##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527704_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6948912
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27299971563894	33.0	31.0	34.0	31.0	34.0
2	32.8180971352062	34.0	31.0	34.0	31.0	34.0
3	32.78984594998469	34.0	31.0	34.0	31.0	34.0
4	36.50663096611383	37.0	37.0	37.0	35.0	37.0
5	36.31314657603953	37.0	37.0	37.0	35.0	37.0
6	36.38774127518092	37.0	37.0	37.0	35.0	37.0
7	36.433213573578136	37.0	36.0	37.0	35.0	37.0
8	36.59567728012673	37.0	37.0	37.0	35.0	37.0
9	38.685549910547145	39.0	39.0	39.0	38.0	39.0
10	38.09292764104654	39.0	38.0	39.0	37.0	39.0
11	37.803880089429825	39.0	38.0	39.0	35.0	39.0
12	37.293279581033694	39.0	37.0	39.0	35.0	39.0
13	37.17392938635574	39.0	37.0	39.0	34.0	39.0
14	37.90310814124571	40.0	38.0	40.0	34.0	40.0
15	37.97164664051005	40.0	38.0	40.0	34.0	40.0
16	38.01640674108407	40.0	38.0	40.0	34.0	40.0
17	37.889741156601204	40.0	37.0	40.0	33.0	40.0
18	37.881759475440184	40.0	37.0	40.0	34.0	40.0
19	37.8911102917982	40.0	37.0	40.0	34.0	40.0
20	37.85927019942115	40.0	37.0	40.0	33.0	40.0
21	37.85489095271317	40.0	37.0	40.0	34.0	40.0
22	37.859662059326695	40.0	37.0	40.0	34.0	40.0
23	37.85064223003543	40.0	37.0	40.0	34.0	40.0
24	37.805464222312786	40.0	37.0	40.0	34.0	40.0
25	37.78991516945386	40.0	37.0	40.0	34.0	40.0
26	37.67043099696758	40.0	36.0	40.0	34.0	40.0
27	37.56218815262015	40.0	36.0	40.0	34.0	40.0
28	37.418684968236754	40.0	36.0	40.0	33.0	40.0
29	37.35583052426049	40.0	35.0	40.0	33.0	40.0
30	37.21359199828692	40.0	35.0	40.0	33.0	40.0
31	37.11203048765044	40.0	35.0	40.0	32.0	40.0
32	37.053342451307486	39.0	35.0	40.0	32.0	40.0
33	36.9234632702213	39.0	35.0	40.0	32.0	40.0
34	36.6219565019675	39.0	35.0	40.0	31.0	40.0
35	36.540851862852776	39.0	35.0	40.0	31.0	40.0
36	36.560059330151255	39.0	35.0	40.0	31.0	40.0
37	36.50242613519929	39.0	35.0	40.0	31.0	40.0
38	36.42094647334719	39.0	35.0	40.0	31.0	40.0
39	36.28987674617264	38.0	35.0	40.0	31.0	40.0
40	36.062818754936025	38.0	35.0	40.0	30.0	40.0
41	35.90014609481312	38.0	35.0	40.0	30.0	40.0
42	35.81673577100991	38.0	35.0	40.0	30.0	40.0
43	35.65653903805373	38.0	35.0	40.0	30.0	40.0
44	35.508870453388965	38.0	34.0	40.0	29.0	40.0
45	35.375913956026494	37.0	34.0	40.0	29.0	40.0
46	35.21001316465081	37.0	34.0	40.0	28.0	40.0
47	35.01646617484867	37.0	34.0	40.0	28.0	40.0
48	34.83468188976922	36.0	34.0	40.0	27.0	40.0
49	35.11734283007181	37.0	34.0	40.0	28.0	40.0
50	35.199684209556835	36.0	34.0	40.0	29.0	40.0
51	35.173908375872365	36.0	34.0	40.0	29.0	40.0
52	34.71929533716933	35.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	5.0
13	6.0
14	22.0
15	34.0
16	113.0
17	361.0
18	904.0
19	1893.0
20	3195.0
21	5752.0
22	9313.0
23	13955.0
24	20805.0
25	28946.0
26	39427.0
27	53454.0
28	71059.0
29	93852.0
30	121501.0
31	152485.0
32	196218.0
33	268374.0
34	527270.0
35	549133.0
36	620056.0
37	1031564.0
38	2064355.0
39	1074857.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.55594487309668	0.37512347256664064	0.058224942264342966	0.010706712072335929
8	99.26218665598297	0.6434676392505762	0.05900204233410928	0.03534366243233473
9	97.57946279935622	1.2791354963194237	0.4394788709369179	0.7019228333874425
