FastQCFastQC Report
Fri 17 Jun 2016
SRR1527710_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527710_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2354345
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC193610.8223518643189508No Hit
TTCATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT140000.5946452197957394No Hit
TTCATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG132860.5643183135861566No Hit
TTCATAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT78700.33427556284231924No Hit
TTCATAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC72610.30840849578120455No Hit
TTCATAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC61610.26168637136868217No Hit
TTCATAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC55800.237008594747159No Hit
TTCATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA52490.22294948276484544No Hit
TTCATAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG51650.219381611446071No Hit
TTCATAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT45570.19355701904351316No Hit
TTCATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT34150.14505095897160358No Hit
TTCATAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG33290.14139813833571546No Hit
TTCATAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC31460.133625275819814No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC29070.12347383242472959No Hit
TTCATAGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG28910.12279423788782017No Hit
TTCATAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG28850.12253938993647914No Hit
TTCATAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT28530.12118020086266032No Hit
TTCATAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA28520.12113772620410347No Hit
TTCATAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC27830.11820697476368161No Hit
TTCATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT26720.11349228766387254No Hit
TTCATAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT26220.1113685547360306No Hit
TTCATAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG26030.11056153622345068No Hit
TTCATAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC25790.10954214441808656No Hit
TTCATAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT25370.10775820875869933No Hit
TTCATAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA24980.10610169707498264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAAT351.0201802E-746.00000416
TAATACG206.3128094E-446.039
TAAAGCG206.3128094E-446.045
TACGGTA253.418255E-546.024
TACGAAT253.418255E-546.043
TCGTACG453.110472E-1046.026
CGGTATA206.3128094E-446.021
CGCAATA253.418255E-546.031
ACGGTAA800.046.046
TAGGGAT239900.043.1046265
TAGGGGC290050.042.6140335
ATAGGGG1427050.042.3913654
TAGGGAG274000.042.3653265
TCATAGG2527000.042.3520432
CATAGGG2491050.042.352023
TTCATAG2548450.042.3403241
TAGGGAC127100.042.2903255
ATAGGGA795300.042.150764
TAGGGGA400050.042.1249855
TAGGGGT236700.042.122945
Produced by FastQC (version 0.11.5)