FastQCFastQC Report
Fri 17 Jun 2016
SRR1527701_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527701_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336050
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA76500.5725833614011452No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC47330.3542532090864863No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT45670.3418285243815726No Hit
TAGAGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37270.2789566258747801No Hit
TAGAGAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA29100.2178062198271023No Hit
TAGAGAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA26720.19999251525017778No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT26240.1963998353355039No Hit
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGAGAGGGAAG26060.19505258036750123No Hit
TAGAGAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT24530.1836009131394783No Hit
TAGAGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC22800.17065229594700798No Hit
TAGAGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC19780.14804835148385165No Hit
TAGAGAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA18750.14033905916694733No Hit
TAGAGAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA18410.13779424422738668No Hit
TAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17840.1335279368287115No Hit
TAGAGAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG16520.12364806706335842No Hit
TAGAGAAGGGGAGTTTTCCTGCAAGTTGAGTAGCGCCAAGGCCGCTGCAGAA15800.11825904719134762No Hit
TAGAGAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC14520.10867856741888403No Hit
TAGAGAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA14210.10635829497399049Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGGCTTTTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAGCTC14180.10613375247932337No Hit
TAGAGAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA13670.10231653006998241Illumina Single End Adapter 2 (100% over 34bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAT253.4174554E-546.038
AACGTTG206.311825E-446.038
AACGTCG253.4174554E-546.017
AACGTAC253.4174554E-546.044
CGAAATC206.311825E-446.020
TAGGTTA206.311825E-446.020
GTTAGCA206.311825E-446.032
TAACGGC301.861632E-646.024
GCGTTAG453.110472E-1046.023
TCGATAT301.861632E-646.020
AGTATCG206.311825E-446.017
TTATACG206.311825E-446.031
TACGCTA301.861632E-646.045
TGTTACG206.311825E-446.015
CGTACGC405.6134013E-946.043
CGATGTA253.4174554E-546.027
CACGTTA206.311825E-446.042
CGACTTA551.8189894E-1246.021
TATACGC206.311825E-446.032
TAAGCGC206.311825E-446.038
Produced by FastQC (version 0.11.5)