10	56.112539632103555	26.706482971722767	6.102638801585054	11.07833859458862
11	37.87987241743743	23.216641684338498	19.38684789791553	19.516638000308536
12	32.4874167351666	16.28583006951304	25.87494272484671	25.35181047047365
13	25.977045039568786	15.667920388112556	29.098569675367887	29.256464896950774
14	25.000359768550815	16.64798748350821	31.788458394637892	26.563194353303075
15	26.38141049994589	18.09042912041482	29.27846834151879	26.249692038120497
16	31.455269544354568	18.829192253406003	25.908473153782925	23.807065048456508
17	31.370220258941256	19.30761247228343	23.927875327821106	25.39429194095421
18	30.87398430142733	19.91270000253277	24.285902598852886	24.927413097187014
19	29.594690506945547	21.57770885571727	23.329033379613957	25.498567257723227
20	30.20690145450108	21.92166198104106	23.93689832307561	23.934538241382246
21	30.11199451079536	21.8693228522681	25.146713615023476	22.87196902191307
22	28.664875882728115	20.355128975586396	26.92297729486285	24.05701784682264
23	30.60582721438982	20.203752760144322	25.14703021134819	24.043389814117663
24	31.409377468012256	20.42698195055571	22.658165767533102	25.505474813898925
25	28.770662227410565	20.3588273962888	22.95395307927342	27.916557297027218
26	26.700741065651716	19.970392487341904	25.062009707418948	28.266856739587435
27	25.954638654223857	19.09473022539356	25.080386684994714	29.87024443538787
28	27.238436750961874	22.123880688084697	22.99450619032159	27.643176370631835
29	30.20701658043734	21.148706444980164	22.56786386127785	26.076413113304643
30	29.464929186036603	19.836429069759408	25.417173220786214	25.281468523417765
31	31.90580050517261	19.656890172159326	24.196636826023987	24.240672496644077
32	33.89442836518868	19.676260110935353	22.70069041023976	23.728621113636205
33	33.599072775709345	19.489151683026062	23.448721181100005	23.463054360164584
34	30.290900215746003	21.345586186729665	27.338250937700753	21.025262659823582
35	28.992970410331864	25.232439265312323	25.34972381287891	20.424866511476907
36	28.800479844902338	25.874582956295892	22.71603094124663	22.60890625755514
37	30.49497532851186	25.193828904438565	21.36803574430069	22.943160022748884
38	30.302700624212825	24.620113191820533	22.018770708277785	23.058415475688857
39	30.740812374656635	22.41101916386335	21.821790231334056	25.02637823014596
40	29.07070919879256	22.385159000430573	21.62478097290626	26.91935082787061
41	26.230710649379358	23.786342379929405	22.66360546802147	27.31934150266977
42	24.86128763754671	21.76093178327773	23.970270453849466	29.40751012532609
43	24.662364410428566	20.766992012562543	24.719668345202816	29.85097523180607
44	26.730803325758046	19.447533657067467	24.1825195080899	29.639143509084587
45	28.89869665927558	20.58975563368769	23.05536463837792	27.456183068658806
46	27.351332122208483	23.99450446343255	22.558308408568134	26.095855005790835
47	26.918285912960187	22.38393578735779	24.115832809510323	26.581945490171698
48	27.598449944394172	21.246390801898198	25.729927217383096	25.425232036324534
49	29.281950901090703	21.36033669731319	23.712417138107377	25.645295263488734
50	25.889722016914302	21.976505098927717	23.884199425751827	28.249573458406154
