##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527705_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4629956
Sequences flagged as poor quality	0
Sequence length	50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.632509682597416	33.0	31.0	34.0	31.0	34.0
2	33.073865280793164	34.0	33.0	34.0	31.0	34.0
3	33.13001613838231	34.0	33.0	34.0	31.0	34.0
4	36.63398226678612	37.0	37.0	37.0	35.0	37.0
5	36.522637364156374	37.0	37.0	37.0	35.0	37.0
6	36.56466692124072	37.0	37.0	37.0	35.0	37.0
7	36.572734600501605	37.0	37.0	37.0	35.0	37.0
8	36.69859044016833	37.0	37.0	37.0	35.0	37.0
9	38.76512714159703	39.0	39.0	39.0	39.0	39.0
10	38.3251585976195	39.0	39.0	39.0	37.0	39.0
11	38.13736977198055	39.0	39.0	39.0	37.0	39.0
12	37.63565096514956	39.0	37.0	39.0	35.0	39.0
13	37.52401556299887	39.0	37.0	39.0	35.0	39.0
14	38.24377251101306	40.0	38.0	40.0	35.0	40.0
15	38.29940997279456	40.0	38.0	40.0	35.0	40.0
16	38.34094557270091	40.0	38.0	40.0	35.0	40.0
17	38.212677615078846	40.0	38.0	40.0	34.0	40.0
18	38.14015705548822	40.0	38.0	40.0	34.0	40.0
19	38.13950888518163	40.0	38.0	40.0	34.0	40.0
20	38.041904501900234	40.0	38.0	40.0	34.0	40.0
21	38.058981554036365	40.0	38.0	40.0	34.0	40.0
22	38.10755760961875	40.0	38.0	40.0	34.0	40.0
23	38.07937958805656	40.0	38.0	40.0	34.0	40.0
24	38.03073506530084	40.0	38.0	40.0	34.0	40.0
25	38.02212979993762	40.0	37.0	40.0	34.0	40.0
26	37.924649823886014	40.0	37.0	40.0	34.0	40.0
27	37.81338850736378	40.0	37.0	40.0	34.0	40.0
28	37.635388759634	40.0	36.0	40.0	34.0	40.0
29	37.65989806382609	40.0	37.0	40.0	34.0	40.0
30	37.76415132238838	40.0	37.0	40.0	34.0	40.0
31	37.77933159623979	40.0	37.0	40.0	34.0	40.0
32	37.780549102410475	40.0	36.0	40.0	34.0	40.0
33	37.684737608737535	40.0	36.0	40.0	34.0	40.0
34	37.31339909061771	40.0	35.0	40.0	33.0	40.0
35	37.28552755144973	40.0	35.0	40.0	33.0	40.0
36	37.26069232623377	40.0	35.0	40.0	33.0	40.0
37	37.18359332140521	40.0	35.0	40.0	33.0	40.0
38	37.09888085329536	40.0	35.0	40.0	33.0	40.0
39	36.95586869508047	40.0	35.0	40.0	33.0	40.0
40	36.75656852894498	40.0	35.0	40.0	32.0	40.0
41	36.588018331059736	39.0	35.0	40.0	32.0	40.0
42	36.50397131203839	39.0	35.0	40.0	32.0	40.0
43	36.38225806033578	39.0	35.0	40.0	31.0	40.0
44	36.07986231402631	39.0	35.0	40.0	31.0	40.0
45	36.035532303114756	39.0	35.0	40.0	30.0	40.0
46	35.882171234456656	38.0	35.0	40.0	30.0	40.0
47	35.76759800741087	38.0	35.0	40.0	30.0	40.0
48	35.68782014343117	38.0	35.0	40.0	30.0	40.0
49	35.59592099795333	37.0	35.0	40.0	30.0	40.0
50	35.098313245309456	37.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	3.0
12	8.0
13	4.0
14	12.0
15	42.0
16	78.0
17	254.0
18	522.0
19	988.0
20	1713.0
21	2874.0
22	4429.0
23	6685.0
24	9715.0
25	13666.0
26	18474.0
27	24484.0
28	32799.0
29	44800.0
30	62844.0
31	84771.0
32	103660.0
33	129139.0
34	280418.0
35	371898.0
36	317559.0
37	607733.0
38	1408524.0
39	1101856.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.4974682264799	0.39581369671763617	0.0903464309380046	0.01637164586445314
8	99.24472284401838	0.644044997403863	0.07105899062539688	0.04017316795235203
9	97.59135939952776	1.2732518408382283	0.45073862472991105	0.6846501349040899
10	56.181181851404205	26.640425956531764	6.10742305110459	11.070969140959438
11	37.904312697572074	23.185835891313005	19.456534792123296	19.45331661899163
12	32.51901313964971	16.275943011121488	25.881217877664497	25.323825971564307
13	26.06441616291818	15.655440354076799	29.028763988253885	29.25137949475114
14	24.99606907711434	16.674694100764672	31.792764337285277	26.53647248483571
15	26.294850318231966	18.1438441315641	29.293604518055893	26.26770103214804
16	31.452177947263433	18.86566524606281	25.932341473655473	23.749815333018283
17	31.437339793293933	19.330723661304773	23.885907339076223	25.34602920632507
18	30.8469886106909	19.962349534207235	24.1970766028878	24.99358525221406
19	29.551598330524094	21.58109061943569	23.38896957120111	25.478341478839106
20	30.20834323263547	22.0280495106217	23.860183552500285	23.903423704242545
21	30.048795280127933	21.95256283213059	25.142787534049997	22.85585435369148
