FastQCFastQC Report
Fri 17 Jun 2016
SRR1527704_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527704_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6948912
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC902091.2981744480286985No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT474850.6833443854232145No Hit
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA359450.5172752223657459No Hit
TTAACTGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC272660.39237797226385945No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC270760.3896437312776446No Hit
TTAACTGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCT227600.32753328866447007No Hit
TTAACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT206970.2978451878509902No Hit
TTAACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT175260.2522121448652681No Hit
TTAACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC165280.23785018431662394No Hit
TTAACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC161460.2323529208601289No Hit
TTAACTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG145160.2088960113468123No Hit
TTAACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC134280.19323888401522427No Hit
TTAACTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC129790.18677744084253764No Hit
TTAACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA127590.18361147759534155No Hit
TTAACTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG125940.18123700515994445No Hit
TTAACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC117440.16900487443214132No Hit
TTAACTGGGGCTTCCCAGCCCAGAGCTCGCCCAGACTTCCACACGCGCACGG109940.15821181790760913No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT96590.13900017729394185No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC95550.13750354012254004No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCT85330.12279620176511086No Hit
TTAACTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC84890.12216300911567163No Hit
TTAACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG81700.11757236240723729No Hit
TTAACTGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT80230.11545692332842897No Hit
TTAACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG77130.11099579329828899No Hit
TTAACTGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT74770.10759957817856954No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT70110.10089349239132687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTGG7160150.044.123422
TTAACTG7213800.044.1038971
AACTGGG7001450.044.0729983
ACTGGGA1874900.043.913334
ACTGGGG3986950.043.8551524
ACTGGGC758400.043.777034
ACTGGGT418800.043.440784
CTGGGGG1625050.043.4141735
TGGGGGG812650.043.0905086
CTGGGAT527150.042.8236775
CTGGGGA1032950.042.7958765
CTGGGAC365100.042.41555
CTGGGGC835550.042.4050035
TAACTAG29250.042.225642
TGGGATA70300.041.8449486
TGGGGGC384000.041.7833336
TGGGGAG473200.041.7810636
CTGGGGT591800.041.6704985
TGGGGCT453600.041.553136
TGGGGTC174250.041.274616
Produced by FastQC (version 0.11.5)