##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527702_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	893902
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30835594953362	33.0	31.0	34.0	31.0	34.0
2	32.489364605963516	34.0	31.0	34.0	31.0	34.0
3	32.858511335694516	34.0	31.0	34.0	31.0	34.0
4	35.993915440395035	37.0	35.0	37.0	35.0	37.0
5	36.27446185376026	37.0	37.0	37.0	35.0	37.0
6	35.87225445294898	37.0	35.0	37.0	35.0	37.0
7	36.463294634087404	37.0	37.0	37.0	35.0	37.0
8	36.67723307476658	37.0	37.0	37.0	35.0	37.0
9	38.72953411000311	39.0	39.0	39.0	39.0	39.0
10	38.18731695420751	39.0	39.0	39.0	37.0	39.0
11	37.93619546661714	39.0	38.0	39.0	35.0	39.0
12	37.61956679815013	39.0	37.0	39.0	35.0	39.0
13	37.546870909786534	39.0	37.0	39.0	35.0	39.0
14	38.27555033997015	40.0	38.0	40.0	35.0	40.0
15	38.37879543842614	40.0	38.0	40.0	35.0	40.0
16	38.387345592693606	40.0	38.0	40.0	35.0	40.0
17	38.36727739729859	40.0	38.0	40.0	35.0	40.0
18	38.273632903830624	40.0	38.0	40.0	35.0	40.0
19	38.25403232121642	40.0	38.0	40.0	34.0	40.0
20	38.14654514700717	40.0	38.0	40.0	34.0	40.0
21	38.14597685204866	40.0	38.0	40.0	34.0	40.0
22	38.14886978662091	40.0	38.0	40.0	34.0	40.0
23	38.06526666234106	40.0	38.0	40.0	34.0	40.0
24	37.9258173714792	40.0	38.0	40.0	34.0	40.0
25	37.69226492389546	40.0	37.0	40.0	34.0	40.0
26	37.53142067027482	40.0	36.0	40.0	33.0	40.0
27	37.35273777214952	40.0	36.0	40.0	33.0	40.0
28	37.20470700367602	39.0	35.0	40.0	33.0	40.0
29	37.154653418383674	40.0	35.0	40.0	33.0	40.0
30	37.1920691529944	40.0	35.0	40.0	33.0	40.0
31	37.18042693718103	40.0	35.0	40.0	33.0	40.0
32	37.10083431964578	40.0	35.0	40.0	33.0	40.0
33	36.94527252428119	40.0	35.0	40.0	33.0	40.0
34	36.57188819356037	40.0	35.0	40.0	31.0	40.0
35	36.472385116041806	40.0	35.0	40.0	31.0	40.0
36	36.26747674801041	40.0	35.0	40.0	30.0	40.0
37	35.894319511534825	39.0	35.0	40.0	29.0	40.0
38	35.650947195553876	39.0	35.0	40.0	27.0	40.0
39	35.344642925063376	39.0	35.0	40.0	26.0	40.0
40	35.015788084152405	39.0	35.0	40.0	23.0	40.0
41	34.767513664808895	38.0	35.0	40.0	23.0	40.0
42	34.602147662719176	38.0	35.0	40.0	23.0	40.0
43	34.394491789927756	38.0	34.0	40.0	22.0	40.0
44	34.03661139588009	37.0	34.0	40.0	20.0	40.0
45	33.91546948099456	37.0	34.0	40.0	20.0	40.0
46	33.73233866799716	36.0	34.0	40.0	20.0	40.0
47	33.56326532438679	36.0	33.0	40.0	19.0	40.0
48	33.41287747426452	36.0	33.0	40.0	18.0	40.0
49	33.29888399399487	35.0	33.0	40.0	18.0	40.0
50	32.70972321350663	35.0	33.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	5.0
14	8.0
15	14.0
16	36.0
17	83.0
18	185.0
19	362.0
20	641.0
21	1094.0
22	1880.0
23	3110.0
24	4627.0
25	6533.0
26	8702.0
27	11978.0
28	16276.0
29	19370.0
30	21132.0
31	20751.0
32	22077.0
33	26868.0
34	46072.0
35	57634.0
36	81597.0
37	143890.0
38	263798.0
39	135177.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.05000771896695	0.6361994939042535	0.24957993158086683	0.06421285554792361
8	98.44580278375035	1.094191533300071	0.19912697365035542	0.2608787092992297
9	97.26625513758779	1.524663777461064	0.6649498490886026	0.5441312358625442
10	58.34397954138149	26.57080977556824	5.919664571731577	9.16554611131869
11	31.704034670467234	30.367870303456083	18.04593792160662	19.882157104470068
12	28.493391893071053	22.59900973484789	26.019295180008545	22.88830319207251
13	23.110475197504872	23.491725043684877	27.310040697973605	26.087759060836646
14	22.919067190810626	25.910670297191412	30.310593331260026	20.85966918073793
15	25.69845464044157	23.565782378829	27.855738101044636	22.8800248796848
16	26.8143487764878	25.30221433669463	24.558844258095405	23.324592628722165
17	29.560734845654224	25.043013663690207	21.690968361185007	23.70528312947057
18	26.883595740920146	25.86279032824627	22.322245615291163	24.93136831554242
19	28.893659483925532	26.00620649690906	21.26127920062826	23.838854818537154
20	26.331186192669893	27.465986204304276	22.356925032050494	23.84590257097534
21	26.451781067723307	26.22099514264427	24.715796586202963	22.61142720342946
22	27.24560410425304	24.998266029162032	23.48422981490141	24.271900051683517