51	24.827239141897323	22.016957474781666	25.319675943514607	27.8361274398064
52	24.973938366178764	21.95504850255695	25.468807203199578	27.60220592806471
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	31.0
2	62.0
3	47.5
4	33.0
5	231.0
6	429.0
7	1934.5
8	3440.0
9	8044.0
10	12648.0
11	18622.5
12	24597.0
13	21688.0
14	19523.5
15	20268.0
16	21258.5
17	22249.0
18	21899.0
19	21549.0
20	23079.5
21	24610.0
22	27967.5
23	31325.0
24	36274.0
25	41223.0
26	44731.5
27	48240.0
28	55103.5
29	61967.0
30	66932.0
31	71897.0
32	79552.0
33	87207.0
34	99734.0
35	112261.0
36	124283.0
37	136305.0
38	137423.0
39	149133.5
40	159726.0
41	176139.5
42	192553.0
43	206419.5
44	220286.0
45	241451.5
46	262617.0
47	277411.0
48	292205.0
49	307975.0
50	323745.0
51	356075.5
52	388406.0
53	451165.0
54	513924.0
55	554940.0
56	595956.0
57	647658.5
58	699361.0
59	707606.5
60	715852.0
61	617577.5
62	519303.0
63	453576.0
64	323000.5
65	258152.0
66	223135.0
67	188118.0
68	164462.0
69	140806.0
70	125755.5
71	110705.0
72	86698.5
73	62692.0
74	43096.5
75	23501.0
76	16378.5
77	9256.0
78	6972.0
79	4688.0
80	2902.0
81	1116.0
82	699.0
83	282.0
84	204.0
85	126.0
86	80.5
87	35.0
88	25.0
89	11.0
90	7.0
91	3.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	6948912.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.755227072128344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.57729425443176	5.395877160074091
2	9.709138055652094	1.5059314059244937
3	4.191416950223269	0.9751617060884737
4	2.226769395267117	0.6907640919024963
5	1.20812490104939	0.4684641469565303
6	0.7635400996783427	0.3552856111008635
7	0.521660034477831	0.28319144152809167
8	0.3771776559705907	0.2340078694868029
9	0.2772984435202515	0.19354611566225766
>10	4.622458501164604	9.958217137341084
>50	3.621299586592501	20.286770466807248
>100	2.7308022787526762	38.22914889318405
>500	0.1133706039084088	5.866747358265884
>1k	0.05298038025785118	7.798692533584539
>5k	0.0035196756115355677	1.8586629110129667
>10k+	0.0031491834419002445	5.8995311510801525
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	90209	1.2981744480286985	No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	47485	0.6833443854232145	No Hit
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	35945	0.5172752223657459	No Hit
TTAACTGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC	27266	0.39237797226385945	No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	27076	0.3896437312776446	No Hit
TTAACTGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCT	22760	0.32753328866447007	No Hit
TTAACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	20697	0.2978451878509902	No Hit
TTAACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	17526	0.2522121448652681	No Hit
TTAACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	16528	0.23785018431662394	No Hit
TTAACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	16146	0.2323529208601289	No Hit
TTAACTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	14516	0.2088960113468123	No Hit
TTAACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	13428	0.19323888401522427	No Hit
TTAACTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	12979	0.18677744084253764	No Hit
TTAACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	12759	0.18361147759534155	No Hit
TTAACTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	12594	0.18123700515994445	No Hit
TTAACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	11744	0.16900487443214132	No Hit
TTAACTGGGGCTTCCCAGCCCAGAGCTCGCCCAGACTTCCACACGCGCACGG	10994	0.15821181790760913	No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	9659	0.13900017729394185	No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	9555	0.13750354012254004	No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCT	8533	0.12279620176511086	No Hit
TTAACTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	8489	0.12216300911567163	No Hit
TTAACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	8170	0.11757236240723729	No Hit
TTAACTGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT	8023	0.11545692332842897	No Hit
TTAACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	7713	0.11099579329828899	No Hit
TTAACTGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT	7477	0.10759957817856954	No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	7011	0.10089349239132687	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.017643049732101946	0.0	0.0	0.0	0.0
8	0.022118570504274626	0.0	0.0	0.0	0.0
9	0.02468012258609693	0.0	0.0	0.0	0.0
10	0.03777569783586265	0.0	0.0	0.0	0.0
11	0.05202253244824514	0.0	0.0	0.0	0.0
12	0.06366464275270718	0.0	0.0	0.0	0.0
13	0.07068732486466946	0.0	0.0	0.0	0.0
14	0.07766683475053361	0.0	0.0	0.0	0.0
15	0.08702081707179483	0.0	0.0	0.0	0.0
16	0.09879244405455127	0.0	0.0	0.0	0.0
17	0.10977258022550869	0.0	0.0	0.0	0.0
18	0.12034977561955022	0.0	0.0	0.0	0.0
19	0.1324811711531244	0.0	0.0	0.0	0.0
20	0.14363399622847434	0.0	0.0	0.0	0.0
21	0.15372190639340375	0.0	0.0	0.0	0.0
22	0.16409763139898736	0.0	0.0	0.0	0.0
23	0.17517850276417374	0.0	0.0	0.0	0.0
24	0.18571252593211715	0.0	0.0	0.0	0.0
25	0.19610264167973346	0.0	0.0	0.0	0.0
26	0.20816208350314408	0.0	0.0	0.0	0.0
27	0.21846585479856415	0.0	0.0	0.0	0.0
28	0.2329573320255027	0.0	0.0	0.0	0.0
29	0.2440525941327218	0.0	0.0	0.0	0.0
30	0.2550327303036792	0.0	0.0	0.0	0.0
31	0.2648184348859217	0.0	0.0	0.0	0.0
32	0.2751365969233745	0.0	0.0	0.0	0.0
33	0.28441862553447217	0.0	0.0	0.0	0.0
34	0.2951685098329062	0.0	0.0	0.0	0.0
35	0.30991902041643354	0.0	0.0	0.0	0.0
36	0.3241514642867833	0.0	0.0	0.0	0.0
37	0.33730460250467986	0.0	0.0	0.0	0.0
38	0.34827034793360456	0.0	0.0	0.0	0.0
39	0.35858850997105735	0.0	0.0	0.0	0.0
40	0.36926644055932784	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACTGG	716015	0.0	44.12342	2
TTAACTG	721380	0.0	44.103897	1
AACTGGG	700145	0.0	44.072998	3
ACTGGGA	187490	0.0	43.91333	4
ACTGGGG	398695	0.0	43.855152	4
ACTGGGC	75840	0.0	43.77703	4
ACTGGGT	41880	0.0	43.44078	4
CTGGGGG	162505	0.0	43.414173	5
TGGGGGG	81265	0.0	43.090508	6
CTGGGAT	52715	0.0	42.823677	5
CTGGGGA	103295	0.0	42.795876	5
CTGGGAC	36510	0.0	42.4155	5
CTGGGGC	83555	0.0	42.405003	5
TAACTAG	2925	0.0	42.22564	2
TGGGATA	7030	0.0	41.844948	6
TGGGGGC	38400	0.0	41.783333	6
TGGGGAG	47320	0.0	41.781063	6
CTGGGGT	59180	0.0	41.670498	5
TGGGGCT	45360	0.0	41.55313	6
TGGGGTC	17425	0.0	41.27461	6
>>END_MODULE