##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527709_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2367008
Sequences flagged as poor quality	0
Sequence length	52
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1732790932688	33.0	31.0	34.0	31.0	34.0
2	32.747284757803946	34.0	31.0	34.0	31.0	34.0
3	32.74874187159486	34.0	31.0	34.0	31.0	34.0
4	36.18459760169801	37.0	35.0	37.0	35.0	37.0
5	36.22808076694291	37.0	37.0	37.0	35.0	37.0
6	36.11859486744447	37.0	37.0	37.0	35.0	37.0
7	36.38539624707648	37.0	36.0	37.0	35.0	37.0
8	36.569657981722074	37.0	37.0	37.0	35.0	37.0
9	38.63980687855723	39.0	39.0	39.0	38.0	39.0
10	37.96638625640471	39.0	38.0	39.0	35.0	39.0
11	37.686124001270805	39.0	38.0	39.0	35.0	39.0
12	37.272058649569416	39.0	37.0	39.0	35.0	39.0
13	36.98898989779502	39.0	37.0	39.0	33.0	39.0
14	37.93391868553042	40.0	38.0	40.0	34.0	40.0
15	38.013883349781665	40.0	38.0	40.0	34.0	40.0
16	38.08332333477538	40.0	38.0	40.0	34.0	40.0
17	38.03844980667577	40.0	38.0	40.0	34.0	40.0
18	38.012834346145006	40.0	38.0	40.0	34.0	40.0
19	38.006164322216065	40.0	38.0	40.0	34.0	40.0
20	37.98938829104084	40.0	38.0	40.0	34.0	40.0
21	38.028063698982	40.0	38.0	40.0	34.0	40.0
22	37.92063229190607	40.0	38.0	40.0	34.0	40.0
23	37.92548567643202	40.0	37.0	40.0	34.0	40.0
24	37.901255086590325	40.0	37.0	40.0	34.0	40.0
25	37.818301416809746	40.0	37.0	40.0	34.0	40.0
26	37.63224289905231	40.0	37.0	40.0	34.0	40.0
27	37.52071475888548	40.0	36.0	40.0	33.0	40.0
28	37.44814508442726	40.0	36.0	40.0	33.0	40.0
29	37.358592366396735	40.0	36.0	40.0	33.0	40.0
30	37.213996741878354	40.0	36.0	40.0	33.0	40.0
31	37.112455471210914	40.0	35.0	40.0	32.0	40.0
32	37.03159389406373	39.0	35.0	40.0	32.0	40.0
33	36.69768416498803	39.0	35.0	40.0	31.0	40.0
34	36.60879895631954	39.0	35.0	40.0	31.0	40.0
35	36.53573371953115	39.0	35.0	40.0	31.0	40.0
36	36.4070476314402	39.0	35.0	40.0	31.0	40.0
37	36.39084405291406	39.0	35.0	40.0	31.0	40.0
38	36.016366653598126	39.0	35.0	40.0	30.0	40.0
39	36.06920677919128	38.0	35.0	40.0	30.0	40.0
40	35.88521078086766	38.0	35.0	40.0	30.0	40.0
41	35.56825874690749	38.0	35.0	40.0	29.0	40.0
42	35.625898602793065	38.0	35.0	40.0	29.0	40.0
43	35.56950884830132	38.0	35.0	40.0	29.0	40.0
44	35.44612776974138	38.0	34.0	40.0	29.0	40.0
45	35.304762806040365	38.0	34.0	40.0	28.0	40.0
46	35.01512373426706	37.0	34.0	40.0	27.0	40.0
47	34.71699081709905	37.0	34.0	40.0	26.0	40.0
48	34.69627225594506	37.0	33.0	40.0	26.0	40.0
49	34.61056574375752	36.0	33.0	40.0	27.0	40.0
50	34.459680744636266	36.0	33.0	40.0	26.0	40.0
51	34.71180452284065	36.0	34.0	40.0	27.0	40.0
52	34.41520138503968	36.0	34.0	40.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	3.0
14	3.0
15	16.0
16	60.0
17	161.0
18	364.0
19	715.0
20	1333.0
21	2306.0
22	3750.0
23	5913.0
24	8920.0
25	12432.0
26	16940.0
27	22152.0
28	27968.0
29	33637.0
30	39656.0
31	49375.0
32	64987.0
33	90926.0
34	163389.0
35	179894.0
36	237874.0
37	382886.0
38	709883.0
39	311464.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.68242608390001	0.26713048709594556	0.028939488163960577	0.021503940840081656
8	99.1655288026065	0.7581723424677906	0.055259635793372903	0.021039219132339224
