FastQCFastQC Report
Fri 17 Jun 2016
SRR1527702_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527702_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893902
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC50450.564379540486541No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG33290.3724121883606928No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC32670.3654763050088265No Hit
TAGAGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27570.30842307098541005No Hit
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGAGAGGGA20690.23145713959695804No Hit
TAGAGAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG19320.21613107477105992No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG16830.18827567227727424No Hit
TAGAGAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA16730.18715698141407No Hit
TAGAGAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC15910.1779837163357952No Hit
TAGAGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA14480.16198643699197451No Hit
TAGAGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT12860.14386364500806575No Hit
TAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12540.14028383424581217No Hit
TAGAGAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA12430.1390532742962875Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA12320.13782271434676285Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA12320.13782271434676285No Hit
TAGAGAGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA11680.1306630928222557Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAG11350.12697141297368167No Hit
TAGAGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA11140.12462216216095276Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC10880.12171356591662173No Hit
TAGAGAAGGGGAGTTTTCCTGCAAGTTGAGTAGCGCCAAGGCCGCTGCAG10560.11813375515436816No Hit
TAGAGAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA10250.11466581347843499Illumina Single End Adapter 2 (97% over 34bp)
TAGAGAGGGGCTTTTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAGC9440.10560441748648061No Hit
TAGAGAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTT9030.10101778494734323No Hit
TAGAGAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC8970.10034657042942068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAAAC600.044.00000423
CGACAAT302.527082E-644.00000410
CCGTACC302.527082E-644.00000417
CGACTTA302.527082E-644.00000421
TATACTA302.527082E-644.00000444
CGTCACA600.044.00000444
GCCGTAC302.527082E-644.00000416
TGATCGC207.855542E-444.033
TCCATTA254.442044E-544.028
CAAACGT207.855542E-444.036
TTACTAC207.855542E-444.027
TACGGAC207.855542E-444.026
AGTATCG351.4456054E-744.017
GCCGATA207.855542E-444.040
TACGCGG254.442044E-544.031
TACGACA207.855542E-444.039
ACGCTTA207.855542E-444.023
ACCAATT254.442044E-544.012
AATAGTA207.855542E-444.015
ATACGGA207.855542E-444.025
Produced by FastQC (version 0.11.5)