##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527707_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	483698
Sequences flagged as poor quality	0
Sequence length	52
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20198140161836	33.0	31.0	34.0	31.0	34.0
2	32.39047504848067	34.0	31.0	34.0	31.0	34.0
3	32.72441275341225	34.0	31.0	34.0	31.0	34.0
4	36.32378674296772	37.0	37.0	37.0	35.0	37.0
5	35.83932122936212	37.0	35.0	37.0	35.0	37.0
6	36.161900607403794	37.0	35.0	37.0	35.0	37.0
7	36.4187220124954	37.0	36.0	37.0	35.0	37.0
8	36.54014488379113	37.0	37.0	37.0	35.0	37.0
9	38.60255159210912	39.0	39.0	39.0	37.0	39.0
10	37.835074778064	39.0	38.0	39.0	35.0	39.0
11	37.57944006384149	39.0	37.0	39.0	35.0	39.0
12	37.32098954306199	39.0	37.0	39.0	34.0	39.0
13	37.16486113235945	39.0	37.0	39.0	34.0	39.0
14	37.956332256904105	40.0	38.0	40.0	33.0	40.0
15	38.04803823873574	40.0	38.0	40.0	33.0	40.0
16	37.788812440820514	40.0	37.0	40.0	33.0	40.0
17	37.906654565451994	40.0	37.0	40.0	33.0	40.0
18	37.9474672212827	40.0	38.0	40.0	34.0	40.0
19	37.473564083374335	40.0	37.0	40.0	32.0	40.0
20	37.73249217486944	40.0	37.0	40.0	33.0	40.0
21	37.838903613411674	40.0	37.0	40.0	33.0	40.0
22	37.84647445306782	40.0	37.0	40.0	33.0	40.0
23	37.803939648292946	40.0	37.0	40.0	33.0	40.0
24	37.75854768884717	40.0	37.0	40.0	33.0	40.0
25	37.66473295320634	40.0	37.0	40.0	33.0	40.0
26	37.32755148873884	40.0	37.0	40.0	32.0	40.0
27	37.2492319587842	40.0	36.0	40.0	32.0	40.0
28	37.12168336441333	40.0	36.0	40.0	32.0	40.0
29	37.08071978796687	40.0	36.0	40.0	31.0	40.0
30	36.73364371984172	39.0	35.0	40.0	31.0	40.0
31	36.65874574631278	39.0	35.0	40.0	30.0	40.0
32	35.96547845970006	39.0	35.0	40.0	29.0	40.0
33	36.203108137722296	39.0	35.0	40.0	30.0	40.0
34	36.01721528722467	39.0	35.0	40.0	29.0	40.0
35	36.012580163655834	39.0	35.0	40.0	29.0	40.0
36	35.932794843063235	39.0	35.0	40.0	29.0	40.0
37	35.33559369689352	38.0	35.0	40.0	25.0	40.0
38	35.37680536202341	38.0	34.0	40.0	26.0	40.0
39	35.191348734127494	38.0	34.0	40.0	26.0	40.0
40	35.00005168514238	38.0	34.0	40.0	25.0	40.0
41	34.74079901095312	38.0	34.0	40.0	24.0	40.0
42	34.640742777518206	38.0	33.0	40.0	24.0	40.0
43	34.364239670207446	37.0	33.0	40.0	23.0	40.0
44	34.33733031767756	37.0	33.0	40.0	24.0	40.0
45	34.09756500957209	37.0	33.0	40.0	23.0	40.0
46	33.997374394766986	36.0	33.0	40.0	23.0	40.0
47	33.86914148911097	36.0	33.0	40.0	23.0	40.0
48	33.70380071863022	36.0	32.0	40.0	23.0	40.0
49	33.95153174087964	36.0	33.0	40.0	23.0	40.0
50	33.768080496508155	36.0	33.0	40.0	23.0	40.0
51	33.781512431310446	36.0	33.0	40.0	23.0	40.0
52	33.26546522830361	35.0	33.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	1.0
15	4.0
16	7.0
17	29.0
18	64.0
19	127.0
20	315.0
21	567.0
22	1026.0
23	1857.0
24	2869.0
25	3967.0
26	5319.0
27	7286.0
28	8919.0
29	10662.0
30	12051.0
31	13573.0
32	15970.0
33	19618.0
34	29410.0
35	33539.0
36	45463.0
37	74442.0
38	136969.0
39	59643.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.43187691493452	0.3239624724518191	0.13644877588908783	0.1077118367245678
8	99.35207505509636	0.40231714830328014	0.21501019231007779	0.0305976042902803
9	98.2958374853731	1.054376904597497	0.2784795471554565	0.371306062873942
10	56.02958871031098	33.43718601276003	2.8280042505861096	7.705221026342883
11	29.217610988674753	31.256486485368967	18.803468279794416	20.72243424616186
