FastQCFastQC Report
Fri 17 Jun 2016
SRR1527705_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527705_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4629956
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC615481.329343086629765No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG378020.8164656424380707No Hit
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC240230.5188602224297596No Hit
TTAACTGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGA181500.39201236469633843No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT180310.38944214588648357No Hit
TTAACTGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGAC154230.3331133168436158No Hit
TTAACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT142050.30680637137804334No Hit
TTAACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT129860.2804778274350771No Hit
TTAACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC121520.2624646972886999No Hit
TTAACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG108230.2337603208324226No Hit
TTAACTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG96410.2082309205530247No Hit
TTAACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA89740.1938247361314017No Hit
TTAACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC85280.18419181521379469No Hit
TTAACTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT83490.18032568776031566No Hit
TTAACTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG82220.17758268113131095No Hit
TTAACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC80060.17291741001426364No Hit
TTAACTGGGGCTTCCCAGCCCAGAGCTCGCCCAGACTTCCACACGCGCAC71780.15503387073224886No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC69470.15004462245429548No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTG57580.1243640328331414No Hit
TTAACTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG56580.12220418509376763No Hit
TTAACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA55170.11915879978125063No Hit
TTAACTGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTA51970.11224728701525458No Hit
TTAACTGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGC50700.1095042803862499No Hit
TTAACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT50160.10833796260698805No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAA207.8589533E-444.00000420
TAACTGG4762150.042.2310942
TTAACTG4800950.042.213771
AACTGGG4654250.042.1967053
ACTGGGG2636400.042.0281454
ACTGGGA1255450.042.0093154
ACTGGGC507300.041.8229874
ACTGGGT278550.041.7648544
CTGGGGG1085250.041.646445
CTGGGAT354600.041.536945
TGGGGGG548400.041.28416
CTGGGGA678650.041.1829385
CTGGGGC542800.040.6764955
CTGGGAC246300.040.4360545
TGGGGAG311250.040.4305236
TGGGATA50150.040.3589256
TGGGATT123600.040.2977336
TAACTAG20600.040.2621352
CTGGGGT388150.040.1344875
TGGGGCT289800.040.113186
Produced by FastQC (version 0.11.5)