##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527703_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6992729
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22308443527555	33.0	31.0	34.0	31.0	34.0
2	32.793511660469036	34.0	31.0	34.0	31.0	34.0
3	32.77909940453863	34.0	31.0	34.0	31.0	34.0
4	36.48446893909374	37.0	37.0	37.0	35.0	37.0
5	36.312579108957316	37.0	37.0	37.0	35.0	37.0
6	36.32670277941559	37.0	37.0	37.0	35.0	37.0
7	36.37849386126647	37.0	36.0	37.0	35.0	37.0
8	36.56014769055114	37.0	37.0	37.0	35.0	37.0
9	38.63770968387306	39.0	39.0	39.0	38.0	39.0
10	37.996374090859234	39.0	38.0	39.0	37.0	39.0
11	37.7641501622614	39.0	38.0	39.0	35.0	39.0
12	37.237382143652354	39.0	37.0	39.0	35.0	39.0
13	36.968839633281945	39.0	37.0	39.0	33.0	39.0
14	37.83115833603733	40.0	37.0	40.0	33.0	40.0
15	37.916757248850914	40.0	37.0	40.0	34.0	40.0
16	37.99085836159245	40.0	37.0	40.0	34.0	40.0
17	37.90011238816777	40.0	37.0	40.0	33.0	40.0
18	37.903763323303394	40.0	37.0	40.0	34.0	40.0
19	37.86758174097695	40.0	37.0	40.0	34.0	40.0
20	37.85173542403831	40.0	37.0	40.0	33.0	40.0
21	37.90167987348001	40.0	37.0	40.0	34.0	40.0
22	37.79842776689902	40.0	37.0	40.0	34.0	40.0
23	37.81301420375364	40.0	37.0	40.0	34.0	40.0
24	37.80991226744237	40.0	37.0	40.0	34.0	40.0
25	37.76551872666594	40.0	37.0	40.0	34.0	40.0
26	37.6027759691531	40.0	36.0	40.0	34.0	40.0
27	37.496651593390794	40.0	36.0	40.0	33.0	40.0
28	37.374586087920754	40.0	36.0	40.0	33.0	40.0
29	37.3084553970274	40.0	35.0	40.0	33.0	40.0
30	37.15029768778398	40.0	35.0	40.0	33.0	40.0
31	37.07363806033381	39.0	35.0	40.0	32.0	40.0
32	37.01839081709015	39.0	35.0	40.0	32.0	40.0
33	36.721924873679505	39.0	35.0	40.0	31.0	40.0
34	36.526057423360754	39.0	35.0	40.0	31.0	40.0
35	36.51733979680894	39.0	35.0	40.0	31.0	40.0
36	36.389896419552365	39.0	35.0	40.0	31.0	40.0
37	36.40518058114364	39.0	35.0	40.0	31.0	40.0
38	36.05753747928741	38.0	35.0	40.0	30.0	40.0
39	36.0960491962437	38.0	35.0	40.0	30.0	40.0
40	35.94371281941571	38.0	35.0	40.0	30.0	40.0
41	35.59221442730013	38.0	35.0	40.0	29.0	40.0
42	35.64764571886026	38.0	35.0	40.0	29.0	40.0
43	35.6022767363071	38.0	35.0	40.0	29.0	40.0
44	35.45249730112521	38.0	34.0	40.0	29.0	40.0
45	35.31483859877882	37.0	34.0	40.0	28.0	40.0
46	34.97216136933091	37.0	34.0	40.0	27.0	40.0
47	34.70035432518549	37.0	33.0	40.0	26.0	40.0
48	34.6900913792026	36.0	33.0	40.0	26.0	40.0
49	34.63219138050395	36.0	33.0	40.0	27.0	40.0
50	34.51693451869792	36.0	33.0	40.0	27.0	40.0
51	34.794946579511375	36.0	34.0	40.0	28.0	40.0
52	34.534540234577946	35.0	34.0	40.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	3.0
13	7.0
14	18.0
15	48.0
16	135.0
17	386.0
18	987.0
19	1859.0
20	3464.0
21	6178.0
22	9811.0
23	14853.0
24	21686.0
25	30292.0
26	42018.0
27	55907.0
28	74788.0
29	98199.0
30	126866.0
31	160343.0
32	208307.0
33	289956.0
34	543821.0
35	559132.0
36	664699.0
37	1095759.0
38	2051967.0
39	931236.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.54049413326328	0.38328383668235966	0.06405224626894593	0.012169783785414822
8	99.26131843519175	0.6441691076545366	0.06034839902990663	0.0341640581238026
9	97.5578061154665	1.2818028555089152	0.4599920860653974	0.7003989429591795