9	98.34778758669172	0.8652695723884329	0.31233523503089133	0.4746076058889535
10	57.32798537225053	31.695245643445226	4.123348970514675	6.8534200137895604
11	34.60710736930336	25.16759554678311	21.945722194432804	18.279574889480728
12	32.12950695561654	18.006867741891874	26.582377414862986	23.281247887628602
13	24.521843610161014	18.076026781489542	29.83914714272195	27.562982465627496
14	25.811868823426032	19.517889250902403	29.7244876231935	24.945754302478065
15	26.082125620192244	18.839522299882383	28.470457218564533	26.607894861360844
16	29.897490840757612	20.89752125890576	25.396154132136434	23.80883376820019
17	31.00107815436196	20.640445659668238	23.125861847530725	25.232614338439074
18	28.35262914193784	21.679690140464245	25.087156443915692	24.880524273682216
19	29.108139896443102	21.374367978477473	23.528353093863647	25.989139031215778
20	27.982203693439146	22.522146101745324	24.300382592707756	25.195267612107774
21	29.494872852140762	21.15231549703254	26.209501615541647	23.143310035285054
22	27.971346104449164	20.47242763860536	25.826951155213674	25.729275101731808
23	28.6328985791345	21.707531195500817	25.940807973610568	23.718762251754114
24	29.599181751814953	21.342766902350984	23.726155551650017	25.33189579418405
25	27.45820884424556	22.912174356825158	22.606767699982424	27.022849098946857
26	27.237550527923858	21.642216671849017	24.20262204436994	26.917610755857186
27	26.930369479106115	21.748257716070245	23.466502859305926	27.85486994551772
28	29.71029248739337	22.375716516378482	21.526205234625316	26.387785761602835
29	31.027271559707447	22.364689937676594	21.591899985128908	25.016138517487057
30	30.141216252754532	21.407785694006947	23.1625326150144	25.288465438224122
31	31.078390947559114	22.366506577079587	22.408627262772242	24.14647521258906
32	32.608001324879346	22.785263083183498	21.533514039665267	23.073221552271896
33	32.15878442320432	22.175548202625425	22.14779164244481	23.51787573172545
34	29.248443604753344	23.628352755884222	24.955640200624586	22.16756343873785
35	27.7590950262948	26.414359393800108	24.80629554272736	21.02025003717774
36	28.87700421798321	26.166831713285294	22.24213014911652	22.714033919614973
37	29.486423366545445	26.496699630926468	21.148048506806905	22.86882849572118
38	30.432385526369153	26.37435107950628	20.71970183455231	22.473561559572254
39	30.36762021928105	24.75568312401141	20.874580905514474	24.002115751193067
40	27.978105692925414	25.141359894009653	21.435542254187563	25.444992158877366
41	26.290236450404898	25.48419777203964	22.514921791561328	25.710643985994132
42	25.940343251902824	22.98695230434371	22.807316240587273	28.265388203166193
43	25.505025754032097	22.579264624369667	24.286525436331434	27.6291841852668
44	26.353016128378105	22.952605145398746	23.61158052697752	27.082798199245627
45	27.519721099379467	22.451804134164313	23.721339344860684	26.307135421595536
46	27.165898890075574	23.94541970284849	22.232075261258093	26.65660614581784
47	25.91723390879963	23.872627384444836	23.73143648014709	26.47870222660844
48	27.624579215617352	23.66210845083751	23.64533622193081	25.067976111614325
