FastQCFastQC Report
Fri 17 Jun 2016
SRR1527712_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527712_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3710199
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC195590.527168488806126No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA129120.34801367797252925No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC128280.3457496484689905No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT106150.2861032521436182No Hit
GCCTAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG100040.2696351327785922No Hit
GCCTAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT93010.2506873620525476No Hit
GCCTAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT68590.18486879005681367No Hit
GCCTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC66280.1786427089220821No Hit
GCCTAGGGAGCCCTCCCTACTCTCTTGAATACCATCAATAAAGTTCGCTGCA59030.1591019780879678No Hit
GCCTAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG56780.1530376133463461No Hit
GCCTAGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCCTAGGGGAAG50520.13616520299854537No Hit
GCCTAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC45140.1216646330830233No Hit
GCCTAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT43000.11589674839543648No Hit
GCCTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC41870.11285108965853315No Hit
GCCTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC40040.10791873966868085No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACATA206.313281E-446.00000443
TTATCGG253.418638E-546.013
TAGGGGT206800.043.0971954
AGGGGAT456500.042.861125
GCCTAGG3957150.042.802671
CCTAGGG3915950.042.7678572
CTAGGGG3744350.042.7038883
TAGGGGA1377950.042.6817364
TAGGGGG1902950.042.5758934
CTAGGGT51450.042.1107863
AGGGGGG677350.042.040755
TAACACG554.7293724E-1141.8181846
AGGGGGA512200.041.7924655
TAGGGGC267450.041.700134
AGGGGGT327300.041.5798955
TAGGGTA20700.041.5555574
AGGGGGC422800.041.457665
CTAGGGA92650.041.4074443
AGGGGAG429800.041.397865
AGGGGAC235950.041.340545
Produced by FastQC (version 0.11.5)