FastQCFastQC Report
Fri 17 Jun 2016
SRR1527709_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527709_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2367008
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC180150.7610874149981749No Hit
TTCATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT135880.574058051345834No Hit
TTCATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG124500.5259804783084806No Hit
TTCATAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT75260.3179541429517771No Hit
TTCATAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC69230.2924789438818965No Hit
TTCATAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC60460.2554279495464316No Hit
TTCATAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC50870.21491266611688684No Hit
TTCATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA50480.21326501642579998No Hit
TTCATAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG48590.2052802525382255No Hit
TTCATAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT43260.1827623734267058No Hit
TTCATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT32400.1368816666441347No Hit
TTCATAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG30900.130544552447647No Hit
TTCATAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC29580.12496789195473779No Hit
TTCATAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT27300.11533547837607647No Hit
TTCATAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG27080.1144060349605916No Hit
TTCATAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA26920.11373007611296625No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC26770.11309636469331748No Hit
TTCATAGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG26730.11292737498141113No Hit
TTCATAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC25910.10946308588733117No Hit
TTCATAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC24980.10553407508550879No Hit
TTCATAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT24570.10380193053846881No Hit
TTCATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT24550.10371743568251565No Hit
TTCATAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT24520.1035906933985859No Hit
TTCATAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG24430.10321046654679664No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA206.312816E-446.00000426
ACGAGTA206.312816E-446.00000432
TATCGTT351.0201802E-746.00000421
TATCGTA206.312816E-446.00000426
CGTACGT301.862245E-646.027
TAGGGAT239800.043.103425
CGATACT750.042.9333326
TAGGGGC286300.042.7865875
ATAGGGG1439250.042.5034564
TCATAGG2537950.042.412182
CATAGGG2503250.042.3964423
TTCATAG2560000.042.3909761
TAGGGAG277050.042.3638345
TAGGGAC130550.042.3355035
TAGGGGA411050.042.318215
ATAGGGA799700.042.2323384
TAGGGGG511700.042.098495
TAGGGGT237250.042.093155
ATAGGGC165800.042.0741844
AGGGATT85450.042.0432976
Produced by FastQC (version 0.11.5)