##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527706_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	486308
Sequences flagged as poor quality	0
Sequence length	52
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.161716443077225	33.0	31.0	34.0	31.0	34.0
2	32.36527673819884	34.0	31.0	34.0	30.0	34.0
3	32.7046850966877	34.0	31.0	34.0	31.0	34.0
4	36.30650328598337	37.0	37.0	37.0	35.0	37.0
5	35.863958643493426	37.0	35.0	37.0	35.0	37.0
6	36.111392368622354	37.0	35.0	37.0	35.0	37.0
7	36.377417192396585	37.0	36.0	37.0	35.0	37.0
8	36.51142280201025	37.0	37.0	37.0	35.0	37.0
9	38.55172236525001	39.0	39.0	39.0	37.0	39.0
10	37.7402592595639	39.0	38.0	39.0	35.0	39.0
11	37.54009187593048	39.0	37.0	39.0	35.0	39.0
12	37.26764108342861	39.0	37.0	39.0	34.0	39.0
13	36.97457372693848	39.0	37.0	39.0	33.0	39.0
14	37.89299990952236	40.0	38.0	40.0	33.0	40.0
15	37.99661737006177	40.0	38.0	40.0	33.0	40.0
16	37.76733263692968	40.0	37.0	40.0	33.0	40.0
17	37.92574047722842	40.0	38.0	40.0	33.0	40.0
18	37.96743833126332	40.0	38.0	40.0	34.0	40.0
19	37.448450364789394	40.0	37.0	40.0	32.0	40.0
20	37.72934230981189	40.0	37.0	40.0	33.0	40.0
21	37.87782639808516	40.0	37.0	40.0	33.0	40.0
22	37.78064107520337	40.0	37.0	40.0	33.0	40.0
23	37.758938779538894	40.0	37.0	40.0	33.0	40.0
24	37.76289511996512	40.0	37.0	40.0	33.0	40.0
25	37.63826834022883	40.0	37.0	40.0	33.0	40.0
26	37.26485067076832	40.0	36.0	40.0	32.0	40.0
27	37.191917467942126	40.0	36.0	40.0	32.0	40.0
28	37.09466017421058	40.0	36.0	40.0	31.0	40.0
29	37.04177393750463	40.0	36.0	40.0	31.0	40.0
30	36.67741020094261	39.0	35.0	40.0	30.0	40.0
31	36.63171282397164	39.0	35.0	40.0	30.0	40.0
32	35.945474884229746	39.0	35.0	40.0	29.0	40.0
33	36.01960485947178	39.0	35.0	40.0	29.0	40.0
34	35.91602029989225	39.0	35.0	40.0	28.0	40.0
35	35.99305172853418	39.0	35.0	40.0	29.0	40.0
36	35.76383279732186	38.0	35.0	40.0	28.0	40.0
37	35.25065185026773	38.0	34.0	40.0	25.0	40.0
38	35.03550013571646	38.0	34.0	40.0	25.0	40.0
39	35.01157291263973	38.0	34.0	40.0	25.0	40.0
40	34.87516347664443	38.0	34.0	40.0	25.0	40.0
41	34.42510096482065	38.0	33.0	40.0	23.0	40.0
42	34.47593911677373	38.0	33.0	40.0	24.0	40.0
43	34.31732358916572	37.0	33.0	40.0	23.0	40.0
44	34.27535841483175	37.0	33.0	40.0	23.0	40.0
45	34.05374371797297	37.0	33.0	40.0	23.0	40.0
46	33.78895062388445	36.0	33.0	40.0	23.0	40.0
47	33.565880059550736	36.0	32.0	40.0	22.0	40.0
48	33.581767110555454	36.0	32.0	39.0	23.0	40.0
49	33.48259744853056	35.0	32.0	39.0	23.0	40.0
50	32.992247711326975	35.0	31.0	39.0	20.0	40.0
51	33.34518864587874	35.0	32.0	39.0	21.0	40.0
52	33.04928769421848	35.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	1.0
15	1.0
16	7.0
17	28.0
18	66.0
19	127.0
20	281.0
21	639.0
22	1094.0
23	1893.0
24	3015.0
25	3966.0
26	5508.0
27	7658.0
28	9415.0
29	10963.0
30	12571.0
31	14232.0
32	16423.0
33	20856.0
34	30198.0
35	34605.0
36	48025.0
37	78584.0
38	134660.0
39	51490.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.40367010207522	0.33127153984717506	0.15648519045543152	0.10857316762216537
8	99.35411303124768	0.3946058876267715	0.21755759724289958	0.03372348388264228