10	56.155872764410006	26.651740686647518	6.134471963663971	11.057914585278509
11	37.91042095296414	23.190044973857844	19.40218189493687	19.497352178241144
12	32.51760507235444	16.265881317580018	25.83506382129209	25.381449788773452
13	26.16201771869037	15.70391187760887	29.01991482867419	29.114155575026572
14	25.02899797775661	16.663651630143253	31.748391793818982	26.55895859828116
15	26.410432893938836	18.054296112433356	29.287850279912174	26.24742071371563
16	31.49422779003734	18.803645901335518	25.90721018932666	23.794916119300492
17	31.404820635834735	19.29685820800434	23.92294910899593	25.37537204716499
18	30.86493127361292	19.924023939723675	24.246242060860645	24.96480272580276
19	29.55209618447962	21.595846199673975	23.33020484563323	25.521852770213176
20	30.218230965335565	21.962169562126604	23.889843292940423	23.92975617959741
21	30.064142339850434	21.888392929284116	25.132791503860652	22.91467322700479
22	28.841829849261995	20.31747548060278	26.84431214194058	23.996382528194644
23	30.597138828059833	20.186010354469623	25.141757960304194	24.07509285716635
24	31.42585677208426	20.411201406489514	22.63584074257704	25.527101078849185
25	28.806907861008195	20.37971155467343	22.904119407458804	27.909261176859562
26	26.764643674880006	19.977736874973992	25.01835835479968	28.239261095346325
27	25.916319651455105	19.121104221256108	25.068267338831518	29.89430878845727
28	27.27229955572424	22.10793525675026	22.955315442654793	27.664449744870705
29	30.243929086912992	21.141116722813084	22.54846426910009	26.066489921173837
30	29.456468282983657	19.869538773774874	25.40457380802259	25.269419135218886
31	31.879528006876857	19.702522434374334	24.18073115660567	24.23721840214314
32	33.91996172023826	19.686791809034784	22.66242549940088	23.73082097132607
33	33.55382426517601	19.572072648604	23.444966907769484	23.429136178450502
34	30.31776292202944	21.369739911270695	27.30554837746465	21.00694878923522
35	28.993616083220157	25.26581539195928	25.33331407523443	20.407254449586134
36	29.00877468582009	25.819304594815556	22.649111670136225	22.522809049228133
37	30.493002088312014	25.178896536674024	21.360015524697154	22.968085850316807
38	30.376724165915768	24.61928669050381	21.938244711042	23.065744432538427
39	30.767115385137906	22.428554002307255	21.79277932835664	25.0115512841982
40	29.069137385418482	22.399366542018146	21.578985829423676	26.95251024313969
41	26.462015044484062	23.78313245086432	22.61714989955996	27.13770260509166
42	24.835725794607512	21.758500865684912	23.968038801446472	29.4377345382611
43	24.654980909456093	20.757003453158273	24.70215848490625	29.885857152479385
44	26.759338163970032	19.421530564104515	24.16042434934916	29.658706922576293
45	28.93642810982665	20.59537842807865	23.00133467205722	27.46685879003748
46	27.53756080065451	23.95877203306463	22.521722206022858	25.98194496025801
47	27.122100684868528	22.39523367772439	24.092468047882306	26.390197589524778
48	27.64023888241629	21.231095899755303	25.69448923303048	25.434175984797925
49	29.306841434867557	21.355768255855477	23.665567477303924	25.67182283197304