12	34.894293546799865	14.805105665105087	27.01127563066211	23.289325157432945
13	20.388548226372656	14.161315531592026	26.75739821128059	38.692738030754725
14	21.430313956228886	24.72286426654648	30.21761512348614	23.629206653738493
15	30.40616252289652	15.50016745986132	25.102026471062523	28.991643546179642
16	26.98791394630534	17.116051751299363	30.606494134770042	25.28954016762525
17	36.903398401481915	19.84957556161076	19.346369015377363	23.900657021529963
18	35.1891055989481	17.506171206000438	22.638092363416842	24.666630831634613
19	29.826048484798367	17.117912416425124	27.465898143056204	25.590140955720305
20	29.580440688198006	26.557893561685187	20.685014203077127	23.176651547039683
21	31.984006549541245	15.713316987045637	29.79710480506432	22.505571658348806
22	28.382999309486497	17.374890944349573	20.625679659622325	33.616430086541605
23	26.253157962199552	28.10886131429115	21.04660345918321	24.591377264326088
24	26.99328920111309	28.416491281750183	20.57358103610104	24.016638481035688
25	27.040839532104744	19.47144705994236	19.65358550169734	33.83412790625556
26	34.36503768880583	19.35484537872805	19.62836315221481	26.651753780251315
27	26.612679812610345	17.686448982629656	20.587846135398532	35.113025069361456
28	28.08901421961637	27.83120872941381	19.620093529433653	24.459683521536167
29	37.101869348229684	19.384616020740214	18.79581887872185	24.71769575230826
30	28.023477459075703	27.017684588317504	19.660614681061322	25.298223271545467
31	36.526303602661166	20.235353464351725	19.028402019441884	24.20994091354523
32	31.09150751088489	18.616368064370743	26.27693312769538	24.015191297048986
33	36.91849046305753	19.109444322697218	19.55827809914451	24.413787115100742
34	28.537434514924602	21.413361229527514	20.73049712837349	29.31870712717439
35	29.7119276904184	27.786552766395562	21.181191569946538	21.3203279732395
36	36.77873383805598	21.685431819027574	18.08483806011189	23.45099628280456
37	30.352823455958056	22.539683852321076	24.238057631001162	22.869435060719706
38	37.31336495085777	20.81836187042328	18.514651704162517	23.353621474556437
39	29.52234658816038	20.740834156849935	19.853917113570866	29.88290214141882
40	28.048079586849646	21.123717691617497	25.04889414469359	25.779308576839266
41	26.82665630207278	20.760267770385653	21.35836823803282	31.054707689508742
42	25.756153633052026	19.82207906586341	28.07805696943134	26.34371033165322
43	26.154749451103786	18.56571662483616	23.27299265244016	32.006541271619895
44	26.39601569574404	19.645109138346655	21.929592431641233	32.02928273426808
45	27.45907570426175	25.533494039669378	22.244665059603307	24.762765196465566
46	27.571749314655015	20.402193103961565	26.40531902137284	25.620738560010587
47	32.330710484641244	20.58681243255089	21.993888748764725	25.088588334043145
48	33.376197544749	21.043709091209806	22.426390020219227	23.15370334382197
49	32.40823819821459	20.909741202155065	22.691844911494364	23.990175688135988
50	27.647623103672125	23.56801144515793	22.11545220364773	26.668913247522212
51	25.253360567957696	26.35197995443438	22.645535023919884	25.749124453688044
52	31.720619063961397	22.26906044680772	22.097879255237775	23.91244123399311
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	6.0
6	12.0
7	8.5
8	5.0
9	5.0
10	5.0
11	57.0
12	109.0
13	245.0
14	357.0
15	333.0
16	321.5
17	310.0
