FastQCFastQC Report
Fri 17 Jun 2016
SRR1527706_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527706_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486308
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGAAG77701.59775286443982No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGGAG36100.7423279074166989No Hit
CTCCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG24250.49865517326467995No Hit
CTCCATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT23510.4834384793176341No Hit
CTCCATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC22410.4608190693963497No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA14350.2950804839731199No Hit
CTCCATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC14230.2926129119817071No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC12630.2597119520962024No Hit
CTCCATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG10970.22557720621499133No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC10230.2103605122679454Illumina Single End Adapter 2 (100% over 25bp)
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT9040.18589042335310133No Hit
CTCCATGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT8920.18342285136168848No Hit
CTCCATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG7930.16306538243253246No Hit
CTCCATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA7730.1589527624468444No Hit
CTCCATGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA6530.13427704253271588No Hit
CTCCATGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGA6400.13160383954201865No Hit
CTCCATGGGGGAAGACGCGCCTGCGCAGAAGTGACAGCACGAGCGGGATTGG6280.12913626755060578No Hit
CTCCATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC6030.12399549256849568No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGCAG5390.11083510861429383No Hit
CTCCATGGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG5270.10836753662288097No Hit
CTCCATGGGAAGTATTACAGCTCCTGAACTGCCTTCTACTAAATCAGACTTG5250.10795627462431216No Hit
CTCCATGGGGCTCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTG5200.10692811962789014No Hit
CTCCATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACG5110.10507744063433051No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA5070.10425491663719288No Hit
CTCCATGGGGCTCAGCCCGTAGCCCGTCGGTTCCGGAGTAAGTTCCAGGTGG4870.1001422966515048No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGA206.307844E-446.00000432
AACAATT206.307844E-446.00000445
AACCGTA206.307844E-446.00000427
AAGTAAG206.307844E-446.00000416
AATCCAT206.307844E-446.00000424
AATCCAC206.307844E-446.00000432
ACACGGT351.01794285E-746.00000421
ACACGCG206.307844E-446.00000411
AATTTCG206.307844E-446.00000434
TTAATCG206.307844E-446.00000424
ATAGGTG206.307844E-446.00000419
AAATACG206.307844E-446.00000429
TGCCACG206.307844E-446.00000419
CTATCGA206.307844E-446.00000433
CGTGCAT206.307844E-446.00000413
GCGAAAC206.307844E-446.00000441
CTATATC351.01794285E-746.00000442
TAGAATC206.307844E-446.00000445
TTAGAAG206.307844E-446.00000421
GGCGTAA206.307844E-446.00000410
Produced by FastQC (version 0.11.5)