FastQCFastQC Report
Fri 17 Jun 2016
SRR1527708_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527708_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320998
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGA67242.094717101041128No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGG22590.7037427024467443No Hit
CTCCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT16440.5121527236929826No Hit
CTCCATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC16440.5121527236929826No Hit
CTCCATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC16000.49844547318051824No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC10000.3115284207378239No Hit
CTCCATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC9460.2947058860179814No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC8910.2775718228774011No Hit
CTCCATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG7440.231777145028941No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG7350.22897338924230057Illumina Single End Adapter 2 (100% over 23bp)
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG6180.19252456401597517No Hit
CTCCATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC6010.18722858086343216No Hit
CTCCATGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC5540.17258674508875443No Hit
CTCCATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG5210.16230630720440625No Hit
CTCCATGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCT4790.14922211353341766No Hit
CTCCATGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC4730.1473529430089907No Hit
CTCCATGGGGGAAGACGCGCCTGCGCAGAAGTGACAGCACGAGCGGGATT4670.14548377248456376No Hit
CTCCATGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA4540.14143390301497205Illumina Single End Adapter 2 (100% over 34bp)
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGC4480.1395647324905451No Hit
CTCCATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG4370.13613791986242904No Hit
CTCCATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAA3830.11931538514258655No Hit
CTCCATGGGAAGTATTACAGCTCCTGAACTGCCTTCTACTAAATCAGACT3640.11339634514856792No Hit
CTCCATGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTG3610.11246175988635443No Hit
CTCCATGGGGCTCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAG3580.11152717462414095No Hit
CTCCATGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGCT3580.11152717462414095No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCG3560.11090411778266532No Hit
CTCCATGGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT3550.11059258936192749No Hit
CTCCATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT3530.10996953252045184No Hit
CTCCATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT3370.10498507778864666No Hit
CTCCATGGGGCTCTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTG3360.10467354936790883No Hit
CTCCATGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTC3360.10467354936790883No Hit
CTCCATGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAAC3350.10436202094717101No Hit
CTCCATGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGG3290.10249285042274406No Hit
CTCCATGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA3270.10186979358126841No Hit
CTCCATGGGGCTCAGCCCGTAGCCCGTCGGTTCCGGAGTAAGTTCCAGGT3250.10124673673979279No Hit
CTCCATGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGA3250.10124673673979279No Hit
CTCCATGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG3250.10124673673979279No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTTA254.4356482E-544.018
TATTTCC207.847993E-444.044
TTCTATA302.521996E-644.039
TGTTTAC207.847993E-444.015
AAACTAC254.4356482E-544.044
TAATAGT207.847993E-444.013
CCTTATT207.847993E-444.042
GATACGT207.847993E-444.036
AATCCGA207.847993E-444.033
CCCCTAT254.4356482E-544.026
AGATCGA254.4356482E-544.016
CGAACAG254.4356482E-544.020
GCGACTC254.4356482E-544.023
TTGTGTA254.4356482E-544.021
TCACCCG302.521996E-644.036
AATCAGT254.4356482E-544.029
GGTGTAC207.847993E-444.026
GATAACT207.847993E-444.09
CATGCTA207.847993E-444.026
AAATACG408.28004E-944.029
Produced by FastQC (version 0.11.5)