9	98.25686601906611	1.0814134252366814	0.3000156279559456	0.36170492774126684
10	56.01984750405094	33.38727719881228	2.90042524490652	7.6924500522302734
11	29.154363078542815	31.284905862128525	18.859241468369838	20.701489590958815
12	34.839443315758736	14.741891969698214	27.136711713564242	23.281953000978802
13	20.58921506534953	14.185043223636049	26.57945170550351	38.646290005510906
14	21.457183513329	24.671607294142806	30.22672873981098	23.64448045271721
15	30.560673482648852	15.519177146993263	25.062306192783172	28.857843177574704
16	27.017651364978573	17.115696225437375	30.518724758794836	25.34792765078921
17	36.884854865640705	19.910632767710997	19.26639084695296	23.938121519695336
18	35.183463977561544	17.53744540496969	22.72901124390304	24.550079373565723
19	29.85350847610979	17.06120401062701	27.572443801047896	25.512843712215304
20	29.69620076165722	26.599192281434814	20.631369420202834	23.073237536705133
21	31.974386602729133	15.730360183258346	29.986962994645367	22.308290219367148
22	28.598748118476358	17.245449385985836	20.59558962632735	33.56021286921046
23	26.173536112916096	28.362272469299292	20.982998428979165	24.481192988805446
24	26.988657394079475	28.53150678171036	20.526908872566356	23.952926951643814
25	26.86939141449452	19.479630193210888	19.667782557556116	33.98319583473848
26	34.360734349424646	19.391825756516447	19.760110876234815	26.487329017824095
27	26.651216924253767	17.634708867631215	20.610395058275824	35.103679149839195
28	27.94669221974551	27.856420211059657	19.693280801467385	24.503606767727447
29	37.13037827878629	19.404986140470648	18.754575289734078	24.71006029100899
30	28.032234715447824	27.164677529466925	19.543170171989768	25.259917583095486
31	36.51019518494452	20.316548360298412	18.937792510096482	24.235463944660584
32	31.0110053710817	18.535783906495475	26.464503976903526	23.988706745519302
33	36.88814496162925	19.179408934255658	19.713227008397972	24.21921909571712
34	28.560089490610892	21.36691150464315	20.75598180576918	29.31701719897678
35	29.61456525494131	27.864234189032466	21.082112570634248	21.439087985391975
36	36.866553706704394	21.71154905944381	18.137065398883014	23.284831834968784
37	30.358332579353004	22.453465704861937	24.22785559768706	22.960346118097995
38	37.43286147873364	20.951331255089368	18.338995040180297	23.276812225996693
39	29.506814611316283	20.66385911808977	19.81316367405019	30.016162596543754
40	27.998716862564464	21.136604785444614	25.08163550671591	25.783042845275013
41	27.153573455505565	20.808828972585275	21.337917533744047	30.699680038165113
42	25.67549783264927	19.86457142387129	28.19036495389753	26.26956578958191
43	26.20170755981806	18.436464133841106	23.294085229936584	32.067743076404255
44	26.424200301043783	19.602391899783676	21.930340442682414	32.04306735649013
45	27.4102009426125	25.6685063786736	22.2060916127228	24.7152010659911
46	27.77437344234518	20.407437261982118	26.44455776997294	25.373631525699764
47	32.550564662724035	20.574820895399622	22.04302623029027	24.831588211586073
48	33.396119331781506	20.911027579229625	22.451615025868378	23.24123806312049
49	32.67702772728394	20.993279978943384	22.462719099829737	23.866973193942933