18	250.5
19	191.0
20	482.5
21	774.0
22	602.0
23	430.0
24	676.0
25	922.0
26	1055.0
27	1188.0
28	1325.0
29	1462.0
30	1531.5
31	1601.0
32	2016.0
33	2431.0
34	2436.0
35	2441.0
36	3252.0
37	4063.0
38	4797.0
39	6148.5
40	6766.0
41	6825.5
42	6885.0
43	8570.0
44	10255.0
45	10757.0
46	11259.0
47	12452.5
48	13646.0
49	16015.5
50	18385.0
51	19736.0
52	21087.0
53	24813.0
54	28539.0
55	37993.0
56	47447.0
57	49061.5
58	50676.0
59	50262.5
60	49849.0
61	45122.5
62	40396.0
63	40062.5
64	35241.0
65	30753.0
66	28728.0
67	26703.0
68	22418.5
69	18134.0
70	16653.0
71	15172.0
72	12013.5
73	8855.0
74	9519.5
75	10184.0
76	7123.0
77	4062.0
78	2961.0
79	1860.0
80	1229.5
81	599.0
82	385.5
83	172.0
84	115.0
85	58.0
86	48.0
87	38.0
88	19.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	483698.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.879524000512715
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.64920222158014	9.356871436309433
2	9.778805098076985	2.5189270991403725
3	3.860477061863944	1.4916332091511646
4	1.9968538315836786	1.0287410739759106
5	1.089922629939966	0.7018842335506866
6	0.691836013997239	0.5346311128017895
7	0.4943978939933866	0.4457326679043535
8	0.361167292689974	0.3721330251520577
9	0.2102796237439404	0.2437471314745978
>10	4.3211660085396	15.16462751551588
>50	2.929468040707567	26.89798179856026
>100	1.5698738322257537	33.475846499261934
>500	0.030498571382708915	2.476131801247886
>1k	0.014446691707598961	3.5598658667184897
>5k	0.0016051879675109955	1.7312455292351838
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGAAG	8374	1.7312455292351838	No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGGAG	3361	0.6948550541867032	No Hit
CTCCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2598	0.5371119996361366	No Hit
CTCCATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	2498	0.5164379426832445	No Hit
CTCCATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2339	0.48356619212814606	No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	1470	0.3039086372075138	No Hit
CTCCATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1448	0.29936034467787753	No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	1334	0.27579191975158057	No Hit
CTCCATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1145	0.23671795211061447	No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC	1026	0.2121158243366729	Illumina Single End Adapter 2 (100% over 25bp)
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	975	0.20157205529069794	No Hit
CTCCATGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	875	0.18089799833780584	No Hit
CTCCATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	831	0.1718014132785333	No Hit
CTCCATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	787	0.16270482821926077	No Hit
CTCCATGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	689	0.14244425240542652	No Hit
CTCCATGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGA	677	0.1399633655710795	No Hit
CTCCATGGGGGAAGACGCGCCTGCGCAGAAGTGACAGCACGAGCGGGATTGG	666	0.13768921930626135	No Hit
CTCCATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	616	0.1273521908298153	No Hit
CTCCATGGGGCTCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTG	604	0.12487130399546824	No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA	568	0.1174286434924271	No Hit
CTCCATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACG	542	0.11205338868467515	No Hit
CTCCATGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	542	0.11205338868467515	Illumina Single End Adapter 2 (100% over 34bp)
CTCCATGGGAAGTATTACAGCTCCTGAACTGCCTTCTACTAAATCAGACTTG	533	0.11019272355891487	No Hit
CTCCATGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCGG	520	0.10750509615503889	No Hit
CTCCATGGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	517	0.10688487444645213	No Hit
CTCCATGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGAT	512	0.10585117159880753	No Hit
CTCCATGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGC	510	0.10543769045974968	No Hit
CTCCATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	510	0.10543769045974968	No Hit
CTCCATGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT	503	0.10399050647304724	No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGCAG	498	0.10295680362540263	No Hit
CTCCATGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC	497	0.10275006305587371	No Hit
CTCCATGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	495	0.10233658191681587	No Hit
CTCCATGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	490	0.10130287906917126	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.007442660503041154	0.0	0.0	0.0	0.0
8	0.012404434171735257	0.0	0.0	0.0	0.0
9	0.03514589681991656	0.0	0.0	0.0	0.0
10	0.09551414312236148	0.0	0.0	0.0	0.0
11	0.27992673114215894	0.0	0.0	0.0	0.0
12	0.29956708524740644	0.0	0.0	0.0	0.0
13	0.32334225074323236	0.0	0.0	0.0	0.0
14	0.4455259273348246	0.0	0.0	0.0	0.0
15	0.48211900814144365	0.0	0.0	0.0	0.0
16	0.5644017548139542	0.0	0.0	0.0	0.0
17	0.6253902228249858	0.0	0.0	0.0	0.0
18	0.6578484922410264	0.0	0.0	0.0	0.0
19	0.69547527589529	0.0	0.0	0.0	0.0
20	0.7436458285955286	0.0	0.0	0.0	0.0
21	0.7628727015617183	0.0	0.0	0.0	0.0
22	0.7891287538918912	0.0	0.0	0.0	0.0
23	0.8319240517843779	0.0	0.0	0.0	0.0
24	0.8848496375837817	0.0	0.0	0.0	0.0
25	0.9166876852912355	0.0	0.0	0.0	0.0
26	1.0510690554850342	0.0	0.0	0.0	0.0
27	1.1104035989398344	0.0	0.0	0.0	0.0
28	1.3890898866648198	0.0	0.0	0.0	0.0
29	1.4343660713916535	0.0	0.0	0.0	0.0
30	1.5495205686192624	0.0	0.0	0.0	0.0
31	1.581978838035303	0.0	0.0	0.0	0.0
32	1.6518571505360784	0.0	0.0	0.0	0.0
33	1.6938254861504491	0.0	0.0	0.0	0.0
34	1.736620784042936	0.0	0.0	0.0	0.0
35	1.9371591364859893	0.0	0.0	0.0	0.0
36	1.9718915521668479	0.0	0.0	0.0	0.0
37	2.0225429917014335	0.0	0.0	0.0	0.0
38	2.071747247249317	0.0	0.0	0.0	0.0
39	2.0944887098974982	0.0	0.0	0.0	0.0
40	2.1201245405190843	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAT	20	6.3078094E-4	46.000004	27
CTTAATC	20	6.3078094E-4	46.000004	33
GTTGACT	20	6.3078094E-4	46.000004	45
CTTCGAA	20	6.3078094E-4	46.000004	40
AACCGTA	20	6.3078094E-4	46.000004	27
AACCGCG	20	6.3078094E-4	46.000004	17
ATAGGGC	40	5.6006684E-9	46.000004	5
CGACGGC	20	6.3078094E-4	46.000004	22
CGACGAC	20	6.3078094E-4	46.000004	33
CTATCCG	20	6.3078094E-4	46.000004	11
CGGTTAG	20	6.3078094E-4	46.000004	12
ACCGATG	20	6.3078094E-4	46.000004	30
CCGTGTA	20	6.3078094E-4	46.000004	21
ACATTAT	20	6.3078094E-4	46.000004	18
TATATGC	20	6.3078094E-4	46.000004	19
CGATTTC	20	6.3078094E-4	46.000004	42
GAATATA	20	6.3078094E-4	46.000004	10
ATAGAAC	20	6.3078094E-4	46.000004	46
TACGGCA	20	6.3078094E-4	46.000004	23
AGGCGAT	20	6.3078094E-4	46.000004	42
>>END_MODULE