22	28.67316665644339	20.37630595193561	26.926713774385764	24.02381361723524
23	30.50823809124752	20.268270368012136	25.21671912216876	24.006772418571583
24	31.43403522625269	20.49354248722882	22.591856164507824	25.48056612201066
25	28.801569604549158	20.41241860613794	22.91691324928358	27.869098540029324
26	26.681074290986786	20.069650769899326	25.015010941788645	28.234263997325243
27	25.920807886727214	19.17387551847145	25.10321912346467	29.80209747133666
28	27.243088271249228	22.119670251725935	23.02764864288127	27.609592834143566
29	30.19994142492931	21.265299281461854	22.522892226189622	26.011867067419214
30	29.39382145316284	19.936841732405234	25.42039276399171	25.24894405044022
31	31.829071377784153	19.75556571163959	24.247833024763086	24.16752988581317
32	33.87762216314798	19.708826606559544	22.689394888417947	23.724156341874522
33	33.59574043468231	19.597659243413975	23.440481939785172	23.366118382118533
34	30.392513449371872	21.45962078257331	27.252354018051143	20.895511750003674
35	28.951592628526058	25.308987817594815	25.358081156710778	20.381338397168353
36	28.795846008039817	25.888647753887945	22.6947297123342	22.620776525738044
37	30.42467358221115	25.26596365062648	21.370311078550206	22.93905168861216
38	30.282663593347326	24.727232828994484	21.97444208973044	23.015661487927748
39	30.663271961979767	22.53831353904875	21.85063961730954	24.947774881661942
40	29.120881494338175	22.450882902558902	21.564459791842513	26.86377581126041
41	26.206426151782004	23.881350060346147	22.629826287766015	27.28239750010583
42	24.783496862605173	21.919171586079866	23.94223616811909	29.355095383195867
43	24.59816032808951	20.86967997104076	24.755246054174165	29.77691364669556
44	26.8862382277499	19.5493002525294	24.07975367368502	29.484707846035686
45	28.829496435819262	20.70786417840688	23.037907919643295	27.424731466130563
46	27.419807013284792	24.12482969600575	22.500969771634978	25.954393519074483
47	26.896929474059796	22.52282743075744	24.069947964948263	26.5102951302345
48	27.499116622274595	21.41348643486029	25.668472875336178	25.418924067528938
49	29.29939290999742	21.44065731942161	23.64508431613605	25.614865454444924
50	25.869122730324	22.078935523361345	23.8456477772143	28.206293969100354
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	18.5
2	37.0
3	30.0
4	23.0
5	176.0
6	329.0
7	1420.0
8	2511.0
9	5772.5
10	9034.0
11	13561.0
12	18088.0
13	15972.0
14	13856.0
15	14401.5
16	14947.0
17	15673.0
18	16399.0
19	16373.0
20	16347.0
21	17640.0
22	18933.0
23	21962.0
24	24991.0
25	27772.5
26	30554.0
27	35453.5
28	40353.0
29	43728.5
30	47104.0
31	53904.0
32	60704.0
33	63215.0
34	65726.0
35	76692.5
36	87659.0
37	90933.0
38	94207.0
39	103885.0
40	113563.0
41	119860.0
42	126157.0
43	137452.0
44	148747.0
45	166315.5
46	183884.0
47	196168.5
48	208453.0
49	215200.0
50	221947.0
51	243873.0
52	265799.0
53	305370.5
54	344942.0
55	393288.0
56	441634.0
57	461427.0
58	481220.0
59	485924.5
60	490629.0
61	418883.0
62	347137.0
63	289710.5
64	232284.0
65	190230.0
66	148176.0
67	134034.5
68	119893.0
69	98709.5
70	77526.0
71	71898.5
72	66271.0
73	49213.0
74	32155.0
75	20710.0
76	9265.0
77	7509.5
78	5754.0
79	3801.0
80	1848.0
81	1194.5
82	541.0
83	408.0
84	275.0
85	158.5
86	42.0
87	24.5
88	7.0
89	6.0
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4629956.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.278288886597313
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.97649017751235	4.5836108857589295
2	10.604152243793047	1.5436016685556977
3	4.214881771847105	0.9203138147506914
4	2.0825033204543706	0.6062824309426018
5	1.109190243065275	0.4036503509612082
6	0.794528144093888	0.34696832167484
7	0.5893369838333558	0.30025553739265654
8	0.459438820077032	0.26751427665904354
9	0.37867079641391366	0.24804679042965072
>10	9.771788166981352	20.253430101580413
>50	4.496439618178126	22.626924076349674
>100	2.375457014216249	29.884047674418174
>500	0.09323825402554016	4.657105245962105
>1k	0.04661912701277008	6.237110328486534
>5k	0.004238102455706371	2.160573390378436
>10k+	0.0030272160397902654	4.960565105699231