50	27.863206034036043	23.24308874211405	22.116230866035515	26.77747435781439
51	25.202752165294424	26.46368145290639	22.634215353232932	25.69935102856626
52	31.82283655625653	22.18038773781225	22.107799995064855	23.888975710866365
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	5.0
6	10.0
7	8.5
8	7.0
9	8.5
10	10.0
11	64.0
12	118.0
13	251.5
14	363.5
15	342.0
16	338.0
17	334.0
18	258.0
19	182.0
20	475.0
21	768.0
22	616.5
23	465.0
24	656.0
25	847.0
26	981.0
27	1115.0
28	1273.5
29	1432.0
30	1568.5
31	1705.0
32	2031.5
33	2358.0
34	2429.0
35	2500.0
36	3314.5
37	4129.0
38	4750.5
39	6065.5
40	6759.0
41	6869.0
42	6979.0
43	8500.5
44	10022.0
45	10774.5
46	11527.0
47	12679.5
48	13832.0
49	16218.0
50	18604.0
51	20036.0
52	21468.0
53	25208.5
54	28949.0
55	38063.5
56	47178.0
57	49426.0
58	51674.0
59	50774.0
60	49874.0
61	45514.5
62	41155.0
63	40369.0
64	35315.0
65	31047.0
66	28731.0
67	26415.0
68	22252.0
69	18089.0
70	16569.0
71	15049.0
72	12049.0
73	9049.0
74	9577.5
75	10106.0
76	7119.0
77	4132.0
78	2986.0
79	1840.0
80	1228.5
81	617.0
82	403.0
83	189.0
84	119.5
85	50.0
86	45.0
87	40.0
88	21.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	486308.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.653026477047465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.58331789837625	12.753440206618027
2	9.346175217632895	3.1128420671673096
3	3.442612829536334	1.7198976780147563
4	1.6422794344631724	1.0939569161930298
5	0.8569488176822869	0.7135395675168823
6	0.518614558251528	0.5181901181966984
7	0.35315181823794534	0.411673260567377
8	0.2531332962894363	0.33723483882642274
9	0.2012718404642835	0.30166067595022084
>10	3.482126319688831	15.865459749788199
>50	2.2251034142125086	26.492881054804773
>100	1.0656294375501636	29.75912384743825
>500	0.017287151941717604	1.8537634585489033
>1k	0.011113169105389887	3.468583695929329
>5k	0.001234796567265543	1.59775286443982
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGAAG	7770	1.59775286443982	No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGGAG	3610	0.7423279074166989	No Hit
CTCCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2425	0.49865517326467995	No Hit
CTCCATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	2351	0.4834384793176341	No Hit
CTCCATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2241	0.4608190693963497	No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	1435	0.2950804839731199	No Hit
CTCCATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1423	0.2926129119817071	No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	1263	0.2597119520962024	No Hit
CTCCATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1097	0.22557720621499133	No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC	1023	0.2103605122679454	Illumina Single End Adapter 2 (100% over 25bp)
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	904	0.18589042335310133	No Hit
CTCCATGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	892	0.18342285136168848	No Hit
CTCCATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	793	0.16306538243253246	No Hit
CTCCATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	773	0.1589527624468444	No Hit
CTCCATGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	653	0.13427704253271588	No Hit
CTCCATGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGA	640	0.13160383954201865	No Hit
CTCCATGGGGGAAGACGCGCCTGCGCAGAAGTGACAGCACGAGCGGGATTGG	628	0.12913626755060578	No Hit
CTCCATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	603	0.12399549256849568	No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGCAG	539	0.11083510861429383	No Hit
CTCCATGGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	527	0.10836753662288097	No Hit
CTCCATGGGAAGTATTACAGCTCCTGAACTGCCTTCTACTAAATCAGACTTG	525	0.10795627462431216	No Hit
CTCCATGGGGCTCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTG	520	0.10692811962789014	No Hit
CTCCATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACG	511	0.10507744063433051	No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA	507	0.10425491663719288	No Hit
CTCCATGGGGCTCAGCCCGTAGCCCGTCGGTTCCGGAGTAAGTTCCAGGTGG	487	0.1001422966515048	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.00843087097066057	0.0	0.0	0.0	0.0
8	0.011515335959926631	0.0	0.0	0.0	0.0
9	0.03598542487477072	0.0	0.0	0.0	0.0
10	0.09685220066295434	0.0	0.0	0.0	0.0
11	0.2689653470640006	0.0	0.0	0.0	0.0
12	0.28747213699959695	0.0	0.0	0.0	0.0
13	0.3148210599044227	0.0	0.0	0.0	0.0
14	0.4293575265058358	0.0	0.0	0.0	0.0
15	0.46739926137345056	0.0	0.0	0.0	0.0
16	0.5605501040492856	0.0	0.0	0.0	0.0
17	0.6181267838489188	0.0	0.0	0.0	0.0
18	0.6516446367322767	0.0	0.0	0.0	0.0
19	0.689480740600607	0.0	0.0	0.0	0.0
20	0.7297844164603502	0.0	0.0	0.0	0.0
21	0.7532263503787723	0.0	0.0	0.0	0.0
22	0.7760513912993412	0.0	0.0	0.0	0.0
23	0.8114199231762587	0.0	0.0	0.0	0.0
24	0.8630333039966441	0.0	0.0	0.0	0.0
25	0.8881202859093414	0.0	0.0	0.0	0.0
26	1.0121157784778372	0.0	0.0	0.0	0.0
27	1.0692811962789015	0.0	0.0	0.0	0.0
28	1.3553139162835075	0.0	0.0	0.0	0.0
29	1.396234485141104	0.0	0.0	0.0	0.0
30	1.5144723097296364	0.0	0.0	0.0	0.0
31	1.5434662806287374	0.0	0.0	0.0	0.0
32	1.610501986395453	0.0	0.0	0.0	0.0
33	1.6520394482509027	0.0	0.0	0.0	0.0
34	1.6941938031042056	0.0	0.0	0.0	0.0
35	1.882757429448004	0.0	0.0	0.0	0.0
36	1.9210047953149032	0.0	0.0	0.0	0.0
37	1.9740575931302795	0.0	0.0	0.0	0.0
38	2.0151837929871603	0.0	0.0	0.0	0.0
39	2.0435608708884083	0.0	0.0	0.0	0.0
40	2.0717323177903717	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGA	20	6.307844E-4	46.000004	32
AACAATT	20	6.307844E-4	46.000004	45
AACCGTA	20	6.307844E-4	46.000004	27
AAGTAAG	20	6.307844E-4	46.000004	16
AATCCAT	20	6.307844E-4	46.000004	24
AATCCAC	20	6.307844E-4	46.000004	32
ACACGGT	35	1.01794285E-7	46.000004	21
ACACGCG	20	6.307844E-4	46.000004	11
AATTTCG	20	6.307844E-4	46.000004	34
TTAATCG	20	6.307844E-4	46.000004	24
ATAGGTG	20	6.307844E-4	46.000004	19
AAATACG	20	6.307844E-4	46.000004	29
TGCCACG	20	6.307844E-4	46.000004	19
CTATCGA	20	6.307844E-4	46.000004	33
CGTGCAT	20	6.307844E-4	46.000004	13
GCGAAAC	20	6.307844E-4	46.000004	41
CTATATC	35	1.01794285E-7	46.000004	42
TAGAATC	20	6.307844E-4	46.000004	45
TTAGAAG	20	6.307844E-4	46.000004	21
GGCGTAA	20	6.307844E-4	46.000004	10
>>END_MODULE