##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527711_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1568086
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5956879916025	34.0	31.0	34.0	31.0	34.0
2	33.02842956317447	34.0	31.0	34.0	31.0	34.0
3	33.13501045223285	34.0	33.0	34.0	31.0	34.0
4	36.390008583712884	37.0	37.0	37.0	35.0	37.0
5	36.45919228919843	37.0	37.0	37.0	35.0	37.0
6	36.42361069482158	37.0	37.0	37.0	35.0	37.0
7	36.55960451148725	37.0	37.0	37.0	35.0	37.0
8	36.696868028921884	37.0	37.0	37.0	35.0	37.0
9	38.766436279642825	39.0	39.0	39.0	39.0	39.0
10	38.298018093395385	39.0	39.0	39.0	37.0	39.0
11	38.090393639124386	39.0	39.0	39.0	37.0	39.0
12	37.69295752911511	39.0	37.0	39.0	35.0	39.0
13	37.57271093549716	39.0	37.0	39.0	35.0	39.0
14	38.357375807194245	40.0	38.0	40.0	35.0	40.0
15	38.40968416273087	40.0	38.0	40.0	35.0	40.0
16	38.44270849940628	40.0	38.0	40.0	35.0	40.0
17	38.367866940971346	40.0	38.0	40.0	35.0	40.0
18	38.27814928517951	40.0	38.0	40.0	34.0	40.0
19	38.30572430338642	40.0	38.0	40.0	34.0	40.0
20	38.209652404268645	40.0	38.0	40.0	34.0	40.0
21	38.21041447981807	40.0	38.0	40.0	34.0	40.0
22	38.261921221157515	40.0	38.0	40.0	34.0	40.0
23	38.21730887208992	40.0	38.0	40.0	35.0	40.0
24	38.15322437672424	40.0	38.0	40.0	35.0	40.0
25	38.09947604914527	40.0	38.0	40.0	35.0	40.0
26	37.98571889551976	40.0	38.0	40.0	34.0	40.0
27	37.85966586016328	40.0	37.0	40.0	34.0	40.0
28	37.72394626315138	40.0	37.0	40.0	34.0	40.0
29	37.730813871177986	40.0	37.0	40.0	34.0	40.0
30	37.8404564545567	40.0	37.0	40.0	34.0	40.0
31	37.82627355897572	40.0	37.0	40.0	34.0	40.0
32	37.815545831032225	40.0	37.0	40.0	34.0	40.0
33	37.68330308414207	40.0	37.0	40.0	34.0	40.0
34	37.344894348906884	40.0	36.0	40.0	33.0	40.0
35	37.29209622431423	40.0	36.0	40.0	33.0	40.0
36	37.27185243666482	40.0	36.0	40.0	33.0	40.0
37	37.18958909141463	40.0	35.0	40.0	33.0	40.0
38	37.08715338316904	40.0	35.0	40.0	33.0	40.0
39	36.95463450346473	40.0	35.0	40.0	33.0	40.0
40	36.73121244625614	40.0	35.0	40.0	32.0	40.0
41	36.604831622755384	40.0	35.0	40.0	32.0	40.0
42	36.51776943356423	39.0	35.0	40.0	32.0	40.0
43	36.37345209382649	39.0	35.0	40.0	31.0	40.0
44	36.09919736545062	39.0	35.0	40.0	31.0	40.0
45	36.04131342286074	39.0	35.0	40.0	30.0	40.0
46	35.908522874383166	39.0	35.0	40.0	30.0	40.0
47	35.796771988271054	38.0	35.0	40.0	30.0	40.0
48	35.70110376599243	38.0	35.0	40.0	30.0	40.0
49	35.58209243625669	37.0	35.0	40.0	30.0	40.0
50	35.04452243052996	37.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	3.0
14	5.0
15	14.0
16	37.0
17	99.0
18	202.0
19	405.0
20	647.0
21	1031.0
22	1728.0
23	2647.0
24	4009.0
25	5688.0
26	7671.0
27	10029.0
28	13334.0
29	16634.0
30	19992.0
31	24527.0
32	30477.0
33	39954.0
34	83320.0
35	111491.0
36	113313.0
37	216172.0
38	488379.0
39	376274.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.64651173468802	0.27402833773147645	0.05446129867877145	0.024998628901731153
8	99.12319860007678	0.7828652255042133	0.06817228136722094	0.025763893051784153
9	98.39045817640104	0.8648760335848927	0.3151612857968249	0.42950450421724323
10	57.42433769576414	31.659296747754905	4.059981404081154	6.856384152399804
11	34.68725567347709	25.240898777235433	21.963081106520942	18.10876444276653
12	32.12260041859949	18.056216304462893	26.616907491043225	23.204275785894396
13	24.21327656773927	18.15869792855749	29.9754605295883	27.65256497411494
14	25.822180671213186	19.63699695042236	29.649904405753258	24.890917972611195
15	26.089768035681715	18.901705646246445	28.447164249919965	26.561362068151873
16	29.901484995083177	21.028757351318742	25.42743191381085	23.642325739787232
17	31.028527772073726	20.760787354775186	23.092929852061687	25.117755021089405
18	28.29066773123413	21.848737888100526	25.041292378096607	24.819302002568737
19	29.139026813580376	21.492316110213345	23.539461483617607	25.829195592588672
20	27.954142821248322	22.576376550775915	24.34483822953588	25.124642398439885
21	29.43709720002602	21.31496614343856	26.187084126763455	23.060852529771964
22	27.682091415904488	20.5819706317128	25.965412611298106	25.77052534108461
23	28.637204847183128	21.80537291959752	25.924726067320286	23.632696165899063