49	27.721283578255758	23.13570549824927	24.235068069055956	24.90794285443902
50	25.714615244223932	24.000003379794236	23.767051061931348	26.51833031405048
51	25.135318511809	23.850109505333315	24.12987197339426	26.884700009463426
52	25.787027335775797	23.614199867512063	24.616815828252374	25.981956968459762
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	11.5
2	23.0
3	22.5
4	22.0
5	106.0
6	190.0
7	1024.0
8	1858.0
9	2480.5
10	3103.0
11	4327.0
12	5551.0
13	5186.0
14	4560.5
15	4300.0
16	4491.0
17	4682.0
18	4936.5
19	5191.0
20	6197.0
21	7203.0
22	8926.0
23	10649.0
24	12318.0
25	13987.0
26	17977.0
27	21967.0
28	24857.5
29	27748.0
30	31072.5
31	34397.0
32	36806.5
33	39216.0
34	40324.5
35	41433.0
36	47855.0
37	54277.0
38	58126.0
39	65445.0
40	68915.0
41	72696.5
42	76478.0
43	80758.0
44	85038.0
45	89509.0
46	93980.0
47	102090.0
48	110200.0
49	120376.5
50	130553.0
51	138360.0
52	146167.0
53	160880.0
54	175593.0
55	194164.5
56	212736.0
57	205761.0
58	198786.0
59	200844.5
60	202903.0
61	180180.5
62	157458.0
63	139632.0
64	101576.0
65	81346.0
66	66274.5
67	51203.0
68	47526.5
69	43850.0
70	39790.5
71	35731.0
72	27370.0
73	19009.0
74	13072.5
75	7136.0
76	5062.0
77	2988.0
78	2379.0
79	1770.0
80	1209.0
81	648.0
82	369.0
83	90.0
84	52.5
85	15.0
86	14.0
87	13.0
88	7.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2367008.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.161823041725885
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.18162690979784	7.23335096479519
2	9.34086353600575	1.8984040461960072
3	3.9729346995266313	1.211167781187661
4	2.046997436763298	0.8320490287702045
5	1.1809663216225397	0.6000385389283093
6	0.7384154048446621	0.4502188005189501
7	0.5118869040773127	0.36411928956273953
8	0.3888454585242347	0.31611029920816286
9	0.30989791494737084	0.2834214995425502
>10	4.821019936126585	13.605690165435858
>50	3.4356074414300357	24.910963983669124
>100	1.9664990515218292	34.646358440467104
>500	0.06701501525433982	4.498235488862215
>1k	0.033942670063886404	5.847356794070451
>5k	0.002175812183582462	1.3544719780565817
>10k+	0.0013054873101494772	1.9480429007289124
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	18015	0.7610874149981749	No Hit
TTCATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	13588	0.574058051345834	No Hit
TTCATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	12450	0.5259804783084806	No Hit
TTCATAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	7526	0.3179541429517771	No Hit
TTCATAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	6923	0.2924789438818965	No Hit
TTCATAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	6046	0.2554279495464316	No Hit
TTCATAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	5087	0.21491266611688684	No Hit
TTCATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	5048	0.21326501642579998	No Hit
TTCATAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	4859	0.2052802525382255	No Hit
TTCATAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	4326	0.1827623734267058	No Hit
TTCATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	3240	0.1368816666441347	No Hit
TTCATAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	3090	0.130544552447647	No Hit
TTCATAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2958	0.12496789195473779	No Hit
TTCATAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	2730	0.11533547837607647	No Hit
TTCATAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	2708	0.1144060349605916	No Hit
TTCATAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA	2692	0.11373007611296625	No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	2677	0.11309636469331748	No Hit
TTCATAGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG	2673	0.11292737498141113	No Hit
TTCATAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	2591	0.10946308588733117	No Hit
TTCATAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2498	0.10553407508550879	No Hit
TTCATAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	2457	0.10380193053846881	No Hit
TTCATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	2455	0.10371743568251565	No Hit
TTCATAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2452	0.1035906933985859	No Hit
TTCATAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	2443	0.10321046654679664	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0519220889832227	0.0	0.0	0.0	0.0
7	0.05416120266598169	0.0	0.0	0.0	0.0
8	0.07452446295069556	0.0	0.0	0.0	0.0
9	0.0977605483378172	0.0	0.0	0.0	0.0
10	0.1393742648947532	0.0	0.0	0.0	0.0
11	0.1738481661236464	0.0	0.0	0.0	0.0
12	0.19416917898038366	0.0	0.0	0.0	0.0
13	0.21068792331922834	0.0	0.0	0.0	0.0
14	0.22969926590869147	0.0	0.0	0.0	0.0
15	0.25314658843569604	0.0	0.0	0.0	0.0
16	0.27481951898768403	0.0	0.0	0.0	0.0
17	0.29383086157714716	0.0	0.0	0.0	0.0
18	0.31195500817910204	0.0	0.0	0.0	0.0
19	0.33062837134475254	0.0	0.0	0.0	0.0
20	0.3541179412997337	0.0	0.0	0.0	0.0
21	0.3762133461314875	0.0	0.0	0.0	0.0
22	0.4006746069299301	0.0	0.0	0.0	0.0
23	0.426023063715881	0.0	0.0	0.0	0.0
24	0.4507800565101597	0.0	0.0	0.0	0.0
25	0.4733401830496559	0.0	0.0	0.0	0.0
26	0.49632278386891804	0.0	0.0	0.0	0.0
27	0.5215444983709392	0.0	0.0	0.0	0.0
28	0.5473154294366559	0.0	0.0	0.0	0.0
29	0.56755194743744	0.0	0.0	0.0	0.0
30	0.5890136408495451	0.0	0.0	0.0	0.0
31	0.6085319525747273	0.0	0.0	0.0	0.0
32	0.631049831686247	0.0	0.0	0.0	0.0
33	0.6506526382673823	0.0	0.0	0.0	0.0
34	0.6684388054455245	0.0	0.0	0.0	0.0
35	0.6914636536927632	0.0	0.0	0.0	0.0
36	0.7135168110965404	0.0	0.0	0.0	0.0
37	0.7343870405169733	0.0	0.0	0.0	0.0
38	0.7561867133528911	0.0	0.0	0.0	0.0
39	0.7768879530614177	0.0	0.0	0.0	0.0
40	0.7999128013086563	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATA	20	6.312816E-4	46.000004	26
ACGAGTA	20	6.312816E-4	46.000004	32
TATCGTT	35	1.0201802E-7	46.000004	21
TATCGTA	20	6.312816E-4	46.000004	26
CGTACGT	30	1.862245E-6	46.0	27
TAGGGAT	23980	0.0	43.10342	5
CGATACT	75	0.0	42.93333	26
TAGGGGC	28630	0.0	42.786587	5
ATAGGGG	143925	0.0	42.503456	4
TCATAGG	253795	0.0	42.41218	2
CATAGGG	250325	0.0	42.396442	3
TTCATAG	256000	0.0	42.390976	1
TAGGGAG	27705	0.0	42.363834	5
TAGGGAC	13055	0.0	42.335503	5
TAGGGGA	41105	0.0	42.31821	5
ATAGGGA	79970	0.0	42.232338	4
TAGGGGG	51170	0.0	42.09849	5
TAGGGGT	23725	0.0	42.09315	5
ATAGGGC	16580	0.0	42.074184	4
AGGGATT	8545	0.0	42.043297	6
>>END_MODULE