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	61548	1.329343086629765	No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	37802	0.8164656424380707	No Hit
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	24023	0.5188602224297596	No Hit
TTAACTGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGA	18150	0.39201236469633843	No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	18031	0.38944214588648357	No Hit
TTAACTGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGAC	15423	0.3331133168436158	No Hit
TTAACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	14205	0.30680637137804334	No Hit
TTAACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	12986	0.2804778274350771	No Hit
TTAACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	12152	0.2624646972886999	No Hit
TTAACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	10823	0.2337603208324226	No Hit
TTAACTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	9641	0.2082309205530247	No Hit
TTAACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	8974	0.1938247361314017	No Hit
TTAACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	8528	0.18419181521379469	No Hit
TTAACTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	8349	0.18032568776031566	No Hit
TTAACTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	8222	0.17758268113131095	No Hit
TTAACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	8006	0.17291741001426364	No Hit
TTAACTGGGGCTTCCCAGCCCAGAGCTCGCCCAGACTTCCACACGCGCAC	7178	0.15503387073224886	No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	6947	0.15004462245429548	No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTG	5758	0.1243640328331414	No Hit
TTAACTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	5658	0.12220418509376763	No Hit
TTAACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	5517	0.11915879978125063	No Hit
TTAACTGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTA	5197	0.11224728701525458	No Hit
TTAACTGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGC	5070	0.1095042803862499	No Hit
TTAACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	5016	0.10833796260698805	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.019330637267395197	0.0	0.0	0.0	0.0
8	0.02453587031928597	0.0	0.0	0.0	0.0
9	0.027386869335259342	0.0	0.0	0.0	0.0
10	0.042462606556088224	0.0	0.0	0.0	0.0
11	0.05803510875697307	0.0	0.0	0.0	0.0
12	0.07056222564534091	0.0	0.0	0.0	0.0
13	0.07840247293926768	0.0	0.0	0.0	0.0
14	0.08619952327840696	0.0	0.0	0.0	0.0
15	0.09596203506037639	0.0	0.0	0.0	0.0
16	0.10874833367746907	0.0	0.0	0.0	0.0
17	0.12067069319881225	0.0	0.0	0.0	0.0
18	0.1327442420619116	0.0	0.0	0.0	0.0
19	0.14548734372421682	0.0	0.0	0.0	0.0
20	0.15753929410992243	0.0	0.0	0.0	0.0
21	0.16827373737461004	0.0	0.0	0.0	0.0
22	0.17907297607147887	0.0	0.0	0.0	0.0
23	0.19116812341197195	0.0	0.0	0.0	0.0
24	0.20298249054634646	0.0	0.0	0.0	0.0
25	0.2144296835650274	0.0	0.0	0.0	0.0
26	0.22840389843877565	0.0	0.0	0.0	0.0
27	0.2400022807992128	0.0	0.0	0.0	0.0
28	0.25518601040701033	0.0	0.0	0.0	0.0
29	0.2664388171291477	0.0	0.0	0.0	0.0
30	0.2792467142236341	0.0	0.0	0.0	0.0
31	0.2892900062117221	0.0	0.0	0.0	0.0
32	0.30032682815992207	0.0	0.0	0.0	0.0
33	0.31082368817327855	0.0	0.0	0.0	0.0
34	0.3220332979406284	0.0	0.0	0.0	0.0
35	0.33853453466944394	0.0	0.0	0.0	0.0
36	0.353761461232029	0.0	0.0	0.0	0.0
37	0.3666125552813029	0.0	0.0	0.0	0.0
38	0.3789884828279146	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTAA	20	7.8589533E-4	44.000004	20
TAACTGG	476215	0.0	42.231094	2
TTAACTG	480095	0.0	42.21377	1
AACTGGG	465425	0.0	42.196705	3
ACTGGGG	263640	0.0	42.028145	4
ACTGGGA	125545	0.0	42.009315	4
ACTGGGC	50730	0.0	41.822987	4
ACTGGGT	27855	0.0	41.764854	4
CTGGGGG	108525	0.0	41.64644	5
CTGGGAT	35460	0.0	41.53694	5
TGGGGGG	54840	0.0	41.2841	6
CTGGGGA	67865	0.0	41.182938	5
CTGGGGC	54280	0.0	40.676495	5
CTGGGAC	24630	0.0	40.436054	5
TGGGGAG	31125	0.0	40.430523	6
TGGGATA	5015	0.0	40.358925	6
TGGGATT	12360	0.0	40.297733	6
TAACTAG	2060	0.0	40.262135	2
CTGGGGT	38815	0.0	40.134487	5
TGGGGCT	28980	0.0	40.11318	6
>>END_MODULE