FastQCFastQC Report
Fri 17 Jun 2016
SRR1527703_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527703_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6992729
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC852891.2196811859861867No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT457420.6541366038924145No Hit
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA341770.4887505292997913No Hit
TTAACTGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC258720.36998430798619536No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC255820.3658371431239506No Hit
TTAACTGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCT219940.31452670338003946No Hit
TTAACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT197570.28253633166679276No Hit
TTAACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT171820.2457123678037573No Hit
TTAACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC155860.22288866049292058No Hit
TTAACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC154240.2205719683974597No Hit
TTAACTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG137280.19631820423757304No Hit
TTAACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC131670.188295585314403No Hit
TTAACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA124220.1776416617889811No Hit
TTAACTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG120350.1721073417831579No Hit
TTAACTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC119870.17142091449561395No Hit
TTAACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC114820.16419912740791184No Hit
TTAACTGGGGCTTCCCAGCCCAGAGCTCGCCCAGACTTCCACACGCGCACGG105180.15041337938307062No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT93940.1343395403997495No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC91740.1311934153318397No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCT82390.11782238379322293No Hit
TTAACTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC81560.1166354366085115No Hit
TTAACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG78850.11275998254758621No Hit
TTAACTGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT74320.10628182502139007No Hit
TTAACTGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT74250.10618172104195657No Hit
TTAACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG73350.10489466987781165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTGG7204200.044.120532
TTAACTG7258450.044.1032031
AACTGGG7037900.044.0764473
ACTGGGA1874400.043.9054074
ACTGGGG4009100.043.8561024
ACTGGGC769400.043.737074
CTGGGGG1632800.043.4067275
ACTGGGT426350.043.237954
TGGGGGG820050.043.004576
CTGGGAT530450.042.9171455
CTGGGGA1042100.042.8129735
CTGGGAC369350.042.556385
CTGGGGC826200.042.5508355
TAACTAG30150.042.1094552
TGGGATA69500.041.962596
TGGGGAG479350.041.950356
CTGGGGT600200.041.949525
TGGGGGC386050.041.847436
TGGGGTC173050.041.6139876
TGGGGCT453950.041.6122936