24	29.546083569396064	21.53281133815365	23.68849667684043	25.23260841560986
25	27.43771706398756	23.115887776563277	22.61310922997846	26.83328592947071
26	27.128295259316133	21.741537135080602	24.266207338117933	26.863960267485332
27	26.899162418387768	21.899946814141572	23.32493243355275	27.87595833391791
28	29.649139141603204	22.584348052338964	21.49072180990073	26.2757909961571
29	30.987267279983367	22.531736142022822	21.560233303530545	24.920763274463262
30	29.98292185505132	21.608062312908856	23.142416933765112	25.26659889827471
31	30.965138391644338	22.5935312221396	22.33978238438453	24.10154800183153
32	32.50274538513832	23.02297195434434	21.501052875926447	22.973229784590895
33	32.20435613863015	22.350368538460263	21.94337555465708	23.501899768252507
34	29.259683461238733	23.925792335369362	24.777722650415857	22.036801552976048
35	27.76633424442282	26.541273884212984	24.79003064882921	20.90236122253499
36	28.701614579812585	26.310291654921986	22.225949342064148	22.762144423201278
37	29.45744047201493	26.645477352645198	21.0671481028464	22.829934072493472
38	30.399608184755174	26.460538516382393	20.784893175501853	22.35496012336058
39	30.341320565326136	24.84895598838329	20.868370739870134	23.94135270642044
40	27.957714053948575	25.265833634124657	21.410751706220193	25.365700605706575
41	26.11221578408327	25.579591935646384	22.51725989518432	25.790932385086023
42	25.9696853361359	23.1393558771649	22.66081069533176	28.230148091367436
43	25.408236538047017	22.80404263541668	24.286678154131852	27.501042672404445
44	26.36724006208843	23.137315172764758	23.542267452167803	26.953177312979008
45	27.55697072737082	22.633516273979872	23.669556389126615	26.13995660952269
46	27.04060874212256	24.08681666694301	22.26587062189191	26.606703969042517
47	25.7443788159578	23.974896784997764	23.722104527430254	26.558619871614187
48	27.516029095342986	23.75379921764495	23.729055676793237	25.001116010218826
49	27.658113139202822	23.188077694718274	24.344455597460854	24.809353568618047
50	25.677481974840667	24.04459959466509	23.766744936183347	26.511173494310896
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	11.5
2	23.0
3	17.5
4	12.0
5	78.5
6	145.0
7	753.0
8	1361.0
9	1881.5
10	2402.0
11	3335.5
12	4269.0
13	3919.0
14	3569.0
15	3563.0
16	3557.0
17	3592.5
18	3628.0
19	4092.0
20	4556.0
21	5508.5
22	6461.0
23	7873.0
24	9285.0
25	10722.0
26	12159.0
27	14331.0
28	16503.0
29	19479.0
30	22455.0
31	24727.0
32	26999.0
33	27251.5
34	27504.0
35	30584.0
36	33664.0
37	37698.5
38	41733.0
39	44051.5
40	46370.0
41	48572.5
42	50775.0
43	56617.0
44	62459.0
45	63183.5
46	63908.0
47	70901.0
48	77894.0
49	83072.5
50	88251.0
51	93720.0
52	99189.0
53	113247.5
54	127306.0
55	130455.5
56	133605.0
57	135661.0
58	137717.0
59	141127.0
60	144537.0
61	123867.5
62	103198.0
63	85871.5
64	68545.0
65	57864.5
66	47184.0
67	40768.0
68	34352.0
69	30177.5
70	26003.0
71	23678.0
72	21353.0
73	15296.5
74	9240.0
75	6242.0
76	3244.0
77	2242.0
78	1240.0
79	1126.5
80	1013.0
81	680.5
82	348.0
83	196.0
84	44.0
85	32.0
86	20.0
87	12.0
88	4.0
89	3.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1568086.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.920526461913532
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.30989139806036	6.677495587629838
2	9.591347576486598	1.90302434875891
3	3.762662153974825	1.1198276839724348
4	1.8671181590045018	0.7409118041569376
5	1.1646650827622507	0.5777045386404811
6	0.7406928261728715	0.44088376693184944
7	0.5739835072729629	0.39859530008223565
8	0.45773520169096626	0.36327793447396467
9	0.36490372836526774	0.32580333839686915
>10	8.853032345675588	24.927651734785705
>50	3.6713144350320115	24.97322959358143
>100	1.5544793373734545	26.276584686687848
>500	0.05747654692809411	3.8902379535180907
>1k	0.028085130885318714	4.998429957365921
>5k	0.0019594277361850267	1.5511966656210643
>10k+	6.531425787283422E-4	0.8351451053964034
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	12889	0.8219574691694206	No Hit
TTCATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	9794	0.6245830904682524	No Hit
TTCATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	8813	0.56202274620142	No Hit
TTCATAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	5333	0.34009614268605165	No Hit
TTCATAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	4729	0.30157784713338426	No Hit
TTCATAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	4288	0.27345438961893676	No Hit
TTCATAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	3638	0.23200258149106617	No Hit
TTCATAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	3617	0.23066336922847344	No Hit
TTCATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	3481	0.22199037552787285	No Hit
TTCATAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	3094	0.1973106066886638	No Hit
TTCATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	2203	0.1404897435472289	No Hit
TTCATAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	2148	0.13698228285948602	No Hit
TTCATAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	2001	0.12760779702133684	No Hit
TTCATAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT	1970	0.12563086463369993	No Hit
TTCATAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA	1901	0.12123059577089522	No Hit
TTCATAGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTG	1876	0.11963629545828482	No Hit
TTCATAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1873	0.11944497942077156	No Hit
TTCATAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT	1852	0.11810576715817883	No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	1841	0.11740427502063024	No Hit
TTCATAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	1729	0.11026180962013563	No Hit
TTCATAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	1720	0.10968786150759588	No Hit
TTCATAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	1719	0.10962408949509146	No Hit
TTCATAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	1697	0.10822110521999431	No Hit
TTCATAGGGGAGTGCAGTGCTTTCATAGGGAAAAAAAAAAAAAAAAAAAA	1693	0.10796601716997664	No Hit
TTCATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	1651	0.10528759264479116	No Hit
TTCATAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAG	1592	0.1015250439070306	No Hit
TTCATAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1576	0.10050469170695994	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.06198639615429256	0.0	0.0	0.0	0.0
7	0.0645372766544692	0.0	0.0	0.0	0.0
8	0.08832423731861645	0.0	0.0	0.0	0.0
9	0.11332286622034761	0.0	0.0	0.0	0.0
10	0.15866476711098754	0.0	0.0	0.0	0.0
11	0.19578007838855777	0.0	0.0	0.0	0.0
12	0.21707993056503277	0.0	0.0	0.0	0.0
13	0.23570135821632232	0.0	0.0	0.0	0.0
14	0.25553445410519576	0.0	0.0	0.0	0.0
15	0.2828288754570859	0.0	0.0	0.0	0.0
16	0.3055317119086581	0.0	0.0	0.0	0.0
17	0.32906358452278767	0.0	0.0	0.0	0.0
18	0.3528505451869349	0.0	0.0	0.0	0.0
19	0.37638241780106446	0.0	0.0	0.0	0.0
20	0.4013810467027956	0.0	0.0	0.0	0.0
21	0.4247853752919164	0.0	0.0	0.0	0.0
22	0.44991154821865637	0.0	0.0	0.0	0.0
23	0.4794379900082011	0.0	0.0	0.0	0.0
24	0.5092195198477635	0.0	0.0	0.0	0.0
25	0.5351747289370609	0.0	0.0	0.0	0.0
26	0.5651475748141365	0.0	0.0	0.0	0.0
27	0.5946740166036811	0.0	0.0	0.0	0.0
28	0.6262411627933672	0.0	0.0	0.0	0.0
29	0.6558313765954163	0.0	0.0	0.0	0.0
30	0.6837635180723506	0.0	0.0	0.0	0.0
31	0.706657670561436	0.0	0.0	0.0	0.0
32	0.7315287554381583	0.0	0.0	0.0	0.0
33	0.7531474676771555	0.0	0.0	0.0	0.0
34	0.775148811991179	0.0	0.0	0.0	0.0
35	0.8011040210804764	0.0	0.0	0.0	0.0
36	0.8267403701072518	0.0	0.0	0.0	0.0
37	0.8518027710214874	0.0	0.0	0.0	0.0
38	0.8775666640732714	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGACG	30	2.528308E-6	44.000004	11
CCGATTA	35	1.4465331E-7	44.0	31
ATTACGC	25	4.4435852E-5	44.0	27
AATATCG	20	7.8573613E-4	44.0	18
TTACGTC	25	4.4435852E-5	44.0	30
CCGAATA	20	7.8573613E-4	44.0	29
TATCGTA	20	7.8573613E-4	44.0	26
CGTTTAT	25	4.4435852E-5	44.0	31
CGATACT	35	1.4465331E-7	44.0	26
GCGTATC	25	4.4435852E-5	44.0	26
TAGGGAT	15890	0.0	41.563248	5
AGGGATA	1990	0.0	41.346733	6
TAGGGGC	18650	0.0	40.850403	5
ATAGGGG	94840	0.0	40.733868	4
TAGGGAG	18480	0.0	40.690475	5
AGGGATC	4250	0.0	40.635292	6
CATAGGG	165340	0.0	40.60034	3
TCATAGG	167965	0.0	40.57619	2
TTCATAG	169400	0.0	40.566235	1
TAGGGGT	16235	0.0	40.436092	5
>>END_MODULE