FastQCFastQC Report
Fri 17 Jun 2016
SRR1527711_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527711_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568086
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC128890.8219574691694206No Hit
TTCATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG97940.6245830904682524No Hit
TTCATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT88130.56202274620142No Hit
TTCATAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC53330.34009614268605165No Hit
TTCATAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG47290.30157784713338426No Hit
TTCATAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT42880.27345438961893676No Hit
TTCATAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG36380.23200258149106617No Hit
TTCATAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC36170.23066336922847344No Hit
TTCATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC34810.22199037552787285No Hit
TTCATAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT30940.1973106066886638No Hit
TTCATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG22030.1404897435472289No Hit
TTCATAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG21480.13698228285948602No Hit
TTCATAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC20010.12760779702133684No Hit
TTCATAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT19700.12563086463369993No Hit
TTCATAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA19010.12123059577089522No Hit
TTCATAGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTG18760.11963629545828482No Hit
TTCATAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG18730.11944497942077156No Hit
TTCATAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT18520.11810576715817883No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT18410.11740427502063024No Hit
TTCATAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA17290.11026180962013563No Hit
TTCATAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG17200.10968786150759588No Hit
TTCATAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG17190.10962408949509146No Hit
TTCATAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC16970.10822110521999431No Hit
TTCATAGGGGAGTGCAGTGCTTTCATAGGGAAAAAAAAAAAAAAAAAAAA16930.10796601716997664No Hit
TTCATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC16510.10528759264479116No Hit
TTCATAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAG15920.1015250439070306No Hit
TTCATAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT15760.10050469170695994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACG302.528308E-644.00000411
CCGATTA351.4465331E-744.031
ATTACGC254.4435852E-544.027
AATATCG207.8573613E-444.018
TTACGTC254.4435852E-544.030
CCGAATA207.8573613E-444.029
TATCGTA207.8573613E-444.026
CGTTTAT254.4435852E-544.031
CGATACT351.4465331E-744.026
GCGTATC254.4435852E-544.026
TAGGGAT158900.041.5632485
AGGGATA19900.041.3467336
TAGGGGC186500.040.8504035
ATAGGGG948400.040.7338684
TAGGGAG184800.040.6904755
AGGGATC42500.040.6352926
CATAGGG1653400.040.600343
TCATAGG1679650.040.576192
TTCATAG1694000.040.5662351
TAGGGGT162350.040.4360925
Produced by FastQC (version 0.11.5)