23	27.18329302317256	26.93192318621057	21.571044700649512	24.313739089967356
24	27.958881398632062	26.485565531792076	21.839642376904848	23.71591069267101
25	27.31977330848348	27.302545469190136	20.224588377696882	25.153092844629498
26	28.615217328073996	26.00508780604585	20.759993824826434	24.61970104105372
27	26.570138561050317	25.531545963651496	21.48591232595967	26.41240314933852
28	28.567672966387814	26.961792232258126	19.7547382151511	24.715796586202963
29	30.148942501527014	26.7141140751447	20.113054898635422	23.02388852469286
30	28.622936295030105	26.118858666833724	21.31822056556535	23.93998447257082
31	29.669359728471356	27.36329038306213	19.3751664052659	23.592183483200618
32	30.811878707061847	25.589717888538114	20.518020991115357	23.080382413284678
33	30.76243257090822	25.478743754908255	20.5029186644621	23.255905009721424
34	27.94500963192833	28.476052184691387	22.186548413584486	21.392389769795795
35	29.246606451266473	28.694085033929895	21.53927388013451	20.520034634669123
36	29.458822108016314	28.410161292848656	20.880924307138816	21.250092291996214
37	28.780336099482938	28.376153090607247	21.18297084020396	21.66053996970585
38	30.570465218782374	26.99926837617547	20.22503585404217	22.205230550999996
39	28.786824506489527	26.062364778241907	19.4797640009755	25.671046714293066
40	29.002619974001625	26.851824920405143	20.81671145159089	23.32884365400234
41	27.793762627223117	26.627863009591657	21.80831903273513	23.770055330450095
42	26.15130070186665	26.559063521504594	22.241476134967815	25.048159641660945
43	28.403672885842074	25.438582752919224	22.35133157773447	23.806412783504232
44	27.32984152625232	25.513087564408625	21.370910905222274	25.78616000411678
45	26.87598864305036	25.967052316696908	22.005096755572758	25.151862284679975
46	26.719707529460724	27.191459466473955	20.8874127141454	25.201420289919923
47	25.68223362292511	27.286548189846314	23.174576183966476	23.856642003262102
48	25.87722144038161	27.69576530760643	22.393953699622553	24.033059552389414
49	27.415309508201126	27.997588102498934	21.208588860971336	23.37851352832861
50	24.817373716581905	28.2324013146855	22.103653420621054	24.846571548111537
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	28.5
4	57.0
5	69.0
6	81.0
7	112.5
8	144.0
9	1911.0
10	3678.0
11	3437.0
12	3196.0
13	3254.0
14	3312.0
15	3217.5
16	3123.0
17	3261.5
18	3400.0
19	4003.5
20	4607.0
21	4977.5
22	5348.0
23	7179.5
24	9011.0
25	9895.5
26	10780.0
27	11633.0
28	12486.0
29	12687.5
30	12889.0
31	14218.0
32	15547.0
33	16962.5
34	18378.0
35	17525.5
36	16673.0
37	18179.5
38	19686.0
39	21422.0
40	23158.0
41	24284.5
42	25411.0
43	27329.5
44	29248.0
45	31495.5
46	33743.0
47	36908.5
48	40074.0
49	40351.0
50	40628.0
51	45720.5
52	50813.0
53	54834.0
54	58855.0
55	62155.5
56	65456.0
57	68989.5
58	72523.0
59	70576.0
60	68629.0
61	64448.5
62	60268.0
63	56768.5
64	53269.0
65	48269.5
66	43270.0
67	35913.0
68	28556.0
69	27130.5
70	25705.0
71	20370.5
72	15036.0
73	12047.5
74	9059.0
75	6977.0
76	4895.0
77	3222.0
78	1549.0
79	1302.5
80	1056.0
81	633.5
82	211.0
83	127.0
84	43.0
85	43.5
86	44.0
87	23.5
88	3.0
89	3.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	893902.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.884946504726265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.43652753410984	12.568567854110283
2	9.282533598651762	3.134701664831183
3	3.5583400193289547	1.8024714261598807
4	1.7451812809202965	1.1786917027755546
5	1.0096905837479324	0.8524285746454835
6	0.6491263428367341	0.6576278144164114
7	0.5145171205019023	0.6081315838808291
8	0.39079118118582645	0.5278790551073175
9	0.2890774201428758	0.4392951097353062
>10	5.351424759600258	24.839625711027434
>50	1.9535872496695115	22.588012232593073
>100	0.7688007614798491	22.498029193552938
>500	0.03628954643348938	4.034939325634483
>1k	0.013440572753144213	3.697134146287093
>5k	6.720286376572107E-4	0.5724646052427224
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	5045	0.564379540486541	No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG	3329	0.3724121883606928	No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3267	0.3654763050088265	No Hit
TAGAGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2757	0.30842307098541005	No Hit
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGAGAGGGA	2069	0.23145713959695804	No Hit
TAGAGAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1932	0.21613107477105992	No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1683	0.18827567227727424	No Hit
TAGAGAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA	1673	0.18715698141407	No Hit
TAGAGAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC	1591	0.1779837163357952	No Hit
TAGAGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	1448	0.16198643699197451	No Hit
TAGAGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	1286	0.14386364500806575	No Hit
TAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1254	0.14028383424581217	No Hit
TAGAGAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA	1243	0.1390532742962875	Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	1232	0.13782271434676285	Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA	1232	0.13782271434676285	No Hit
TAGAGAGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	1168	0.1306630928222557	Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAG	1135	0.12697141297368167	No Hit
TAGAGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	1114	0.12462216216095276	Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	1088	0.12171356591662173	No Hit
TAGAGAAGGGGAGTTTTCCTGCAAGTTGAGTAGCGCCAAGGCCGCTGCAG	1056	0.11813375515436816	No Hit
TAGAGAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	1025	0.11466581347843499	Illumina Single End Adapter 2 (97% over 34bp)
TAGAGAGGGGCTTTTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAGC	944	0.10560441748648061	No Hit
TAGAGAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTT	903	0.10101778494734323	No Hit
TAGAGAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC	897	0.10034657042942068	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.10896049007609335	0.0	0.0	0.0	0.0
5	0.10896049007609335	0.0	0.0	0.0	0.0
6	0.32307792129338564	0.0	0.0	0.0	0.0
7	0.32688147022828007	0.0	0.0	0.0	0.0
8	0.4308078514199543	0.0	0.0	0.0	0.0
9	0.5927942884119288	0.0	0.0	0.0	0.0
10	0.772903517387812	0.0	0.0	0.0	0.0
11	1.071258370604384	0.0	0.0	0.0	0.0
12	1.1609773778333643	0.0	0.0	0.0	0.0
13	1.2293293895751436	0.0	0.0	0.0	0.0
14	1.3043935464961482	0.0	0.0	0.0	0.0
15	1.3879597539775053	0.0	0.0	0.0	0.0
16	1.4534031694749536	0.0	0.0	0.0	0.0
17	1.5173922868502363	0.0	0.0	0.0	0.0
18	1.582164487829762	0.0	0.0	0.0	0.0
19	1.6485028560177737	0.0	0.0	0.0	0.0
20	1.7204346785218065	0.0	0.0	0.0	0.0
21	1.794603882752248	0.0	0.0	0.0	0.0
22	1.8687730869826893	0.0	0.0	0.0	0.0
23	1.9545766761904548	0.0	0.0	0.0	0.0
24	2.047204279663766	0.0	0.0	0.0	0.0
25	2.1379301086696305	0.0	0.0	0.0	0.0
26	2.2331307011283115	0.0	0.0	0.0	0.0
27	2.3200529811992814	0.0	0.0	0.0	0.0
28	2.422748802441431	0.0	0.0	0.0	0.0
29	2.488080348852559	0.0	0.0	0.0	0.0
30	2.564039458464127	0.0	0.0	0.0	0.0
31	2.6174010126389695	0.0	0.0	0.0	0.0
32	2.673447424885502	0.0	0.0	0.0	0.0
33	2.72032057205376	0.0	0.0	0.0	0.0
34	2.7703260536389895	0.0	0.0	0.0	0.0
35	2.8401323635029345	0.0	0.0	0.0	0.0
36	2.8867817724985514	0.0	0.0	0.0	0.0
37	2.931865014285682	0.0	0.0	0.0	0.0
38	2.981087412266669	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTAAAC	60	0.0	44.000004	23
CGACAAT	30	2.527082E-6	44.000004	10
CCGTACC	30	2.527082E-6	44.000004	17
CGACTTA	30	2.527082E-6	44.000004	21
TATACTA	30	2.527082E-6	44.000004	44
CGTCACA	60	0.0	44.000004	44
GCCGTAC	30	2.527082E-6	44.000004	16
TGATCGC	20	7.855542E-4	44.0	33
TCCATTA	25	4.442044E-5	44.0	28
CAAACGT	20	7.855542E-4	44.0	36
TTACTAC	20	7.855542E-4	44.0	27
TACGGAC	20	7.855542E-4	44.0	26
AGTATCG	35	1.4456054E-7	44.0	17
GCCGATA	20	7.855542E-4	44.0	40
TACGCGG	25	4.442044E-5	44.0	31
TACGACA	20	7.855542E-4	44.0	39
ACGCTTA	20	7.855542E-4	44.0	23
ACCAATT	25	4.442044E-5	44.0	12
AATAGTA	20	7.855542E-4	44.0	15
ATACGGA	20	7.855542E-4	44.0	25
>>END_MODULE