50	25.909970199045322	21.96544439231093	23.852890623960974	28.271694784682776
51	24.833695113881863	22.00016617260586	25.292814865269335	27.873323848242936
52	25.003700272096918	21.91690826285417	25.495239412252356	27.584152052796558
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	31.5
2	63.0
3	52.0
4	41.0
5	236.5
6	432.0
7	1902.0
8	3372.0
9	7774.5
10	12177.0
11	18191.0
12	24205.0
13	21678.0
14	19719.5
15	20288.0
16	21270.5
17	22253.0
18	21910.5
19	21568.0
20	23059.0
21	24550.0
22	27785.5
23	31021.0
24	35861.5
25	40702.0
26	44555.5
27	48409.0
28	55354.0
29	62299.0
30	67474.5
31	72650.0
32	79926.0
33	87202.0
34	99596.0
35	111990.0
36	123972.5
37	135955.0
38	137661.5
39	149905.5
40	160443.0
41	176764.5
42	193086.0
43	207594.0
44	222102.0
45	243183.5
46	264265.0
47	279807.0
48	295349.0
49	311362.5
50	327376.0
51	359999.5
52	392623.0
53	455642.5
54	518662.0
55	559276.5
56	599891.0
57	650993.0
58	702095.0
59	709596.5
60	717098.0
61	621467.5
62	525837.0
63	458253.0
64	325720.0
65	260771.0
66	225268.5
67	189766.0
68	165940.5
69	142115.0
70	126459.5
71	110804.0
72	86921.5
73	63039.0
74	43190.0
75	23341.0
76	16335.5
77	9330.0
78	7012.5
79	4695.0
80	2926.0
81	1157.0
82	728.0
83	299.0
84	229.5
85	160.0
86	100.5
87	41.0
88	28.0
89	9.5
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	6992729.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.067274499466768
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.38821283208001	5.597737599438119
2	9.8602174898915	1.5909016223079584
3	4.156900612986972	1.0060457493590274
4	2.168781232924602	0.6998461414117896
5	1.233038797049855	0.49736312221467377
6	0.8078867497827873	0.3910466504987862
7	0.5085071952987367	0.2871586990600206
8	0.35379392044468255	0.22833221379758095
9	0.3112708591736672	0.22599967181749528
>10	4.899853113596734	10.973120260320052
>50	3.610729536018626	20.91757358183314
>100	2.5422781302639157	36.869737409870304
>500	0.10259971949815605	5.5805811739358475
>1k	0.049633077302357075	7.568017603601683
>5k	0.0031483668438062325	1.7107246324424703
>10k+	0.0031483668438062325	5.855813868091067
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	85289	1.2196811859861867	No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	45742	0.6541366038924145	No Hit
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	34177	0.4887505292997913	No Hit
TTAACTGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC	25872	0.36998430798619536	No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	25582	0.3658371431239506	No Hit
TTAACTGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCT	21994	0.31452670338003946	No Hit
TTAACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	19757	0.28253633166679276	No Hit
TTAACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	17182	0.2457123678037573	No Hit
TTAACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	15586	0.22288866049292058	No Hit
TTAACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	15424	0.2205719683974597	No Hit
TTAACTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	13728	0.19631820423757304	No Hit
TTAACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	13167	0.188295585314403	No Hit
TTAACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	12422	0.1776416617889811	No Hit
TTAACTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	12035	0.1721073417831579	No Hit
TTAACTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	11987	0.17142091449561395	No Hit
TTAACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	11482	0.16419912740791184	No Hit
TTAACTGGGGCTTCCCAGCCCAGAGCTCGCCCAGACTTCCACACGCGCACGG	10518	0.15041337938307062	No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	9394	0.1343395403997495	No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	9174	0.1311934153318397	No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCT	8239	0.11782238379322293	No Hit
TTAACTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	8156	0.1166354366085115	No Hit
TTAACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	7885	0.11275998254758621	No Hit
TTAACTGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT	7432	0.10628182502139007	No Hit
TTAACTGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT	7425	0.10618172104195657	No Hit
TTAACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	7335	0.10489466987781165	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.017332289010485034	0.0	0.0	0.0	0.0
8	0.021851268653482782	0.0	0.0	0.0	0.0
9	0.024411070413282138	0.0	0.0	0.0	0.0
10	0.03728158205473142	0.0	0.0	0.0	0.0
11	0.05085282155221516	0.0	0.0	0.0	0.0
12	0.06225037463914303	0.0	0.0	0.0	0.0
13	0.06975817309665511	0.0	0.0	0.0	0.0
14	0.0771801681432242	0.0	0.0	0.0	0.0
15	0.08551739957318523	0.0	0.0	0.0	0.0
16	0.09670044413275561	0.0	0.0	0.0	0.0
17	0.107626078459497	0.0	0.0	0.0	0.0
18	0.11733616446454596	0.0	0.0	0.0	0.0
19	0.13050698804429572	0.0	0.0	0.0	0.0
20	0.14121811384367963	0.0	0.0	0.0	0.0
21	0.1507708935953331	0.0	0.0	0.0	0.0
22	0.1597087489018951	0.0	0.0	0.0	0.0
23	0.17079168948203197	0.0	0.0	0.0	0.0
24	0.18094509311028642	0.0	0.0	0.0	0.0
25	0.19115569901250284	0.0	0.0	0.0	0.0
26	0.2022815412981112	0.0	0.0	0.0	0.0
27	0.21287826254957112	0.0	0.0	0.0	0.0
28	0.2263207969306404	0.0	0.0	0.0	0.0
29	0.23630259373700882	0.0	0.0	0.0	0.0
30	0.24795755705676567	0.0	0.0	0.0	0.0
31	0.2576819436303051	0.0	0.0	0.0	0.0
32	0.2673491279298826	0.0	0.0	0.0	0.0
33	0.27763123667455153	0.0	0.0	0.0	0.0
34	0.2876273340494105	0.0	0.0	0.0	0.0
35	0.3015560877591567	0.0	0.0	0.0	0.0
36	0.31439799826362497	0.0	0.0	0.0	0.0
37	0.3275831224118652	0.0	0.0	0.0	0.0
38	0.33942399312199856	0.0	0.0	0.0	0.0
39	0.3505641359760975	0.0	0.0	0.0	0.0
40	0.36184728451510134	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACTGG	720420	0.0	44.12053	2
TTAACTG	725845	0.0	44.103203	1
AACTGGG	703790	0.0	44.076447	3
ACTGGGA	187440	0.0	43.905407	4
ACTGGGG	400910	0.0	43.856102	4
ACTGGGC	76940	0.0	43.73707	4
CTGGGGG	163280	0.0	43.406727	5
ACTGGGT	42635	0.0	43.23795	4
TGGGGGG	82005	0.0	43.00457	6
CTGGGAT	53045	0.0	42.917145	5
CTGGGGA	104210	0.0	42.812973	5
CTGGGAC	36935	0.0	42.55638	5
CTGGGGC	82620	0.0	42.550835	5
TAACTAG	3015	0.0	42.109455	2
TGGGATA	6950	0.0	41.96259	6
TGGGGAG	47935	0.0	41.95035	6
CTGGGGT	60020	0.0	41.94952	5
TGGGGGC	38605	0.0	41.84743	6
TGGGGTC	17305	0.0	41.613987	6
TGGGGCT	45395	0.0	41.612293	6
>>END_MODULE