SRR1527707_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527707_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	483698
Sequences flagged as poor quality	0
Sequence length	52
%GC	58

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGAAG	8374	1.7312455292351838	No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGGAG	3361	0.6948550541867032	No Hit
CTCCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2598	0.5371119996361366	No Hit
CTCCATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	2498	0.5164379426832445	No Hit
CTCCATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2339	0.48356619212814606	No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	1470	0.3039086372075138	No Hit
CTCCATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1448	0.29936034467787753	No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	1334	0.27579191975158057	No Hit
CTCCATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1145	0.23671795211061447	No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC	1026	0.2121158243366729	Illumina Single End Adapter 2 (100% over 25bp)
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	975	0.20157205529069794	No Hit
CTCCATGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	875	0.18089799833780584	No Hit
CTCCATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	831	0.1718014132785333	No Hit
CTCCATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	787	0.16270482821926077	No Hit
CTCCATGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	689	0.14244425240542652	No Hit
CTCCATGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGA	677	0.1399633655710795	No Hit
CTCCATGGGGGAAGACGCGCCTGCGCAGAAGTGACAGCACGAGCGGGATTGG	666	0.13768921930626135	No Hit
CTCCATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	616	0.1273521908298153	No Hit
CTCCATGGGGCTCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTG	604	0.12487130399546824	No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA	568	0.1174286434924271	No Hit
CTCCATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACG	542	0.11205338868467515	No Hit
CTCCATGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	542	0.11205338868467515	Illumina Single End Adapter 2 (100% over 34bp)
CTCCATGGGAAGTATTACAGCTCCTGAACTGCCTTCTACTAAATCAGACTTG	533	0.11019272355891487	No Hit
CTCCATGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCGG	520	0.10750509615503889	No Hit
CTCCATGGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	517	0.10688487444645213	No Hit
CTCCATGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGAT	512	0.10585117159880753	No Hit
CTCCATGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGC	510	0.10543769045974968	No Hit
CTCCATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	510	0.10543769045974968	No Hit
CTCCATGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT	503	0.10399050647304724	No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGCAG	498	0.10295680362540263	No Hit
CTCCATGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC	497	0.10275006305587371	No Hit
CTCCATGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	495	0.10233658191681587	No Hit
CTCCATGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	490	0.10130287906917126	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAT	20	6.3078094E-4	46.000004	27
CTTAATC	20	6.3078094E-4	46.000004	33
GTTGACT	20	6.3078094E-4	46.000004	45
CTTCGAA	20	6.3078094E-4	46.000004	40
AACCGTA	20	6.3078094E-4	46.000004	27
AACCGCG	20	6.3078094E-4	46.000004	17
ATAGGGC	40	5.6006684E-9	46.000004	5
CGACGGC	20	6.3078094E-4	46.000004	22
CGACGAC	20	6.3078094E-4	46.000004	33
CTATCCG	20	6.3078094E-4	46.000004	11
CGGTTAG	20	6.3078094E-4	46.000004	12
ACCGATG	20	6.3078094E-4	46.000004	30
CCGTGTA	20	6.3078094E-4	46.000004	21
ACATTAT	20	6.3078094E-4	46.000004	18
TATATGC	20	6.3078094E-4	46.000004	19
CGATTTC	20	6.3078094E-4	46.000004	42
GAATATA	20	6.3078094E-4	46.000004	10
ATAGAAC	20	6.3078094E-4	46.000004	46
TACGGCA	20	6.3078094E-4	46.000004	23
AGGCGAT	20	6.3078094E-4	46.000004	42

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content