGGC401300.041.460755
AGGGGGT328800.041.4601555
TCGTAAC508.731149E-1041.39999817
AGGGGTA60050.041.1740235
Produced by FastQC (version 0.11.5)